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Study of Phytochemical Screening and Antimicrobial Activity of Citrus aurantifolia Seed Extracts

American Journal of Analytical Chemistry 2016年第3期7卷 254-259页

作者： rehab mobark osman mohammed Saad Mohmmed Hussein Ayoub Department of Pharmacognosy Faculty of Pharmacy University of Al-Neelain Khartoum Sudan

Citrus aurantifolia, the family Rutaceae, which consists of 150 genera and 900 species, is gaining grounds as important source for treatment in complementary medicine, and commonly called limone. Chloroform, methanol and ethanol extract exhibited significant antimicrobial activity and highlighted the biological monitoring of activity from the methanol extract of seeds. Phytochemical screening of the diethyl ether, methanol and aqueous extracts of seeds confirmed the presence of alkaloids, saponins, sterols and triterpenes, carotenoids, coumarins, tannins and carbohydrates. Chloroform extract was fractionated by sing liquid solid column chromatographic technique and column fractions eluted with chloroform and methanol. The antibacterial secondary metabolites were achieved by fractionation of the active. The seed extracts were monitored analytically by TLC preparative and some chemical analyses of seeds have been done such as determination of ash, nitrogen, protein content.

关键词： C. aurantifolia Antimicrobial Activity Limone Phytochemical Screening Liquid Solid Column Chromatographic

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Pediatric Non-Hodgkin Lymphoma: A Retrospective 7-Year Experience in Children &Adolescents with Non-Hodgkin Lymphoma Treated in King Fahad Medical City (KFMC)

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Journal of Cancer Therapy 2015年第4期6卷 299-314页

作者： Nahla Ali mobark Suha A. Tashkandi Wafa Al Shakweer Khalid Al Saidi Suha A. Fataftah mohammed M. Al Nemer Awatif Alanazi mohammed Rayis Walid Ballourah Othman Mosleh Zaheer Ullah Fahad El Manjomi Musa Al Harbi Department of Pediatric Hematology and Oncology Comprehensive Cancer Centre KFMC Riyadh Saudi Arabia Department of Cytogenetic and Molecular Cytogenetic Laboratory KFMC Riyadh Saudi Arabia Department of Anatomical Pathology KFMC Riyadh Saudi Arabia

Background: Non-Hodgkin’s lymphoma is an aggressive malignant disease in children and adolescents. Although it is the fourth most common malignancy in Saudi children as reported in Saudi cancer registry, less information is available about pediatric Non-Hodgkin lymphoma and its outcome in Saudi Arabia. Study Objectives: To provide demographic data, disease characteristics, treatment protocol, toxicity and outcome of treatment in children & adolescents with Non-Hodgkin’s lymphoma treated at KFMC. This study will form base line for future studies about pediatric Non-Hodgkin’s lymphoma in KFMC, which may help to improve outcome for children with cancer in Saudi Arabia. Study Patients and Method: We retrospectively analyzed 28 children and adolescents diagnosed to have Non-Hodgkin’s lymphoma at KFMC between December 2006 and December 2013, followed-up through June 2014. Results: Of the 28 patients, 10 (35.7%) girls and 18 (64.3%) boys, the male-to-female ratio was 1.8;1. The median age at time of diagnosis was 6.4 years old (range 2.0 to 13.0 years old). The majority of patients (64.3%) were aged between 5 and 12 years old. Burkitt’s lymphoma BL/BLL was the most common pathological subtype (60.7%), and DLBCL was the second most common subtype (21.4%). Abdominal and Retroperitoneal involvement was the most common primary site (78.6%) including the ileocaecal region. Most of the children presented with advanced Stage III and IV (75%), Cytogenetic study which screens specifically for the t (8;14) (q24;q32) a characteristic genetic feature of Burkitt’s Lymphoma was obtained from 21 patients, variant rearrangement was observed in 3/21 samples and complex chromosomes karyotype in addition to IGH/MYC rearrangement was observed in 2/21 samples. Those patients presented with very aggressive lymphoma and combined BM and CNS involvement. We use the French-American-British Mature B-Cell Lymphoma 96 Protocol (FAB LMB 96) for treatment fornewly diagnosed Mature B-Cell type NHL and hi

关键词： Non-Hodgkin Lymphoma Disease Patients Children and Adolescents

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