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Potential Bacterial Health Risk Posed to Consumers of Fresh Coconut (<i>Cocos nucifera</i>L.) Water

Food and Nutrition Sciences 2012年第8期3卷 1136-1143页

作者： Adolf K. Awua Edna D. Doe rebecca agyare Cellular and Clinical Research Centre Radiological and Medical Sciences Research Institute Ghana Atomic Energy Commission Accra Ghana

Coconut (Cocos nucifera L.) water is a refreshing drink consumed mostly directly from the fruit. However, in recent times, consumers in Accra prefer to have it transferred into plastic bags for later consumption;this favours a high risk of bacterial contamination. Since it is rich in nutrient, it may become unwholesome with possible high bacteria loads. However, its use for managing and preventing diarrhoeal diseases and the report that coconut water contains anti-bacterial proteins, suggests a bacteria growth inhibition potential for it. Therefore, the propensity of fresh coconut water to support the growth of two pathogenic bacteria was studied. Using mostly optical density measurement, and where possible, growth parameters and bacteria loads were estimated for the growth of two gram negative bacteria in fresh, stored and sterilized coconut water, and also in Luria-Bertani (LB) broth as a control. The study revealed that fresh coconut water is a drink favourable for the survival and growth of Escherichia coli, and Klebsiella pneumoniae. It supported the growth of these bacteria recording lag times of 101.4 ± 1.00 minutes for E. coli and 154.8 ± 0.45 minutes for K. pneumoniae, and high loads of viable cells of ~ 2.27 × 108 cfu/mL and > 2.83 × 108 cfu/mL at the stationary phase for E. coli and K. pneumoniae respectively. These and other growth parameters in coconut water were comparable to those in Luria-Bertani (LB) broth medium. However, when autoclaved, gamma irradiated or stored at 4℃ for two weeks or more, the growth of these bacteria becomes extremely limited. Fresh coconut water will support the growth of these bacteria to high and infective load of viable cell if it becomes contaminated with and is kept at ambient temperatures for two or more hours. Thus, it will be safer to consume coconut water directly from the fruit, since there is a high risk for bacteria contamination associated with the transfer and storage in other containers.

关键词： Coconut Water Bacterial Growth Curves Health Risk

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Superior Conjugative Plasmids Delivered by Bacteria to Diverse Fungi

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BioDesign Research 2022年第1期2022卷 151-165页

作者： Ryan R.Cochrane Arina Shrestha Mariana M.Severo de Almeida Michelle agyare-Tabbi Stephanie L.Brumwell Samir Hamadache Jordyn S.Meaney Daniel P.Nucifora Henry Heng Say Jehoshua Sharma Maximillian P.M.Soltysiak Cheryl Tong Katherine Van Belois Emma J.L.Walker Marc-AndréLachance Gregory B.Gloor David R.Edgell rebecca S.Shapiro Bogumil J.Karas Department of Biochemistry Schulich School of Medicine and DentistryThe University of Western OntarioLondonONCanada N6A 5C1 Department of Molecular and Cellular Biology University of GuelphGuelphONCanada N1G 2W1 Department of Biology The University of Western OntarioLondonOntarioCanada N6A 5B7

Fungi are nature’s recyclers,allowing for ecological nutrient cycling and,in turn,the continuation of life on *** fungi inhabit the human microbiome where they can provide health benefits,while others are opportunistic pathogens that can cause ***,members of the fungal kingdom,have been domesticated by humans for the production of beer,bread,and,recently,medicine and ***,the great untapped potential exists within the diverse fungal ***,many yeasts are intractable,preventing their use in biotechnology or in the development of novel treatments for pathogenic ***,as a first step for the domestication of new fungi,an efficient DNA delivery method needs to be ***,we report the creation of superior conjugative plasmids and demonstrate their transfer via conjugation from bacteria to 7 diverse yeast species including the emerging pathogen Candida *** create our superior plasmids,derivatives of the 57 kb conjugative plasmid pTA-Mob 2.0 were built using designed gene deletions and insertions,as well as some unintentional ***,a cluster mutation in the promoter of the conjugative gene traJ had the most significant effect on improving conjugation to *** addition,we created Golden Gate assembly-compatible plasmid derivatives that allow for the generation of custom plasmids to enable the rapid insertion of designer genetic ***,we demonstrated that designer conjugative plasmids harboring engineered restriction endonucleases can be used as a novel antifungal agent,with important applications for the development of next-generation antifungal therapeutics.

关键词： insertion continuation harbor

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