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An oxygenating colloidal bioink for the engineering of biomimetic tissue constructs

Bio-Design and Manufacturing 2024年第3期7卷 240-261页

作者： Seol-Ha Jeong Jarno Hiemstra Patrick V.Blokzijl rebeca Damian-Ferrara Danilo Martins dos santos Jéssica H.L.da Fonseca Min-Ho Kang Jihyun Kim Dilara Yilmaz-Aykut Mei L.L.Cham-Pérez Jeroen Leijten Su Ryon Shin Division of Engineering in Medicine Department of MedicineBrigham and Women’s HospitalHarvard Medical SchoolBostonMA 02139USA Leijten Lab Department of Developmental BioEngineeringFaculty of Science and TechnologyTechnical Medical CentreUniversity of TwenteDrienerlolaan 57522 NB EnschedeThe Netherlands Nanotechnology National Laboratory for Agriculture(LNNA) Embrapa InstrumentaçãoSao CarlosSao Paulo 13560-970Brazil Department of Biomedical-Chemical Engineering The Catholic University of Korea43 Jibong-roBucheon-siGyeonggi-do 14662Republic of Korea Department of Biotechnology The Catholic University of Korea43 Jibong-roBucheon-siGyeonggi-do 14662Republic of Korea

Ensuring a sufficient oxygen supply is pivotal for the success of bioprinting applications since it fosters tissue integration and natural *** in oxygen concentration among diverse tissues necessitates the precise recreation of tissue-specific oxygen levels in imprinted constructs to support the survival of targeted *** oxygen-releasing biomaterials,such as oxygen-generating microparticles(OMPs),have shown promise for enhancing the oxygen supply of microenvironments in injured tissues,whether this approach is scalable for large tissues and whether tissue-specific bioinks with varying OMP concentrations remain printable remain *** study addresses this critical gap by introducing an innovative class of engineered oxygenated bioinks that combine colloidal-based microgels with *** report that incorporating nanosized calcium peroxide(nCaO_(2))and manganese oxide nanosheets(nMnO_(2))into hydrophobic polymeric microparticles enables precise modulation of oxygen release while controlling hydrogen peroxide ***,the fabrication of oxygenating and cytocompatible colloidal gels is achieved using an aqueous two-phase *** study thoroughly evaluates the fundamental characteristics of the resulting bioink,including its rheological behaviors,printability,shape fidelity,mechanical properties,and oxygen release ***,this study demonstrates the macroscopic scalability and cytocompatibility of printed constructs produced via cell-laden oxygenating colloidal *** showcasing the effectiveness of extrusion-based bioprinting,this study underscores how it can be used to fabricate biomimetic tissues,indicating its potential for new *** findings presented here advance the bioprinting field by achieving scalability with both high cell viability and the possibility of mimicking specifically oxygenated *** work thereby offers a promising avenue for the development of functional tissues with e

关键词： 3D bioprinting Bioink Colloidal gels Extrusion printing Oxygen-generating microparticle

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Effects of Gaseous Drinks in Wistar Rats Esophagus

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Journal of Biosciences and Medicines 2017年第5期5卷 32-43页

作者： Thiago Dornelas de Oliveira Marcelli Elioterio Gaspar Larissa Menezes Viana Braga Joice Meire Rodrigues rebeca nogueira falcao santos Suelen Gaudino Moura Nayara Barbosa Bicalho Lamara Laguardia Valente Rocha Daniel Almeida da Costa Marcus Vinicius de Mello Pinto Academic of the Graduate Course in Medicine of the University Center of Caratinga CaratingaBrazil Religious Sciences from the Pontifical Catholic University of Sao Paulo-PUC/SP Sao PauloBrazil University Center of Caratinga CaratingaBrazil The Institute of Health Sciences at UNEC CaratingaBrazil The Medical School at the Center of Higher Education of Valenca ValencaBrazil The Cellurare Institute Center of Laser Treatment and Cell Therapy PetropolisBrazil

The consumption of beverages and processed foods, mainly soft drinks, has been incorporated into the usual diet of children, adolescents and adults frequently in recent years. The present study aimed to study the effects of carbonated drinks on Wistar rats, based on the macroscopic and histological morphology of the esophagus. Fifteen Wistar rats were divided into three groups: control group;group fed with ration and cola-type refrigerant ad libitum and group fed with ration and gas industrialized water ad libitum, for a trial period of 90 days. For histopathology and morphology, the esophagus was removed, prepared in slides and stained with hematoxylineosin. Images of the sections were captured for analysis and classification of the inflammatory infiltrate. For morphometry, ten grid fields of 100 points were evaluated in the 40x eyepiece, totaling 3000 points per animal. The results demonstrate that the refrigerant treatment induced the following structural changes in the rats: significant reduction of weight in relation to the control group;inflammatory infiltrate predominantly diffuse mild to moderate and tissue edema. The rats treated with carbonated water had similar results to the control, besides signs of healing and tissue repair.

关键词： Soft Drinks Consumption Inflammation Carbonate Beverages

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A Numerical Study on Piezoelectric Energy Harvesting by Combining Transverse Galloping and Parametric Instability Phenomena

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Journal of Marine Science and Application 2017年第4期16卷 465-472页

作者： Guilherme Rosa Franzini rebeca Caramêz Saraiva santos Celso Pupo Pesce Escola PolitEnica University of SAO Paulo SAO Paulo SP 05508-030 Brazil

This paper aims to numerically investigate the effects of parametric instability on piezoelectric energy harvesting from the transverse galloping of a square prism. A two degrees-of-freedom reduced-order model for this problem is proposed and numerically integrated. A usual quasi-steady galloping model is applied, where the transverse force coefficient is adopted as a cubic polynomial function with respect to the angle of attack. Time-histories of nondimensional prism displacement, electric voltage and power dissipated at both the dashpot and the electrical resistance are obtained as functions of the reduced velocity. Both, oscillation amplitude and electric voltage, increased with the reduced velocity for all parametric excitation conditions tested. For low values of reduced velocity, 2:1 parametric excitation enhances the electric voltage. On the other hand, for higher reduced velocities, a 1:1 parametric excitation(i.e., the same as the natural frequency) enhances both oscillation amplitude and electric voltage. It has been also found that, depending on the parametric excitation frequency, the harvested electrical power can be amplified in 70% when compared to the case under no parametric excitation.

关键词： transverse galloping energy harvesting piezoelectricity parametric instability numerical simulations

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A review of superconducting fault current limiters compared with other proven technologies

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Superconductivity 2022年第3期3卷 55-72页

作者： Guilherme Gonçalves Sotelo Gabriel dos santos Felipe Sass Bruno Wanderley França Daniel Henrique nogueira Dias Marcio Zamboti Fortes Alexander Polasek Rubens de Andrade Jr. Fluminense Federal University(UFF) Electrical Engineering DepartmentNiteróiRio de JaneiroBrazil Electrical Energy Research Center(CEPEL) Rio de JaneiroRio de JaneiroBrazil Rio de Janeiro Federal University(UFRJ) Electrical Engineering DepartmentRio de JaneiroRio de JaneiroBrazil

Several substations in operation were commissioned decades *** substations are overstressed,then their protection equipment cannot provide an effective safety condition to the *** the worst cases,short‐circuits can cause permanent damage to the system if the overcurrent is higher than the capacity of the installed *** are some possible solutions for those cases:replace all the equipment in the overstressed substation,build a new one in parallel or install a fault current limiter(FCL)*** an economic point of view,introducing FCLs in the power system is the best way to solve the described *** commercial solutions available are the pyrotechnic FCL,air coil reactor,neutral earthing resistor,and high impedance ***,these devices are limited and present several *** the’70s,there has been a search for reliable FCL devices that do not interfere in the regular operation of substations and could limit the fault currents to the protection system rated *** were so many FCL technologies proposed in the last *** proposed new devices may use superconducting technologies,power electronics,or *** paper reviews proven FCL technologies,focusing on full‐scale devices demonstrated in field and lab *** goal is to introduce the main FCL technologies in development and discuss didactically their operation principle,the built prototypes,the commercial units,whether they exist,and the main drawbacks for each technology presented.A final analysis of the level of maturity for each FCL technology is discussed using the TRL(Technology readiness level)scale in order to find technologies with more potential for mass *** three technologies closer to the full commercial application are:the Resistive Superconducting FCL,the Saturated Iron Core FCL,and the Series Reactor FCL.

关键词： Fault current limiter Short circuit Superconductor Power system protection

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Technical Evaluation of Hybrid Clones of Corymbia spp. to Produce Market Pulp

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Paper And Biomaterials 2022年第3期7卷 1-6页

作者： Marcelo Moreira da Costa Ricardo de Carvalho Bittencourt Thales Augusto Pinto Coelho nogueira Larissa Soares Silva Weslley Henrique Martins da Silva Sebastião Renato Valverde Gleison Augusto Dos santos Daniel Alexander Fernandes Coelho Claudilene Aparecida Alves Pena Department of Forest Sciences Federal University of VicosaVicosaMinas Gerais36570900Brazil Aperam BioEnergia Co. CapelinhaMinas Gerais39680000Brazil

This study presents hybrid clones of Corymbia *** by Aperam BioEnergia as potential substitutes for Eucalyptus wood in the pulp *** biomass of Eucalyptus *** compared with that of Corymbia *** performing modified kraft pulping and basic density *** were made by analyzing their respective mean annual increments of cellulose(MAI_(cel))and specific wood consumption(SWC),estimated using a kappa number of(19±1).The results showed that one of the hybrid clones(Corymbia citriodora×Corymbia torelliana,ID 4)had higher basic wood density,higher screened yield,better MAI_(cel#k19),and lower SWC than the other samples,reaching values of 0.608 t/m^(3),54.1%,24.6 ADt/ha/year(i.e.,air dry ton/hectare/year),and 2.74 m^(3)/ADt,***,clone ID 4 had the highest MAI_(cel)/SWC ratio score(8.98).Given its high forest productivity and low industrial cost compared with other samples,as well as its advantages over Eucalyptus ***,we concluded that clone ID 4 has great potential as an alternative biomass for pulp production.

关键词： Corymbia hybrids modified kraft pulping mean annual increments of cellulose(MAIcel) specific wood consumption(SWC)

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Association of insertion-deletions polymorphisms with colorectal cancer risk and clinical features

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World Journal of Gastroenterology 2017年第37期23卷 6854-6867页

作者： Diego Marques Layse Raynara Ferreira-Costa Lorenna Larissa Ferreira-Costa Romualdo da Silva Correa Aline Maciel Pinheiro Borges Fernanda Ribeiro Ito Carlos Cesar de Oliveira Ramos Raul Hernandes Bortolin André Ducati Luchessi Andrea Ribeiro-dos-santos Sidney santos Vivian nogueira Silbiger Laboratório de Bioanálise e Biotecnologia Molecular Universidade Federal do Rio Grande do Norte Programa de Pós-graduacao em Ciências Farmacêutica Universidade Federal do Rio Grande do Norte Laboratório de Genética Humana e Médica Universidade Federal do Pará Departamento de Cirurgia Oncológica Liga Norte Riograndense Contra o Cancer Laboratório de Patologia eCitopatologia Liga Norte Riograndense Contra o Cancer Depart-amento de Análises Clínicas e Toxicológicas Universidade Federal do Rio Grande do Norte Núcleo de Pesquisas em Oncologia Universidade Federal do Pará

AIM To investigate the association between 16 insertiondeletions(INDEL) polymorphisms, colorectal cancer(CRC) risk and clinical features in an admixed *** O n e h u n d re d a n d fo r ty p a t i e n t s w i t h C R C a n d 140 cancer-free subjects were examined. Genomic DNA was extracted from peripheral blood samples. Polymorphisms and genomic ancestry distribution were assayed by Multiplex-PCR reaction, separated by capillary electrophoresis on the ABI 3130 Genetic Analyzer instrument and analyzed on Gene Mapper ID v3.2. Clinicopathological data were obtained by consulting the patients' clinical charts, intra-operative documentation, and pathology *** Logistic regression analysis showed that polymorphism variations in IL4 gene was associated with increased CRC risk, while TYMS and UCP2 genes were associated with decreased risk. Reference to anatomical localization of tumor Del allele of NFKB1 and CASP8 were associated with more colon related incidents than rectosigmoid. In relation to the INDEL association with tumor node metastasis(TNM) stage risk, the Ins alleles of ACE, HLAG and TP53(6 bp INDEL) were associated with higher TNM stage. Furthermore, regarding INDEL association with relapse risk, the Ins alleles of ACE, HLAG, and UGT1A1 were associated with early relapse risk, as well as the Del allele of TYMS. Regarding INDEL association with death risk before 10 years, the Ins allele of SGSM3 and UGT1A1 were associated with death *** The INDEL variations in ACE, UCP2, TYMS, IL4, NFKB1, CASP8, TP53, HLAG, UGT1A1, and SGSM3 were associated with CRC risk and clinical features in an admixed population. These data suggest that this cancer panel might be useful as a complementary tool for better clinical management, and more studies need to be conducted to confirm these findings.

关键词： Colorectal cancer Ins-del polymorphism Admixed population Potential biomarker Diagnostic Risk stratification Prognostic Clinical features

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Sagittal abdominal diameter as a marker of visceral obesity in older primary care patients

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Journal of Geriatric Cardiology 2020年第5期17卷 279-283页

作者： Maria Auxiliadora nogueira Saad Antonio José Lagoeiro Jorge Diane Xavier deávila Wolney de Andrade Martins Márcia Maria Sales dos santos Luciana Thurler Tedeschi Ismar Lima Cavalcanti Maria Luiza Garcia Rosa Rubens Antunes da Cruz Filho Fluminense Federal University NiteróiRio de Janeiro StateBrazil

Background Longevity,combined with a higher prevalence of obesity,particularly visceral obesity,has been associated with an increased risk of cardiovascular *** resistance(IR)is an important link between visceral obesity and cardiovascular *** important association has been found between sagittal abdominal diameter,visceral obesity and *** objective of this study is to evaluate sagittal abdominal diameter as a marker of visceral obesity and correlate it with IR in older primary health care *** A cross-sectional study was performed with 389 patients over 60 years of age(70.6±6.9),of whom 74%were *** clinical,anthropometric and metabolic profiles were assessed and their fasting serum insulin level was used to calculate the homeostasis model assessment insulin resistance(HOMA-IR).Sagittal abdominal diameter was measured in the supine position at the midpoint between the iliac crest and the last rib with abdominal *** Sagittal abdominal diameter was significantly correlated with anthropometric measures of general and visceral obesity and with HOMA-IR in both *** was no change in the association between sagittal abdominal diameter and HOMA-IR after adjusting for age,sex,diabetes and *** It is feasible to use sagittal abdominal diameter in older primary care patients as a tool to evaluate visceral obesity,which is an indicator of cardiovascular risk.

关键词： Cardiovascular risk Insulin resistance Primary health care Sagittal abdominal diameter

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Transmission of severe acute respiratory syndrome coronavirus 2 via fecal-oral:Current knowledge

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World Journal of Clinical Cases 2021年第28期9卷 8280-8294页

作者： Filipe Antônio França da Silva Breno Bittencourt de Brito Maria Luísa Cordeiro santos Hanna santos Marques Ronaldo Teixeira da Silva Júnior Lorena Sousa de Carvalho Samuel de Sousa Cruz Gabriel Reis Rocha Gabriel Lima Correa santos Kathlen Coutinho de Souza rebeca Gabrielle Almeida Maciel Daiana Silva Lopes Natália Oliveira e Silva Márcio Vasconcelos Oliveira Fabrício Freire de Melo Instituto Multidisciplinar em Saúde Universidade Federal da BahiaVitória da Conquista 45002175BahiaBrazil Departamento de Ciências Naturais Universidade Estadual do Sudoeste da BahiaVitória da Conquista 45002175BahiaBrazil Departamento de Bioquímica e Biofísica Universidade Federal da BahiaSalvador 40.110-100BahiaBrazil Instituto Multidisciplinar em Saúde Universidade Federal da BahiaVitória da Conquista 45029-094BahiaBrazil

The pandemic caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)has resulted in more than 93 million cases and 2 million deaths in the ***-CoV-2 respiratory tract infection and its main clinical manifestations such as cough and shortness of breath are well known to the scientific ***,a growing number of studies have reported SARS-CoV-2-related gastrointestinal involvement based on clinical manifestations,such as diarrhea,nausea,vomiting,and abdominal pain as well as on the pathophysiological mechanisms associated with coronavirus disease ***,current evidence suggests SARS-CoV-2 transmission via the fecal-oral route and aerosol ***,studies have shown a high risk of contamination through hospital surfaces and personal ***,viable SARS-CoV-2 specimens can be obtained from aerosols,which raises the possibility of transmission through aerosolized viral particles from ***,the infection by SARS-CoV-2 via fecal-oral route or aerosolized particles should *** addition,a possible viral spread to sources of drinking water,sewage,and rivers as well as the possible risk of viral transmission in shared toilets become a major public health concern,especially in the least developed *** authors have emphasized the presence of viral RNA and even viable SARS-CoV-2 in human feces,studies on the possible fecal-oral coronavirus disease 2019 transmission become essential to understand better the dynamics of its transmission and,then,to reinforce preventive measures against this infection,leading to a more satisfactory control of the incidence of the infection.

关键词： COVID-19 SARS-CoV-2,Transmission Via fecal-oral

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Research advances on the multiple uses of Moringa oleifera: a sustainable alternative for socially neglected population

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Asian Pacific Journal of Tropical Medicine 2017年第7期10卷 689-700页

作者： Raimunda Samia nogueira Brilhante Jamille Alencar Sales Vandbergue santos Pereira Débora de Souza Collares Maia Castelo-Branco Rossana de Aguiar Cordeiro Célia Maria de Souza Sampaio Manoel de Araújo Neto Paiva Joao Bosco Feitosa dos santos JoséJúlio CostaSidrim Marcos Fábio Gadelha Rocha Department of Pathology and Legal Medicine Postgraduate Program in Medical MicrobiologySpecialized Medical Mycology CenterFederal University of Ceará School of Veterinary Medicine Postgraduate Program in Veterinary SciencesState University of Ceará Department of Social Sciences Postgraduate Program of SociologyState University of Ceará

Moringa oleifera Lam(Moringaceae) is a plant with high nutritional and medicinal value. Native to India, it is now widely distributed throughout tropical and subtropical regions of the world. Its different parts are sources of proteins, vitamins and minerals and present different pharmacological and biotechnological potential. Moreover, M. oleifera seeds are widely used in water and effluent treatment, for their coagulation, flocculation and sedimentation properties, their ability of improving water quality, by reducing organic matter and microbial load, with special applicability in intensive animal production systems, such as aquaculture. In addition, due to its high nutritional value and several medicinal properties, this tree may act as a nutritional and medical alternative for socially neglected populations. In this context, this review gathers information on M. oleifera, emphasizing its chemical constituents, nutritional, pharmacological and antimicrobial properties, applications in the treatment of water effluents, and ecological and social aspects.

关键词： Moringa oleifera Biotechnology Nutrition Health Aquaculture

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Bioactive extracts of red seaweeds Pterocladiella capillacea and Osmundaria obtusiloba(Floridophyceae:Rhodophyta) with antioxidant and bacterial agglutination potential

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Asian Pacific Journal of Tropical Medicine 2016年第4期9卷 363-370页

作者： Daniel Barroso de Alencar Fátima Cristiane Teles de Carvalho Rosa Helena Rebouas Daniel Rodrigues dos santos Kelma Maria dos santos Pires-Cavalcante rebeca Larangeira de Lima Bárbara Mendes Baracho Rayssa Mendes Bezerra Francisco Arnaldo Viana Regine Helena Silva dos Fernandes Vieira Alexandre Holanda Sampaio Oscarina Viana de Sousa Silvana Saker-Sampaio Laboratrio de Produtos Naturais Marinhos.Departamento de Engenharia de Pesca Universidade Federal do Ceará.Campus do Pici.Ar.Mister Hull.s/nCaixa Postal 6043 Laboratrio de Microbiologia Ambiental e do Pescado.Instituto de Ciências do Mar.Universidade Federal do Ceará.Av.da Abolicao 3207 Laboratrio de Microbiologia Ambiental e do Pescado.Instituto de Ciências do Mar.Universidade Federal do Ceará Laboratrio de Produtos Naturais Marinhos.Departamento de Engenharia de Pesca Universidade Federal do Ceará Laboratrio de Cromatografia Departamento de QuimicaUniversidade do Estado do Rio Grande do NorteCampus Universitário Central

Objective:To evaluate the antioxidant,antibacterial and bacterial cell agglutination activities of the hexane(Hex) and 70%ethanol(70%EtOH) extracts of two species of red seaweeds Pterocladiella capillacea(***) and Osmundaria ***:In vitro antioxidant activity was determined by DPPH radical scavenging assay,ferric-reducing antioxidant power assay,ferrous ion chelating assay,β-carotene bleaching assay and total phenolic content *** activity was tested using the method of disc diffusion on Mueller-Hinton *** ability of algal extracts to agglutinate bacterial cells was also ***:The 70%EtOH extract of the two algae showed the highest values of total phenolic content compared to the Hex *** results of DPPH for both extracts(Hex,70%EtOH) of Osmundaria obtusiloba(43.46%and 99.47%) were higher than those of ***(33.04%and 40.81%) at a concentration of 1 000 μg/*** for the ferrous ion chelating,there was an opposite behavior,extracts of *** had a higher *** extracts showed a low ferric-reducing antioxidant power,with optical density ranging from0.054 to *** activities of all extracts evaluated for β-carotene bleaching were above 40%.There was no antibacterial activity against bacterial strains ***,the extracts of both species were able to agglutinate bacterial Gram positive cells of Staphylococcus aureus and Gram negative cells of Escherichia coli,multidrug-resistant Salmonella and Vibrio *** This is the first report of the interaction between these algal extracts,rich in natural compounds with antioxidant potential,and Gram positive and Gram negative bacterial cells.

关键词： Bioactive compounds Antioxidant Bacterial agglutination Rhodophyta

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