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Bioactive extracts of red seaweeds Pterocladiella capillacea and Osmundaria obtusiloba(Floridophyceae:Rhodophyta) with antioxidant and bacterial agglutination potential

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Asian Pacific Journal of Tropical Medicine 2016年第4期9卷 363-370页

作者： Daniel Barroso de Alencar Fátima Cristiane Teles de Carvalho Rosa Helena Rebouas Daniel Rodrigues dos Santos Kelma Maria dos Santos Pires-Cavalcante rebeca Larangeira de Lima Bárbara Mendes Baracho Rayssa Mendes Bezerra Francisco Arnaldo Viana Regine Helena Silva dos Fernandes Vieira Alexandre Holanda Sampaio Oscarina Viana de Sousa Silvana Saker-Sampaio Laboratrio de Produtos Naturais Marinhos.Departamento de Engenharia de Pesca Universidade Federal do Ceará.Campus do Pici.Ar.Mister Hull.s/nCaixa Postal 6043 Laboratrio de Microbiologia Ambiental e do Pescado.Instituto de Ciências do Mar.Universidade Federal do Ceará.Av.da Abolicao 3207 Laboratrio de Microbiologia Ambiental e do Pescado.Instituto de Ciências do Mar.Universidade Federal do Ceará Laboratrio de Produtos Naturais Marinhos.Departamento de Engenharia de Pesca Universidade Federal do Ceará Laboratrio de Cromatografia Departamento de QuimicaUniversidade do Estado do Rio Grande do NorteCampus Universitário Central

Objective:To evaluate the antioxidant,antibacterial and bacterial cell agglutination activities of the hexane(Hex) and 70%ethanol(70%EtOH) extracts of two species of red seaweeds Pterocladiella capillacea(***) and Osmundaria ***:In vitro antioxidant activity was determined by DPPH radical scavenging assay,ferric-reducing antioxidant power assay,ferrous ion chelating assay,β-carotene bleaching assay and total phenolic content *** activity was tested using the method of disc diffusion on Mueller-Hinton *** ability of algal extracts to agglutinate bacterial cells was also ***:The 70%EtOH extract of the two algae showed the highest values of total phenolic content compared to the Hex *** results of DPPH for both extracts(Hex,70%EtOH) of Osmundaria obtusiloba(43.46%and 99.47%) were higher than those of ***(33.04%and 40.81%) at a concentration of 1 000 μg/*** for the ferrous ion chelating,there was an opposite behavior,extracts of *** had a higher *** extracts showed a low ferric-reducing antioxidant power,with optical density ranging from0.054 to *** activities of all extracts evaluated for β-carotene bleaching were above 40%.There was no antibacterial activity against bacterial strains ***,the extracts of both species were able to agglutinate bacterial Gram positive cells of Staphylococcus aureus and Gram negative cells of Escherichia coli,multidrug-resistant Salmonella and Vibrio *** This is the first report of the interaction between these algal extracts,rich in natural compounds with antioxidant potential,and Gram positive and Gram negative bacterial cells.

关键词： Bioactive compounds Antioxidant Bacterial agglutination Rhodophyta

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Resistive Cerebral Blood Flow as a Potential Marker of Subclinical Brain Damage in Essential Hypertension

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World Journal of Cardiovascular Diseases 2014年第4期4卷 169-178页

作者： Sergio gonzalez-Garcia Zenaida Hernandez-Diaz Luis Quevedo-Sotolongo Marisol Pena-Sanchez Yaima Pino-Pena rebeca Fernandez-Carriera Caridad Menendez-Sainz Alina gonzalez-Quevedo Janis Eells Institute of Neurology and Neurosurgery La HabanaCuba Cira Garcia Central Clinic La HabanaCuba College of Health Sciences University of Wisconsin-MilwaukeeMilwaukeeUSA

Introduction: Subclinical brain damage in essential hypertension is more prevalent than cardiovascular or renal impairment;nevertheless, screening for nervous system involvement is difficult due to the low accessibility and high costs of these techniques. Objective: To assess the frequency of silent target organ damage in a cohort of asymptomatic hypertensive patients and to evaluate the potential usefulness of carotid ultrasonographic (US) variables as predictors of subclinical brain damage. Patients and Methods: Thirty four neurologically asymptomatic subjects (mean age 59 years) with essential hypertension were included. Target organ damage was evaluated: degree of hypertensive retinopathy, heart, kidney and brain. Structural and hemodynamical carotid Doppler US parameters were also investigated. Results: The brain was the most frequently affected target organ (70.6%), followed by the heart (67.9%) and kidney (58.6%). Carotid US parameters showed no association of intima media thickness with brain MRI results;nevertheless, decreased diastolic flow velocity and increased resistive index pointed to a resistive carotid flow pattern in patients with classical brain MRI lesions and predicted subclinical lesions with a sensitivity of 70% and 74% and a specificity of 72% and 80% respectively. Conclusions: This study supports previous findings that place the brain as the most frequently affected target organ in essential hypertensive patients and sheds more light on the potential usefulness of carotid structure and hemodynamics as imaging biomarkers of subclinical brain lesions.

关键词： Subclinical Brain Damage Essential Hypertension Carotid Doppler

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Risk Factors Associated with Mother-to-Child Transmission of HIV-1, Spontaneous Abortion and Infant Mortality in HIV-1 Infected Women in Burkina Faso: A Prospective Study

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Journal of Biosciences and Medicines 2018年第10期6卷 12-21页

作者： Serge Theophile Soubeiga Albert Theophane Yonli Tegwindé rebeca Compaore Abdoul Karim Ouattara Birama Diarra Dorcas Obiri-Yeboah Zoenabo Douamba Lassina Traore Wendkuuni Florencia Djigma Jacques Simpore Pietro Annigoni Biomolecular Research Centre (CERBA) Ouagadougou Burkina Faso Laboratory of Molecular Biology and Genetics (LABIOGENE) University Ouaga I Prof. Joseph KI-ZERBO Ouagadougou Burkina Faso Department of Microbiology and Immunology School of Medical Sciences University of Cape Coast Cape Coast Ghana

Background: Despite efforts to fight, HIV/AIDS mother-to-child transmission of HIV-1 (MTCT), as well as abortion and infant mortality, remains a problem in sub-Saharan Africa. Indeed, a low level of CD4 and a high viral load can be associated with these situations. The aim of this study was to determine the risk factors associated with the occurrence of MTCT, spontaneous abortion and infant mortality in HIV-1 infected women in Ouagadougou, Burkina Faso. This was a prospective study conducted from May 2014 to September 2017 and involved 423 HIV-1 infected women followed at Saint Camille Hospital in Ouagadougou, Burkina Faso. Sociodemographic data were collected through a questionnaire. The CD4 count and HIV-1 viral load were determined using respectively BD FACSCount and Abbott m2000rt instruments. Bivariate analysis and multinomial logistic regression were performed for associations with a significance threshold for p Results: The average age of women was 38.75 ± 7.98 years. Rates of MTCT, abortion and infant mortality were 16.31%, 30.49% and 34.75%, respectively. The number of pregnancies was associated with the number of infant deaths (p = 0.002). A correlation between the number of pregnancies and infant mortality was observed (p = 0.002) with a relatively high rate (28.6%) among women who had three pregnancies. In addition, marital status was associated with HIV-1 infection in infants (p = 0.042) and spontaneous abortion (p = 0.033). HIV-1 infected women with low CD4 counts (less than 350 cells/μL) and those with viral load more than 1000 copies/mL were about twice as likely to have an spontaneous abortion [OR (IC 95%): 2.50 (1.085 - 5.760);p = 0.03] and [OR (95% CI): 2.16 (1.043 - 4.505);p = 0.04]. Conclusions: The results of this study show the need to improve the treatment of HIV-1 infected women in order to restore CD4 levels and make viral load of HIV-1 undetectable.

关键词： HIV-1 MTCT Abortion Infants Burkina Faso

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Meningitis Outbreak Caused by Neisseria meningitidis Serogroup C ST 10217 in 2019 in Diapaga, Burkina Faso

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Advances in Infectious Diseases 2023年第1期13卷 66-76页

作者： Kambiré Dinanibè Tondé Issa Ouédraogo W. H. Gautier Sanou Mahamoudou Ouédraogo L. S. L. Wilfrid Savadogo Mahamoudou Tamboura Mamadou Zongo Moumouni Ouédraogo Oumarou Zouré Abdou Azaque Compaoré rebeca Sagna Tani Zida Sylvie Soubeiga R. S. Théophile Aké Flavien Sangaré Lassana Méda Isaïe Kouanda Séni Ouédraogo-Traoré Rasmata Centre Hospitalier Pédiatrique Universitaire Charles De Gaulle Ouagadougou Burkina Faso Institut de Recherche en Sciences de la Santé Ouagadougou Burkina Faso Direction de la protection de la Santé de la Population Ouagadougou Burkina Faso Centre Hospitalier Universitaire Yalgado Ouédraogo Ouagadougou Burkina Faso Davycas International Ouagadougou Burkina Faso Direction Générale de la Santé Publique Ouagadougou Burkina Faso

Introduction: Meningitis caused by Neisseria meningitidis constitutes a burden for the countries in the meningitis belt of sub-Saharan in general and particularly for Burkina Faso. In 2019 the Diapaga health district experienced a meningitis epidemic due to N. meningitidis serogroup C. Methods: This is a cross-sectional study with a descriptive aim in the health district of Diapaga where all cases of meningitis were included in this work. Rapid diagnostic tests (RDTs), culture as well as real-time PCR were used for the biological analysis of cerebral spinal fluid (CSF) samples. Results: Of 155 CSF samples analysed, 42% (65/155) were tested positve. Of them, N. meningitidis C accounted for 83% of all positive cases. Likewise, all thirteen (13) NmC strains were susceptible to oxacillin, ceftriaxone, penicillin and chloramphenicol. All strains of NmC belonged to the sequence type (ST) 10 217 and to the clonal complex (CC) 10 217. These CCs belonged to the same variant PorA type: P1.21-15.16;FetA type: F1-7;PorB type: 3-463. Conclusion: Burkina Faso had known an epidemic of meningitis caused by NmC in 2019 in the health district of Diapaga. This outbreak was contained in time due to the performance of the epidemiological surveillance system which made it possible to investigate on time and introduce the vaccine against the pathogen NmC.

关键词： Meningitis Neisseria meningitidis VS Diapaga Burkina Faso

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Total hepatectomy and liver transplantation as a two-stage procedure for fulminant hepatic failure: A safe procedure in exceptional circumstances

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World Journal of Hepatology 2016年第4期8卷 226-230页

作者： rebeca Sanabria Mateos Niamh M Hogan Dimitri Dorcaratto Helen Heneghan Venkatesh Udupa Donal Maguire Justin Geoghegan Emir Hoti Hepatobiliary and Liver Transplant Surgical Unit St. Vincent’s University Hospital

AIM: To evaluate the outcomes of two-stage liver transplant at a single institution, between 1993 and March ***: We reviewed our institutional experience with emergency hepatectomy followed by transplantation for fulminant liver failure over a twenty-year period. A retrospective review of a prospectively maintained liver transplant database was undertaken at a national liver transplant centre. Demographic data, clinical presentation, preoperative investigations, cardiocirculatory parameters, operative and postoperative data were ***: In the study period, six two-stage liver transplants were undertaken. Indications for transplantation included acute paracetamol poisoning(n = 3), fulminant hepatitis A(n = 1), trauma(n = 1) and exertional heat stroke(n = 1). Anhepatic time ranged from 330 to 2640 min. All patients demonstrated systemic inflammatory response syndrome in the first post-operative week and the incidence of sepsis was high at 50%. There was one mortality, secondary to cardiac arrest 12 h following re-perfusion. Two patients required re-transplantation secondary to arterial thrombosis. At a median follow-up of 112 mo, 5 of 6 patients are alive and without evidence of graft ***: Two-stage liver transplantation represents a safe and potentially life-saving treatment for carefully selected exceptional cases of fulminant hepatic failure.

关键词： Two-stage liver transplantation Fulminanthepatic failure Liver transplant Survival

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Live and Inactivated Salmonella Enteritidis Vaccines:Immune Mechanisms in Broiler Breeders

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World Journal of Vaccines 2015年第4期5卷 155-164页

作者： Letícia Dal Bérto Breno C. B. Beirao Tobias Fernandes Filho Max Ingberman Celso Favaro Jr. Rosangela Tavella rebeca Buest de Mesquita Silva Luiz Felipe Caron Campus UP Universidade PositivoCuritibaBrazil Imunova Análises Biologicas CuritibaBrazil Departamento de Patologia Basica Universidade Federal do ParanaCuritibaBrazil

Salmonella is a ubiquitous pathogen which, in addition to causing poultry diseases, has a growing zoonotic impact. It has demanded the implementation of diverse control strategies, in which vaccines play a major role. The understanding of the immune pathways elicited by the different vaccines is important, contributing for the establishment of strong immune correlates of protection, for instance. With the purpose of determining the dynamics of the humoral and cellular immune responses to vaccination, broiler breeders (Cobb Slow) were immunized with live or inactivated vaccines against Salmonella Enteritidis. Lymphocyte and macrophage subsets were analyzed in the peripheral blood by flow cytometry and antigen-specific circulating IgY and mucosal IgA were quantified. The markers analyzed by flow cytometry were CD8/CD28, CD4/TCRVβ1, Kul/ MHC II and Bu-1. Both live and inactivated vaccines induced an increase in the proportion of circulating monocytes (Kul+MHCII+) in some time points compared to non-vaccinated controls. However, whereas the live vaccine leads to an increase in CD8-CD28+ and Bu-1+ lymphocytescompared to the control group, the inactivated vaccine prompteda reduction in the percentage of severalleucocyte subsets (Kul-MHCII+, Bu-1+, CD8+CD28+, CD8-CD28+, CD4+TCRVβ1-, CD4+TCRVβ1+, CD4-TCRVβ1+) after the boost dose. Both vaccines induced specific serum IgY and mucosal IgA production;however, the inactivated vaccine stimulated higher titers in a shorter period. These results contribute to the understanding of mechanisms of action of live and inactivated Salmonella vaccines in chickens.

关键词： Salmonella Enteritidis Humoral and Cellular Immunity Live Vaccine Bacterin Broiler Breeders

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南瓜属、种的基因目录,2004

南瓜属、种的基因目录,2004

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中国园艺学会南瓜分会西南地区南瓜产业化发展研讨会暨第三...

作者： Harry S.Paris rebeca Nelson Brown 林德佩四川省农业科学院园艺研究所

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Effects of Biodegradable Detergents in Morphological Parameters of Liver in Two Neotropical Fish Species(Prochilodus lineatus and Astyanax altiparanae)

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Microscopy Research 2014年第2期2卷 39-49页

作者： Bruno Fiorelini Pereira rebeca Mamede da Silva Alves Anderson Luis Alves Jose Augusto Senhorini Rita de Cassia Gimenes de Alcantara Rocha Priscilla Hakime Scalize Dimitrius Leonardo Pitol Flavio Henrique Caetano Universidade Estadual Paulista“Julio de Mesquita Filho”-UNESP Campus de Rio Claro Sao PauloBrazil EMBRAPA PalmasBrazil ICMBio/CEPTA PirassunungaBrazil Faculdade de Odontologia de Ribeirao Preto FORP/USPRibeirao PretoBrazil

Indiscriminate disposal of residues of domestic and/or industrial origin in aquatic ecosystems resulting from urban activities causes concern about their effects on the fauna. Substances absorbed by animals, such as those cited above, are metabolized by the liver, as it is the main target organ of several pollutants and also exercises basic vital functions in the organism. For this reason the liver is used as the main and most reliable biomarker in environmental studies. Thus the objective of the present study was to identify hepatic alterations resulting from exposure to water contaminated by a few commercial brands of biodegradable detergents and their effects when present together with several other contaminants found in nature. For such, a solution of 1 ppm of biodegradable detergents and water from an urban lake were tested on two widely distributed native Brazilian fish (Astyanax altiparanae and Prochilodus lineatus). Several alterations were found on the liver, including cytoplasm vacuolization, nuclear displacement, macrophage recruitment and reduced liver glycogen, showing a degenerative process in the liver of the fish, due to the action of contaminants.

关键词： Liver Fish Detergent Urban Lake Pollution

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中医药在巴拿马的发展现状与分析

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国际中医中药杂志 2021年第5期43卷 435-439页

作者：李皓月石晗 Ana rebeca Almanza 魏竞竞杨卫彬赵静许家杰曹洪欣中国中医科学院中医临床基础医学研究所2009年行业专项办公室北京100700 中国中医科学院国际合作处北京100700 中国中医科学院西苑医院北京100091 对外经济贸易大学国际学院北京100029 北京中医药大学 100029 中国中医科学院研究生院北京100700 美国洛杉矶加州大学东西医学中心 90024 中华中医药学会北京100029

巴拿马国内医疗保险普及工作已基本完成,医保覆盖率较高,但呈两极分化。导致该国成人死亡的主要疾病为缺血性心脏病、脑卒中、AD及糖尿病,近年来慢性肾脏疾病及COPD的发病率亦显著升高。巴拿马本土医学被认为是补充替代医学,已得到国内... 详细信息

巴拿马国内医疗保险普及工作已基本完成,医保覆盖率较高,但呈两极分化。导致该国成人死亡的主要疾病为缺血性心脏病、脑卒中、AD及糖尿病,近年来慢性肾脏疾病及COPD的发病率亦显著升高。巴拿马本土医学被认为是补充替代医学,已得到国内法律认可,在土著人群和农村地区使用率较高。中医药在巴拿马尚处起步阶段,无相关立法,但该国重视本土传统医学且民众接受度逐渐提高,中医药在当地发展具有巨大潜力。建议以文化为载体推进中医药传播、探索多样的中医药合作与交流形式、以传统草药研发为切入点并加大针灸推广力度,推动中医药在巴拿马的发展。

关键词：国际合作巴拿马中医药医学,传统发展现状

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Transmission of severe acute respiratory syndrome coronavirus 2 via fecal-oral:Current knowledge

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World Journal of Clinical Cases 2021年第28期9卷 8280-8294页

作者： Filipe Antônio França da Silva Breno Bittencourt de Brito Maria Luísa Cordeiro Santos Hanna Santos Marques Ronaldo Teixeira da Silva Júnior Lorena Sousa de Carvalho Samuel de Sousa Cruz Gabriel Reis Rocha Gabriel Lima Correa Santos Kathlen Coutinho de Souza rebeca Gabrielle Almeida Maciel Daiana Silva Lopes Natália Oliveira e Silva Márcio Vasconcelos Oliveira Fabrício Freire de Melo Instituto Multidisciplinar em Saúde Universidade Federal da BahiaVitória da Conquista 45002175BahiaBrazil Departamento de Ciências Naturais Universidade Estadual do Sudoeste da BahiaVitória da Conquista 45002175BahiaBrazil Departamento de Bioquímica e Biofísica Universidade Federal da BahiaSalvador 40.110-100BahiaBrazil Instituto Multidisciplinar em Saúde Universidade Federal da BahiaVitória da Conquista 45029-094BahiaBrazil

The pandemic caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)has resulted in more than 93 million cases and 2 million deaths in the ***-CoV-2 respiratory tract infection and its main clinical manifestations such as cough and shortness of breath are well known to the scientific ***,a growing number of studies have reported SARS-CoV-2-related gastrointestinal involvement based on clinical manifestations,such as diarrhea,nausea,vomiting,and abdominal pain as well as on the pathophysiological mechanisms associated with coronavirus disease ***,current evidence suggests SARS-CoV-2 transmission via the fecal-oral route and aerosol ***,studies have shown a high risk of contamination through hospital surfaces and personal ***,viable SARS-CoV-2 specimens can be obtained from aerosols,which raises the possibility of transmission through aerosolized viral particles from ***,the infection by SARS-CoV-2 via fecal-oral route or aerosolized particles should *** addition,a possible viral spread to sources of drinking water,sewage,and rivers as well as the possible risk of viral transmission in shared toilets become a major public health concern,especially in the least developed *** authors have emphasized the presence of viral RNA and even viable SARS-CoV-2 in human feces,studies on the possible fecal-oral coronavirus disease 2019 transmission become essential to understand better the dynamics of its transmission and,then,to reinforce preventive measures against this infection,leading to a more satisfactory control of the incidence of the infection.

关键词： COVID-19 SARS-CoV-2,Transmission Via fecal-oral

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