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Plant Phosphatidylcholine-Hydrolyzing Phospholipases C NPC3 and NPC4 with Roles in Root Development and Brassinolide Signaling in Arabidopsis thaliana

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Molecular Plant 2010年第3期3卷 610-625页

作者： Rinukshi Wimalasekera Pre.ysl Pejchar Andre.Holka Jan Martinec Gunther re. Schere. Leibniz University of Hannover Institute of Floriculture and Wood Science Section of Applied Molecular Physiology Herrenhauser Strasse 2 D-30419Hannover Germany Institute of experimental Botany Academy of Sciences of the Czech Republic v.v.i. Rozvojova 263 165 02 Prague 6 Czech Republic

Phosphatidylcholine-hydrolyzing phospholipase C （PC-PLC） catalyzes the hydrolysis of phosphatidylcholine （PC） to generate phosphocholine and diacylglycerol （DAG）. PC-PLC has a long tradition in animal signal transduction to generate DAG as a second messenger besides the classical phosphatidylinositol splitting phospholipase C （PI-PLC）. Based on amino acid sequence similarity to bacterial PC-PLC, six putative PC-PLC genes （NPC1 to NPC6） were.identified in the Arabidopsis genome. RT-PCR analysis re.ealed overlapping expre.sion pattern of NPC genes in root, stem, leaf, flower, and silique. In auxin-tre.ted PNPc3：GUS and PNPc4：GUS seedlings, strong incre.se of GUS activity was visible in roots, leaves, and shoots and, to a weaker extent, in brassinolide-tre.ted （BL） seedlings. PNPc4：GUS seedlings also re.ponded to cytokinin with incre.sed GUS activity in young leaves. Compare. to wild-type, T-DNA insertional knockouts npc3 and npc4 showed shorter primary roots and lower lateral root density at low BL concentrations but incre.sed lateral root densities in re.ponse to exogenous 0.05-1.0 I^M BL BL-induced expre.sion of TCH4 and LRX2, which are.involved in cell expansion, was impaire. but not impaire. in re.re.sion of CPD, a BL biosynthesis gene, in BL-tre.ted npc3 and npc4. These observations suggest NPC3 and NPC4 are.important in BL-mediated signaling in root growth. When tre.ted with 0.1 I^M BL, DAG accumulation was observed in tobacco BY-2 cell culture. labeled with fluore.cent PC as early as 15 min after application. We hypothesize that at least one PC-PLC is a plant signaling enzyme in BL signal transduction and, as shown earlier, in elicitor signal transduction.

关键词： Auxin brassinolide signaling phosphate deficiency phosphatidylcholine-splitting phospholipase C (PC-PLC) NPC genes.

A New Method of Construction of Robust Second Order Rotatable Designs Using Balanced Incomplete Block Designs

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Open Journal of Statistics 2012年第1期2卷 39-47页

作者： B. re. Victorbabu K. Rajyalakshmi 不详

The following article has been re.racted due to the investigation of complaints re.eived against it. Title: A New Method of Construction of Robust Second Order Rotatable Designs Using Balanced Incomplete Block Designs Authors: Bejjam re. Victorbabu, Kottapalli Rajyalakshmi. The above paper is a copy of Dr. Rabindra Nath Das’s former article, entitled '“Robust second order rotatable designs (Part I)”. The scientific community takes a very strong view on this matter and we solemnly withdrawn?the paper from the journal OJS. This paper published in OJS Vol.2 No.1, 39-47, 2012, has been re.racted.

关键词： re.ponse Surface Designs Rotatability Second Order Rotatable Designs (SORD) Robust SORD Robustness Balanced Incomplete Block Designs

Patterns and Timing in expre.sion of early Auxin-Induced Genes Imply Involvement of Phospholipases A （pPLAs） in the re.ulation of Auxin re.ponses

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Molecular Plant 2013年第5期6卷 1473-1486页

作者： Corinna Labusch Maria Shishova Yunus effendi Maoyin Li Xuemin Wang Gunther re. Schere.a Leibniz Universit&t Hannover Institut for Zierpflanzenbau und Geh61zwissenschaften Abt. Molekulare Ertragsphysiologie Herrenhauser Str. 2 D-30419 Hannover Germany Department of Plant Physiology and Biochemistry State University of St. Petersburg Universitetskaya em. 7/9 St. Petersburg 199034 Russia Department of Biology University of Missouri St Louis MO 63121 USA Donald Danforth Plant Science Center St Louis MO 63132 USA

While it is known that patatin-re.ated phospholipase A （pPLA） activity is rapidly activated within 3min by auxin, hardly anything is known about how this signal influences downstre.m re.ponses like transcription of early auxin-induced genes or other physiological re.ponses. We scre.ned mutants with T-DNA insertions in members of the pPLA gene family for molecular and physiological phenotypes re.ated to auxin. Only one in nine Arabidopsis thaliana ppla knockdown mutants displayed an obvious constitutive auxin-re.ated phenotype. Compare. to wild-type, ppla-IIlδ mutant seedlings had decre.sed main root lengths and incre.sed lateral root numbers. We tested auxin-induced gene expre.sion as a molecular re.dout for primary molecular auxin re.ponses in nine ppla mutants and found delayed up- re.ulation of auxin-re.ponsive gene exRre.sion in all of themL Thirty minutes after auxin tre.tment, up-re.ulation of up to 40% of auxin-induced genes was delayed in mutant seedlings. We observed only a few cases with hypersensitive auxin-induced gene expre.sion in ppla mutants. While, in thre. ppla mutants, which were.investigated in detail, rapid up- re.ulation （as early as 10 min after auxin stimulus） of auxin-re.ulated genes was impaire., late transcriptional re.ponses were.wild-type-like. This re.ulatory or dynamic phenotype was consistently observed in differe.t ppla mutants with delayed up-re.ulation that fre.uently affected the same genes. This defect was not affected by pPLA transcript levels which re.ained constant. This indicates aposttranslational mechanism as a functional link of pPLAs to auxin signaling. The need for a re.eptor triggering an auxin re.ponse without employing transcription re.ulation is discussed.

关键词： hormonal re.ulation hormone biology signal transduction.

Investigation and Analysis on Urban Farm Labore.s’ Childre. Health

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Investigation and Analysis on Urban Farm Laborers’ Children...

INFLUeNCe FACTORS ON DISeASeS AND SUBSeQUeNT DeVeLOPMeNT OF CALVeS

第4届全国青年体育科学学术会议

作者： ZHAI Lin School of re. in CCNU, Wuhan, 430079

＜正＞re.earch Objective:The re.earch aims to thoroughly investigate the urban farmer labore.s’ childre. health and the changes of their health in their adaptation to the urban life, study and mental environment as w...

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INFLUENCE FACTORS ON DISEASES AND SUBSEQUENT DEVELOPMENT OF ...

earthquake distribution and lithospheric rheology beneath the Northwestern Andes, Colombia

面向21世纪的动物科学与动物医学国际研讨会

作者： Heide-Drte Matthes Vadim Pastushenko re. Rudolf Werner Jentsch Department of Agricultural Science Rostock University Department of Agricultural Science Rostock University re.earch Institute for Biology of Farm Animals re.earch Institute for the Biology of Farm animals

＜正＞Aim of the investigations was to analyze the re.sons and influence factors on the diseases of male calves from two big re.ring farms. 2507 calves, descending from 87 differe.t bulls（farm No 1） and 23954 calves, descending from 366 bulls（farm No 2） were.involved. The animals were.put in the farm with 24±8re.pectively 45±17days of age, weighing 51±11re.p. 58±11kg. The calves were.tied in calf stalls of 800-1200 places, exactly numbere. each place.

关键词： Diarrhea Pneumonia

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Geodesy and Geodynamics 2021年第1期12卷 1-10页

作者： Carla Lagardère.Carlos A.Vargas Department of Geosciences Universidad Nacional de ColombiaBogotaColombia ecole nationale superieure.en environnement georessources et ingenierie du developpement durable(ENSEGID)BordeauxFrance

The rheological behavior of the lithosphere.beneath the Northwestern(NW)Andes(Colombian)re.ains uncertain under a complex geological ***,we analyze the earthquake distribution and the stre.s drop on the western and the eastern of the transition are. between the Upper Magdalena Valley(UMV)and the Middle Magdalena Valley(MMV),a zone re.ated to a plate tectonic transition from steeper to flatter subduction style slabs(Nazca and Caribbean plates).Our re.ults consistently explain the tectonic and geodynamic context of the study are. supported in two rheological profiles,which re.eal the brittle/ductile transition of the lithospheric crust and mantle and allow to constrain the lithospheric rheological *** subduction phenomenon of the Nazca Plate under the South American Plate is observed in the western *** the eastern re.ion,we find a re.arkable aseismic zone under the eastern Cordillera below 20-km-deep,which made more.difficultan accurate estimation of the crust/mantle *** seismic particularity leads us to support the hypothesis of a delamination process due to the tectonic overload generated by the eastern Cordillera,and that promotes thermal *** re.ults also suggest that the earthquake dataset corre.ated with rheological estimations may offer a consistent interpre.ation of the mechanical behavior of the lithosphere.

关键词： Lithosphere.rheology Seismicity Northwestern Andes Delamination process

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卫星网与地面蜂窝区网综合的移动通信

电信快报 1992年第7期 1-6页

作者： re.,eD 王绍徐

A unified analysis of a micro-beam,droplet and CNT ring adhere. on a substrate:Calculation of variation with movable boundaries

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Acta Mechanica Sinica 2013年第1期29卷 62-72页

作者： Jian-Lin Liu re.Xia School of Pipeline and Civil engineering China University of Petroleum School of Power and Mechanical engineering Wuhan University

In this study, we developed a general method to analytically tackle a kind of movable boundary problem from the viewpoint of energy variation. Having grouped the adhesion of a micro-beam, droplet and carbon nanotube （CNT） ring on a substrate into one framework, we used the developed line of re.soning to investigate the adhesion behaviors of these systems. Based upon the derived governing equations and transversality conditions, explicit solutions involving the critical parameters and morphologies for the thre. systems are.successfully obtained, and then the parameter analogies and common characteristics of them are.thor- oughly investigated. The pre.ented method has been verified via the concept of energy re.ease rate in fracture.mechanics. Our analyses provide a new approach for exploring the mechanism of differe.t systems with similarities as well as for understanding the unity of nature. The analysis re.ults may be beneficial for the design of nano-structure. materi- als, and hold potential for enhancing their mechanical, chemical, optical and electronic properties.

关键词： Variational theory - Transversality condition Beam adhesion Droplet adhesion Carbon nanotube ring adhesion

THe STATeMeNT ON THe COMMeRCIAL SPORTS

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THE STATEMENT ON THE COMMERCIAL SPORTS