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Pharmacological studies on Buchanania lanzan Spreng.-A focus on wound healing with particular reference to anti-biofilm properties

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Asian Pacific Journal of Tropical Biomedicine 2013年第12期3卷 967-974页

作者： Ashok Pattnaik ratul Sarkar Amrita Sharma Kirendra Kumar Yadav Alekh Kumar Paramita Roy Avijit Mazumder Sanmoy Karmakar Tuhinadri Sen Department of Pharmaceutical Sciences Birla Institute of Technology Division of Pharmacology Department of Pharmaceutical TechnologyJadavpur University School of Natural Product Studies Department of Pharmaceutical TechnologyJadavpur University Department of Pharmaceutical Technology Noida Institute of Engineering and Technology

Objective:To evaluate the wound healing activity of the methanolic root extract of Buchanania lanzan Spreng.(***),with a focus on antimicrobial and anti-biofilm ***:The extract was evaluated for its wound healing properties(excision and incision models)as evident from the analysis of tensile strength and wound *** extract was also screened for antibacterial properties against different Cram positive and Gram negative bacterial *** was also studied for its effect on biofilm formation and disruption of preformed *** synergistic effect of *** was determined in combination with ***:Topical application of ***(10%w/w ointment)significantly increased(40.84%)the tensile strength in the incision wound *** also showed significant wound healing activity in excision model and such significant activity was observed from the 9th *** Soframycin displayed significant wound healing activity from the 6th *** was found that root extracts of *** revealed significant inhibition against all tested *** displayed antimicrobial activity against Cram positive(MIC 0.625 mg/mL)and Cram negative(MIC0.625-1.25 mg/mL).*** was able to reduce biofilm formation and also caused disruption of preformed biofilms in a manner similar to ***,gentamicin was found to be ineffective against biofilms formed by Gram negative *** to the fractional inhibitory concentration index,*** displayed synergistic activity when it was combined with ***:From this study it may be concluded that the root extract of *** revealed significant wound healing potential,which was supported and well correlated with pronounced antibacterial activity of the tested plant parts.

关键词： Buchanania lanzan Root and leaf extracts Wound healing Incision Excision Antibacterial Antibiofilm

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Regeneration ecology and population status of a critically endangered and endemic tree species (Ilex khasiana Purk.) in north-eastern India

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Journal of Forestry Research 2009年第3期20卷 223-228,286页

作者： Upadhaya Krishna Barik Saroj Kanta Adhikari Dibyendu Baishya ratul Lakadong Nigyal John Division of Environmental Science Department of Basic Sciences and Social Sciences School of Technology North-Eastern Hill University Centre for Advanced Studies in Botany School of Life Sciences North-Eastern Hill University

Detailed studies on population structure and regeneration ecology of Ilex khasiana were carried out at five representative natural populations i.e. Mylliem (Population–I), Upper Shillong (Population–II), Shillong Peak (Population–III), Laitkor (Population–IV) and Nongpiyur (Population–V) to probe into the responsible affecting factors for its population structure and regeneration ability in these populations. Experimental observation under three controlled light conditions (full sunlight (100%), intermediate sunlight (50%) and low sunlight (30%)) showed that the growth status and survival rates of seedlings under intermediate and low light were better than those under the high light condition. The density-diameter distribution in population of adult trees (≥5cm dbh) exhibited a typical character of a regenerating population. A higher seedling mortality rate in natural populations of I. khasiana was most probably due to increase in light intensity following vegetation destruction and other anthropogenic disturbances that cause opening of forest canopy. The findings of the present study would be of immense value in formulating appropriate conservation measures for the species.

关键词： Ilex khasiana endangered species population status regeneration north-eastern India

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The Synthesis of Gold Nanoparticles Using <i>Amaranthus spinosus</i>Leaf Extract and Study of Their Optical Properties

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Advances in Materials Physics and Chemistry 2012年第4期2卷 275-281页

作者： ratul Kumar Das Nayanmoni Gogoi Punuri Jayasekhar Babu Pragya Sharma Chandan Mahanta Utpal Bora Bioengineering Research Laboratory Department of Biotechnology Indian Institute of Technology Guwahati Guwahati India Biotech Hub Centre for the Environment Indian Institute of Technology Guwahati Guwahati India Department of Civil Engineering Indian Institute of Technology Guwahati Guwahati India

We report here the application of a medicinally important plant Amaranthus spinosus for the synthesis of gold nanoparticles (AuNPs). Different concentrations of ethanolic leaf extract of the plant were reacted with aqueous solution of HAuCl4·4H2O under mild reaction conditions. Synthesis of AuNPs was confirmed from the UV-Vis study of surface plasmon resonance property of the colloidal solution. Transmission electron microscopy (TEM) revealed particles as spherical and triangular in shape. X-ray diffraction (XRD) confirmed the crystalline nature of AuNPs with average size of 10.74 nm as determined by Debye-Scherrer’s Equation. Fourier transform infra-red (FT-IR) analysis of leaf extract and lyophilized AuNPs showed the presence of various functional groups present in diverse phytochemicals. Energy dispersive X-ray (EDX) of purified AuNPs confirmed the formation of AuNPs and surface adsorption of biomolecules. We further investigated the toxicity of the synthesized AuNPs and found non toxic to the cancer cell lines and could be used for biomedical applications.

关键词： Gold Nanoparticles Leaf Extract Anti-Oxidant Amaranthus spinosus Crystal Growth

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