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Influence of thiol stress on oxidative phosphorylation and generation of ROS in Streptomyces coelicolor

Journal of Biophysical Chemistry 2010年第3期1卷 172-176页

作者： Hemendra J. Vekaria ratna prabha Chivukula 不详

Thiols play very important role in the intracellular redox homeostasis. Imbalance in the redox status leads to changes in the intracellular metabolism including respiration. Thiol stress, a reductive type of stress can also cause redox imbalance. When Gram-positive bacterium Strep- tomyces coelicolor was exposed to thiol stress, catalaseA was induced. Induction of catalaseA is the consequence of elevation of ROS (reactive oxygen species). The two major sources of reactive oxygen species are Fenton reaction and slippage of electrons from electron transport chain during respiration. Hence, the effect of thiol stress was checked on the rate of oxidative phosphorylation in S. coelicolor. We found correlation in the increase of oxidative phosphorylation rate and the generation of ROS, subsequently leading to induction of catalase. It was observed that thiol stress does not affect the functionality of the individual complexes of the ETC, but still there was an increase in the overall respiration, which may lead to generation of more ROS leading to induction of catalase.

关键词： Streptomyces coelicolor DTT Oxidative Phosphorylation CatalaseA ROS

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Induced Accumulation of Polyphenolics and Flavonoids in Cyanobacteria under Salt Stress Protects Organisms through Enhanced Antioxidant Activity

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American Journal of Plant Sciences 2014年第5期5卷 726-735页

作者： Dhananjaya P. Singh ratna prabha Kamlesh K. Meena Lalan Sharma Arun K. Sharma National Bureau of Agriculturally Important Microorganisms Indian Council of Agricultural Research Kushmaur Maunath Bhanjan 275103 India National Institute of Abiotic Stress Management Indian Council of Agricultural Research Baramati Pune 413115 India

Induced accumulation of polyphenolics and flavonoids in cyanobacterial strains grown under different salt concentrations is correlated with their growth under stress conditions and enhanced antioxidant activity. Plectonema boryanum, Hapalosiphon intricatus, Anabaena doliolum and Oscillatoria acuta grown for 21 days under different salt concentrations (80, 160, 240, 320 and 400 mM) in BG11 medium showed differential growth responses in terms of biomass, total protein, chlorophyll content, total content of polyphenol, flavonoid and carotenoid, accumulation of phenolic acids (gallic, caffeic, chlorogenic, ferullic and vanillic) and flavonoids (rutin and quercetin). Cyanobacterial extracts showed prominent free radical scavenging antioxidant activity (AOA) in terms of % DPPH discoloration. Highly significant (p < 0.05) and strong correlation was found between TPC and AOA (r = 0.974). Other positive but non-significant (p < 0.05) correlations were observed between AOA and gallic acid (r = 0.893) and AOA and caffeic acid (r = 0.931). Significant and strong correlation was also observed between gallic and caffeic acid (r = 0.973). Positive but lesser magnitude correlations were recorded between TPC and caffeic acid (r = 0.905), TPC and gallic acid (r = 0.920), gallic and vanillic acid (r = 0.916) and caffeic and vanillic acid (r = 0.814). An integrated combination of growth parameters, salt-induced accumulation of phenylpropanoids and stress-derived subsequent antioxidant property of cyanobacterial extracts is thought to provide evidence that secondary metabolic changes can act as the possible alternative mechanism to overcome stress-induced damages in cells.

关键词： Cyanobacteria Phenylpropanoids Flavonoids Antioxidants Stress Tolerance

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