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Correlation between Wnt Signalling Inhibitors (Dickkopf-1 & Sclerostin) and the Intimal Medial Thickness in Children on Maintenance Hemodialysis

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Open Journal of Nephrology 2024年第3期14卷 397-412页

作者： Manal abd el-Salam Mohamed Hamdi Ali Nadia Youssef Riad rasha.abd el samad fawaz Shima Mohamed Department of Pediatrics Faculty of Medicine (for Girls) Al-Azhar University Cairo Egypt Tbilisi State Medical University Tbilisi Georgia Department of Clinical Pathology National Heart Institute Cairo Egypt Department of Radiology Faculty of Medicine (for Girls) Al-Azhar University Cairo Egypt

Background: Wnt signalling inhibitors (Dickkopf-1 and Sclerostin) signalling play a role in vascular development and may contribute to calcification. Aim: To investigate the association between Dickkopf-1 and sclerostin serum concentrations in children undergoing maintenance hemodialysis with intimal medial thickness and peak systolic velocity of the main arteries. Patients and Methods: A study was conducted on 40 children undergoing maintenance hemodialysis and controls of the same age and sex. The study measured the initial medial thickness (IMT) and peak systolic velocity (PSV) of the main vessels (carotid, ulnar, and femoral). Dickkopf-1 and sclerostin serum levels in both groups were assessed, and a routine investigation was performed. Results: The findings indicate that the levels of serum Dickkopf-1 and Sclerostin were significantly higher in the hemodialysis group 2540.65 (2215.4 - 2909.2 pg/ml) and 1.17 (0.85 - 2.03 ng/ml)respectively (P = 0.001), compared to their control group it was 1110.45 (885.45 - 1527.65 pg/ml) and 0.28 (0.25 - 0.32 ng/ml)) respectively P = 0.001. Additionally, there was a significant increase in intima-media thickness (IMT) with a decrease in peak systolic velocity (PSV) in the main blood vessels, including the carotid, ulnar, and femoral arteries. A significant correlation was also observed between Dickkopf-1 and sclerostin levels and IMT of the carotid, ulnar, and femoral arteries. Conclusion: Wnt signalling inhibitors (Dickkopf-1 and Sclerostin) exert effects beyond the bone and significantly contribute to early vascular calcification in pediatric patients undergoing maintenance hemodialysis.

关键词： Wnt Signalling Vascular Calcification Hemodialysis Children

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Physio-biochemical and nutrient constituents of peanut plants under bentazone herbicide for broad-leaved weed control and water regimes in dry land areas

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Journal of Arid Land 2020年第4期12卷 630-639页

作者： Hani S SAUDY Ibrahim M el–METWALLY Goma A abd el–samad Agronomy Department Faculty of AgricultureAin Shams University68–Hadayek Shoubra 11241CairoEgypt Botany Departments National Research CentreEl–Behos St.Dokki 12622CairoEgypt

The abundance of broad-leaved weeds in peanut fields represents the handicap in weed management programs, since limited specific herbicides can be recommended to control them. Moreover, the physio-biochemical constituents and nutritional status in peanut plants as affected by available herbicides, i.e., bentazone under water stress conditions are not well known. Therefore, field trials were conducted during the growing seasons in 2016 and 2017 to investigate the interactional impact of irrigation levels(I_(50), I_(75) and I_(100), representing irrigation by 50%, 75% and 100% of crop evapotranspiration, respectively) and weed control practices(bentazone, bentazone+hoeing once, hoeing twice and weedy check as control) on dominant broad-leaved weeds as well as peanut physiological and agronomic traits. Result indicated that the efficiency of weed control for each weeded treatment under I_(50) significantly equaled with its counterpart under I_(75) or I_(100). Bentazone+hoeing once diminished weed biomass by 89.3% and enhanced chlorophyll content of peanut plants by 51.2%. Bentazone relatively caused a reduction in carotenoides. Hoeing twice and bentazone+hoeing once under I_(100) in both growing seasons as well as hoeing twice under I_(75) in 2017 were the superior combinations for boosting pod yield of peanut plants. Treatment of bentazone+hoeing once and I_(75) recorded the lowest reduction in N utilization percentage and the highest increase in potassium utilization percentage of peanut plants. eliminating weeds enhanced water use efficiency by 37.8%, 49.6% and 34.7% under I_(50), I_(75) and I_(100), respectively. In conclusion, peanut seems to be tolerant to bentazone at moderate water supply, thus it can be safely used in controlling the associated broad-leaved weeds.

关键词： biochemical traits drought nutritional status peanut productivity weed growth

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Surgical Management of Mitral Valve Infective Endocarditis with Accidental Finding of Open Pulmonary Tuberculosis

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World Journal of Cardiovascular Surgery 2024年第5期14卷 61-67页

作者： Ahmad Al Khaddour Mohammed Alharthi Alawab M. Mesallam Mohamed Agawi Mahmoud Ghalwash Ahmed Gotbi Mohamed abdel Hafez Fouly Marko Samanovic Nelson David Vega Murad el Gharbi fawaz Altaf King abdullah Medical Complex Jeddah KSA

Objective: This case report aimed to highlight intersections of TB and Cardiovasular diseases which carry high morbidity and mortality rates. Methods: We are reporting the surgical management of forty seven years female who had back ground history of IDDM (Insulin dependent diabetic Mellitus), ESRD (End stage renal disease) on HD (haemodialysis) also she had left subclavian artery stenosis, and paroxysmal atrial fibrillation. She was diagnosed with mitral valve infective endocarditis and found accidently to have an open pulmonary tuberculosis (TB) on the day before surgery. Results: She was started on first line anti-TB treatment. She was isolated in her private room and airborne precautions measures applied. The patient underwent a tissue Mitral Valve replacement and tricuspid valve repair annuloplasty. Special precautions were applied in Theatre and on cardiopulmonary bypass Machine guided by KAMC-J disinfection protocol. The patient made good recovery postoperatively. She was discharged well on day 7 post operatively. Conclusion: Intersections of TB and cardiovasular diseases carry high morbidity and mortality rates. Early diagnosis and early anti tuberculosis treatment can surely improve the patient prognosis. Our decontamination and disinfective procedures are recommended. Cases like this should be monitored long term for the development of further cardiovascular complication.

关键词： Tuberculosis (TB) Mycobacterium tuberculosis (MTB) Heater-Cooler and Bypass Machine New York Heart Association (NYHA)

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The Potential Role of Osmotic Pressure to Exogenous Application of Phytohormones on Crop Plants Grown under Different Osmotic Stress

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American Journal of Plant Sciences 2016年第6期7卷 937-948页

作者： Hamdia M. abd el-samad Botany Department Faculty of Science Minia University El-Minia Egypt

The osmotic pressure represented as a sign of plant tolerance or sensitive to salinity stress. In the following plants, the increase in OP seems to be a manner of defense mechanism to survive. OP increased in shoots of maize, shoot and root of wheat and cotton plants was concomitant with shoot soluble sugar, root soluble protein and shoot and root amino acids of maize plants. However, in wheat the increase in OP was related with increase of root soluble sugar and protein of shoots and roots. In cotton plants, the elevation of OP was run parallel with increase soluble sugar of shoots and roots, shoot soluble protein and root amino acids. The increase in OP was related with a marked and significant reduction in the water content of these plants. However, the decrease in OP of shoot and root of broad bean was related with the reduction of shoots and roots soluble sugar, protein and root amino acids of broad bean. While the OP becomes more or less unchanged in shoots and tended to decrease in root of parsley plants, this concomitant with unchanged trend in the shoots amino acids and reduction in root soluble sugar and root amino acids. Run with previous trend values of OP and metabolites of parsley plants were related with stable values in shoot water content and reduction in root water content. With GA3 and kinetin treatments mostly increase the values OP which parallel with increase and soluble sugar, soluble protein and amino acids contents of shoots and roots of maize, wheat, cotton, broad bean and parsley plants with NaCl increasing. This related with increase water uptake by roots in these plants. The results indicated that kinetin had a more effective to shoot maize, both organs of wheat, broad bean and parsley plants in response to salinity stress while GA3 was more effective on cotton plants especially at higher levels of salinity. Thus plants strategy differed in their tolerance to salinity stress according to their species and differed a

关键词： Osmotic Pressure GA3 and Kinetin NaCl

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Assessment of Indoor and Outdoor Radon Levels in South Lebanon

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International Journal of Disaster Risk Science 2014年第3期5卷 214-226页

作者： Mohammed A.Kobeissi Omar el samad Khaled Zahraman Ibrahim Rachidi Lebanese Atomic Energy Commission National Council for Scientific ResearchRiad ElsolhBeirutLebanon Department of Physics Faculty of Sciences(I)The Lebanese UniversityHadethBeirutLebanon

The presence of high indoor radon concentrations,Cx,is a major concern of the public *** of indoor radon in South Lebanon have been achieved using CR-39 *** ranged between 30 and 122 Bq m-3 in one of the major towns in the area and are compared with measured atmospheric air *** seasonal variation of Cxindoors has been observed,although 90%of the obtained indoor Cxmeasurements are below 100 Bq *** homes produced Cxreadings above the permissible *** showed that the ventilation effect played a major factor in reducing radon concentration levels *** assessment has been *** effective doses of radon varied from 1.01±0.10 to 8.65±0.50 m Sv a-1 in six homes in one of the four cases *** dosage values are above the permitted limit of 2.5 m Sv *** types of granite contained in the studied homes were identified and their contributions to radon levels indoors were calculated.

关键词： Building materials CR-39 Effective dose Granite exhalation rate Lebanon Radon risk assessment

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The Biphasic Role of Cupper and Counteraction with <i>Azospirillum brasilense</i>Application on Growth, Metabolities, Osmotic Pressure and Mineral of Wheat Plant

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American Journal of Plant Sciences 2017年第5期8卷 1182-1195页

作者： Hamdia M. abd el-samad Botany and Microbiology Department Faculty of Science Minia University El-Minia Egypt

The interactive effect of different Cu++ concentrations (5 mM, 10 mM, 20 mM and 25 mM) and treatments with biofertilizers Azospirillum brasilense on growth, metabolites, minerals and osmotic pressure of wheat plants was investigated. Shoots and roots of wheat plant were differentially response to Cu++ treatments, while shoot organ response positively to this treatment, root response negatively. The positive effect of Cu++ in shoot organ was concomitant with the increase in the production of fresh, dry matter, length and water content and this related with the accumulation of soluble sugar, soluble protein and mineral as a result of increasing osmotic pressure. On the other side, the negative effect of Cu++ on root organ was concomitant with the decrease in production of fresh, dry matter, length and water content that related with the reduction in the accumulation of soluble sugar and mineral with the insignificant change in osmotic pressure. Azospirillum brasilense inoculation increased the accumulation of soluble sugar and soluble protein which reflected an increase in the production of fresh, dry matter and water content with increasing values of osmotic pressure of the tested plants under Cu++ treatment. Finally, wheat plants response differentially to Cu++ treatment according to its organ and Azospirillum brasilense treatment improved wheat plant efficiency to tolerate the effect of Cu++ stress.

关键词： Cu++ Treatment Azospirillum brasilense Wheat

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Strategy Role of Mycorrhiza Inoculation on Osmotic Pressure, Chemical Constituents and Growth Yield of Maize Plant Gown under Drought Stress

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American Journal of Plant Sciences 2019年第6期10卷 1102-1120页

作者： Hamdia Mahmoud abd el-samad Khloud Nagy Shaker abd el-Hakeem Botany and Microbiology Department Faculty of Science Minia University El-Minia Egypt

The present work was carried out to investigate the role of mycorrhiza inoculation at two harvesting stages (90-days and 30-days) of maize plants grown in pot experiment with different moisture content levels 100%, 70%, 50% and 20%. Drought stress tolerant in maize plant was varied in different organs of the same plants and also varied among different stage of plant development. The sensitivity of maize plants was related with reduction of root soluble sugar, shoot and root soluble protein at 30-days of plant harvesting, and soluble sugar and soluble protein in both organs of both harvesting stages. This related with reduction in OP and lowering of water uptake which induced a marked decrease in fresh and dry matter production in shoot and root of both harvesting stages. AM inoculation increase maize tolerant to drought stress presented in increasing growth parameters, chemical constituents and minerals contents compared with untreated plants. Proline content with AM inoculation was more or less unchanged in shoot of plant harvesting at 30-days and in root of plant harvesting at 90-days. However, a marked increase was induced in plant harvesting at 30-days and in shoot of plant harvesting at 90-days. Mycorrhiza inoculation induced a significant increase in OP value either compared with corresponding level or compared with control value 100% as in plant 30-days of harvesting or compared with control only as in plant harvesting after 90-days. AM infection with different moisture content levels measured by N-acetyl glucosamine content were not affected by drought stress. Results showed also that control roots contained N-acetyl glucosamine would be attributed to mycorrhiza and other fungi naturally present in soil.

关键词： Moisture Content Mycorrhiza Inoculation Maize Plant

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The Impact of Minerals on Wheat Plants Grown under Salinity Stress

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American Journal of Plant Sciences 2022年第4期13卷 541-556页

作者： Rania M. Taha Hamdia Mahmoud abd el-samad Department of Botany Faculty of Science Minia University El-Minia Egypt

The present work has been conducted to study the impact of Zn (20 and 200 μM) or Ni (1 and 100 μM) on growth, metabolic activities, osmotic pressure and heavy metal of wheat cultivar (cv. Gimiza 11) under salt stress conditions. Harvest index was significantly decreased by 75% at -0.3 MPa and -1.2 MPa decreased by 53%. Length of root, stem and spike, and leaf area were slightly reduced up to -0.9 MPa NaCl level, after that a significant reduction was recorded. Total sugar was increased in the shoot and spike, while decreased in the root with increasing salinity levels. On the other side, total protein was increased in the shoot while decreased in both the root and spike. Osmotic pressure was increased as increasing salinity levels. Treatment of wheat plants with Zn or Ni alleviated generally salt injury in the harvest index, length of root, shoot and spike and leaf area. This effect was more obvious with Zn treatments. Mineralization treatments increased total sugar in the three tested organs while did not change the total protein. Zn content was increased at -1.2 MPa level while Ni was decreased. Treatments with Zn or Ni mostly increased Zn while causing a reduction in the Ni content. These treatments also increased the values of osmotic pressure compared with the equivalent levels of control plants.

关键词： Osmotic Stress Wheat Zn Ni Tolerant Sensitive

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Growth, Metabolites, Protein Profile and Esterase Enzyme of Wheat Grown under Osmotic Stress with Exogenous Application of <i>Allium sativum</i>

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American Journal of Plant Sciences 2018年第4期9卷 902-919页

作者： Hamdia M. abd el-samad D. Mostafa Department of Botany and Microbiology Faculty of Science Minia University El-Minia Egypt

The present work was conducted to lower and alleviate the saline injury by using natural products in garlic extract application on growth, metabolites, protein pattern and esterase enzyme of wheat plants. This study was conducted that wheat plant cv. Gimiza 11 response to osmotic stress effects and in general showed a variable response between different organs. The aerial parts of plants not only alleviated salinity injury but activated the fresh and dry matter productions. In root these parameters decreased as increasing salinity stress. Length of the shoots, roots and spikes run parallel with the previous results. Photosynthetic pigment enhanced markedly the increasing osmotic stress levels. The effect of garlic was reflected on the accumulation of soluble sugar and soluble protein in both roots and spikes, and a reduction of Na+ and an increase in K+ under garlic treatments were recorded. In the present study, staining intensity of protein bands of wheat plant was decreased as osmotic stress increased but the number of bands was increased up to -0.9 MPa, after that level a slight decrease was recorded (for control induction, 12 bands, -0.3 MPa, 16 bands, -0.6 MPa 14 bands, -0.9 14 bands, -1.2 MPa 11 bands and final 11 bands for -1.5 MPa). Induction protein bands for control plus garlic were 12 bands, for -0.3 MPa OSL plus garlic were 13 bands, for -0.6 MPa OSL plus garlic were 12 bands, for -0.9 MPa OSL plus garlic were 12 bands, for -1.2 MPa OSL were 8 bands and finally for -1.5 MPa OSL plus garlic were 9 bands. electrophoresis studies of esterase showed wide variations in their intensities and densities among all treatments. There were 6 isozymes forms of esterase under OSL and with garlic but intensity was different. It seems that garlic extract was able to enhance the tolerance of the wheat plant to osmotic stress.

关键词： Garlic Wheat SDS Page Esterase NaCl Level

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The Combined Action Strategy of Two Stresses, Salinity and Cu++ on Growth, Metabolites and Protein Pattern of Wheat Plant

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American Journal of Plant Sciences 2017年第3期8卷 625-643页

作者： Hamdia M. abd el-samad D. Mostafa Kholoud N. abd el-Hakeem Botany Department Faculty of Science Minia University El-Minia Egypt

The response of wheat plants to different osmotic stress levels varied among the different organs root, shoot and spike and the situation of these organs with application of two Cu++ levels 5 mM and 25 mM as CuSO4. The sensitivity of root organ was related with reduction in fresh, dry matter and length. This resulted from reduction of soluble sugar reflected a reduction in water uptake and K+ content of the cell sap. In the moderate organ spike, the reduction in fresh, dry matter and length were concomitant with the accumulation of soluble sugar and a huge accumulation of soluble protein. In the higher organ shoot, this related with more water uptake which in turn induced an accumulation of soluble protein and cofactor K+ content. It can be recorded that shoot was higher Na+ accumulation than root and spike. Data also showed further stimulatory effect on growth parameters by Cu++ applications with either concentration (7.5 mM and 25 mM). Irrigating the soil with either 7.5 or 25 mM CuSO4 induced a huge accumulation in soluble sugar, soluble protein and nitrate reductase. Cupper treatment with either concentration 7.5 mM or 25 mM induced a marked decrease in Na+ content at all OSL and has no significant change in the accumulation of K+ in both shoot and spike whereas induced a huge accumulation in root organ. The synthesis of protein bands with molecular weight 32.3 KDa at -1.5 MPa NaCl level treated with either 7.5 mM or 25 mM Cu++ concentrations was induced. Also the appearance of protein band with molecular weight 37KDa induced only at Cu++ treatments with 25 mM concentration in both control and under different osmotic levels (0.0, -0.3 MPa, -0.9 MPa, -1.5 MPa NaCl).

关键词： Osmotic Stress Levels Cupper Tolerance Wheat Protein Bands

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