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Numerical Simulatio. o. Saint-Venant Equatio.s with Thermal Energy dependency: Applicatio.s o. Glo.al Warming

o.en Jo.rnal o. Fluid Dynamics 2023年第4期13卷 191-205页

作者： raphael de o. garcia Graciele P. Silveira department o. Actuarial Science Federal University of S&atildeo Paulo Osasco Brazil department o. Physics Chemistry and Mathematics Federal University of S&atildeo Carlos Sorocaba Brazil

Since the Industrial Revo.utio., humanity has been intensifying the burning o. fo.sil fuels and as a co.sequence, the average temperature o. Earth has been increasing. The 20th century was the warmest and future pro.pects are no. favo.able, that is, even higher temperatures are expected. This demo.strates the impo.tance o. studies o. the subject, mainly to.predict po.sible enviro.mental, so.ial and eco.o.ic co.sequences. The o.jective o. this wo.k was to.identify the interference o. the increase in ambient temperature in the dynamics o. fluids, such as o.ean waves advancing o.er the co.tinent. Fo. this, thermal energy was co.sidered in the Saint-Venant equatio.s and co.putatio.al implementatio.s were perfo.med via Lax-Friedrichs and Adams-Mo.lto. metho.s. The results indicated that, in fact, depending o. the amo.nt o. thermal energy transferred to.the fluid, the advance o. water to.ards the co.tinent can o.cur, even in places where such a pheno.eno. has never been o.served.

关键词： Co.putatio.al Fluid Dynamics Saint-Venant Equatio.s Numerical Metho.s Glo.al Warming

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Numerical So.utio.s o. the Classical and Mo.ified Buckley-Leverett Equatio.s Applied to.Two.Phase Fluid Flo.

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o.en Jo.rnal o. Fluid Dynamics 2024年第3期14卷 184-204页

作者： raphael de o. garcia Graciele P. Silveira department o. Actuarial Science Federal University of So Paulo Osasco Brazil department o. Physics Chemistry and Mathematics Federal University of So Carlos Sorocaba Brazil

Climate change is a reality. The burning o. fo.sil fuels fro. o.l, natural gas and co.l is respo.sible fo. much o. the po.lutio. and the increase in the planet’s average temperature, which has raised discussio.s o. the subject, given the emergencies related to.climate. An energy transitio. to.clean and renewable so.rces is necessary and urgent, but it will no. be quick. In this sense, increasing the efficiency o. o.l extractio. fro. existing so.rces is crucial, to.avo.d waste and the drilling o. new wells. The purpo.e o. this wo.k was to.add diffusive and dispersive terms to.the Buckley-Leverett equatio. in o.der to.inco.po.ate extra pheno.ena in the tempo.al evo.utio. between the water-o.l and o.l-water transitio.s in the pipeline. Fo. this, the mo.ified Buckley-Leverett equatio. was discretized via essentially weighted no.-o.cillato.y schemes, co.pled with a three-stage Runge-Kutta and a fo.rth-o.der centered finite difference metho.s. Then, co.putatio.al simulatio.s were perfo.med and the results sho.ed that new features emerge in the transitio.s, when co.pared to.classical simulatio.s. Fo. instance, the dispersive term inhibits the diffusive term, adding o.cillatio.s, which indicates that the abso.ptio. o. the fluid by the po.o.s medium o.curs in a no.-ho.o.eneo.s manner. Therefo.e, based o. research such as this, decisio.s can be made regarding the replacement o. the po.o.s medium o. the insertio. o. new co.po.ents to.delay the replacement.

关键词： Co.putatio.al Fluid Dynamics Buckley-Leverett Equatio. Numerical Metho.s Two.phase Fluid Flo.

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Framewo.k to.Practical Intro.ucto.y Classes abo.t develo.ment o. Info.matio. System

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通讯和计算机（中英文版） 2016年第1期13卷 24-31页

作者： Ayrto. Saito.raphael garcia Mauricio.Araujo.Dias Miriam Maria Pedro.a Andreia Cristiane Silva Wiezzel UNESP-Univ. Estadual Paulista Roberto Simonsen 305 - Presidente Prudente Sao Paulo 19060 900 Brazil

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Plasmid instability when the hsp60 gene pro.o.er is used to.express the pro.ective no.-to.ic fragment B o. the diphtheria to.in in reco.binant BCG

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American Jo.rnal o. Mo.ecular Bio.o.y 2013年第2期3卷 81-86页

作者： Dilzamar V.Nascimento.o.ir A.dellago.tin raphael Hirata Jr Geraldo.M.B.Pereira Ana Luiza Matto.-Guaraldi Geraldo.R.garcia Armo. Faculdade de Ciencias Médicas Universidade do Estado do Rio de JaneiroRio de JaneiroBrazil Centro.de Bio.ecno.o.ia Universidade Federal de PelotasCampus UniversitárioPelotasBrazil Instituto.o.waldo.Cruz Fundacao Oswaldo CruzRio de JaneiroBrazil

The genetic mo.ificatio. o. the live attenuated Myco.acterium bo.is BCG to.deliver a pro.ective Co.ynebacterium diphtheriae antigen in vivo.co.ld be a safer and less co.tly alternative to.the new and mo.e expensive DTP vaccines available to.ay, in particular to.third wo.ld-co.ntries. The stability o. expressio. o. hetero.o.o.s antigens in BCG, ho.ever, is a majo. challenge to.the use o. live reco.binant bacteria in vaccine develo.ment and appears to.be dependent to.a certain extent, o. a genetic co.patibility between the expressio. cassette within the plasmid co.struct and the myco.acterium ho.t. In the quest fo. the best reco.binant BCG transfo.mant to.express the dtb gene o. C. diphtheriae we generated two.new rBCG strains by transfo.ming the Mo.eau substrain o. BCG with the myco.acterial expressio. vecto.s pUS973 and pUS977, each o.e carrying a different pro.o.er to.drive the expressio. o. the target antigen. After transfo.matio. reco.binant BCG clo.es were selected o. Middlebro.k 7H10 kanamycin Agar plates, expanded in Middlebro.k 7H9 kanamycin Bro.h and analyzed by agaro.e gel electro.ho.esis and immuno.lo.ting. rBCGs transfo.med with the co.struct carrying the weak PAN pro.o.er fro. M. paratuberculo.is stably expressed the dtb gene. Co.versely, rBCGs transfo.med with the co.struct carrying the stro.g myco.acterium hsp60 pro.o.er were unstable and co.sequently unfit fo. the expressio. o. the C. diphtheriae gene.

关键词： BCG Vaccine Reco.binant BCG Fragment B o. Diphtheria To.in Anti-Diphtheria Vaccine

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