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Myo-inositol supplement helps the performance of seawater-acclimated Nile tilapia,Oreochromis niloticus

Aquaculture and Fisheries 2024年第4期9卷 597-602页

作者： Behnam Foroutan Pattira Pongtippatee Chompoonut Kerdmusic Wanna Sirimanapong rapeepun vanichviriyakit Boonsirm Withyachumnarnkul Faculty of Innovative Agriculture and Fishery Establishment Project Prince of Songkla UniversitySurat Thani84100Thailand Veterinary Aquatic Animal Research Health Care Unit Department of Clinical Sciences and Public HealthFaculty of Veterinary ScienceMahidol UniversityNakhonpathom73170Thailand Department of Anatomy and Centex Shrimp Faculty of ScienceMahidol UniversityRama 6 Rd.Bangkok10400Thailand AquaAcademy Farm Tha ChanaSurat Thani84170Thailand Disciplines of Excellence in Sustainable Aquaculture Prince of Songkla UniversityHat YaiSongkhla90112Thailand

Seawater(SW)-acclimated Nile tilapia,Oreochromis niloticus,reared under a salinity 30 environment had lower growth and survival than the freshwater(FW)-acclimated *** was hypothesized that cells of the SW-acclimated fish had not been able to synthesize an adequate level of a compatible osmolyte,myo-inositol(MI),in adjusting to the salinity 30 *** this study,MI supplements,at 250,500,and 750 mg/kg pellets,were provided to the fish through *** the 30-day feeding trial,the following parameters were determined:final body weights;survival;biomass increase;feed conversion ratio(FCR);plasma osmolality and ions;and two transcripts in the gills mips250 and mipa1 encoding enzymes responsible for MI *** SW-acclimated *** receiving 500-mg MI supplement had significantly higher survival,biomass increase,and lower FCR than those of the SW-acclimated fish receiving no *** 500-mg MI supplemental level,the increasing values of plasma osmolality and Na+observed in SW-acclimated fish were significantly *** transcript mipa1,but not mips250,was markedly up-regulated in the SW-acclimated ***,compared with that of the FW-acclimated ***,MI at 500-mg supplement attenuated the up-regulation *** study suggests that MI supplement at the optimum level enhanced the performance of SW-acclimated ***,and through yet unknown mechanisms,attenuated some of their physiological responses to the osmotic stress.

关键词： Osmotic stress Seawater Nile tilapia Myo-inositol Mips Mipa

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移植前AS30D肝癌细胞状态决定其移植后在大白鼠体内的生长周期

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实验动物科学 2003年第Z1期23卷 34-页

作者：维帕威.维萨瓦托尔贾萨达.萨卡尔库卡莫尔波恩.朗格洛杰金达堪察那.肯库拉皮班.因班凯乌万塔尼.拉塔纳萨克达朗尼.布里帕克蒂.劳森国家实验动物中心研究和科学支持部国家实验动物中心质量监控部国家实验动物中心生产供应部泰国纳克思.帕特洪麦锡多大学 73170

Objective\ Pre transplant status of AS 30D hepatoma cell line was evaluated in Sprague Dawley rats. Cells from different sources, a) in vivo, from the other transplanted Sprague Dawley rats, b) in vitro, from cultured cell, and c) cryopreservation, from the liquid nitrogen storage tank, were transplanted into 5 week old female Sprague Dawley rats (respectively n=5, 3, and 5). Each animal received intraperitoneal injection of 0 5 ml cell suspension containing 1 5×10 6 cells. The results showed no significant difference among groups, in the quality parameters of cell transplantation, such as fluid volume, cell concentration, total cell, cell viability, and the daily consumption of food and water. However, the cell growth duration following the transplantation was lengthened at least one half fold or 5 days, when other sources of cells than the in vivo, were transplanted into rat abdominal cavity. Thus, the variety of pre transplant status of the AS 30D cells would enhance flexibility in cell preparations for the successful transplantation in different time frame.

关键词： AS-30D 肝癌细胞系移植 Sprague Dawley 大白鼠

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