检索结果-南通市图书馆

Constructing recombinant replication-defective adenoviral vectors that express glucose transporter-1 through in vitro ligation

引用

Neural Regeneration Research 2008年第4期3卷 456-460页

作者： Fangcheng Li Junliang Li ranyi liu Xinke Xu Kaichang Yuan Zhonghua Wu Department of Neurosurgery Second Hospital Affiliated to Sun Yat-sen University Guangzhou 510120 Guangdong Province China Central Laboratory for Prevention and Treatment of Tumor Sun Yat-sen University Guangzhou 510006 Guangdong Province China

BACKGROUND： We constructed a homologous recombination bacterial method based on the pAdEasy system, a widely used system, for generating recombinant adenoviral vectors that express glucose transporter- 1 （GLUT 1） in rats, OBJECTIVE： This study was designed to investigate the feasibility of generating recombinant replication-defective adenoviral vectors that express GLUT1 in rats by in vitro ligation based on the Adeno-X^TM system. DESIGN： An in vitro cell-based experiment. SETTING： This study was performed at the Linbaixin Medical Research Center of the Second Hospital Affiliated to Sun Yat-sen University and Central Laboratory for Prevention and Treatment of Tumor, Sun Yat-sen University between January and August 2004. MATERIALS： Male, adult, Sprague Dawley rats were used to extract total RNA from brain tissue. E. coli DH5 a and human embryonic kidney 293 cells （HEK293 cells） used in the present study were cryo-preserved by the Second Hospital Affiliated to Sun Yat-sen University. Rabbit anti-rat GLUT1 polyclonal antibody （Chemicon, U.S.A.） and primers （Shanghai Boya Bioengineering Co., Ltd） were also used. METHODS： E1/E3-deleted replication-defective adenoviral vectors were used. Using in vitro ligation, the target gene was first sub-cloned into a shuttle vector plasmid to obtain the fragment containing target gene expression cassettes by enzyme digestion. Subsequently, the fragment was co-transformed with linearized adenoviral backbone vector into the E. coli strain. The recombinant adenoviral plasmid was transfected into HEK293 cells to assembly recombinant adenoviral vectors with replication capabilities. The procedure was repeated several times for recombinant adenoviral vectors amplification. MAIN OUTCOME MEASURES： Efficiency of recombinant adenoviral vectors to express the target gene was measured by gene and protein expression through polymerase chain reaction and Western Blot assays, respectively. RESULTS： Results demonstrated that recombinant adenoviral vectors successfully expressed GLUT1 protein, wi

关键词： glucose transporter-1 cloning recombinant adenoviral vector

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Translational oncology toward benefiting cancer patients:the Sun Yat-sen University Cancer Center experience

引用

Science China(Life Sciences) 2016年第10期59卷 1057-1062页

作者： Mathilde Guerin Chaonan Qian Qian Zhong Qian Cui Yunmiao Guo Jinxin Bei Jianyong Shao Xiaofeng Zhu Wenlin Huang Jiangxue Wu ranyi liu Qiang liu Jing Wang Weihua Jia Xiaohui Zheng Yixin Zeng Sun Yat-sen University Cancer Center Guangzhou 510060China

Cancer is a leading cause of death in China with an estimation of nearly 2 million deaths every year(Chen and Fu,2011b).Matter of a public health importance in China and worldwide,the scientific community is still facing

关键词：癌症患者中山大学肿瘤转化器官特异性多因子疾病公共卫生抗癌药物

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Synthetic lethal short hairpin RNA screening reveals that ring finger protein 183 confers resistance to trametinib in colorectal cancer cells

引用

Chinese Journal of Cancer 2017年第12期36卷 726-736页

作者： Rong Geng Xin Tan Zhixiang Zuo Jiangxue Wu Zhizhong Pan Wei Shi ranyi liu Chen Yao Gaoyuan Wang Jiaxin Lin Lin Qiu Wenlin Huang Shuai Chen State Key Laboratory of Oncology in South China Collaborative Innovation Center of Cancer MedicineSun Yat-sen University Cancer Center651 Dongfeng Road EastGuangzhou 510507GuangdongP.R.China Department of Experimental Research Sun Yat-sen University Cancer CenterGuangzhou 510060GuangdongP.R.China Department of Colorectal Surgery Sun Yat-sen University Cancer CenterGuangzhou 510060GuangdongP.R.China Guangdong Esophageal Cancer Institute Sun Yat-sen University Cancer CenterGuangzhou 510060GuangdongP.R.China Guangdong Provincial Key Laboratory of Tumor.Targeted Drugs and Guangzhou Enterprise Key Laboratory of Gene Medicine Guangzhou Doublle Bioproducts Co.Ltd.Guangzhou 510507GuangdongP.R.China

Background: The mitogen-activated extracellular signal-regulated kinase 1/2(MEK1/2) inhibitor trametinib has shown promising therapeutic effects on melanoma, but its efficacy on colorectal cancer(CRC) is limited. Synthetic lethality arises with a combination of two or more separate gene mutations that causes cell death, whereas individual mutations keep cells alive. This study aimed to identify the genes responsible for resistance to trametinib in CRC cells,using a synthetic lethal short hairpin RNA(shRNA) screening approach.Methods: We infected HT29 cells with a pooled lentiviral shRNA library and applied next-generation sequencing to identify shRNAs with reduced abundance after 8-day treatment of 20 nmol/L trametinib. HCT116 and HT29 cells were used in validation studies. Stable ring finger protein 183(RNF183)-overexpressing cell lines were generated by pcDNA4-myc/his-RNF183 transfection. Stable RNF 183-knockdown cell lines were generated by infection of lentiviruses that express RNF183 shRNA, and small interference RNA(siRNA) was used to knock down RNF183 transiently.Quantitative real-time PCR was used to determine the mRNA expression. Western blotting, immunohistochemical analysis, and enzyme-linked immunosorbent assay(ELISA) were used to evaluate the protein abundance. MTT assay,colony formation assay, and subcutaneous xenograft tumor growth model were used to evaluate cell proliferation.Results: In the primary screening, we found that the abundance of RNF183 shRNA was markedly reduced after treatment with trametinib. Trametinib induced the expression of RNF183, which conferred resistance to drug-induced cell growth repression and apoptotic and non-apoptotic cell deaths. Moreover, interleukin-8(IL-8) was a downstream gene of RNF183 and was required for the function of RNF183 in facilitating cell growth. Additionally, elevated RNF183 expression partly reduced the inhibitory effect of trametinib on IL-8 expression. Finally, xenograft tumor model showed the synergism of RNF183 knockdown and trametinib in repres

关键词： Synthetic lethal Colorectal cancer Ring finger protein 183 Mitogen-activated extracellular signalregulated kinase 1/2 Trametinib

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

介电损耗在TFT性能提升中的应用

引用

创新创业理论研究与实践 2023年第20期6卷 1-6页

作者：李砚秋林鸿涛颜京龙崔晓鹏陈维涛周冉一刘丹方亮北京京东方显示技术有限公司北京100176 重庆京东方光电科技有限公司重庆400700 重庆大学重庆400044

该文分析表征了TFT器件栅极绝缘层的介电性能,建立介质层介电损耗与TFT耐压强度、电学性能的关系。首先制备栅极、栅极绝缘层、Si构成的MIS电容,优选出最佳测试参数组合;然后测试电容介电损耗和击穿电压,分析介电损耗和耐压强度的关系;... 详细信息

该文分析表征了TFT器件栅极绝缘层的介电性能,建立介质层介电损耗与TFT耐压强度、电学性能的关系。首先制备栅极、栅极绝缘层、Si构成的MIS电容,优选出最佳测试参数组合;然后测试电容介电损耗和击穿电压,分析介电损耗和耐压强度的关系;再以MIS电容制备工艺为基础,制备TFT器件并测试后TFT转移曲线,分析介电损耗和TFT开态电流Ion的关系。实验结果表明:介电损耗和耐压强度及TFT特性呈负相关关系,介质损耗增加(0.03%→0.40%),击穿电压降低(334V→300V),Ion下降(3.99uA→3.48uA)。温度、频率、湿度均会影响介电损耗测试。该文明确了最佳测量参数组合,开发出一套可以表征TFT栅极绝缘层介电损耗的方法。栅极绝缘层的介电损耗和TFT耐压强度、电学性能相关,可以通过该介质层的介电损耗多维度表征TFT性能,为TFT性能分析表征、优化提供参考。

关键词：介电损耗击穿电压 TFT特性栅极绝缘层 TFT耐压强度 MIM器件

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

E1B缺陷溶瘤腺病毒dl1520体外对鼻咽癌细胞的杀伤作用

引用

华中医学杂志 2005年第3期29卷 183-184页

作者：彭吉林罗慧玲刘然义郧阳医学院免疫教研室中山大学肿瘤防治中心肿瘤研究所

目的探讨E1B55KDa缺陷腺病毒dl1520体外对鼻咽癌细胞的杀伤作用。方法dl1520感染不同鼻咽癌株后测定病毒增殖;观察细胞病变效应;MTT法测定细胞生长抑制作用。结果dl1520在鼻咽癌细胞中可复制增殖并有致细胞病变效应,MTT法测定感染复数为... 详细信息

目的探讨E1B55KDa缺陷腺病毒dl1520体外对鼻咽癌细胞的杀伤作用。方法dl1520感染不同鼻咽癌株后测定病毒增殖;观察细胞病变效应;MTT法测定细胞生长抑制作用。结果dl1520在鼻咽癌细胞中可复制增殖并有致细胞病变效应,MTT法测定感染复数为0.032、0.16、0.8、4、20、100时CNE2细胞生长抑制率分别为2.78%、7.41%、10.19%、24.07%、45.37%、67.59%。结论dl1520体外可在鼻咽癌细胞中复制并裂解杀伤肿瘤细胞。

关键词： dl1520 鼻咽癌细胞杀伤作用溶瘤腺病毒体外缺陷致细胞病变效应细胞生长抑制率生长抑制作用杀伤肿瘤细胞 MTT法病毒增殖感染复数 CNE2 测定可复制

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：