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Comparative Genome Analysis of Scutellaria baicalensis and Scutellaria barbata Reveals the Evolution of Active Flavonoid Biosynthesis

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Genomics, Proteomics & Bioinformatics 2020年第3期18卷 230-240页

作者： Zhichao Xu ranran gao Xiangdong Pu Rong Xu Jiyong Wang Sihao Zheng Yan Zeng Jun Chen Chunnian He Jingyuan Song Key Lab of Chinese Medicine Resources Conservation State Administration of Traditional Chinese Medicine of ChinaInstitute of Medicinal Plant DevelopmentChinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing 100193China Engineering Research Center of Chinese Medicine Resource Ministry of EducationBeijing 100193China China National Traditional Chinese Medicine Co. LtdBeijing 102600China

Scutellaria baicalensis(***)and Scutellaria barbata(***)are common medicinal plants of the Lamiaceae *** produce specific flavonoid compounds,including baicalein,scutellarein,norwogonin,and wogonin,as well as their glycosides,which exhibit antioxidant and antitumor ***,we report chromosome-level genome assemblies of *** and *** with quantitative chromosomal variation(2 n=18 and 2 n=26,respectively).The divergence of *** and *** occurred far earlier than previously reported,and a whole-genome duplication(WGD)event was *** insertion of long terminal repeat elements after speciation might be responsible for the observed chromosomal expansion and *** genome analysis of the congeneric species revealed the species-specific evolution of chrysin and apigenin biosynthetic genes,such as the ***-specific tandem duplication of genes encoding phenylalanine ammonia lyase and chalcone synthase,and the ***-specific duplication of genes encoding 4-Co A *** addition,the paralogous duplication,colinearity,and expression diversity of CYP82 D subfamily members revealed the functional divergence of genes encoding flavone hydroxylase between *** and *** these Scutellaria genomes reveals the common and species-specific evolution of flavone biosynthetic ***,these findings would facilitate the development of molecular breeding and studies of biosynthesis and regulation of bioactive compounds.

关键词： Scutellaria Whole-genome duplication Flavonoid biosynthesis Tandem duplication Species-specific evolution

Promoter variations in DBR2-like affect artemisinin production in different chemotypes of Artemisia annua

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Horticulture Research 2023年第9期10卷 220-232页

作者： Xingwen Wang Lan Wu Li Xiang ranran gao Qinggang Yin Mengyue Wang Zhaoyu Liu Liang Leng Yanyan Su Huihua Wan Tingyu Ma Shilin Chen Yuhua Shi Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine Artemisinin Research CenterInstitute of Chinese Materia MedicaChina Academy of Chinese Medical SciencesBeijing 100700China Institute of Herbgenomics Chengdu University of Traditional Chinese MedicineChengdu 611137China Amway(China)Botanical R&D Center Wuxi 214115China Key Lab of Chinese Medicine Resources Conservation State Administration of Traditional Chinese Medicine of the People’s Republic of ChinaInstitute of Medicinal Plant DevelopmentChinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing 100193China

Artemisia annua is the only known plant source of the potent antimalarial artemisinin,which occurs as the low-and high-artemisinin producing(LAP and HAP)***,the different mechanisms of artemisinin producing between these two chemotypes were still not fully ***,we performed a comprehensive analysis of genome resequencing,metabolome,and transcriptome data to systematically compare the difference in the LAP chemotype JL and HAP chemotype *** analysis revealed that 72.18%of sesquiterpenes was highly accumulated in HAN compared to *** omics analysis found a DBR2-Like(DBR2L)gene may be involved in artemisinin ***2L was highly homologous with DBR2,belonged to ORR3 family,and had the DBR2 activity of catalyzing artemisinic aldehyde to dihydroartemisinic *** resequencing and promoter cloning revealed that complicated variations existed in DBR2L promoters among different varieties of *** and were clustered into three variation *** promoter activity of diverse variant types showed obvious ***,the core region(-625 to 0)of the DBR2L promoter was identified and candidate transcription factors involved in DBR2L regulation were ***,the result indicates that DBR2L is another key enzyme involved in artemisinin *** promoter variation in DBR2L affects its expression level,and thereby may result in the different yield of artemisinin in varieties of *** provides a novel insight into the mechanism of artemisinin-producing difference in LAP and HAP chemotypes of ***,and will assist in a high yield of artemisinin in ***.

关键词： thereby insight homologous

Web-Based Data Management and Sharing System for Electron Proke Micro-analysis

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Journal of Donghua University(English Edition) 2018年第4期35卷 315-320页

作者： HE Yutong TIAN Di gao ranran FAN Runlong YAO Li CHEN Pengfei College of Instrumentation & Electrical Engineering Jilin University Jilin 130021 China Institute of Geology Chinese Academy of Geological Sciences Beijing 100037 China College of Earth Sciences Jilin University Jilin 130061 China The 41st Research Institute of CETC Qingdao 266555 China

This paper proposes a useful web-based system for the management and sharing of electron probe micro-analysis( EPMA)data in geology. A new web-based architecture that integrates the management and sharing functions is developed and *** scientists can utilize this system to not only manage their data,but also easily communicate and share it with other *** query methods provide the core functionality of the proposed management and sharing modules. The modules in this system have been developed using cloud GIS technologies,which help achieve real-time spatial area retrieval on a map. The system has been tested by approximately 263 users at Jilin University and Beijing SHRIMP Center. A survey was conducted among these users to estimate the usability of the primary functions of the system,and the assessment result is summarized and presented.

关键词： electron probe micro-analysis ( EPMA ) data management data sharing web-based architecture data query GIS

Crocus genome reveals the evolutionary origin of crocin biosynthesis

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Acta Pharmaceutica Sinica B 2024年第4期14卷 1878-1891页

作者： Zhichao Xu Shanshan Chen Yalin Wang Ya Tian Xiaotong Wang Tianyi Xin Zishan Li Xin Hua Shengnan Tan Wei Sun Xiangdong Pu Hui Yao ranran gao Jingyuan Song Key Lab of Chinese Medicine Resources Conservation State Administration of Traditional Chinese Medicine of the People's Republic of ChinaInstitute of Medicinal Plant DevelopmentChinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing 100193China State Key Laboratory of Basis and New Drug Development of Natural and Nuclear Drugs Engineering Research Center of Chinese Medicine ResourceMinistry of EducationBeijing 100193China College of Life Science Northeast Forestry UniversityHarbin 150040China Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine China Academy of Chinese Medical SciencesInstitute of Chinese Materia MedicaBeijing 100700China Analysis and Testing Center of Northeast Forestry University Harbin 150040China School of Pharmacy Anhui Medical UniversityInflammation and Immune Mediated Diseases Laboratory of Anhui ProvinceHefei 230032China

Crocus sativus (saffron) is a globally autumn-flowering plant, and its stigmas are the most expensive spice and valuable herb medicine. Crocus specialized metabolites, crocins, are biosynthesized in distant species, Gardenia (eudicot) and Crocus (monocot), and the evolution of crocin biosynthesis remains poorly understood. With the chromosome-level Crocus genome assembly, we revealed that two rounds of lineage-specific whole genome triplication occurred, contributing important roles in the production of carotenoids and apocarotenoids. According to the kingdom-wide identification, phylogenetic analysis, and functional assays of carotenoid cleavage dioxygenases (CCDs), we deduced that the duplication, site positive selection, and neofunctionalization of Crocus-specific CCD2 from CCD1 members are responsible for the crocin biosynthesis. In addition, site mutation of CsCCD2 revealed the key amino acids, including I143, L146, R161, E181, T259, and S292 related to the catalytic activity of zeaxanthin cleavage. Our study provides important insights into the origin and evolution of plant specialized metabolites, which are derived by duplication events of biosynthetic genes.

关键词： Crocus sativus WGT Crocin biosynthesis Carotenoids Apocarotenoids CCDs

西红花苷生源途径解析及其合成生物学研究进展

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科学通报 2021年第2期66卷 219-232页

作者：浦香东徐志超高冉冉宋经元中国医学科学院北京协和医学院药用植物研究所国家中医药管理局中药资源保护重点研究室北京100193 中药资源教育部工程研究中心北京100193 中国医学科学院北京协和医学院药用植物研究所云南分所景洪666100

天然产物是新药研发的重要资源,具有显著的抗疟疾、抗肿瘤、抗病毒感染等作用.珍稀名贵中药西红花的活性成分为西红花苷,属于脱辅基类胡萝卜素类化合物,具有保护中枢神经系统及预防心脑血管疾病的药理活性.西红花苷资源匮乏、价格高昂,... 详细信息

天然产物是新药研发的重要资源,具有显著的抗疟疾、抗肿瘤、抗病毒感染等作用.珍稀名贵中药西红花的活性成分为西红花苷,属于脱辅基类胡萝卜素类化合物,具有保护中枢神经系统及预防心脑血管疾病的药理活性.西红花苷资源匮乏、价格高昂,极大限制其药物开发及临床应用.本文从西红花苷的化学表征、生物合成途径解析及合成生物学研究进行综述,展示了西红花苷的光谱学和质谱学特征,阐述其完整生物合成途径的解析,介绍了利用微生物细胞工厂生产5种西红花苷的最新突破.据此,进一步提出开展不同物种西红花苷生物合成途径关键酶的进化机制、合成生物学关键技术探索和条件优化等研究,以推动西红花苷工业化生产及新药研发,为西红花苷来源的中药资源可持续发展提供理论基础和方法指导.

关键词：西红花苷化学表征生物合成途径合成生物学西红花栀子

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C/C复合材料的抗氧化研究

材料导报 2013年第S1期27卷 378-381页

作者：高冉冉王成国陈旸山东大学材料科学与工程学院碳纤维工程技术研究中心

C/C复合材料有多种优异性能,但是在高温含氧条件下易于氧化。为了能充分发挥其优良作用,必须进行抗氧化处理。目前国内外主要的抗氧化方法中,涂层法与纤维改性法和基体改性法相比,是目前最有效的抗氧化方法。分析比较了玻璃涂层、陶瓷... 详细信息

C/C复合材料有多种优异性能,但是在高温含氧条件下易于氧化。为了能充分发挥其优良作用,必须进行抗氧化处理。目前国内外主要的抗氧化方法中,涂层法与纤维改性法和基体改性法相比,是目前最有效的抗氧化方法。分析比较了玻璃涂层、陶瓷涂层、金属涂层以及复合涂层4种抗氧化体系的研究现状,展望了发展抗氧化涂层的意义及前景。

关键词： C/C复合材料抗氧化改性涂层

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二维光子晶体传输特性的时域精细积分法

动力学与控制学报 2019年第4期17卷 299-305页

作者：杨红卫彭硕高冉冉北京工业大学应用数理学院

本文研究了二维光子晶体的时域精细积分法,并对其精度、效率、以及稳定性进行分析.从麦克斯韦方程的微分形式出发,利用Yee元胞将空间微分算子近似为差分算子,结合边界条件及激励源的表达式得到一组关于时间的常微分方程.对时间进行精细... 详细信息

本文研究了二维光子晶体的时域精细积分法,并对其精度、效率、以及稳定性进行分析.从麦克斯韦方程的微分形式出发,利用Yee元胞将空间微分算子近似为差分算子,结合边界条件及激励源的表达式得到一组关于时间的常微分方程.对时间进行精细划分,使用精细积分算法求解常微分方程组.结合通解与激励源的特解得到光子晶体两端的反射场及透射场分布,进而通过傅里叶变换求得二维光子晶体的传输特性.数值算例表明,本文方法具有准确、稳定、高效的特点.

关键词：二维光子晶体光子带隙时域精细积分法时域有限差分法传输特性

持续或间断应力载荷下正畸微种植体稳定性的组织形态学和生物力学评价

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中国组织工程研究 2019年第22期23卷 3488-3494页

作者：吴也可郜然然赵立星成都中医药大学附属医院四川省成都市610072 四川大学华西口腔医学院四川省成都市610041

背景:在正畸治疗过程中,很多学者认为间断力相比持续力能获得同等甚至更大的牙移动疗效,并可减少牙根吸收等不良反应的发生,然而上述结论多来自体外细胞实验或临床个案观察,尚缺少设计科学合理、规模较大的动物实验。目的:分析持续应力... 详细信息

背景:在正畸治疗过程中,很多学者认为间断力相比持续力能获得同等甚至更大的牙移动疗效,并可减少牙根吸收等不良反应的发生,然而上述结论多来自体外细胞实验或临床个案观察,尚缺少设计科学合理、规模较大的动物实验。目的:分析持续应力或两种模式间断应力载荷对正畸微种植体骨整合及生物稳定性的影响。方法:取48只Beagle犬(四川大学实验动物中心提供),在下颌骨两侧第一磨牙和第二前磨牙的根间区域各植入1枚微种植体(每只犬共植入4枚微种植体),随机分4组:持续组每个加载周期连续加载应力,间断A组在每个加载周期的最后3d中断应力,间断B组在每个加载周期的最后7d中断应力,以未加力组为对照。应力加载方法与周期:通过结扎丝在同侧2个微种植体安装镍钛螺旋拉簧,实施加载力;设置1个加载周期为2周,共加载4个周期。加载2,4,6,8周后,获取各组下颌骨组织,进行显微CT、组织学观察和力学拉拔测试。实验方案经过四川大学实验动物伦理委员会批准。结果与结论:①未加力组加载不同时间点的峰值拉拔力均高于持续组(P<0.05),间断B组加载2,4,6周后的峰值拉拔力均高于持续组(P<0.05),间断A组加载2周后的峰值拉拔力均高于持续组(P<0.05);②显微CT显示相同加载时间点下,未加力组骨整合、骨体积分数、相交表面均高于持续组(P<0.05);间断B组加载2,4,6,8周后的骨整合、骨体积分数高于持续组(P<0.05),加载2,4,6周后的相交表面高于持续组(P<0.05);间断A组加载2,4周后的骨整合高于持续组(P<0.05),加载2,4,6周后的骨体积分数高于持续组(P<0.05),加载2周的相交表面高于持续组(P<0.05);③加载8周后,各组均可见骨重塑现象,骨重塑由好到差的排序依次为:未加力组、间断B组、间断A组、持续组;④结果表明,间断应力相比持续应力加载更有利于微种植体获得良好的稳定性,且7d/7d间断加载周期较11d/3d方案更能促进骨-微种植体结合。

关键词：微种植体持续应力间断应力骨整合稳定性峰值拉拔力动物实验显微CT

自噬与中医药延缓卵巢衰老的相关性研究进展与评述

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中华中医药学刊 2017年第12期35卷 3117-3120页

作者：白俊郜然然吴克明成都中医药大学四川成都610075

随着现代社会越来越多的女性推迟生育年龄和二胎政策全面放开,高龄生育问题给生殖领域和妇产科带来了前所未有的挑战。尤其是受年龄影响,因卵巢逐渐衰老导致女性生育力下降的问题倍受关注。近年来,自噬作为衰老过程中一种重要的调节机... 详细信息

随着现代社会越来越多的女性推迟生育年龄和二胎政策全面放开,高龄生育问题给生殖领域和妇产科带来了前所未有的挑战。尤其是受年龄影响,因卵巢逐渐衰老导致女性生育力下降的问题倍受关注。近年来,自噬作为衰老过程中一种重要的调节机制成为了新的研究热点,多项研究中已证明了增强自噬可以延缓衰老,同时也有研究发现在卵泡的形成,发育及闭锁的环节中都有细胞自噬参与的现象,颗粒细胞中自噬过程也是相对活跃的。因此,细胞自噬可能对女性生殖功能有着重要影响。通对细胞自噬与卵巢衰老之间的微观联系研究,为补肾中药改善卵巢功能和延缓卵巢衰老提供新的理论依据。

关键词：自噬中医药延缓卵巢衰老