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In vitro gene silencing of independent phosphoglycerate mutase (iPGM) in the filarial parasite Brugia malayi

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Infectious Diseases of Poverty 2013年第1期2卷 27-38页

作者： Prashant Kumar Singh Susheela Kushwaha Shahab Mohd Manisha Pathak Shailja misra-Bhattacharya Division of Parasitology CSIR-Central Drug Research InstituteB.S.10/1Sector 10Jankipuram ExtensionSitapur RoadLucknow 226021UPIndia

Background:The phosphoglycerate mutase(PGM)enzyme catalyzes the interconversion of 2-and 3-phosphoglycerate in the glycolytic/gluconeogenic pathways that are present in the majority of cellular *** can be classified as cofactor-dependent PGM(dPGM)or cofactor-independent PGM(iPGM).Vertebrates,yeasts,and many bacteria have only dPGM,while higher plants,nematodes,archaea,and many other bacteria have only iPGM.A small number of bacteria,including Escherichia coli and certain archaea and protozoa,contain both *** silencing of ipgm in Caenorhabditis elegans(***)has demonstrated the importance of this enzyme in parasite viability and,therefore,its potential as an anthelmintic drug *** this study,the role of the Brugia malayi(***)ipgm in parasite viability,microfilaria release,embryogenesis,and in vivo development of infective larvae post-gene silencing was explored by applying ribonucleic acid(RNA)interference ***:The in vitro ipgm gene silencing by small interfering RNA(siRNA)leads to severe phenotypic deformities in the intrauterine developmental stages of female worms with a drastic reduction(~90%)in the motility of adult parasites and a significantly reduced(80%)release of microfilariae(mf)by female worms in *** half of the in vitro-treated infective L3 displayed sluggish *** in vivo survival and development of siRNA-treated infective larvae(L3)was investigated in the peritoneal cavity of jirds where a~45%reduction in adult worm establishment was ***:The findings clearly suggest that iPGM is essential for both larval and adult stages of *** parasite and that it plays a pivotal role in female worm *** results thus validate the Bm-iPGM as a putative anti-filarial drug target.

关键词： siRNA Lymphatic filariasis Drug target RNAi Embryogenesis

Downregulation of miRNA-21 and cancer stem cells after chemotherapy results in better outcome in breast cancer patients

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World Journal of Stem Cells 2022年第4期14卷 310-313页

作者： Shailendra Dwivedi Puneet Pareek Jeewan Ram Vishnoi Praveen Sharma Sanjeev misra Biochemistry All India Institute of Medical Sciences Gorakhpur IndiaGorakhpur 273008Uttar PradeshIndia Department of Radiation Oncology All India Institute of Medical SciencesJodhpur 342005RajasthanIndia Surgical Oncology All India Institute of Medical SciencesJodhpur 342005RajasthanIndia Department of Biochemistry All India Institute of Medical SciencesJodhpur 342005RajasthanIndia Department of Surgical Oncology All India Institute of Medical SciencesJodhpur 342005RajasthanIndia King George’s Medical University Lucknow 226003Uttar PradeshIndia

Epigenetic modifications have been observed as a decline in miRNA-21 expression and breast cancer stem cell(CSC)population after 3 cycles of standard *** epigenetic response(miRNAs expression)and CSCs are also correlated in patients with Breast *** patients who tolerated chemotherapy well,miRNA-21(non-coding RNA)expression decreased significantly after three cycles of *** miRNA-21 expression in breast cancer tissue was quantified by quantitative PCR(real-time PCR)using the standard *** addition,breast CSCs(CD44+/CD24-)were also decreased in these *** miRNA-21 regulates cell division,proliferation,and autophagy of cancerous cells(as it targets phosphatase and tensin homolog/AKT/transcription factor EB/programmed cell death 4/autophagy-related protein 5 and chemotherapy also produces similar effects),thereby contributing to these ***,when all of the targets on genes have been explored by mimic miRNA,chemotherapy combined with anti-miRNA21 therapy may prove useful in the care of cancer patients.

关键词： Epigenetic modification miRNA-21 Breast carcinoma Autophagy Chemotherapy Breast cancer stem cells

Correlation between microstructure and impact toughness of weld heat-affected zone in 5 wt.% manganese steels

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Journal of Iron and Steel Research International 2019年第7期26卷 761-770页

作者： Jun-hui Li Hong-hong Wang Qiang Luo Li Li Chao Sun R.D.K. misra The State Key Laboratory of Refractories and Metallurgy Wuhan University of Science and Technology Wuhan 430081 Hubei China Research Institute of Nanjing Iron and Steel Co. Ltd. Nanjing 210035 Jiangsu China Laboratory for Excellence in Advanced Steel Research Department of Metallurgical Materials and Biomedical Engineering University of Texas at El Paso El Paso TX 7996& USA

The microstructure in welding heat-affected zones of 5 wt.% manganese steels was studied, and its effect on impact toughness was analyzed. The simulated coarse-grained heat-affected zone (CGHAZ) had the lowest impact toughness of ~39 J at — 40℃ because of coarse-grained structure and least volume fraction of retained austenite (RA) of 1.2 vol.%. The impact toughness of simulated intercritical heat-affected zone (ICHAZ) and fine-grained heat-affected zone (FGHAZ) were ~165 and ~45 J, respectively, at — 40℃. The effective grain size of simulated FGHAZ was smaller than that of the simulated ICHAZ. Furthermore, microstructural investigation revealed that the simulated FGHAZ and ICHAZ had similarity in volume fraction and stability of RA. However, tempered martensite was present in ICHAZ and absent in FGHAZ. It is proposed that the presence of tempered martensite contributed to good impact toughness in simulated ICHAZ.

关键词： 5 wt.% manganese steel Welding heat-affected zone Retained austenite Tempered martensite Impact toughness

Enhanced thermoelectric performance of Bi_(0.5)Sb_(1.5)Te_(3) via Ni-doping:A Shift of peak ZT at elevated temperature via suppressing intrinsic excitation

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Journal of Materiomics 2021年第6期7卷 1264-1274页

作者： Sahiba Bano D.K.misra J.S.Tawale Sushil Auluck CSIR-National Physical Laboratory Dr.K.S.Krishnan MargNew Delhi110012India Academy of Scientific&Innovative Research(AcSIR) CSIR-NPL CampusNew Delhi110012India

Bi_(2)Te_(3)-based thermoelectric(TE)materials have been demonstrated to be a potential candidate for mainly thermoelectric cooling/refrigeration ***,minority charge carriers excitation at high temperature reduces thermopower which restricts these materials for the use in power *** present work,substitution of Ni on Sb site in Bi_(0.5)Sb_(1.5-x)NixTe_(3)(x=0,0.01,0.04 and 0.08)actuates the system to supress the intrinsic excitation leading to shift in highest ZT to higher temperature *** Density functional theory(DFT)calculations and experimental results reveal that Ni in Bi_(0.5)Sb_(1.5)Te3 provides the extra holes and slightly reduces the band gap Eg which enhances the s of Ni-doped Bi_(0.5)Sb_(1.5-x)NixTe_(3) samples and a at elevated ***,Ni-doping in Bi_(0.5)Sb_(1.5)Te_(3) also reduces kL which is attributed to the phonon scattering due to mass fluctuations and microstructural features such as grain boundary and strain field domain observed from HRTEM *** favourable condition leads to maximum ZT~1.38 at 433K for Bi_(0.5)Sb_(1.46)Ni_(0.04)Te_(3) and ZT_(avg)~1.1 between 300K and *** the calculated theoretical TE conversion device efficiency h of Bi_(0.5)Sb_(1.46)Ni_(0.04)Te3(η~5.5%)was achieved to be nearly twice than the efficiency of matrix Bi_(0.5)Sb_(1.5)Te3(h~3%).Experimental electronic transport is well corroborated with theoretically estimated DFT results.

关键词： Density functional theory Electronic transport Electron microscopy Bipolar thermal conductivity Thermoelectric performance and quality factor

Laser optogalvanic investigations of the Neon 6302 A transition

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原子与分子物理学报 2007年第B8期24卷 102-104页

作者： HAN Xianming L POZDIN V HARIDASS C misra P Department of Physics ＆ Astronomy Butler University Indianapolis IN 46208 CLOE Comell University Ithaca NY 14850 Department of Physical Sciences Belfry School Belfry KY 41514 Department of Physics ＆ Astronomy Howard University Washington D. C20059

The optogalvanic effect of neon, in the presence of CO gas, has been specifically investigated for the 630.2 nm transition. Our theoretical model, based on rate equations, predicts well the observed signals as a sum o... 详细信息

关键词：光电效应信号波形蒙特卡罗法激光

Effects of Nb and V on Microstructural Evolution,Precipitation Behavior and Tensile Properties in Hot-rolled Mo-bearing Steel

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Journal of Iron and Steel Research International 2016年第6期23卷 559-565页

作者： Fu-ming LIU Jian-jun WANG Yu-jie LIU R.D.K.misra Chun-ming LIU School of Materials Science and Engineering Northeastern University Key Laboratory for Anisotropy and Texture of Materials of Ministry of Education Northeastern University Laboratory for Excellence in Advanced Steel Research Department of Metallurgical and Materials EngineeringUniversity of Texas at El Paso

Microstructural evolution,precipitation behavior,and tensile properties of four experimental Mo-bearing steels were studied to elucidate the effects of Nb and V on microstructural *** results indicated that the microstructure of hot-rolled steels consisted of polygonal ferrite and degenerate pearlite,and the morphology remained same after holding at 600 ℃for *** smallest grain size was obtained in Nb-V-Mo containing steel,followed by Nb-Mo and V-Mo *** was less in the hot-rolled Mo-bearing *** maximum volume fraction of precipitates was obtained in Nb-V-Mo bearing steel,and the Nb-V-Mo complex carbonitrides were highly stable at 600℃.In the Mo-bearing steel,the yield ratio was lowest at room temperature but highest at 600℃,which was attributed to the precipitation of Mo carbides and drag effect of Mo solute on dislocation movement.

关键词： rolled steels Precipitation tensile solute attributed polygonal holding ferrite dislocation

Development of a field enhanced photocatalytic device for biocide of coliform bacteria

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Journal of Environmental Sciences 2016年第6期28卷 38-44页

作者： Jeff M.Huber Krista L.Carlson Otakuye Conroy-Ben Mano misra Swomitra K.Mohanty Departments of Civil Engineering University of Utah Departments of Metallurgical Engineering University of Utah Departments of Chemical Engineering University of Utah

A field enhanced flow reactor using bias assisted photocatalysis was developed for bacterial disinfection in lab-synthesized and natural waters. The reactor provided complete inactivation of contaminated waters with flow rates of 50 mL/min. The device consisted of titanium dioxide nanotube arrays, with an externally applied bias of up to 6 V. Light intensity, applied voltage, background electrolytes and bacteria concentration were all found to impact the device performance. Complete inactivation of Escherichia coli W3110（- 8 × 10^3CFU/mL） occurred in 15 sec in the reactor irradiated at 25 mW/cm^2 with an applied voltage of 4 V in a 100 ppm NaCl solution. Real world testing was conducted using source water from Emigration Creek in Salt Lake City, Utah. Disinfection of natural creek water proved more challenging, providing complete bacterial inactivation after 25 sec at 6 V. A reduction in bactericidal efficacy was attributed to the presence of inorganic and organic species, as well as the increase in robustness of natural bacteria.

关键词： Titanium dioxide Nanotubes Immobilized Photocatalysis Escherichia coli Inactivation

FOXO3a as a sensor of unilateral nerve injury in sensory neurons ipsilateral, contralateral and remote to injury

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Neural Regeneration Research 2020年第12期15卷 2353-2361页

作者： Jovan CDHasmatali Jolly De Guzman Jayne MJohnston Hossein Noyan Bernhard HJuurlink Vikram misra Valerie MKVerge Department of Anatomy Physiologyand PharmacologyUniversity of SaskatchewanSaskatoonSKCanada Cameco MS Neuroscience Research Center University of SaskatchewanSaskatoonSKCanada Department of Veterinary Microbiology University of SaskatchewanSaskatoonSKCanada

Emerging evidence supports that the stress response to peripheral nerve injury extends beyond the injured neuron,with alterations in associated transcription factors detected both locally and remote to the ***-induced nuclear translocation of the transcription factor forkhead class box O3a(FOXO3a)was initially linked to activation of apoptotic genes in many neuronal ***,a more complex role of FOXO3a has been suggested in the injury response of sensory neurons,with the injured neuron expressing less *** elucidate this response and test whether non-injured sensory neurons also alter FOXO3a expression,the temporal impact of chronic unilateral L4–6 spinal nerve transection on FOXO3a expression and nuclear localization in adult rat dorsal root ganglion neurons ipsilateral,contralateral or remote to injury relative to na?ve controls was *** na?ve neurons,high cytoplasmic and nuclear levels of FOXO3a colocalized with calcitonin gene related peptide,a marker of the nociceptive *** hour post-injury,an acute increase in nuclear FOXO3a in small size injured neurons occurred followed by a significant decrease after 1,2 and 4 days,with levels increasing toward pre-injury levels by 1 week post-injury.A more robust biphasic response to the injury was observed in uninjured neurons contralateral to and those remote to *** levels of FOXO3a peaked at 1 day,decreased by 4 days,then increased by 1 week post-injury,a response mirrored in C4 dorsal root ganglion neurons remote to *** altered expression contralateral and remote to injury supports that spinal nerve damage has broader systemic impacts,a response we recently reported for another stress transcription factor,Luman/*** early decreased expression and nuclear localization of FOXO3a in the injured neuron implicate these changes in the cell body response to injury that may be ***,the broader systemic changes support the existence of stress/inj

关键词： cell body response contralateral response dorsal root ganglion peripheral nerve injury plasticity sciatic nerve sensory neuron stress systemic transcription factor unilateral peripheral nerve injury

Novel vertically aligned nanocomposite of Bi_(2)WO_(6)-Co_(3)O_(4) with room-temperature multiferroic and anisotropic optical response

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Nano Research 2021年第12期14卷 4789-4794页

作者： Leigang Li Shikhar misra Xingyao Gao Juncheng Liu Han Wang Jijie Huang Bruce Zhang Ping Lu Haiyan Wang School of Materials Engineering Purdue UniversityWest LafayetteIndiana47907USA School of Electrical and Computer Engineering Purdue UniversityWest LafayetteIndiana47907USA Sandia National Laboratory AlbuquerqueNew Mexico87185USA

A new vertically aligned nanocomposite(VAN)structure based on two-dimensional(2D)layered oxides has been designed and self-assembled on both LaAlO_(3)(001)and SrTiO3(001)*** new VAN structure consists of epitaxially grown Co_(3)O_(4) nanopillars embedded in the Bi_(2)WO_(6) matrix with a unique 2D layered structure,as evidenced by the microstructural *** property measurements show that the new Bi_(2)WO_(6)-Co_(3)O_(4) VAN structure exhibits strong ferromagnetic and piezoelectric response at room temperature as well as anisotropic permittivity *** work demonstrates a new approach in processing multifunctional VANs structure based on the layered oxide systems towards future nonlinear optics,ferromagnets,and multiferroics.

关键词： vertically aligned nanocomposite(VAN) multiferroics Aurivillius oxides layered oxides Co_(3)O_(4) Bi_(2)WO_(6)