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Plant glycosylphosphatidylinositol anchored proteins at the plasma membrane-cell wall nexus

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Journal of Integrative Plant Biology 2018年第8期60卷 649-669页

作者： Trevor H.Yeats Antony Bacic Kim l.johnson School of Integrated Plant Sciences Section of Plant Biology Cornell University Robert W.Holley Center for Agriculture and Health USDA-ARS Cornell University Australian Research Council Centre of Excellence in Plant Cell Walls School of Bio Sciences University of Melbourne la Trobe Institute for Agriculture & Food Department of Animal Plant and Soil Sciences La Trobe University

Approximately 1% of plant proteins are predicted to be post-translationally modified with a glycosylphospha- tidylinositol （GPI） anchor that tethers the polypeptide to the outer leaflet of the plasma membrane. Whereas the synthesis and structure of GPI anchors is largely conserved across eukaryotes, the repertoire of functional domains present in the GPl-anchored proteome has diverged substantially. In plants, this includes a large fraction of the GPl-anchored proteome being further modified with plant-specific arabinogalactan （AG） O-glycans. The impor- tance of the GPl-anchored proteome to plant development is underscored by the fact that GPI biosynthetic null mutants exhibit embryo lethality. Mutations in genes encoding specific GPl-anchored proteins （GAPs） further supports their contribution to diverse biological processes, occurring at the interface of the plasma membrane and cell wall, including signaling, cell wall metabolism, cell wall polymer cross-linking, and plasmodesmatal transport. Here, we review the literature concerning plant GPl-anchored proteins, in the context of their potential to act as molecular hubs that mediate interactions between the plasma membrane and the cell wall, and their potential to transduce the signal into the protoplast and, thereby, activate signal transduction pathways.

关键词： GPI Plant glycosylphosphatidylinositol anchored proteins at the plasma membrane-cell wall nexus

Social-ecological correlates of fundamental movement skills in young children

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Journal of Sport and Health Science 2019年第2期8卷 122-129页

作者： Nan Zeng Susan l.johnson Richard E.Boles laura l.Bellows Department of Food Science and Human Nutrition Colorado State University Department of Pediatrics University of Colorado Anschutz Medical Campus

Purpose: To identify the social-ecological correlates associated with fundamental movement skills at the child, family, and environment levels in young ***: Preschool children from 4 Colorado Head Start/preschool centers were recruited from 2010 to 2012. Two hundred twenty-eight children(128 girls; age = 56.08 § 4.09 months; body mass index(BMI) z-score = 0.53 § 1.12(mean § SD); 42.1% Hispanic/latino) and 159 families were included in the final analysis. Children's perceived competence and fundamental movement skills were assessed via the Pictorial Scale of Perceived Competence and Social Acceptance and the Bruininks-Oseretsky Test of Motor Proficiency, 2 nd edition. Data on the number of children in the family, parent age, BMI, education, employment status, family income, perception of child coordination, and home physical activity environment were collected via a questionnaire. linear regressions adjusted for child BMI, age, sex, and school site were performed at each ***: Child perceived cognitive competence was positively associated with locomotor skills(p = 0.04; adjusted R2= 0.035) and object-control skills(p = 0.003; adjusted R2= 0.083) at the child level. Parent education, BMI, and perception of child coordination were positively associated with locomotor skills and explained 8.8% of variance, but only parent education was significant(p = 0.04) at the family level. In addition, physical environment was positively associated with locomotor skills(p = 0.02) and explained 5.5% of variance at the environment ***: Social-ecological correlates associated with young children's fundamental movement skills are multidimensional and differ according to skill category at the child, family, and environment levels.

关键词： Activity environment Fundamental movement skills Parent education Perceived cognitive competence Preschool children

Compact and robust supercontinuum generation and post-compression using multiple thin plates

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High Power laser Science and Engineering 2021年第4期9卷 234-241页

作者： Xinhua Xie Yunpei Deng Steven l.johnson Swiss FEl Paul Scherrer Institute5232 Villi genSwitzerland Institute for Quantum Electronics Physics DepartmentETH ZurichETH Zurich8093 ZurichSwitzerland

We report on compact and robust supercontinuum generation and post-compression using transmission of light through multiple thin solid plates at the Swiss FEl X-ray free-electron laser facility.A single stage consisting of three thin plates followed by a chirped mirror compressor achieves compression of initially 30-fs pulses with 800-nm center wavelength to sub-10-fs *** also demonstrate a two-stage implementation to compress the pulses further to sub-5-fs *** the two-stage setup,the generated supercontinuum includes wavelengths ranging from 500 to 1100 *** multiplate setup is compact,robust,and stable,which makes it ideal for applications at free-electron laser facilities such as pump-probe experiments and laser-arrival timing tools.

关键词： post-compression self-phase modulation supercontinuum generation

Insulin-like growth factor binding protein-5 influences pancreatic cancer cell growth

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World Journal of Gastroenterology 2009年第27期15卷 3355-3366页

作者： Sarah K johnson randy S Haun Department of Pathology Winthrop P. Rockefeller Cancer Institute University of Arkansas for Medical SciencesLittle Rock Arkansas AR 72205 United States

AIM： To investigate the functional significance of insulin-like growth factor binding protein-5 （IGFBP-5） overexpression in pancreatic cancer （PaC）.METHODS： The effects of IGFBP-5 on cell growth were assessed by stable transfection of BxPC-3 and PANC-1 cell lines and measuring cell number and DNA synthesis. Alterations in the cell cycle were assessed by flow cytometry and immunoblot analyses. Changes in cell survival and signal transduction were evaluated after mitogen and phosphatidylinositol activated protein kinase 3-kinase （PI3K） inhibitor treatment.RESUlTS： After serum deprivation, IGFBP-5 expression increased both cell number and DNA synthesis in BxPC-3 cells, but reduced cell number in PANC-1 cells. Consistent with this observation, cell cycle analysis of IGFBP-5-expressing cells revealed accelerated cell cycle progression in BxPC-3 and G2/M arrest of PANC-1 cells. Signal transduction analysis revealed that Akt activation was increased in BxPC-3, but reduced in PANC-1 cells that express IGFBP-5. Inhibition of PI3K with lY294002 suppressed extracellular signal-regulated kinase-1 and -2 （ERK1/2） activation in BxPC-3, but enhanced ERK1/2 activation in PANC-1 cells that express IGFBP-5. When MEK1/2 was blocked, Akt activation remained elevated in IGFBP-5 expressing PaC cells; however, inhibition of PI3K or MEK1/2 abrogated IGFBP-5-mediated cell survival.CONClUSION： These results indicate that IGFBP-5 expression affects the cell cycle and survival signal pathways and thus it may be an important mediator of PaC cell growth.

关键词： Insulin-like growth factor-binding protein 5 Extracellular signal-regulated mitogen activated protein kinases Cyclin-dependent kinase inhibitor p27 Pancreatic neoplasms

Fused-core particle technology in high-performance liquid chromatography: An overview

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Journal of Pharmaceutical Analysis 2013年第5期3卷 303-312页

作者： Joseph J.Kirkland Stephanie A.Schuster William l.johnson Barry E.Boyes Advanced Materials Technology Inc. Complex Carbohydrate Research Center

The advent of superficially porous particles （SPPs） for packed HPlC columns has changed the way that many practitioners have approached the problem of developing needed separations. The very high efficiency of such columns, combined with convenient operating conditions, modest back pressures and the ability to use conventional HPlC instruments has resulted in intense basic studies of SPP technology, and widespread applications in many sciences. This report contains an overview of the SPP technology first developed in 2006 by Advanced Materials Technology, Inc., for sub-3-11m particles, then expanded into a family of SPP products with different particle sizes, pore sizes and other physical parameters. This approach was designed so that each particle of the family could be optimized for separating a particular group of compounds, usually based on solute size.

关键词： Superfcially porous particles Fused-core particles Core–shell particles Peptides Proteins Drug separations

The proteome of distal nerves: implication in delayed repair and poor functional recovery

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Neural Regeneration Research 2022年第9期17卷 1998-2006页

作者： Song Guo Raymond M.Moore M.Cristine Charlesworth Kenneth l.johnson Robert J.Spinner Anthony J.Windebank Huan Wang Department of Neurologic Surgery Mayo ClinicRochesterMNUSA Biomedical Statistics and Informatics Mayo ClinicRochesterMNUSA Proteomics Core Mayo ClinicRochesterMNUSA Department of Neurology Mayo ClinicRochesterMNUSA

Chronic denervation is one of the key factors that affect nerve *** axotomy deteriorates the distal nerve stump,causes protein changes,and renders the microenvironment less permissive for *** of these factors/proteins have been individually *** better delineate the comprehensive protein expression profiles and identify proteins that contribute to or are associated with this detrimental effect,we carried out a proteomic analysis of the distal nerve using an established delayed rat sciatic nerve repair *** rats that received immediate repair after sciatic nerve transection served as control,whereas four rats in the experimental group(chronic denervation)had their sciatic nerve repaired after a 12-week *** the rats were sacrificed after 16 weeks to harvest the distal nerves for extracting ***-five micrograms of protein from each sample were fractionated in SDS-PAGE ***-MS/MS analysis was applied to the *** expression levels of nerves on the surgery side were compared to those on the contralateral *** protein with a P value of less than 0.05 and a fold change of 4 or higher was deemed differentially *** the differentially expressed proteins in both groups were further stratified according to the biological processes.A PubMed search was also conducted to identify the differentially expressed proteins that have been reported to be either beneficial or detrimental to nerve *** Pathway Analysis(IPA)software was used for pathway *** results showed that 709 differentially expressed proteins were identified in the delayed repair group,with a bigger proportion of immune and inflammatory process-related proteins and a smaller proportion of proteins related to axon regeneration and lipid metabolism in comparison to the control group where 478 differentially expressed proteins were *** experimental group also had more beneficial proteins that were d

关键词： chronic axotomy chronic denervation delayed repair distal nerve functional recovery nerve regeneration peripheral nerve prolonged denervation proteome sciatic nerve sciatic nerve transection

Beginning from the end:the presynaptic terminal as a pathomechanism hub in frontotemporal dementia and amyotrophic lateral sclerosis

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Neural Regeneration Research 2025年第11期20卷 3217-3218页

作者： laura Huggon Emma l.Clayton UK Dementia Research Institute at King’s College london LondonUK Department of Basic and Clinical Neuroscience Institute of Psychiatry Psychology&NeuroscienceKing’s College LondonLondonUK

Frontotemporal dementia and amyotrophic lateral sclerosis:Frontotemporal dementia(F T D)and amyo t rophic lateral sclerosis(AlS)are neurodegenerative diseases with significant overlapping *** these neurodegenerative diseases affect different neuronal populations(with FTD affecting neurons of the frontal and temporal lobes,and AlS affecting upper and lower motor neurons),these two diseases are complexly *** and AlS exist on a disease spectrum,with shared genetic causes,clinical presentations,and pathologies.

关键词： diseases amyotrophic sclerosis

Generation of millijoule-level sub-5 fs violet laser pulses

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High Power laser Science and Engineering 2024年第2期12卷 33-40页

作者： Xinhua Xie Yi Hung Yunpei Deng Adrian l.Cavalieri Andrius Baltuška Steven l.johnson SwissFEl Paul Scherrer InstituteVilligen PSISwitzerland Photonics Institute Technische Universität WienViennaAustria Institute of Applied Physics University of BernBernSwitzerland Institute for Quantum Electronics Physics DepartmentETH ZurichZurichSwitzerland

We demonstrate the generation,spectral broadening and post-compression of second harmonic pulses using a thin beta barium borate(BBO)crystal on a fused-silica substrate as the nonlinear interaction *** combining second harmonic generation in the BBO crystal with self-phase modulation in the fused-silica substrate,we efficiently generate millijoule-level broadband violet pulses from a single optical *** second harmonic spectrum covers a range from long wave ultraviolet(down to 310 nm)to visible(up to 550 nm)with a bandwidth of 65 ***,we compress the second harmonic beam to a duration of 4.8 fs with a pulse energy of 0.64 mJ(5 fs with a pulse energy of 1.05 mJ)using chirped *** all-solid free-space apparatus is compact,robust and pulse energy scalable,making it highly advantageous for generating intense second harmonic pulses from near-infrared femtosecond lasers in the sub-5 fs regime.

关键词： post-compression second harmonic generation self-phase modulation supercontinuum generation

Multiple sclerosis is at a checkpoint: advancing the program

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Neural Regeneration Research 2025年第3期20卷 811-812页

作者： Brandon C.Smith Jessica l.Williams Department of Neurosciences Lerner Research InstituteCleveland ClinicClevelandOHUSA Department of Biological Geologicaland Environmental SciencesCleveland State UniversityClevelandOHUSA

Multiple sclerosis(MS) is a chronic inflammatory and demyelinating disease of the central nervous system(CNS). Patients with MS experience sensory and motor function loss due to myelin and/or axon damage perpetuated b... 详细信息

关键词： Hauser sclerosis damage