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Molecular and Kinetic Characterization of the Fermentative Behavior of Saccharomyces cerevisiae Strains Isolated from North Patagonia

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Advances in Microbiology 2016年第9期6卷 733-744页

作者： Adriana Beatriz Simes ramona del carmen maturano Valentín Tassile Adriana Catalina Caballero Silvana María del Mónaco Facultad de Ciencias y Tecnología de los Alimentos Universidad Nacional del Comahue (UNCo) Villa Regina Argentina Grupo de Enología Instituto de Investigación y Desarrollo en Ingeniería de Procesos Biotecnología y Energías Alternativas (PROBIEN-Consejo Nacional de Investigaciones Científicas y Técnicas CONICET) Facultad de Ingeniería Universidad Nacional del Comahue (UNCo) Neuquén Argentina

At present, Argentina does not count with a production of indigenous yeast strains with suitable technological and oenological features to be used in the regional winery industry. Isolation and molecular characterization of these microorganisms and its fermentation attributes would be relevant to the sustainable development of the activity in the country and to recognize and preserve the biodiversity of the region. Eight strains isolated from grapes and musts from the North Patagonian region and genetically identified as Saccharomyces cerevisiae, were studied for their fermentation behavior, emphasizing in hexose transport through the plasma membrane, which is the limiting step of the process. Thus, sugar consumption profiles were analyzed in different media at laboratory scale, to be subsequently applied to the fermentation of natural musts. Three of the eight initial strains were selected, named NNM10, NIF8 and NMN16 according to their fermentation profiles. The expression of hexose transporters during fermentations revealed interesting differences in the response of each strain to sugar consumption, where transporters HXT2 and HXT5 showed significant changes in expression in Patagonian strains, which are normally associated to endurance to culture stress conditions. The results obtained by combining the characteristics studied, at molecular and physiological level, are extremely encouraging. Native strain NMN16, showed a high potential for application in local winemaking. Assays carried out on a pilot scale will determine the feasibility of applying this strain with promising technological features at industrial scale.

关键词： Fermentation Wine Saccharomyces cerevisiae Hexose Transporters

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Functional Rehabilitation of Disc Herniation Treated with Combined Therapy

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International Journal of Clinical Medicine 2023年第11期14卷 472-486页

作者： ramona delia Arsani Marius Stoica carmen Gherghel National University of Physical Education and Sports Bucharest Romania

Current researches reveal that the number of cases diagnosed with operated lumbar disc herniation is growing rapidly, which should draw an increased attention to the treatment used. The objective of this study is to perform a comparative analysis between the application of a classical physical-kinetic program and the application of a combined kinetic program. This research will be conducted on 20 subjects with ages, between 40 - 50 years old, in INEMRCM Bucharest, for 14 days - 1 hour a day. Each subject underwent an invasive surgery for lumbar disc herniation on the L5-S1 vertebrae. During the research, the 2 patients will be given a physical-kinetic program, adapted to the pathology of the operated lumbar disc herniation. The purpose of this research is to check the efficiency of each kinetic program. Furthermore, through the muscular and articular evaluation made in the initial phase and the final phase, it will be possible to discuss the comparative evolution of the established kinetic program and the functional gain obtained. In conclusion, the research shows that mechanized therapy results in faster functional rehabilitation and physical exercise substantially improves quality of life.

关键词： Lumbar Disc Herniation Rehabilitation Physical Therapy

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Silent diabetic nephropathy

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World Journal of Nephrology 2014年第1期3卷 6-15页

作者： Katia López-Revuelta Patricia Pea Galdo ramona Stanescu Leticia Parejo carmen Guerrero Elia Pérez-Fernández Unidad de Nefrología Hospital Universitario Fundación Alcorcón 28922 Madrid Spain Unidad de AnatomíaPatológica Hospital Universitario Fundación Alcorcón 28922 Madrid Spain Unidad de Investigación Hospital Universitario Fundación Alcorcón 28922 Madrid Spain

AIM: To examine the risk of renal events in patients with biopsy-proven diabetic nephropathy(DN) and its possible associated factors.METHODS: Clinical and histological data of 60 patients diagnosed with diabetic nephropathy were retrospectively collected. Patients with evidence or suspicion of other nephropathies were excluded from the study. The final event was defined as renal replacement therapy(RRT) initiation or progression of chronic kidney disease(CKD), according to the KDIGO 2012 definition of a decrease in CKD category and a decrease in GFR of 25% or more. RESULTS: A total of 45 patients with a follow-up of at least 3 mo were included. Most of the patients presented type 2 DM, with a median age of 58.3 years old. The time of evolution of DM was 9.6 ± 7.8 years, although in 13 patients, it was less than 5 years. A total of 62% of patients reached the final event in a mean period of 3.4 years(95%CI: 2.1-4.7), with 21 of them requiring dialysis. The factors that were independently associated with renal survival were estimated glomerular filtration rate(e GFR) at the time of biopsy, cardiovascular disease(CVD) history and Hb A1 c less than 7%. Therefore, for each 10 m L/min per 1.73 m2 reduction in e GFR, we obtained a DN progression risk of HR = 2(1.3-3.0)(P = 0.001); patients with CVD were at greater risk for DN progression(HR = 2.8, 1.1-7.1, P = 0.032), and CKD patients with Hb A1 c < 7% demonstrated greater renal risk than patients with Hb A1 c ≥ 7%, with an HR of 2.9(1.0-8.4)(P = 0.054).CONCLUSION: A past history of CVD is a risk factor for DN progression. Levels of Hb A1 c less than 7% could favor an e GFR decrease in these patients.

关键词：糖尿病肾病患者治疗方法

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