检索结果-南通市图书馆

Impact of high dose vitamin c on platelet function

World Journal of critical care Medicine 2017年第1期6卷 37-47页

作者： Bassem M Mohammed Kimberly W Sanford Bernard J Fisher Erika J Martin Daniel contaifer Jr Urszula Osinska Warncke Dayanjan S Wijesinghe charles E chalfant Donald F Brophy Alpha A Fowler Ⅲ ramesh Natarajan Department of clinical Pharmacy Faculty of Pharmacy Cairo University Department of Pharmacotherapy and Outcomes Science Virginia Commonwealth University Department of Pathology Virginia Commonwealth University Division of Pulmonary Disease and critical care Medicine De-partment of Internal Medicine Virginia Commonwealth University Research and Development Hunter Holmes Mc Guire Veterans Administration Medical Center the Department of Biochemistry and Molecular Biology the VCU Massey Cancer Center the VCU Institute of Molecular Medicine and the VCU Johnson Center for Critical Care and Pulmonary Research Virginia Commonwealth University

AIM To examine the effect of high doses of vitamin c(Vitc) on ex vivo human platelets(PLTs).METHODS Platelet concentrates collected for therapeutic or prophylactic transfusions were exposed to:(1) normal saline(control);(2) 0.3 mmol/L Vitc(Lo Vitc); or(3) 3 mmol/L Vitc(Hi Vitc, final concentrations) and stored appropriately. The Vit c additive was preservative-free buffered ascorbic acid in water, pH 5.5 to 7.0, adjusted with sodium bicarbonate and sodium hydroxide. The doses of Vit c used here correspond to plasma Vit c levels reported in recently completed clinical trials. Prior to supplementation, a baseline sample was collected for analysis. PLTs were sampled again on days 2, 5 and 8 and assayed for changes in PLT function by: Thromboelastography(TEG), for changes in viscoelastic properties; aggregometry, for PLT aggregation and adenosine triphosphate(ATP) secretion in response to collagen or adenosine diphosphate(ADP); and flow cytometry, for changes in expression of cD-31, cD41 a, cD62 p and cD63. In addition, PLT intracellular Vit c content was measured using a fluorimetric assay for ascorbic acid and PLT poor plasma was used for plasma coagulation tests [prothrombin time(PT), partial thrombplastin time(PTT), functional fibrinogen] and Lipidomics analysis(UPLc ESI-MS/MS).RESULTS Vitc supplementation significantly increased PLTs intracellular ascorbic acid levels from 1.2 mmol/L at baseline to 3.2 mmol/L(Lo Vitc) and 15.7 mmol/L(Hi Vitc, P c supplementation did not significantly change PT and PTT values, or functional fibrinogen levels over the 8 d exposure period(P > 0.05). PLT function assayed by TEG, aggregometry and flow cytometry was not significantly altered by Lo or Hi Vitc for up to 5 d. However, PLTs exposed to 3 mmol/L Vitc for 8 d demonstrated significantly increased R and K times by TEG and a decrease in the α-angle(P ciated with the Hi Vitc compared to both baseline and d

关键词： Platelet function Thromboelastography Flow cytometry Platelet lipidomics Vitamin c

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

clinical, cellular, microscopic, and ultrastructural studies of a case of fibrogenesis imperfecta ossium

引用

Bone Research 2017年第2期5卷 123-136页

作者： Melissa L Barron Mark S Rybchyn Sutharshani ramesh Rebecca S Mason S Fiona Bonar Paul Stalley Sundeep Khosla Bernie Hudson christopher Arthur Edward Kim Roderick J clifton-Bligh Phillip B clifton-Bligh Department of Physiology School of Medical Sciences Bosch Institute University of Sydney Sydney 2006 New South Wales Australia Douglas HanlyMoir Pathology Macquarie Park 2113 New South Wales Australia Department of Orthopaedics Royal Prince Alfred HospitalCamperdown 2050 New South Wales Australia Department of Endocrinology Mayo Clinic Rochester 55905 MN USA Department of Microbiology Royal North Shore Hospital St Leonards 2065 New South Wales Australia Department ofMicrobiology Royal North Shore Hospital St Leonards 2065 New South Wales Australia Department of Haematology Royal North Shore Hospital St Leonards 2065 New South Wales Australia Department of Endocrinology Royal North Shore Hospital St Leonards 2065 New SouthWales Australia and Faculty of Medicine University of Sydney Sydney 2006 New South Wales Australia

Fibrogenesis imperfecta ossium is a rare disorder of bone usually characterized by marked osteopenia and associated with variable osteoporosis and osteosclerosis, changing over time. Histological examination shows that newly formed collagen is abnormal, lacking birefringence when examined by polarized light. The case presented demonstrates these features and, in addition, a previously undocumented finding of a persistent marked reduction of the serum c3 and c4. Osteoblasts established in culture from a bone biopsy showed abnormal morphology on electron microscopy and increased proliferation when cultured with benzoylbenzoyl-ATP and 1,25-dihydroxyvitamin D, contrasting with findings in normal osteoblasts in culture. A gene microarray study showed marked upregulation of the messenger RNA(mRNA) for G-protein-coupled receptor 128(GPR 128), an orphan receptor of unknown function and also of osteoprotegerin in the patient's osteoblasts in culture. When normal osteoblasts were cultured with the patient's serum, there was marked upregulation of the mRNA for aquaporin 1. A single pathogenetic factor to account for the features of this disorder has not been defined, but the unique findings described here may facilitate more definitive investigation of the abnormal bone cell function.

关键词： ultrastructural cellular clinical

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

利用持续培养系统研究不同脂肪酸组分的脂类添加对瘤胃微生物菌群的影响

引用

中国畜牧兽医 2011年第12期38卷 159-159页

作者： ramesh B P 姜雅慧

本研究利用单外流持续发酵系统评价日粮中添加含不同脂肪酸成分的脂类对瘤胃中特定微生物DNA浓度的影响。试验系统有4个发酵罐,利用4×4拉丁方设计,试验共进行4期,每期10d。每个发酵罐每天投料3次,每天投料45g(DM基础)。处理日粮分别为... 详细信息

本研究利用单外流持续发酵系统评价日粮中添加含不同脂肪酸成分的脂类对瘤胃中特定微生物DNA浓度的影响。试验系统有4个发酵罐,利用4×4拉丁方设计,试验共进行4期,每期10d。每个发酵罐每天投料3次,每天投料45g(DM基础)。处理日粮分别为:①对照组(cON),②对照组日粮中添加动物脂类(SAT),③对照组日粮中添加豆油(SBO),④对照组日粮中添加鱼油(FO)。添加脂类的量占日粮添加量的3%。结果表明,添加脂类不会影响总挥发性脂肪酸和乙酸产量(P>0.05),FO处理组瘤胃液中丙酸、异丁酸、戊酸和异戊酸的含量高于其他处理组(Pc18∶1(异油酸)含量有所增加(Pc18∶0的浓度较其他处理组低(Pca和Butyrivibrioproteoclastic细菌DNA含量一致(P>0.05),FO处理组中Butyrivibriof ibrisolvens和Ruminococcus albus的DNA含量低于其他处理组(P<0.05),而其他处理组其含量一致。FO处理组中Selenomonas ruminantium菌DNA浓度最高(P<0.05),据以上结论可知,豆油对本试验中所检测细菌的DNA含量无影响,添加鱼油会增加异油酸在瘤胃中的积累,部分原因可能与鱼油会影响瘤胃中***、***和***细菌的生长有关。

关键词：脂类脂肪酸细菌发酵罐

来源：

维普期刊数据库博看期刊

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

含有不同脂肪酸组分的持续培养系统中添加脂类对瘤胃细菌的影响

引用

中国畜牧兽医 2011年第10期38卷 159-159页

作者： ramesh B P 姜雅慧

本研究利用单外流持续培养系统研究日粮中添加含不同脂肪酸组分的脂类对瘤胃中目的细菌DNA浓度的影响。试验所用4个发酵罐,采用4×4拉丁方设计,共进行4期试验,每期10d。试验日粮:①对照组(cON);②SAT组,对照组日粮添加动物脂肪;③SBO组... 详细信息

本研究利用单外流持续培养系统研究日粮中添加含不同脂肪酸组分的脂类对瘤胃中目的细菌DNA浓度的影响。试验所用4个发酵罐,采用4×4拉丁方设计,共进行4期试验,每期10d。试验日粮:①对照组(cON);②SAT组,对照组日粮添加动物脂肪;③SBO组,对照组日粮添加豆油;④FO组,对照组日粮添加鱼油。脂类的添加量为日投料量的3%DM。添加脂类不会影响发酵液中的总挥发性脂肪酸和乙酸的产量(P>0.05)。FO组发酵液中丙酸、异丁酸、戊酸和异戊酸的含量较其他处理组高(Pc18:1(VA)的含量(Pc18:0的含量低于其他处理组(Pca和Butyrivibrio proteoclasticus的数量无显著差异(P>0.05)。FO处理组中Butyrivibriofibrisolvens和Ruminococcus albus的数量最低(P0.05)。FO处理组中Selenomonas ruminantium的DNA浓度最高(Pca、Butyrivibrio proteoclasticus和Sel-enomonas ruminantium的生长有关。

关键词：脂类脂肪酸细菌发酵罐

来源：

维普期刊数据库博看期刊

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

含有不同脂肪酸组分的持续培养系统中添加脂类对瘤胃细菌的影响

引用

中国畜牧兽医 2011年第11期38卷 131-131页

作者： ramesh B P 姜雅慧

本研究利用单外流持续培养系统研究日粮中添加含不同脂肪酸组分的脂类对瘤胃中目的细菌DNA浓度的影响。试验用4个发酵罐,采用4×4拉丁方设计,共进行4期试验,每期10d。试验日粮：①对照组（cON）;②SAT组,对照组日粮添加动物脂肪;③SBO组... 详细信息

本研究利用单外流持续培养系统研究日粮中添加含不同脂肪酸组分的脂类对瘤胃中目的细菌DNA浓度的影响。试验用4个发酵罐,采用4×4拉丁方设计,共进行4期试验,每期10d。试验日粮：①对照组（cON）;②SAT组,对照组日粮添加动物脂肪;③SBO组,对照组日粮添加豆油;④FO组,对照组日粮添加鱼油。脂类的添加量为日投料量的3%（干物质基础）。结果表明,添加脂类不会影响发酵液中的总挥发性脂肪酸和乙酸的产量（P〉0.05）;FO组发酵液中丙酸、异丁酸、戊酸和异戊酸的含量较其他处理组高（P〈0.05）;SBO和FO处理组溢流液中的t-11c18∶1（VA）的含量显著升高（P〈0.05）,FO组的VA含量最高;而FO处理组溢流液中c18∶0的含量显著低于其他处理组（P〈0.05）。各处理组发酵罐内Anaerovibrio lipolytica和Butyrivibrio proteoclasticus的数量无显著差异（P〉0.05）;FO处理组中Butyrivibrio fibrisolvens和Ruminococcusalbus的数量最低,其他几个处理组间无显著差异（P〉0.05）;FO处理组中Selenomonas ruminantium的DNA浓度最高。总之,豆油对待测细菌的DNA浓度无显著影响,在鱼油处理组中异油酸在瘤胃中出现积累可能部分因鱼油影响Anaerovibrio lipolytica、Butyrivibrio proteoclasticus和Selenomonas ruminantium的生长。

关键词：脂类脂肪酸细菌发酵罐

来源：

维普期刊数据库博看期刊

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Endoscopic closure of a gastrocolic fistula using the over-the-scope-clip-system

引用

World Journal of Gastrointestinal Endoscopy 2013年第8期5卷 402-406页

作者： Klaus Mnkemüller Shajan Peter Basem Alkurdi Jayapal ramesh Daniel Popa c Mel Wilcox Division of Gastroen-terology and Hepatology Basil Hirschowitz Endoscopic Center of Excellence University of Alabama at Birmingham

Gastrointestinal (GI) defects such as fistulas and leaks can be potentially closed endoscopically using hemo-clips and loops. However, hemoclips may not allow for closure of large defects and they do not exert enough tensile force to keep fibrotic defects larger than 5 mm approximated. Herein we present a case of successful endoscopic closure of a gastrocolic fistula in a severely malnourished patient with complex post-surgical upper GI anatomy. We strongly believe that this device is a major breakthrough for the management of various types of discontinuity defects or fistulas. In addition, we show the usefulness of placing a direct jejunostomy using the double balloon enteroscopy (DBE) technique during the same procedure. The concept of providing direct jejunal feedings while allowing for upper gas-trointestinal bowel rest to promote the healing of the minimally invasive endoscopic operation is novel. Thus, our case is unique and exemplifies the utility of mini-mally invasive endoscopic endoluminal surgery.

关键词： Over-the-scope-clip Bear claw Fistula Endoscopic closure Gastrocolic fistula Over the scope clip clip

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

New-onset diabetes mellitus after kidney transplantation:current status and future directions

引用

世界糖尿病杂志：英文版（电子版） 2015年第3期6卷 445-455页

作者： Sneha Palepu G V ramesh Prasad Division of Nephrology University of TorontoSt.Michael’s Hospital

A diagnosis of new-onset diabetes after transplantation(NODAT) carries with it a threat to the renal allograft,as well as the same short-and long-term implications of type 2 diabetes seen in the general *** usually occurs early after transplantation,and is usually diagnosed according to general population ***-modifiable risk factors for NODAT include advancing age,African American,Hispanic,or South Asian ethnicity,genetic background,a positive family history for diabetes mellitus,polycystic kidney disease,and previously diagnosed glucose *** risk factors for NODAT include obesity and the metabolic syndrome,hepatitis c virus and cytomegalovirus infection,corticosteroids,calcineurin inhibitor drugs(especially tacrolimus),and *** affects graft and patient survival,and increases the incidence of post-transplant cardiovascular *** incidence and impact of NODAT can be minimized through pre-and post-transplant screening to identify patients at higher risk,including by oral glucose tolerance tests,as well as multi-disciplinary care,lifestyle modification,and the use of modified immunosuppressive regimens coupled with glucose-lowering therapies including oral hypoglycemic agents and *** NODAT is a major cause of post-transplant morbidity and mortality,measures to reduce its incidence and impact have the potential to greatly improve overall transplant success.

关键词： cyclosporine Graft Kidney New-onsetdiabetes Tacrolimus Transplantation

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Symmetry-aware recursive image similarity exploration for materials microscopy

引用

npj computational Materials 2021年第1期7卷 1508-1521页

作者： Tri N.M.Nguyen Yichen Guo Shuyu Qin Kylie S.Frew Ruijuan Xu Joshua c.Agar Department of Materials Science and Engineering Lehigh UniversityBethlehemPA 18015USA Department of computer Science and Engineering Lehigh UniversityBethlehemPA 18015USA Department of Mechanical Engineering Lehigh UniversityBethlehemPA 18015USA Department of Applied Physics Stanford UniversityStanfordCA 94305USA

In pursuit of scientific discovery,vast collections of unstructured structural and functional images are acquired;however,only an infinitesimally small fraction of this data is rigorously analyzed,with an even smaller fraction ever being *** method to accelerate scientific discovery is to extract more insight from costly scientific experiments already ***,data from scientific experiments tend only to be accessible by the originator who knows the experiments and ***,there are no robust methods to search unstructured databases of images to deduce correlations and ***,we develop a machine learning approach to create image similarity projections to search unstructured image *** improve these projections,we develop and train a model to include symmetry-aware *** an exemplar,we use a set of 25,133 piezoresponse force microscopy images collected on diverse materials systems over five *** demonstrate how this tool can be used for interactive recursive image searching and exploration,highlighting structural similarities at various length *** tool justifies continued investment in federated scientific databases with standardized metadata schemas where the combination of filtering and recursive interactive searching can uncover synthesis-structure-property *** provide a customizable open-source package(https://***/m3-learning/Recursive_Symmetry_Aware_Materials_Microstructure_Explorer)of this interactive tool for researchers to use with their data.

关键词： interactive image similarity

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Exhibition of Bleaching Resistance Via Adaptive Bleaching Pattern by coral Reefs of the Gulf of Mannar during Massive Bleaching Event 2019

引用

Journal of Marine Science 2020年第2期2卷 1-5页

作者： cH.ramesh S.Koushik T.Shunmugaraj M.V.Ramana Murthy National centre for coastal Research(NccR) Ministry of Earth SciencesNCCR Field OfficeMandapam-623519Tamil NaduIndia National centre for coastal Research PallikaranaiChennai-600100Tamil NaduIndia

coral reefs in the Gulf of Mannar(GoM)and Palk Bay regions are facing potential threats from climate change,sedimentation and anthropogenic ***,coral reefs in these two regions are highly damaged due to frequent bleaching events caused by increased sea surface temperature(SST)recorded over the past two ***,during February to May 2019,mass bleaching event of corals was documented again in GoM and Palk Bay regions due to increased sea surface temperatures ranged between 32°c to 36°*** of completely bleached corals,some coral colonies of the same bleached coral species have displayed adaptive bleaching pattern(ABP)for *** incidence is displayed by the symbiotic zooxanthellae to protect coral polyps from environmental stress such as elevated *** observations infer that coral colonies with preferential bleaching pattern ability would serve as environmental stress resisting coral colonies which can be used for restoration activities and *** studies are needed to explain the potential mechanisms or specific environmental drivers responsible for ABP.

关键词： coral bleaching Adaptive bleaching pattern Gulf of Mannar Palk Bay Temperature

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：