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Characterization of clarithromycin resistance in Malaysian isolates of Helicobacter pylori

World Journal of Gastroenterology 2009年第25期15卷 3161-3165页

作者： Norazah Ahmad Wan Rasinah Zakaria Sheikh Anwar Abdullah Ramelah Mohamed Bacteriology Unit Institute for Medical Research Department of Internal Medicine Universiti Kebangsaan Malaysia Medical Centre Universiti Kebangsaan Malaysia Molecular Biological Institute Universiti Kebangsaan Malaysia Medical Centre

AIM:To characterize the types of mutations present in the 23S rRNA genes of Malaysian isolates of clarithromycin-resistant Helicobacter pylori (H pylori).METHODS: Clarithromycin susceptibility of H pylori isolates was determined by E test. Analyses for point mutations in the domain V of 23S rRNA genes in clarithromycin-resistant and-sensitive strains were performed by sequence analysis of amplified polymerase chain reaction products. Restriction fragment length polymorphism was performed using Bsa-RESULTS: Of 187 isolates from 120 patients, four were resistant to clarithromycin, while 183 were sensitive. The MIC of the resistant strains ranged from 1.5 to 24 μg/mL. Two isolates had an A2142G mutation and another two had A2143G mutations. A T2182C mutation was detected in two out of four clarithromycin-resistant isolates and in 13 of 14 clarithromycin-sensitive isolates. Restriction enzyme analyses with Bsa-CONCLUSION: Clarithromycin resistance is an un-common occurrence among Malaysian isolates of H pylori strains and the mutations A2142G and A2143G detected were associated with low-level resistance.

关键词：幽门螺杆菌克拉霉素耐药菌株马来西亚限制性片段长度多态性 rRNA基因聚合酶链反应突变检测

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Ethnicity association of Helicobacter pylori virulence genotype and metronidazole susceptibility

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World Journal of Gastroenterology 2013年第8期19卷 1283-1291页

作者： Hanafiah Alfizah Awang Hamat Rukman Ahmad Norazah Razlan Hamizah mohamed ramelah Department of Medical Medical Microbiology and Immunology Faculty of MedicineUniversiti Kebangsaan Malaysia Medical Centre56000 CherasKuala LumpurMalaysia Department of Medical Microbiology and Parasitology Faculty of Medicine and Health SciencesUniversiti Putra Malaysia43400 SerdangSelangorMalaysia Bacteriology Unit Institute for Medical ResearchJalan Pahang50588 Kuala LumpurMalaysia Department of Medicine Faculty of MedicineUniversiti Kebangsaan Malaysia Medical Centre56000 CherasKuala LumpurMalaysia Centre for Innovative Collaboration Universiti Kebangsaan Malaysia43600 BangiSelangorMalaysia

AIM:To characterise the cag pathogenicity island in Helicobacter pylori(H.pylori) isolates by analysing the strains' vacA alleles and metronidazole susceptibilities in light of patient ethnicity and clinical outcome.METHODS:Ninety-five H.pylori clinical isolates obtained from patients with dyspepsia living in Malaysia were analysed in this study.Six genes in the cagPAI region(cagE,cagM,cagT,cag13,cag10 and cag67) andvacA alleles of theH.pylori isolates were identified by polymerase chain reaction.The isolates' metronidazole susceptibility was also determined using the E-test method,and the resistant gene was characterised by sequencing.RESULTS:More than 90% of the tested isolates had at least one gene in the cagPAI region,and cag67 was predominantly detected in the strains isolated from the Chinese patients,compared with the Malay and Indian patients(P < 0.0001).The majority of the isolates(88%) exhibited partial deletion(rearrangement) in the cagPAI region,with nineteen different patterns observed.Strains with intact or deleted cagPAI regions were detected in 3.2% and 8.4% of isolates,respectively.The prevalence of vacA s1m1 was significantly higher in the Malay and Indian isolates,whereas the isolates from the Chinese patients were predominantly genotyped as vacA s1m2(P = 0.018).Additionally,the isolates from the Chinese patients were more sensitive to metronidazole than the isolates from the Malay and Indian patients(P = 0.047).Although we attempted to relate the cagPAI genotypes,vacA alleles and metronidazole susceptibilities to disease outcome,no association was observed.The vacA alleles were distributed evenly among the strains with intact,partially deleted or deleted cagPAI regions.Interestingly,the strains exhibiting an intact cagPAI region were sensitive to metronidazole,whereas the strains with a deleted cagPAI were more resistant.CONCLUSION:Successful colonisation by different H.pylori genotypes is dependent on the host's genetic makeup and may play an important role in the clinical outcome.

关键词： Helicobacter pylori cag pathogenicity island vacA alleles Metronidazole susceptibility

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马拉西亚地区幽门螺杆菌菌株对克拉霉素耐药情况

马拉西亚地区幽门螺杆菌菌株对克拉霉素耐药情况

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贵州省中西医结合学会2011年消化系病学术交流会暨消化...

作者： Norazah Ahmad Wan Rasinah Zakaria Sheikh Anwar Abdullah Ramelah Mohamed 王昊周力贵阳医学院

目的:研究在马来西亚地区幽门螺杆菌菌株对克拉霉素耐药情况与23SrRNA基因突变的关系。方法:通过E test方法测定幽门螺杆菌菌株对克拉霉素的敏感性,通过对克拉霉素耐药菌株和克拉霉素敏感菌株的23S rRNA基因V区的点突变进行分析聚合酶... 详细信息

目的:研究在马来西亚地区幽门螺杆菌菌株对克拉霉素耐药情况与23SrRNA基因突变的关系。方法:通过E test方法测定幽门螺杆菌菌株对克拉霉素的敏感性,通过对克拉霉素耐药菌株和克拉霉素敏感菌株的23S rRNA基因V区的点突变进行分析聚合酶链反应产物的序列分析扩增。应用限制性内切酶(Mbo II,Bsa I)酶切位点检测相对应的克拉霉素耐药菌株的突变已达到对限制性片段长度多态性分析。结果:120例患者的187个菌株中,有4株对克拉霉素耐药,183株对克拉霉素敏感。该耐药菌株的MIC(最低抑菌浓度)范围从1.5到24ug/ml.两个菌株中一个是A2142G突变,另两个是A2143G突变。4个克拉霉素耐药菌株中2个被检测到T2182C突变,14个克拉霉素敏感菌株中13个被检测到T2182C突变。限制性内切酶(Bsa I、MboII)可检测到突变。结论:在马来西亚地区幽门螺杆菌菌株对克拉霉素耐药的发生比较罕见,A2142G和A2143G突变存在低水平耐药。

关键词：克拉霉素耐药幽门螺杆菌 23srRNA突变限制性片段长度多态性

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