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Analysis of Rna-Dependent Rna Polymerase Sequence of Infectious Flacherie Virus Isolated in China and Its Expression in BmN Cells

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Agricultural Sciences in China 2009年第7期8卷 872-879页

作者： LI Ming-qian LU Qi-neng WU Xiao-Feng MAN na-na CHEN Xiao-xue JIN Wei LU Xing-meng Laboratory of lnvertebrate Pathology College of Animal Sciences Zhejiang University Hangzhou 310029 P.R.China

Full gene sequence of Rna-dependent Rna polymerase （RdRp） from Bombyx mori infectious flacherie virus isolated in Zhejiang Province, China （Zhejiang01/CHN/2002） was cloned. The sequence was 1 920 nucleotides in length coding 639 amino acid residues. Sequences comparison of RdRp showed Zhejiang01/CHN/2002 was 99.7% nucleotide sequence and 99.1% amino acids sequence homology with Japanese strain. The RdRp sequence was aligned with 8 representative picorna（-like） viruses and 8 highly conserved regions were detected. The result indicated their relevance function. Phylogenetic tree of 14 picorna（-like） viruses which RdRp presumed protein sequences revealed that the viruses from Iflavirus genus formed an independent clade. The RdRp was successfully expressed in BmN cells using Bac-to-Bac expression system.

关键词： infectious flacherie virus Rna-dependent Rna polymerase phylogenetic analysis BmN cells expression

Effects of soil conservation practices on soil erosion and the size selectivity of eroded sediment on cultivated slopes

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Journal of Mountain Science 2021年第5期18卷 1222-1234页

作者： XU Lu ZHANG Dan PROSHAD ram CHEN Yu-lan HUANG Tian-fang UGURLU Aysenur Institute of Mountain Hazards and Environment Chinese Academy of SciencesChengdu 610041China University of Chinese Academy of Sciences Beijing 100049China Liangshan Company of Sichuan Provincial Tobacco Co. Xichang 615000China Chengdu Institute of Biology Chinese Academy of SciencesChengdu 610041China

Soil conservation practices can greatly affect the soil erosion process,but limited information is available about its influence on the particle size distribution(PSD)of eroded sediment,especially under natural *** this study,the runoff,sediment yields,and effective/ultimate PSD were measured under two conventional tillage practices,downhill ridge tillage(DT)and plat tillage(PT)and three soil conservation practices,contour ridge tillage(CT),mulching with downhill ridge tillage(MDT),and mulching with contour ridge tillage(MCT)during 21 natural rainfall events in the lower Jinsha *** results showed that(1)soil conservation practices had a significant effect on soil *** conventional tillage of DT caused highest runoff depth(0.58 to 29.13 mm)and sediment yield(0.01 to 3.19 t hm^(-2)).Compared with DT,the annual runoff depths and sediment yields of CT,MDT and MCT decreased by 12.24%-49.75%and 40.79%-88.30%,respectively.(2)Soil conservation practices can reduce the decomposition of aggregates in *** ratios of effective and ultimate particle size(E/U)of siltand sand-sized particles of DT and PT plots were close to 1,indicating that they were transported as primary particles,however,values lower/greater than 1 subject to CT,MDT and MCT plots indicated they were transported as *** ratios of E/U of claysized particles were all less than 1 independently of tillage practices.(3)The sediments of soil conservation practices were more selective than those of conventional tillage *** CT,MDT and MCT plots,the average enrichment ratios(ERs)of clay,silt and sand were 1.99,1.93 and 0.42,respectively,with enrichment of clay and silt and depletion of sand in ***,the compositions of the eroded sediments of DT and PT plots were similar to that of the original *** findings support the use of both effective and ultimate particle size distributions for studying the size selectivity of eroded sediment,and provide a scien

关键词： natural rainfall Runoff and sediment yield Soil particle size distribution Enrichment ratio Purple soil

The role of physical properties in explosive welding of copper to stainless steel

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Defence Technology（防务技术） 2023年第4期22卷 88-98页

作者： G.H.S.F.L.Carvalho I.Galvao R.Mendes R.M.leal A.B.Moreira A.Loureiro Univ Coimbra CEMMPREDepartamento de Engenharia Mec^anicaRua Luís Reis Santos3030-788CoimbraPortugal Department of Industrial Engineering University of FirenzeVia di Santa Marta 350139FirenzeItaly ISEL Departamento de Engenharia Mec^anicaInstituto Politecnico de LisboaRua Conselheiro Emídio Navarro 11959-007LisboaPortugal Univ Coimbra ADAILEDAPDepartamento de Engenharia Mec^anicaRua Luís Reis Santos3030-788CoimbraPortugal LIDA-ESAD.CR Instituto Politecnico de LeiriaRua Isidoro Inacio Alves de Carvalho2500-321Caldas da RainhaPortugal Univ.Porto LAETA/INEGIDept.Eng.Metalúrgica e de MateriaisR.Dr.Roberto Frias4200-465PortoPortugal

This paper investigates the effects of the physical properties on the microstructure and weldability of explosive welding by joining two metals with a significant contrast in thermophysical properties:stainless steel and *** welds between stainless steel and copper were obtained,and the interfacial morphology was wavy,regardless of the position of the *** weldability of dissimilar pairs was found to be more dependent on the relationship between the physical properties of the base materials than on the absolute value of the material *** there is a significant difference in thermal conductivity between the flyer and the base plate,together with a material with a low melting temperature,the weldability of the pair is often *** relative position of the plates affects the interfacial microstructure even when similar morphologies are *** the metallic pairs studied,the wave size was bigger for the configuration in which the ratio between the density of the flyer and the density of the base plate is *** same phenomenon was observed for the impedance:bigger waves were found for a smaller ratio between the impedance of the flyer and the impedance of the base plate.

关键词： Explosive welding Solid-state Copper-stainless steel Dissimilar Interface morphology Weldability

The Deep Thioredoxome in Chlamydomonas reinhardtii： New Insights into Redox Regulation

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Molecular Plant 2017年第8期10卷 1107-1125页

作者： Maria Esther perez-perez Adeline Mauries Alexandre Maes Nicolas J. Tourasse Marion Hamon Stephane D. Lemaire Christophe H. Marchand Institut de Biologie Physico-Chimique UMR8226 CNRS Sorbonne Universit6s UPMC Univ Paris 06 13 rue Pierre et Marie Curie 75005 Paris France Institut de Biologie Physico-Chimique Plateforme de Proteomique FRC550 CNRS 13 rue Pierre et Marie Curie 75005 Paris France Instituto de Bioquimica Vegetal y Fotosintesis Consejo Superior de Investigaciones Cientificas (CSIC)-Universidad de Sevilla Seville Spain ARna Laboratory INSERM U1212 CNRS UMR5320 Universite Bordeaux 2 Bordeaux France

Thiol-based redox post-translational modifications have emerged as important mechanisms of signaling and regulation in all organisms, and thioredoxin plays a key role by controlling the thiol-disulfide status of target proteins. Recent redox proteomic studies revealed hundreds of proteins regulated by glutathio- nylation and nitrosylation in the unicellular green alga Chlamydomonas reinhardtii, while much less is known about the thioredoxin interactome in this organism. By combining qualitative and quantitative proteomic analyses, we have comprehensively investigated the Chlamydomonas thioredoxome and 1188 targets have been identified. They participate in a wide range of metabolic pathways and cellular pro- cesses. This study broadens not only the redox regulation to new enzymes involved in well-known thiore- doxin-regulated metabolic pathways but also sheds light on cellular processes for which data supporting redox regulation are scarce （aromatic amino acid biosynthesis, nuclear transport, etc）. Moreover, we char- acterized 1052 thioredoxin-dependent regulatory sites and showed that these data constitute a valuable resource for future functional studies in Chlamydomonas. By comparing this thioredoxome with proteomic data for glutathionylation and nitrosylation at the protein and cysteine levels, this work confirms the existence of a complex redox regulation network in Chlamydomonas and provides evidence of a tremendous selectivity of redox post-translational modifications for specific cysteine residues.

关键词： Chlamydomonas reinhardtii disulfide bond isotope-coded affinity tag redox proteomics redox regulation thioredoxin targets

关于提高慢性气道疾病患者吸入制剂用药依从性的实践与探索

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中国医院药学杂志 2024年第2期44卷 195-199,220页

作者：于小杰杨文明赵艳敏张伟威王娜秦皇岛市第一医院药学部河北秦皇岛066000

目的:探讨慢性气道疾病患者出院后使用吸入制剂依从性的影响因素及回访周期优化,探索提高患者用药依从性的方法。方法:收集秦皇岛市第一医院呼吸与危重症医学科2019年6月至2021年6月出院的医嘱长期使用吸入制剂的慢性气道疾病患者信息,... 详细信息

目的:探讨慢性气道疾病患者出院后使用吸入制剂依从性的影响因素及回访周期优化,探索提高患者用药依从性的方法。方法:收集秦皇岛市第一医院呼吸与危重症医学科2019年6月至2021年6月出院的医嘱长期使用吸入制剂的慢性气道疾病患者信息,通过调查问卷考察患者出院后2周、1个月、2个月、6个月、12个月、18个月使用吸入制剂依从性情况。结果:该研究纳入患者整体依从性较差,logistic回归分析显示文化程度低、无法正确操作装置、同时使用吸入制剂数量多是依从性差的危险因素,事后多重比较分析显示,临床药师分别于患者出院后2周、1个月、2个月、6个月、12个月、18个月持续电话随访可逐步提高用药依从性,12个月和18个月两组间依从性得分相似,可将随访至出院后12个月作为一个回访周期。结论:随访12个月可有效提高患者用药依从性,临床药师在随访过程中应重点关注涉及危险因素人群,加强此类患者的用药教育、用药提醒。

关键词：吸入制剂用药依从性影响因素回访周期用药教育方式

Forecasting promising technology using analysis of patent information:Focused on next generation mobile communications

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Journal of Central South University 2014年第11期21卷 4303-4310页

作者： CHOI Seung-Wook YOU Yen-Yoo na Kwan-Sik Department of Knowledge Service & Consulting Hansung UniversitySeoul Korea Department of Management Information Systems Seowon UniversityCheongju Korea

In order to forecast promising technologies in the field of next generation mobile communication, various patent indicators were analyzed such as citation per patent, patent family information, patent share, increase rate, and patent activity. These indicators were quantified into several indexes and then integrated into an evaluation score to provide promising technologies. As a result of the suggested patent analysis, four technologies out of twenty two in details classification were selected, which showed outstanding technology competitiveness, high patent share and increasing rates as well as high recent-patent-ratios and triad-patent-family-ratios. Each of the selected technologies scored more than 10 points in total, and the following four technologies were suggested as promising ones in the field of next generation mobile communication: 1) 3GPP based mobile communication, 2) beyond 4G mobile communication, 3) IEEE 802.16 based mobile communication, which are in medium classification of broadband mobile communication system, and 4) testing/certification system of mobile communication, which is in medium classification of mobile communication testing/certification system.

关键词： next generation mobile communication promising technology forecasting patent information patent analysis patent indicators

玉米青霉穗腐病接种方法及玉米新品种抗性分析

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玉米科学 2024年第4期32卷 102-109页

作者：那明慧赵睿杰陈晓旭王作英高增贵丹东农业科学院辽宁凤城118100 沈阳农业大学植物保护学院沈阳110161

近年由青霉Penicillium spp.引起的穗腐病逐年加重。抗病玉米品种的选育和利用是控制穗腐病的经济、安全和有效措施。本研究开展玉米抗青霉穗腐病的接种鉴定,连续3年(2020-2022)在不同环境下比较分析花丝喷雾法、花丝通道注射法、子粒... 详细信息

近年由青霉Penicillium spp.引起的穗腐病逐年加重。抗病玉米品种的选育和利用是控制穗腐病的经济、安全和有效措施。本研究开展玉米抗青霉穗腐病的接种鉴定,连续3年(2020-2022)在不同环境下比较分析花丝喷雾法、花丝通道注射法、子粒注射法、牙签法等接种方法对玉米青霉穗腐病抗性鉴定的效果。结果表明,子粒注射法、牙签法发病强度适中,能够使得不同品种在人工接种后表现出不同水平的抗性反应,较适合用于玉米抗青霉穗腐病鉴定接种,接种后发病稳定、抗感差异明显、鉴定结果准确。2021-2022年接种鉴定的玉米新品种中,对青霉穗腐病表现高抗、抗性、中抗、感病和高感的品种分别占7.8%、24.1%、28.6%、24.5%、15.0%。

关键词：玉米青霉穗腐病接种方法抗性鉴定

胡兰贵运用芪脉地黄汤治疗慢性肾小球肾炎经验撷菁

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山东中医杂志 2024年第6期43卷 635-638页

作者：马倩张维亮申娜陈小慧邓树文刘洋胡娜胡兰贵(指导) 广州中医药大学广东广州510120 山西省中医药研究院山西太原030012 山西中医药大学山西太原030024 不详

胡兰贵教授认为慢性肾小球肾炎发病以外邪侵袭为引,病机以脾肾两虚为本,湿热、瘀血为标,三焦气滞为要。临床治疗本病,胡教授遵循“以脉为根”“以平为期”“守变相参”的辨证思维,应用健脾益肾、清热除湿、活血化瘀之芪脉地黄汤治之,并... 详细信息

胡兰贵教授认为慢性肾小球肾炎发病以外邪侵袭为引,病机以脾肾两虚为本,湿热、瘀血为标,三焦气滞为要。临床治疗本病,胡教授遵循“以脉为根”“以平为期”“守变相参”的辨证思维,应用健脾益肾、清热除湿、活血化瘀之芪脉地黄汤治之,并强调在疾病后期以膏方巩固收功。附验案1则。

关键词：慢性肾小球肾炎芪脉地黄汤脾肾两虚湿热瘀血三焦气滞胡兰贵

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聚合铝钛基絮凝剂的合成与应用

化工学报 2024年第3期75卷 1009-1018页

作者：潘娜田昌怀兰坤刘玉玉张芬芬高晓梅刘伟闫良国赵艳侠济南大学水利与环境学院山东济南250024 齐鲁工业大学(山东省科学院)环境科学与工程学院山东济南250353

聚合铝钛基絮凝剂是一种新型的高分子聚合絮凝剂,研究证明其具有铝盐絮凝剂有机物去除效率高、成本低的优点,也具有钛盐混凝剂低毒性、生物相容性和沉降性能好的优点。研究通过慢速滴碱法与电渗析法制备了两种聚合氯化铝钛絮凝剂(PATC)... 详细信息

聚合铝钛基絮凝剂是一种新型的高分子聚合絮凝剂,研究证明其具有铝盐絮凝剂有机物去除效率高、成本低的优点,也具有钛盐混凝剂低毒性、生物相容性和沉降性能好的优点。研究通过慢速滴碱法与电渗析法制备了两种聚合氯化铝钛絮凝剂(PATC),对两种不同制备方法的PATC进行了絮凝剂结构、絮凝效果以及絮凝动力学三个方面的研究。实验结果表明E-PATC的中、高聚体比S-PATC多3.89%,Zeta电位高8.9 mV。对两者的絮凝动力学进行了对比,发现E-PATC的生长速度快,絮体粒径大,易沉降。综上,E-PATC与S-PATC的絮凝效果相差不大,但E-PATC絮凝产生的絮体粒径大,不易破碎,具有更好的沉降性。

关键词：聚合铝钛基絮凝剂混凝聚合电化学水处理

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