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Survivability of freeze-dried probiotic Pediococcus pentosaceus strains GS4,GS17 and Lactobacillus gasseri(ATCC 19992)during storage with commonly used pharmaceutical excipients within a period of 120 days

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Asian Pacific Journal of Tropical Biomedicine 2017年第10期7卷 921-929页

作者： Mayur Bagad ram pande Vinay Dubey Asit Ranjan Ghosh Centre for Infectious Disease and Control Microbial Molecular Biology LaboratorySchool of Bio Science and Technology VIT UniversityVellore 632014India

Objective: To examine the survivability and stability of probiotic strains in presence and absence of pharmaceutical excipients for a long period of time at(4 ± 1)℃.Methods: The survival rates of probiotic strains, Pediococcus pentosaceus GS4(MTCC12683)(NCBI HM044322), GS17(NCBI KJ608061) and Lactobacillus gasseri(ATCC 19992), were evaluated. Probiotic strains were lyophilized individually and in combination of excipients(sorbitol, ascorbic acid, fructose and skim milk). The preparation was monitored for 120 d storing at(4 ± 1)℃. During storage, all the preparations were evaluated for viability and stability of probiotic properties like lactic acid production, antimicrobial effect, water activity, and adherence to epithelial cells.Results: Sorbitol, ascorbic acid and skim milk favoured the viability of freeze-dried cells and sustained probiotic properties during storage. Without excipients(control group),strains showed percentage of survivability not more than 70% while strains with excipients survived for 73%–93% for a long period of time.Conclusions: Commonly used excipients can be considered as a vehicle for delivering active principle in probiotic formulation and for sustaining the viability and stability of probiotic strains for a period of 120 d.

关键词： Viability Probiotics Pediococcus pentosaceus GS4 P.pentosaceus GS17 Lactobacillus gasseri Formulation

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Diet and habitat affinities in six raptor species in India

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Avian Research 2018年第4期9卷 354-362页

作者： Satish pande Reuven Yosef Federico Morelli Rajkumar Pawar ram Mone ELA Foundation C-9 Bhosale ParkSahakarnagrPune 411009India Department of Environmental Sciences Savitribai Phule Pune UniversityGaneshkhindPune 411007India Ben Gurion University of the Negev-Eilat Campus P.O.Box 27288000 EilatIsrael Department of Applied Geoinformatics and Spatial Planning Faculty of Environmental SciencesCzech University of Life Sciences PragueKamycká129165 00 Prague 6Czech Republic

Background: Sympatric species adapt to, and temporally or geographically segregate access to similar limiting factors.Methods: We compared nesting habitat and diet affinities of six raptor species in central India between the years 20062015.Results: A large composition of reptiles in the diet was characteristic for Circaetus gallicus, Spilornis cheela and Elanus caeruleus, while Aquila fasciata, Falco chicquera and Nisaetus cirrhatus show a higher proportion of birds. Species with greatest similarity of diet were C. gallicus and S. cheela. Considering the environmental characterization of areas where raptors built the nest, some species were ecologically closer than others. N. cirrhatus and S. cheela were related to the presence of water bodies, dry deciduous forest and evergreen forests, while E. caeruleus and C. gallicus preferred more open habitats. A. fasciata bred either on cliffs or trees, F. chicquera on trees or mobile towers, and the other four species built their nests exclusively in trees.Conclusions: We conclude that although there was overlap in diet and nesting ecology for a number of species, the geographical separation likely limits competition for resources.

关键词： Raptor species Diet Breeding area Niche overlap Resource partitioning

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Quantitative Structure-Permeation Relationships for Hydroxamic Acids Transport across Biological Membrane

Quantitative Structure-Permeation Relationships for Hydroxam...

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13th Asian Chemical Congress

作者： pande,rama RAJWADE,P.ram School of Studies in chemistry Pt.Ravishankar Shukla UniversityRaipurChhattisgarhIndia-492010

In this study,biological membrane such as inner layer of the egg of Gall domesticus(hen) was used as controlling barriers for permeation of some drug like molecule hydroxamic acids with different molecular weight and ... 详细信息

关键词： Apparent permeability logD QSPR Hydroxamic Acid PCA and PLS

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LOCAL SCOUR AROUND BRIDGE ABUTMENTS

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International Journal of Sediment Research 2002年第1期17卷 48-74页

作者： Er. Shri ram CHAURASIA1 and pande B.B. LAL2 1 Assistant Professor, Civil Engineering Department, Madan Mohan Malaviya Engineering College, Gorakhpur-273 010 (U.P.) India, 2 Dr., Principal, Mada Assistant Professor Civil Engineering Department Madan Mohan Malaviya Engineering CollegeGorakhpur-273010 (U.P.) India Dr. Principal Madan Mohan Malaviya Engineering College Gorakhpur-273010(U.P.) India

This paper summarizes the design equations for local scour depth around bridge abutments as proposed by different investigators, and also the different factors, affecting the scour depth. Methods for the estimation of local scour depth based on dimensional analysis are also presented. The design methods based on empirical relations such as flow depth, flow intensity, sediment characteristics, abutments shape and size, alignment, and approach channel geometry are also discussed. Since clear-water scour is the maximum scour depth, the equation for clear-water scour is recommended for the computation of local scour depth around bridge abutments for uniformly and non- uniformly graded sediments.

关键词： Local scour Bridge abutments Flood plain Scour depth

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