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Identific tion of quantitative trait loci and associated candidate genes for pregnancy success in Angus–Brahman crossbred heifers

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Journal of Animal Science and Biotechnology 2024年第1期15卷 162-170页

作者： Quinn A.Hoorn gabriel A.Zayas Eduardo E.Rodriguez Laura M.Jensen raluca g.mate.scu Peter J.Hansen Department of Animal Sciences Donald Henry Barron Reproductive and Perinatal Biology Research Programand the Genetics InstituteUniversity of FloridaGainesvilleFLUSA School of Applied Systems Biology La Trobe UniversityBundooraVIC 3083Australia

Background In beef cattle, more than 50% of the energy input to produce a unit of beef is consumed by the female that produced the calf. Development of genomic tools to identify females with high genetic merit for reproductive function could increase the profitability and sustainability of beef *** genome-wide association studies(gWAS) were performed using a single-step genomic best linear unbiased prediction approach on pregnancy outcome traits from a population of Angus–Brahman crossbred heifers. Furthermore, a validation gWAS was performed using data from another farm. Heifers were genotyped with the Bovine ggP F250 array that contains 221,077 SNPs. In the discovery population, heifers were bred in winter breeding seasons involving a single round of timed artificial insemination(AI) followed by natural mating for 3 months. Two phenotypes were analyzed: pregnancy outcome to first-service AI(PAI;n = 1,481) and pregnancy status at the end of the breeding season(PEBS;n = 1,725). The heritability was estimated as 0.149 and 0.122 for PAI and PEBS, respectively. In the PAI model, one quantitative trait locus(QTL), located between 52.3 and 52.5 Mb on BTA7, explained about 3% of the genetic variation, in a region containing a cluster of γ-protocadherin genes and SLC25A2. Other QTLs explaining between 0.5% and 1% of the genetic variation were found on BTA12 and 25. In the PEBS model, a large QTL on BTA7 was synonymous with the QTL for PAI, with minor QTLs located on BTA5, 9, 10, 11, 19, and 20. The validation population for pregnancy status at the end of the breeding season were Angus–Brahman crossbred heifers bred by natural mating. In concordance with the discovery population, the large QTL on BTA7 and QTLs on BTA10 and 12 were *** In summary, QTLs and candidate SNPs identified were associated with pregnancy outcomes in beef heifers, including a large QTL associated with a group of protocadherin genes. Confirmation of these associations

关键词： Beef cattle Fertility gWAS QTL

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Exploring the genetic control of sweat gland characteristics in beef cattle for enhanced heat tolerance

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Journal of Animal Science and Biotechnology 2024年第5期15卷 1820-1831页

作者： Aakilah S.Hernandez gabriel A.Zayas Eduardo E.Rodriguez Kaitlyn M.Sarlo Davila Fahad Rafiq Andrea N.Nunez Cristiane goncalves Titto raluca g.mate.scu Department of Animal Science North Carolina State UniversityRaleighNCUSA Department of Animal Sciences University of FloridaGainesvilleFLUSA. Ruminant Diseases and Immunology Research Unit National Animal Disease CentersUnited States Department of Agriculture1920 Dayton AvenueAmesIA 50010USA. Department of Animal Science Iowa StateAmesIAUSA School of Animal Sciences and Food Engineering University of Sao PauloAv Duque de Caxias Norte 225PirassunungaSao Paulo 13635‑900Brazil

Background Thermal stress in subtropical regions is a major limiting factor in beef cattle production systems with around$369 million being lost annually due to reduced *** stress causes numerous physiological and behavioral disturbances including reduced feed intake and decreased production *** utilize various physiological mechanisms such as sweating to regulate internal *** in these traits can help identify genetic variants that control sweat gland properties and subsequently allow for genetic selection of cattle with greater *** This study used 2,401 Brangus cattle from two commercial ranches in *** phenotypes that contribute to an animal's ability to manage heat stress were calculated from skin biopsies and included sweat gland area,sweat gland depth,and sweat gland *** animals were genotyped with the Bovine ggP F250K,and BLUPF90 software was used to estimate genetic parameters and for genome Wide Association *** Sweat gland phenotypes heritability ranged from 0.17 to 0.42 indicating a moderate amount of the phenotypic variation is due to genetics,allowing producers the ability to select for favorable sweat gland properties.A weighted single-step gWAS using sliding 10 kb windows identified multiple quantitative trait loci(QTLs)explaining a significant amount of genetic *** located on BTA7 and BTA12 explained over 1.0%of genetic variance and overlap the ADgRV1 and CCDC168 genes,*** variants identified in this study are implicated in processes related to immune function and cellular proliferation which could be relevant to heat *** of Origin Alleles(BOA)were predicted using local ancestry in admixed populations(LAMP-LD),allowing for identification of markers'origin from either Brahman or Angus ancestry.A BOA gWAS was performed to identify regions inherited from particular ancestral breeds that might have a significant impact on sweat gland pheno

关键词： gWAS Heritability Skin Thermotolerance

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B2 adrenergic receptors and morphological changes of the enteric nervous system in colorectal adenocarcinoma

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World Journal of gastroenterology 2017年第7期23卷 1250-1261页

作者： raluca Niculina Ciurea Ion Rogoveanu Daniel Pirici georgica-Costinel Tartea Costin Teodor Streba Cristina Florescu Bogdan Catalin Ileana Puiu Elena-Anca Tartea Cristin Constantin Vere Department of Pathology University of Medicine and Pharmacy of Craiova Department of gastroenterology University of Medicine and Pharmacy of Craiova Department of Research Methodology University of Medicine and Pharmacy of Craiova Department of Internal Medicine University of Medicine and Pharmacy of Craiova Department of Cardiology University of Medicine and Pharmacy of Craiova Department of Physiology University of Medicine and Pharmacy of Craiova Department of Pediatrics University of Medicine and Pharmacy of Craiova

To study the morphology of the enteric nervous system and the expression of beta-2 adrenergic (B2A) receptors in primary colorectal *** this study, we included forty-eight patients with primary colorectal cancer and nine patients for control tissue from the excision of a colonic segment for benign conditions. We determined the clinicopathological features and evaluated the immunohistochemical expression pattern of B2A receptors as well as the morphological changes of the enteric nervous system (ENS). In order to assess statistical differences, we used the student t-test for comparing the means of two groups and one-way analysis of variance with Bonferroni’s post hoc analysis for comparing the means of more than two groups. Correlations were assessed using the Pearson’s correlation ***2A receptors were significantly associated with tumor grading, tumor size, tumor invasion, lymph node metastasis (P gnificant associations with gender, CRC location and gross appearance (P > 0.05). We observed, on one hand, a decrease of the relative area for both Auerbach and Meissner plexuses with the increase of the tumor grading, and on the other hand, an increase of the relative area of other nervous elements not in the Meissner plexus or in the Auerbach plexus with the tumor grading. For g1 tumors we found that epithelial B2A area showed an inverse correlation with the Auerbach plexus areas [r(14) = -0.531, P g2 tumors, epithelial B2A areas showed an indirect variation with both the Auerbach plexus areas [r(14) = -0.453, P g3 tumors, the inverse dependence increased for both Auerbach [r(14) = -0.587, P genesis and can be utilized as prognostic factors. Furthermore, study of the ENS in colorectal cancer m

关键词： Beta-2 adrenergic receptors Enteric nervous system Colorectal adenocarcinoma Spectral unmixing immunohistochemistry Tumor grading

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Endoscopic ultrasound-guided fine-needle aspiration pancreatic adenocarcinoma samples yield adequate DNA for next-generation sequencing:A cohort analysis

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World Journal of gastroenterology 2023年第18期29卷 2864-2874页

作者： Stefania Bunduc Bianca Varzaru Razvan Andrei Iacob Andrei Sorop Ioana Manea Andreea Spiridon raluca Chelaru Adina Emilia Croitoru gabriel Becheanu Mona Dumbrava Simona Dima Irinel Popescu Cristian gheorghe Faculty of Medicine Carol Davila University of Medicine and PharmacyBucharest 020021Romania Digestive Diseases and Liver Transplantation Center Fundeni Clinical InstituteBucharest 022328Romania Center of Excellence in Translational Medicine Fundeni Clinical InstituteBucharest 022328Romania

BACKgROUND genetic tests are increasingly performed for the management of unresectable pancreatic *** genotyping aimed samples current guidelines recommend using core specimens,although based on moderate quality ***,in clinical practice among the endoscopic ultrasound(EUS) guided tissue acquisition methods,fine needle aspiration(FNA) is the most widely *** To assess the adequacy for next generation sequencing(NgS) of the DNA yielded from EUS-FNA pancreatic adenocarcinoma(PDAC) *** Between November 2018 and December 2021,105 patients with PDAC confirmed by EUS-FNA were included in the study at our tertiary gastroenterology *** 22 gauge(g) or 19g FNA needles were *** pass was dedicated to DNA *** concentration and purity(A260/280,A260/230) were assessed by *** assessed the differences in DNA parameters according to needle size and tumor characteristics(size,location) and the adequacy of the extracted DNA for NgS(defined as A260/280 ≥ 1.7,and DNA yield:≥ 10 ng for amplicon based NgS,≥ 50 ng for whole exome sequencing [WES],≥ 100 ng for whole genome sequencing [WgS]) by analysis of variance and ttest ***,we compared DNA purity parameters across the different DNA yield *** Our cohort included 49% male patients,aged 67.02 ± 8.38 *** 22g needle was used in 71%of the *** DNA parameters across our samples varied as follows:DNA yield:1289 ng(inter quartile range:534.75-3101),A260/280 = 1.85(1.79-1.86),A260/230 = 2.2(1.72-2.36).DNA yield was > 10 ng in all samples and > 100 ng in 93% of them(one sample g).There were no significant differences in the concentration and A260/280 between samples by needle *** size was the only independent predictor of A260/230 which was higher in the 22g samples(P =0.038).NgS adequacy rate was 90% for 19g samples regardless of NgS type,and for 22g samples it reached 89% for WgS adequacy and 91% for WES and

关键词： Pancreatic adenocarcinoma Endoscopic ultrasound guided fine needle aspiration Next generation sequencing DNA yield Needle size genetic testing

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giant androgen-producing adrenocortical carcinoma with atrial flutter: A case report and review of the literature

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World Journal of Clinical Cases 2021年第20期9卷 5575-5587页

作者： Mircea-Florin Costache raluca-Elena Arhirii Simona-Juliette Mogos Corina Lupascu-Ursulescu Cezara-Ioana Litcanu Adi-Ionut Ciumanghel Catalina Cucu Cristina-Mihaela ghiciuc Antoniu-Octavian Petris Nicolae Danila Surgery Clinic Saint Spiridon University Clinical Emergency HospitalIasi 700111Romania Cardiology Clinic Saint Spiridon University Clinical Emergency HospitalIasi 700111Romania Department of Endocrinology Faculty of MedicineGrigore T.Popa University of Medicine and PharmacyIasi 700115Romania Endocrinology Clinic Saint Spiridon University Clinical Emergency HospitalIasi 700111Romania Department of Radiology and Imaging Sciences Grigore T.Popa University of Medicine and PharmacyIasi 700115Romania Radiology Clinic Saint Spiridon University Clinical Emergency HospitalIasi 700111Romania Radiotherapy Clinic Regional Institute of OncologyIasi 700483Romania Anesthesia and Intensive Care Department Grigore T.Popa University of Medicine and PharmacyIasi 700115Romania Anesthesia and Intensive Care Department Saint Spiridon University Clinical Emergency HospitalIasi 700111Romania Histopatology Department Saint Spiridon University Clinical Emergency HospitalIasi 700111Romania Department of Pharmacology Clinical Pharmacology and AlgesiologyFaculty of MedicineGrigore T.Popa University of Medicine and PharmacyIasi 700115Romania Department of Cardiology Faculty of MedicineGrigore T.Popa University of Medicine and PharmacyIasi 700115Romania Surgery Clinic Faculty of MedicineGrigore T.Popa University of Medicine and PharmacyIasi 700115Romania

BACKgROUND Adrenocortical carcinoma(ACC),the second most aggressive malignant tumor,lacks epidemiological data worldwide;therefore,every new case can improve the understanding of the pathology and treatment of this *** SUMMARY We present the case of a 66-year-old Caucasian woman with a giant androgenproducing ACC(21 cm×17 cm×12 cm;2100 g),without metastases,which unusually presented with an acute onset of atrial flutter and congestive heart *** cardiac complications observed in our case support the hypothesis that androgen excess in women is a cardiovascular risk *** excess in women can be a rare cause of reversible dilated cardiomyopathy,therefore a comprehensive approach to the patient is essential to improve the recognition of androgen-secreting *** atrial flutter was remitted after initiation of drug treatment during *** severe heart failure was totally remitted at 6 mo after radical open surgery to remove the giant *** Radical open surgery to remove a giant androgen-producing ACC was the firstline treatment to cure the excess of androgen,which determined the total remission of cardiac complications at 6 mo after surgery in the women of this case report.

关键词： Adrenocortical carcinoma Adrenalectomy Androgen secreting tumor Heart failure Atrial flutter Case report

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