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In Vitro Evaluation of Gamma Irradiation on a Gel Formulation of Cratylia Mollis: Rheological Proporties and Microbiological Control

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Journal of Cosmetics, Dermatological Sciences and Applications 2012年第2期2卷 45-50页

作者： Maria Helena Madruga Lima-Ribeiro Ralph Santos-Oliveira Mauricelia Firmino De Santana Terezinha De Jesus Andreoli Pinto Irene Satiko Kikuchi Cheila Goncalves Mothe Luana Cassandra Breitenbach Barroso Coelho Maria Tereza Dos santos Correia Ana Maria Dos Anjos Carneiro-Leao Laboratory of Immunopathology Keizo Asami(LIKA) Federal University of PernambucoRecifeBrazil Nuclear Engineering Institute Laboratory of NanoradiopharmaceuticalsRio de JaneiroBrazil Biochemistry Department Federal University of PernambucoRecifeBrazil Pharmacy Department University of Sao PauloSao PauloBrazil School of Chemistry Federal University of Rio de JaneiroRio de JaneiroBrazil Departament of Morphology and Animal Physiology Federal Rural University of PernambucoRecifeBrazil

Lectin Cramoll-1,4, obtained from Cratylia mollis seeds (beans camaratu) was structurally characterized, biologically and pharmacologically, but its use as a biopharmaceutical is not well documented. The objective of this study is to propose a biopharmaceutical formulation lectin Cramoll-1,4, test their hemagglutinating properties in vitro as well as the use of gamma radiation as a continuous process of decontamination formulation. It was made of the extraction and purification Cramoll-1,4, was developed a gel formulation using Carbopol? as a vehicle, at concentrations of 50, 100, 200, 300 and 600 μg was irradiated with 60Co gamma rays in a dose of 7.549 kGy·h–1. The proposed formulation at a concentration of 300 μg produced an increase in the hemagglutinating units Cramoll-1,4 due to the synergistic effect caused by gamma radiation. Considering the diverse use of lectins, specific molecular and structural factors, as well as changes resulting from its formulation, concentration, irradiation and route of administration is of utmost importance to continue the studies in vitro, for subsequent application in vivo to characterize the physiological and molecular processes involved in the response and cellular effects.

关键词： Gamma Radiation Natural Products Hemagglutinating Activity Cramoll-1 4

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ID7 Isolated from Bauhinia variegata Stem Inhibits Tumor Progression and Metastatic Mechanisms of Triple Negative Breast Cancer in Vivo

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Journal of Pharmacy and Pharmacology 2019年第7期7卷 368-384页

作者： Kamilla Monteiro dos santos Izabela Natalia Faria Gomes Renato JoséSilva oliveira Fernanda E.Pinto Bruno oliveira Fabrício P.Batista Rafael Cesar Russo Chagas Hélio B.santos ralph G.Thomé Wanderson Romao Rui Manuel Reis Maria Luiza Vilela Oliva Rosy Iara Maciel de Azambuja Ribeiro Laboratory of Experimental Pathology Federal University of Sao Joao del-Rei(UFSJ)DivinópolisMGBrazil Josédo Rosário Vellano University UnifenasDivinópolisMGBrazil Molecular Oncology Research Center(CPOM) Barretos Cancer HospitalBarretosSPBrazil Petroleomic and Forensic Chemistry Laboratory Department of ChemistryFederal Institute of Espírito Santo(IFES)VitóriaESBrazil Chemistry Department Federal University of Sao PauloBrazil Tissue Processing Laboratory Federal University of Sao Joao del-Rei(UFSJ)DivinópolisMGBrazil Life and Health Sciences Research Institute(ICVS) Medical SchoolUniversity of MinhoBragaPortugal 3ICVS/3B’s-PT Government Associate Laboratory GuimaraesBragaPortugal

Background:The breast cancer has been the most common form of cancer among women.The triple negative subtype represents 20%of all breast cancer cases in the world and is standing out by affecting young women and being aggressive.The main cause of death of patients with cancer is due to metastasis,which can reach the liver and lungs.Objective:The activities of ID7 fraction of the stems of Bauhinia variegata L.on breast cancer,lung metastasis and liver inflammatory process were evaluated.Method:ID7 was characterized by mass-spectrometry.The viability of murine mammary cells(4T1)treated with ID7 was assessed by MTT,trypan blue and fluorescence assay and viability of BT-20,MDA-MB-231 and MCF-7 human breast cancer tumor lines by MTS.The cell migration,invasion using matrigel and adhesion were performed.The expression of cell death proteins was quanitified by western blot and the gelatinases by zimogram.The ID7 activity of the tumor(4T1)and metastatic progession in vivo was evaluated.Results:ID7 reduced the 4T1 and MDA-MB-231viability and increased the late apoptosis,inhibited the 4T1 migration and invasion,increased the 4T1 adhesion and decreased the secreted active gelatinases.ID7 also increased the expression of PARP,caspase-7 and caspase-8,RIP and TNF-R1.In vivo,the ID7 decreased the volume and weight of the tumors and decreased lung metastasis and inflammation in the liver.The characterization showed mainly the presence of oleic acid,myricetin,quercetin and kaempferol in ID7.Conclusion:Thus,it was found that ID7 fraction exhibits selective antitumor and on the mechanisms of breast cancer metastasis activity,preventing lung metastasis and inflammation in the liver.It is suggested that fatty acids and flavonoids are correlated with such activities.

关键词： 4T1 cells MDA-MB-231 medicinal plants kaempferol gelatinases apoptosis cell viabilty in vivo

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Adjustment of Mathematical Equations to Determine the Area Leaf of <i>Cordia myxa</i>L.

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Agricultural Sciences 2020年第7期11卷 609-616页

作者： Michelle Teixeira Araujo Ellen Maurício Faria Gabriel Linhares Cunha Paloma de Moraes Nunes Vinicius de Souza oliveira Karina Tiemi Hassuda dos santos Omar Schmildt Antelmo ralph Falqueto Mônica Maria Pereira Tognella Edilson Romais Schmildt Center North of Espírito Santo Federal University of Espírito Santo S&#227 o Mateus Espírito Santo Brazil Center for Agricultural Sciences and Engineering Federal University of Espírito Santo Alegre Espírito Santo Brazil

The objective of this study was to obtain mathematical equations through the leaf dimensions to estimate the area leaf of Cordia myxa L. 392 leaves of plants were collected, located in the municipality of São Mateus, North of the State of Espírito Santo, Brazil, 300 of which were used to adjust the equations and 92 for validation. Of all the leaves, the largest length (L) and maximum width (W) were measured, product of multiplying the length with the width (LW) and the real leaf area (RLA). Models equation first degree linear, quadratic and power were fitted, where RLA was the dependent variable as a function of L, W and LW as an independent variable. All equations were validated according to appropriate criterion. Thus, the power model ELA = 0.84(LW)0.9749, based on the product of multiplication of length with width (LW) is the most suitable for estimating the leaf area of Cordia myxa L.

关键词： Non-Destructive Method Validated Modeling

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