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The Transcriptional Activity of LAMP3 Gene Involved in Autophagocytosis in Colorectal Cancer LAMP3 Expression in Colorectal Cancer

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Journal of Biosciences and Medicines 2017年第12期5卷 24-36页

作者： Martyna Bednarczyk Malgorzata Muc-Wierzgon Nikola Zmarzly Celina Kruszniewska-rajs Beniamin Grabarek Agata Kazmierczak Bartlomiej skowronek Klaudia simka Dariusz Waniczek Katarzyna Walkiewicz Urszula Mazurek Department of Internal Medicine School of Public Health in BytomMedical University of SilesiaKatowicePoland Department of Molecular Biology School of Pharmacy with the Division of Laboratory Medicine in SosnowiecMedical University of SilesiaKatowicePoland Chair of General Colorectal and Polytrauma SurgeryDepartment of Propaedeutics SurgeryMedical University of SilesiaKatowicePoland

Background: Autophagocytosis is a biological process involving the controlled distribution of cell fragments and organelles in order to obtain an additional source of energy. LAMP3 (lysosome-associated membrane protein 3) is a heavily glycosylated integral membrane protein located mainly in the lysosomal membrane. Recent studies have shown that it participates in tumor metastasis and drug resistance. Its main role is contribution to tumor cells proliferation, migration and invasion. The aim of this study was to determine changes in transcriptional activity of LAMP3 at various stages of colorectal cancer. In addition, an attempt was made to select miRNAs potentially regulating LAMP3 expression using bioinformatic databases. Methods: The study was conducted on healthy colon samples and colon cancer samples in four stages. Molecular analysis included the extraction of total RNA, purification of the obtained extracts, expression profile analysis using oligonucleotide microarray technique and in silico determination of miRNAs potentially regulating the LAMP3 expression. Results: The level of LAMP3 expression is higher in colorectal adenocarcinoma cells than in non-cancerous cells and depends on the stage of the disease. Conclusions: LAMP3 may promote cancer progression, metastasis and cause the resistance to treatment.

关键词： Autophagy Colorectal Cancer LAMP3 Microarrays miRNA

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