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Cardiogenic differentiation of mesenchymal stem cells on elastomeric poly (glycerol sebacate)/collagen core/shell fibers

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World Journal of Cardiology 2013年第3期5卷 28-41页

作者： rajeswari ravichandran Jayarama Reddy Venugopal Subramanian Sundarrajan Shayanti Mukherjee Seeram Ramakrishna Faculty of Engineering Nanoscience and Nanotechnology Initiative National University of Singapore Department of Mechanical Engineering National University of Singapore

AIM: To facilitate engineering of suitable biomaterials to meet the challenges associated with myocardial infarction. METHODS: Poly (glycerol sebacate)/collagen (PGS/ collagen) core/shell fibers were fabricated by core/ shell electrospinning technique, with core as PGS and shell as collagen polymer; and the scaffolds were characterized by scanning electron microscope (SEM), fourier transform infrared spectroscopy (FTIR), contact angle and tensile testing for cardiac tissue engineering. Collagen nanofibers were also fabricated by electrospinning for comparison with core/shell fibers. Studies on cell-scaffold interaction were carriedout using cardiac cells and mesenchymal stem cells (MSCs) co-culture system with cardiac cells and MSCs separately serving as positive and negative controls respectively. The co-culture system was characterized for cell proliferation and differentiation of MSCs into cardiomyogenic lineage in the co-culture environment using dual immunocytochemistry. The co-culture cells were stained with cardiac specific marker proteins like actinin and troponin and MSC specific marker protein CD 105 for proving the cardiogenic differentiation of MSCs. Further the morphology of cells was analyzed using ***: PGS/collagen core/shell fibers, core is PGS polymer having an elastic modulus related to that of cardiac fibers and shell as collagen, providing natural environment for cellular activities like cell adhesion, proliferation and differentiation. SEM micrographs of electrospun fibrous scaffolds revealed porous, beadless, uniform fibers with a fiber diameter in the range of 380 ± 77 nm and 1192 ± 277 nm for collagen fibers and PGS/collagen core/shell fibers respectively. The obtained PGS/collagen core/shell fibrous scaffolds were hydrophilic having a water contact angle of 17.9 ± 4.6° compared to collagen nanofibers which had a contact angle value of 30 ± 3.2°. The PGS/collagen core/shell fibers had mechanical properties comparable to that of native

关键词： Mesenchymal stem cells Cardiac cells Coculture Cardiac patch Poly (glycerol sebacate) Core/ shell fibers.

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Effects of nanotopography on stem cell phenotypes

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World Journal of Stem Cells 2009年第1期1卷 55-66页

作者： rajeswari ravichandran Clarisse CH Ng Casey K Chan Michael Raghunath Seeram Ramakrishna Division of Bioengineering Faculty of EngineeringNational University of SingaporeSingapore 117574Singapore Department of Mechanical Engineering Faculty of Engineering National University of SingaporeSingapore 117576Singapore Nanoscience and Nanotechnology Initiative National University of Singapore2 Engineering Drive 3Singapore 117576Singapore Department of Restorative Dentistry Faculty of DentistryNational University of SingaporeSingapore 119074 Singapore Department of Orthopaedic Surgery Yong Loo Lin School of MedicineNational University of Singapore Singapore 119074Singapore Department of Biochemistry Yong Loo Lin School of Medicine8 Medical DriveNational University of SingaporeSingapore 117597Singapore

Stem cells are unspecialized cells that can self renew indefinitely and differentiate into several somatic cells given the correct environmental *** the stem cell niche,stem cell-extracellular matrix(ECM)interactions are crucial for different cellular functions,such as adhesion,proliferation,and ***, in addition to chemical surface modifications,the importance of nanometric scale surface topography and roughness of biomaterials has increasingly becoming recognized as a crucial factor for cell survival and host tissue acceptance in synthetic *** review describes the influence of nanotopography on stem cell phenotypes.

关键词： Stem cells Nanofibers Nanotopography Biomaterials Extracellular matrix Differentiation

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