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Critical review of EPS production, synthesis and composition for sludge flocculation

Journal of Environmental Sciences 2018年第4期30卷 225-245页

作者： Klai Nouha Ram Saurabh Kumar Sellamuthu Balasubramanian rajeshwar dayal tyagi Universite du Quebec Institut national de la Recherche Scientifique Centre Eau Terre & Environnement 490 de la CouronneQuebec G1K 9A9 Canada Institut Armand-Frappier 531 boul. des Prairies Laval Quebec H7V 1B7 Canada

Extracellular polymeric substances（EPS） produced by microorganisms represent biological macromolecules with unfathomable potentials and they are required to be explored further for their potential application as a bioflocculant in various wastewater sludge treatment. Although several studies already exist on biosynthetic pathways of different classical biopolymers like alginate and xanthan, no dedicated studies are available for EPS in sludge. This review highlights the EPS composition, functionality, and biodegradability for its potential use as a carbon source for production of other metabolites. Furthermore, the effect of various extraction methods（physical and chemical） on compositional, structural, physical and functional properties of microbial EPS has been addressed. The vital knowledge of the effect of extraction method on various important attributes of EPS can help to choose the suitable extraction method depending upon the intended use of EPS. The possible use of different molecular biological techniques for enhanced production of desired EPS was summarized.

关键词： Extracellular polymeric substances Metabolic pathways Extraction methods Flocculation properties

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ORIGINAL ARTICLE Dynamics of bacterial community at varying sludge retention time within membrane bioreactor treating synthetic hospital wastewater

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Systems Microbiology and Biomanufacturing 2021年第4期1卷 471-482页

作者： Bhagyashree Tiwari Balasubramanian Sellamuthu Sarah Piche-Choquette Patrick Drogui rajeshwar dayal tyagi Marc Antoine Vaudreuil Sebastien Sauve Gerardo Buelna Rino Dube INRS-Eau Terre et EnvironnementQuebecQC G1K9A9Canada Departement de Radiologie Radio-Oncologie et Medecine NucleaireCentre Hospitalier de l’Universite de MontrealMontrealQC H2X 0A9Canada INRS-Institut Armand-Frappier LavalQC H7V 1B7Canada BOSK Bio ProductsQuebecCanada School of Technology Huzhou UniversityHuzhouChina Department of Chemistry Universite de MontrealMontrealQCCanada Centre de Recherche Indust

The study was conducted to investigate the effect of sludge retention on bacterial community composition of membrane bioreactor(MBR)treating synthetic hospital *** removal of four pharmaceuticals,namely carbamazepine,estradiol,venlafaxine,and ibuprofen in MBR,was studied at varying sludge retention time(SRT)duration of 100,45,and 15 days and hydraulic retention time(HRT)of 18 *** removal of ibuprofen and estradiol was constant at varying SRT;however,a negligible removal of carbamazepine and low removal of venlafaxine was observed(<20%).The study suggested that the SRT of 45 days in MBR could provide maximum treatment efficiency via decreasing membrane *** effect of sludge age and pharmaceutical presence on the bacterial community was investigated via high-throughput *** study reveals that the variation in SRT affects the dynamics of the bacterial community *** instance,the dominant bacterium Caldimonas of SRT 100 was disappeared at lower ***,the profile of the dominant genus of the SRTs varied greatly from each other.

关键词： Membrane bioreactor Pharmaceuticals Sludge retention time Bacterial community

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Optimization of Rubber Seed Oil Transesterification to Biodiesel Using Experimental Designs and Artificial Neural Networks

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Green and Sustainable Chemistry 2018年第1期8卷 39-61页

作者： Konan Edmond Kouassi Abollé Abolle Abolle Kouassi Benjamin Yao David Boa Kopoin Adouby Patrick Drogui rajeshwar dayal tyagi Laboratoire de Procédés Industriels de Synthèse de l’Environnement et des Energies Nouvelles Groupe des Procédés et Environnement Institut National Polytechnique Félix Houphou&#235 t-Boigny Yamoussoukro C&#244 te d’Ivoire Laboratoire de Thermodynamique et Physico-Chimie du Milieu UFR Sciences Fondamentales et Appliquées Université Nangui Abrogoua Abidjan C&#244 te d’Ivoire Institut National de la Recherche Scientifique (INRS-Centre Eau Terre et Environnement) Université du Québec Québec Canada

The development of biofuels is driven both by concern about the greenhouse effect and by interest in the opportunities for exploitation of biomass of agricultural origin. In order to improve the yield and quality of biodiesel through modeling and optimization, several studies are in progress. In this paper, biodiesel produced from rubber seed oil in the homogeneous transesterification is studied using a Plackett-Burman experimental design, a full factorial design, a central composite design and an Artificial Neural Network (ANN) coupled with a Genetic Algorithm (GA).Variables such as temperature, stirring speed, reaction time, type of alcohol, and type of catalyst are studied to obtain the best specific gravity and kinematic viscosity. Type of alcohol and type of catalyst have the greatest effect on the two responses, with ethanol (alcohol) and sulphuric acid (catalyst) producing the best results. The specific gravity and kinematic viscosity changes recorded during the transesterification process followed the first and second order polynomial models, respectively. The ANN coupled with GA was used to optimize the two responses simultaneously. Global optimal values of specific gravity (0.883) and kinematic viscosity (6.76 cSt) were recorded when a temperature of 90°C, a stirring speed of 305 rpm, and a treatment time of 141 min were imposed.

关键词： Rubber Seed Oil Biodiesel Transesterification Specific Gravity Kinematicviscosity

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Cassava Peel Starch as a Raw Material for Polyhydroxyalkanoates Synthesis by Cupriavidus necator

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Open Journal of Applied Sciences 2024年第11期14卷 3127-3144页

作者： Ida Diribissakou Magnoudéwa Bassaї Bodjona Song Yan Julien G. Mahy Gado Tchangbedji rajeshwar dayal tyagi Benjamin K. Yao Patrick Drogui Waste Management Treatment and Valorization Laboratory (GTVD) Faculty of Sciences University of Lom Lom Togo INRS Water Earth and Environment Qubec Canada Department of Chemical Engineering-Nanomaterials Catalysis & Electrochemistry University of Lige Lige Belgium Houphout Boigny National Polytechnic Institute (INPHB) Yamoussoukro Cte dIvoire

The environmental problems caused by plastics of fossil origin are well known. To reduce harmful impact on the environment, bacterial-based plastics, such as polyhydroxyalkanoates (PHAs), are a promising solution. Microbial PHAs can be produced using abundant and inexpensive agricultural by-products as raw material. In this study, the potential use of Cupriavidus necator 11599 for the bioconversion of cassava starch into biodegradable PHAs was explored. Although Cupriavidus necator 11599 is a well-known PHA producer, it cannot grow directly on starch. Thus, acid hydrolysis was carried out on the starch extracted from cassava peels to obtain fermentable sugars. Optimal concentration of reducing sugars (RSs) was obtained by hydrolysis of cassava peel starch with sulfuric acid concentrations of 0.4 N and 0.6 N, at 95˚C and 4 h. The hydrolyzed starch was used for PHA production in Erlenmeyer flasks using reducing sugars (RSs) concentrations ranging from 10 g/L to 25 g/L. The best RS concentration 20 g/L and 25 g/L gave 85.13% ± 1.17% and 89.01% ± 2.49% of biomass PHA content and biomass concentrations of 8.18 g/L and 8.32 g/L, respectively in 48 hours. This research demonstrates that cassava peel starch as an inexpensive feedstock could be used for PHA production, paving the way for the use of other starchy materials to make bioplastics.

关键词： Polyhydroxyalkanoate Cupriavidus necator Cassava Peels Starch Acid Hydrolysis

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