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Genetic polymorphism in Leishmania infantum isolates from human and animals determined by nagt PCR-RFLP

Infectious Diseases of Poverty 2018年第1期7卷 523-530页

作者： Adil El Hamouchi Sofia El Kacem rajaa ejghal Meryem Lemrani Laboratory of Parasitology and Vector-Borne-Diseases Institut Pasteur du MarocCasablancaMorocco

Background:Leishmania infantum is the causative agent of human visceral leishmaniasis(VL)and sporadic human cutaneous leishmaniasis(CL)in the Mediterranean *** genetic variation of the Leishmania parasites may result in different phenotypes that can be associated with the geographical distribution and diversity of the clinical *** main objective of this study was to explore the genetic polymorphism in *** isolates from human and animal hosts in different regions of ***:The intraspecific genetic variability of 40 Moroccan *** MON-1 strains isolated from patients with VL(n=31)and CL(n=2)and from dogs(n=7)was evaluated by PCR-RFLP of nagt,a single-copy gene encoding N-acetylglucosamine-1-phosphate *** a more complete analysis of *** polymorphism,we included the restriction patterns of nagt from 17 strains available in the literature and patterns determined by in-silico digestion of three sequences from the GenBank ***:Moroccan *** strains presented a certain level of genetic diversity and six distinct nagt-RFLP genotypes were *** of the six genotypes were exclusively identified in the Moroccan population of ***:variant M1(15%),variant M2(7.5%),and variant M3(2.5%).The most common genotype(65%),variant 2(2.5%),and variant 4(7.5%),were previously described in several countries with endemic *** analysis segregated our *** population into two distinct clusters,whereas variant M2 was clearly distinguished from both cluster I and cluster *** distribution highlights the degree of genetic variability among the Moroccan *** ***:The nagt PCR-RFLP method presented here showed an important genetic heterogeneity among Moroccan *** strains isolated from human and canine reservoirs with 6 genotypes *** of the six Moroccan nagt genotypes,have not been previously described and support the particular gen

关键词： Leishmania infantum Genetic variability N-acetylglucosamine-1-phosphate transferase PCR-RFLP Morocco

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