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Production and Characterization of Monoclonal Antibodies to Bluetongue Virus

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Virologica Sinica 2011年第1期26卷 8-18页

作者： Veerakyathappa Bhanuprakash Madhusudhan Hosamani Vinayagamurthy Balamurugan Pradeep narayan Gandhale Gnanavel Venkatesan raj Kumar Singh Division of Virology Indian Veterinary Research Institute Indian Veterinary Research Institute Project Directorate on Animal Disease Monitoring And Surveillance National Research Centre on Equines and Veterinary Type Culture Centre

In the present study,a total of 24 MAbs were produced against bluetongue virus (BTV) by polyethyleneglycol (PEG) mediated fusion method using sensitized lymphocytes and myeloma cells. All these clones were characterized for their reactivity to whole virus and recombinant BTV-VP7 protein,titres,isotypes and their reactivity with 24 BTV-serotype specific sera in cELISA. Out of 24 clones,a majority of them (n = 18) belong to various IgG subclasses and the remaining (n = 6) to the IgM class. A panel of eight clones reactive to both whole BTV and purified rVP7 protein were identified based on their reactivity in iELISA. For competitive ELISA,the clone designated as 4A10 showed better inhibition to hyperimmune serum of BTV serotype 23. However,this clone showed a variable percent of inhibition ranging from16.6% with BTV 12 serotype to 78.9% with BTV16 serotype using 24 serotype specific sera of BTV originating from guinea pig at their lowest dilutions. From the available panel of clones,only 4A10 was found to have a possible diagnostic application.

关键词： Bluetongue virus Competitive ELISA Enzyme-linked immunosorbent assay Monoclonal antibody India.

Detection of Bluetongue Virus Group-specific Antigen Using Monoclonal Antibody Based Sandwich ELISA

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Virologica Sinica 2010年第6期25卷 390-400页

作者： Pradeep narayan Gandhale Veerakyathappa Bhanuprakash Vinayagamurthy Balamurugan Madhusudhan Hosamani Gnanavel Venkatesan raj Kumar Singh Division of Virology Indian Veterinary Research Institute Project Directorate on Animal Disease Monitoring And Surveillance Indian Veterinary Research Institute National Research Centre on Equines

A monoclonal antibody (MAb) specific for the bluetongue virus (BTV) group specific antigen (VP7) was characterized for its reactivity with purified virus and recombinant BTV VP7 (rVP7) protein and its suitability for use in the sandwich *** MAb,designated as 5B5 was specific to VP7 and belongs to IgG2a subclass and was selected for the development of the sELISA in this *** MAb had a titer of 1:25 with BTV and 1:2 with the rVP7 *** sELISA is based on capturing of BTV antigen with VP7 specific MAb followed by detection using BTV polyclonal antiserum raised in *** assay was evaluated with six cell culture adapted serotypes of BTV that have been isolated from India,1,2,15,17,18 and *** assay could detect BTV antigen as early as day 8 in *** was also successfully applied for the detection of BTV group specific antigen in clinical samples of blood,washed RBCs,buffy coat and plasma.A total of 102 field samples from animals,suspected of being infected with BTV,were tested and 29.42% were *** blood samples were also amplified in cell culture which improved the sensitivity of the *** confirmed that the sELISA is rapid and specific.

关键词： Bluetongue Diagnosis ELISA Monoclonal antibody Sandwich ELISA

Influence of peach(Prunus persica Batsch)phenological stage on the short-term changes in oxidizable and labile pools of soil organic carbon and activities of carbon-cycle enzymes in the North-Western Himalayas

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Pedosphere 2020年第5期30卷 638-650页

作者： Sovan DEBNATH Brij Lai ATTRI Anil KUMAR Arun KISHOR raj narayan Kanchan SINHA Arpan BHOWMIK Anil SHARMA Desh Beer SINGH Indian Council of Agricultural Research(lCAR)-Central Institute of Temperate Horticulture Regional StationMukteshwarNainitalUttarakhand 263138(India) Department of Agricultural Chemistry and Soil Science Faculty of AgricultureBidhan Chandra Krishi ViswavidyalayaMohanpurWest Bengal 741252(India) Indian Council of Agricultural Research(ICAR)-Directorate of Mushroom Research SolanHimachal Pradesh 173213(India) A Indian Council of Agricultural Research(ICAR)-Indian Agricultural Statistics Research Institute New Delhi 110012(India) Department of Agricultural Statistics Faculty of AgricultureBidhan Chandra Krishi ViswavidyalayaMohanpurWest Bengal 741252(India) Indian Council of Agricultural Research(ICAR)-Central Institute of Temperate Horticulture SrinagarJammu and Kashmir 190007(India)

The labile organic carbon(C)and C-related enzymes are sensitive indicators capturing alterations of soil organic matter(SOM),even in a short-time *** the effects of crop husbandry and land use change on these attributes have been well studied,there is no consensus about how plant phenology may impact *** study aimed to determine the short-term effect o f six distinct phenological stages(PS-1:full bloom;PS-2:fruit set;PS-3:pit hardening;PS-4:physiological maturity;PS-5:60 d after physiological maturity;and PS-6:fall)o f peach on the changes in soil organic carbon(SOC)fractions of different oxidizability,labile C pools,and C-cycle enzyme activities in soils,for two consecutive years(2015 and 2016)in the North-Western Himalayas(NWH).Peach rhizosphere soils were sampled at the topsoil(0-15 cm)and subsoil(16-30 cm)layers,along with rhizosphere soils from adjacent perennial grasses,which served as a *** for most of the assessed parameters,including very labile C,labile C,microbial biomass C,permanganate oxidizable C,dissolved organic C,mineralizable C,amylase activity,and carboxymethyl-cellulase activity,were significantly(P<0.05)higher at PS-3 than at other phenological stages of ***,a sudden decline in these soil variables was recorded at PS-5,followed by a slight buildup at PS-6,particularly in the topsoil of the peach ***-term changes in organic C fractions of different oxidizability,influenced by peach phenological stage,significantly(P<0.05)affected C management index,C pool index,and lability *** the C management index and lability index showed their highest values at PS-3 and their lowest values at PS-5,clearly indicating short-term accretion and depletion of SOC,in tandem with the peach phenological *** component analysis suggested that a composite of soil indicators,including microbial biomass C,dissolved organic C,amylase,and invertase,could help detect short-term changes in SOC *** i

关键词： carbon fractions fruit orchard Indian Himalaya plant phenology rhizosphere soil soil biological indicators

Isolation and Identification of Potential Probiotic Bacteria from Cattle Farm Soil in Dibrugarh District

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Advances in Microbiology 2017年第4期7卷 265-279页

作者： Nuredin Mohamedkassm Siraj Kaushal Sood raj narayan Singh Yadav Asmara College of Health Sciences Asmara Eritrea Centre for Biotechnology and Bioinformatics Dibrugarh University Assam India Department of Life Sciences Dibrugarh University Assam India

Several studies have been done to isolate probiotic bacteria from different sources. In this present study, an attempt was made to isolate, screen and identify potential probiotic bacteria from cattle farm soil in Dibrugarh district, Assam, India. At the level of screening, the result showed the isolates designated as DUA4, DUD3 and DUE2 showed a percent survival rate of 75.36, 69.14 and 52.36 respectively at a pH of 2.5. Similarly survival rate of the same isolates in 0.5% bile salt condition was found to be 117.17%, 144.59% and 118.10% for the isolates DUA4, DUD3 and DUE2 respectively. Antimicrobial activity of the isolates towards the indicator organisms tested showed that DUA4 inhibited gram positive organisms while DUD3 showed activity against both gram positive and gram negative bacteria. All the three isolates showed activity against L. monocytogenes. Autoaggregation ability of the isolates DUA4, DUD3 and DUE2 was found to be 44.15%, 54.11% and 9.42% respectively. The adhesion ability of the isolates DUD3, DUA4 and DUE2 to xylene was 61.78%, 45.37% and 14.83% respectively. Antimicrobial susceptibility test of the isolates showed that the isolates are in general sensitive to antibiotics tested. Phenotypic and genotypic analysis based on the 16S rRNA gene sequence of the isolates DUA4, DUD3 and DUE2 resulted in the identification and designation of the isolate DUA4 as Bacillus spp., DUD3 as Enterococcus faecium and DUE2 as Enterobacter sp. In conclusion, the study has indicated the possibility of isolating potential probiotic bacterial strains from cattle farm soil.

关键词： Probiotic Soil Cattle Farm Enterococcus faecium Bacillus spp.

Investigation on phytochemical,antimicrobial activity and essential oil constituents of Nardostachys jatamansi *** different regions of Nepal

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Journal of Coastal Life Medicine 2016年第1期 56-60页

作者： narayan Sharma Amit raj Sharma Bishun D Patel Kanti Shrestha Department of Pharmaceutical Chemistry Doon Paramedical College and HospitalDehradunIndia Nepal Academy of Science and Technology(NAST) KhumaltarLalitpurNepal National Ayurveda Research and Training Centre KirtipurKathmanduNepal

Objective:To study chemical constituents of essential oil of the roots of Nardostachys jatamansi found in different regions of Nepal and also to investigate phytochemical as well as antimicrobial activity of the sample with high yield of essential ***:The essential oils of roots of plant from five different regions were extracted by hydro-distillation and analyzed by gas chromatography-mass spectroscopy for their chemical *** root samples were also subjected to hydro-alcoholic extraction and then fractionated with hexane,chloroform,n-butanol and water so as to perform phytochemical screening and antimicrobial ***:The essential oil yield of sample from Jumla was found to be the highest followed by a sample from Nepalgunj,Surkhet and Kathmandu whereas that of sample from Dharan was found to be the *** chromatography-mass spectrometer analysis of essential oil of five samples showed that“2-beta pinene”appeared dominated in three samples,namely,6VJ Nepalgunj,9VJA Jumla and 10VJ ***“alkohol aus neoclovenoxid”in 8VJ Dharan and“methoxy phenyloxime”in 13VJA Kathmandu was found to be present in the highest *** screening of different fractions of sample 9VJA Jumla showed the presence of alkaloids,terpenoids,glycosides,proteins and amino acids,and carbohydrates *** susceptibility test of same fractions showed the n-butanol fraction potent against all pathogens and most affected one was Escherichia ***:Our study suggests that the essential oil of Nardostachys jatamansi found in Nepal contains more than 80 compounds with their quality and quantity differing from place to place.

关键词： Antimicrobial susceptibility testing Essential oil GC-MS Nardostachys jatamansi Phytochemical screening

Innovative Practices for the Promotion of Local/Indigenous Knowledge for Disaster Risk Reduction Management in Sudur Paschim Province,Nepal

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Journal of Geographical Research 2021年第3期4卷 1-11页

作者： Kabi Prasad Pokhrel Shambhu Prasad Khatiwada narayan Prasad Paudyal Keshav raj Dhakal Chhabi Lal Chidi narayan Prasad Timilsena Dhana Krishna Mahat Central Department of Geography Education Tribhuvan UniversityKathmanduNepal Central Department of Geography Tribhuvan UniversityKathmanduNepal Central Department of Science Education Tribhuvan UniversityKathmanduNepal PhD Research Scholar of Geography Education Tribhuvan UniversityKathmanduNepal

This study is an attempt to point out the different types of natural hazards in Sudur Paschim province of Nepal where environmental degradation processes such as deforestation,desertification,biodiversity loss,soil crisp and watershed degradation are rising *** participatory method multi hazard prone areas were identified with type and *** criteria evaluation method was applied to prioritize replicable actions with location-specific innovative practices and their legitimization for integrating local and indigenous knowledge into mainstream education,science and policy with a view to incorporate local and indigenous knowledge as live science in disaster and climate change *** of the study reveal that varieties of natural hazards in combination with social factors such as poverty,conflict and inequality have resulted frequent disasters and social vulnerabilities in many parts of the ***,indigenous and local people have responded threats of multi hazards by using their traditional knowledge and skills which has evolved over generations,and continue to adapt to future *** traditional,often faith-based,beliefs and practices were found to use as the key to their resilience in the face of natural ***,many communities have been lost their knowledge as the elderly die without transferring it to younger *** this proper educational policy and strategic development plans have not been implemented to cope local/indigenous knowledge into *** of the study indicate that indigenous and local knowledge is a precious province resource that can support the process of disaster prevention,preparedness and response in cost-effective disaster risk ***,policy framework has to prime focus to integrate indigenous and local knowledge,wisdom and skills into mainstream educational programs in order to transfer science into policy and education(words)into practice.

关键词： Multihazard Indigenous knowledge Location-specific Innovative practice Indigenous communities Science to policy

Bone morphogenetic protein-4 affects both trophoblast and non-trophoblast lineage-associated gene expression in human embryonic stem cells

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Stem Cell Discovery 2012年第4期2卷 163-175页

作者： Margaret L. Shirley Alison Venable raj R. Rao Nolan L. Boyd Steven L. Stice David Puett Prema narayan Cardiovascular Innovation Institute University of Louisville Louisville USA Department of Biochemistry and Molecular Biology University of Georgia Athens USA Department of Chemical and Life Science Engineering School of Engineering Virginia Commonwealth University Richmond USA Department of Physiology Southern Illinois University School of Medicine Carbondale USA

Human embryonic stem cells (hESC) can be induced to differentiate to trophoblast by bone morphogenetic proteins (BMPs) and by aggregation to form embryoid bodies (EB), but there are many differences and controversies regarding the nature of the differentiated cells. Our goals herein were to determine if BG02 cells form trophoblast-like cells (a) in the presence of BMP4-plus-basic fibroblast growth factor (FGF-2) and (b) upon EB formation, and (c) whether the BMP4 antagonist noggin elicits direct effects on gene expression and hormone production in the cells. Transcriptome profiling of hESC incubated with BMP4/FGF-2 showed a down-regulation of pluripotency-associated genes, an up-regulation of trophoblast-associated genes, and either a down-regulation or no change in gene expression for many markers of the three embryonic germ layers. Yet, there was up-regulation of several genes associated with mesoderm, ectoderm, and endoderm, strongly suggesting that differentiation to trophoblast-like cells under the conditions used does not yield a homogeneous cell type. Several genes, heretofore unreported, were identified that are altered in hESC in response to BMP4-mediated differentiation. The production of human chorionic gonadotropin (hCG), progesterone, and estradiol in the differentiated cells confirmed that trophoblast-like cells were obtained. Gene expression by EB was characterized by an up-regulation of a number of genes associated with trophoblast, ectoderm, endoderm, and mesoderm, and the production of hCG and progesterone confirmed that trophoblast-like cells were formed. These results suggest that, in the presence of FGF-2, BG02 cells respond to BMP4 to yield trophoblast-like cells, which are also obtained upon EB formation. Thus, BMP4-mediated differentiation of hESC represents a viable cell system for studying early developmental events post-implantation;however, up-regulation of non-trophoblast genes suggests a somewhat diverse response to BMP4/FGF-2. Noggin a

关键词： Human Embryonic Stem Cells Trophoblasts Bone Morphogenetic Protein-4 Embryoid Bodies Noggin

Mitigating and managing COVID-19: promises and propositions from traditional health care practices in India and China

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History & Philosophy of Medicine 2021年第3期3卷 9-14页

作者： Sanjeev Rastogi raj Kamal Yadav Deep narayan Pandey Department of Kaya Chikitsa State Ayurvedic College and HospitalLucknow-226003India Department of AYUSH Govt of Uttar PradeshLucknowIndia Department of Environment/Forest Govt of RajasthanJaipurIndia

Whole world is currently facing the ongoing COVID-19 *** pandemic has affected globaleconomy,culture,societal structure,values and human psyche in an unprecedented *** review done through published researches in the relevant area proposes that it is highly imperative for traditional and modern health sciences to come together against ***-dences collected through practical experience,published research and classical health care wisdom spread through Chinese and Indian traditional health care texts set a point for integrative health care needs to manage *** are also suggestive of the supportive role of Ayurveda and TCM in COVID-19 mitigation and *** systems should therefore be fully explored for their potential as supportive care in on-going pandemic.

关键词： Ayurveda COVID-19 TCM Pandemic

RETRACTED: Innovative Practices for the Promotion of Local/Indigenous Knowledge for Disaster Risk Reduction Management in Sudur Paschim Province, Nepal

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Journal of Geographical Research 2022年第2期5卷 51-51页

This article has been *** original article has previously been published elsewhere without disclosure to the editor,permission to republish,or justification(i.e.,redundant publication).We apologise for any inconvenience this retraction may have caused *** to:RETRACTED:Innovative Practices for the Promotion of Local/Indigenous Knowledge for Disaster Risk Reduction Management in Sudur Paschim Province,Nepal Kabi Prasad Pokhrel,Shambhu Prasad Khatiwada,narayan Prasad Paudyal,Keshav raj Dhakal,Chhabi Lal Chidi,narayan Prasad Timilsena,Dhana Krishna Mahat Journal of Geographical Research,Volume 4,Issue 3,July 2021.

关键词： Local editor redundant