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Molecular Mechanism of Electroacupuncture Regulating Cerebral Arterial Contractile Protein in Rats with Cerebral Infarction Based on MLCK Pathway

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Chinese Journal of Integrative Medicine 2023年第1期29卷 61-68页

作者： LI Jing ZHANG Min HE Ying DU Yuan-hao ZHANG Xue-zhu rainer georgi Bernhard Kolberg Xu Yan-long Institute of Acupuncture and Moxibustion First Teaching Hospital of Tianjin University of Traditional Chinese MedicineNational Clinical Research Center for Chinese Medicine Acupuncture and MoxibustionTianjin300193China Max Planck Institute for Medical Research Heidelberg69120Germany Uniklinik Mannheim Mannheim68167Germany Department of Acupuncture and Moxibustion Gansu Provincial Hospital of Traditional Chinese MedicineLanzhou730050China

Objective To explore the effect of electroacupuncture(EA)intervention on the vasoconstriction of cerebral artery smooth muscle cells after cerebral *** Male Wistar rats were randomly divided into 3 groups by a random number table:the model group(n=24),the EA group(n=24),and the normal group(n=6).The model and the EA groups were divided into different time subgroups at 0.5,1,3,and 6 h after middle cerebral artery occlusion(MCAO),with 6 rats in each *** model was established using intraluminal suture occlusion *** EA group was given EA treatment at acupoint Shuigou(GV 26)instantly after MCAO for 20 *** contents of cerebrovascular smooth muscle MLCK,the 3 subunits of myosin light chain phosphatase(MLCP)MYPT1,PP1c-δand M20,as well as myosin-ATPase activity were detected using immunohistochemistry and Western *** The overall expression level of the MYPT1 and PP1c-δin the model group was significantly higher(P0.05),and the other subgroups were still significantly higher than the normal group(P<0.01).After EA intervention,the expression level of each subgroup was significantly lower than the corresponding model *** was a significant difference between the 0.5 and 1 h subgroups(P<0.01),while a difference was also observed between the 3 and 6 h subgroups(P<0.05).The dynamic change rule gradually increased with the prolongation of infarction time within 6 h after *** EA intervention can inhibit contraction of cerebral vascular smooth muscle cells and regulate smooth muscle relaxation by regulating MLCK pathway.

关键词： infarction vasomotor MLCK pathway Shuigou(GV 26) electroacupuncture

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Effect of Electro-acupuncture on Vasomotor Symptoms in Rats with Acute Cerebral Infarction Based on Phosphatidylinositol System

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Chinese Journal of Integrative Medicine 2022年第2期28卷 145-152页

作者： LI Jing HE Ying DU Yuan-hao ZHANG Min rainer georgi Bernhard Kolberg SUN Dong-wei MA Kun LI Yong-feng ZHANG Xue-zhu Key Laboratory of Acupuncture of Tianjin First Teaching Hospital of Tianjin University of Traditional Chinese MedicineNational Clinical Research Center for Chinese Medicine Acupuncture and MoxibustionTianjin(300381)China Department of Acupuncture and Moxibustion First Teaching Hospital of Tianjin University of Traditional Chinese MedicineTianjin(300381)China Department of Chemical Biology Max Planck Institute for Medical ResearchHeidelberg(69120)Germany Department of Internal Medicine Mannheim Medical School of Heidelberg UniversityMannheim(68167)Germany Department of Chinese Medicine Rehabilitation Shenzhen Baoan District Hospital of Traditional Chinese MedicineShenzhenGuangdong Province(518000)China Department of Preventive Treatment of Disease Binhai New Area Hospital of Traditional Chinese MedicineFourth Teaching Hospital of Tianjin University of Traditional Chinese MedicineTianjin(300193)China Institute of Acupuncture and Moxibustion Shaanxi University of Chinese MedicineXi'an(712046)China

Objective:To investigate the effect of electro-acupuncture(EA)on vasomotor symptoms in rats with acute cerebral infarction,by observing the changes in the expression of factors related to the phosphatidylinositol(PI)***:Forty-two Wistar rats were randomly divided into 3 groups by a random number table:the control group(n=6),the model group(n=18)and the EA group(n=18).The EA group was given EA treatment at Shuigou(GV 26)instantly after modeling with middle cerebral artery occlusion(MCAO)method,while the model and control groups were not given any *** degrees of neurological deficiency were evaluated using neurological severity scores(NSS)and the brain blood flow was evaluated by a laser scanning confocal *** blot analysis was conducted to detect the expression levels of G-protein subtype(Gq)and calmodulin(CaM).Competition for protein binding was conducted to detect the expression level of inositol triphosphate(IP3).Thin layer quantitative analysis was conducted to detect the expression level of diacylglycerol(DAG).The expression level of intracellular concentration of free calcium ion([Ca^(2+)]i)was detected by flow ***:The NSS of the model group was significantly higher than the control group at 3 and 6 h after MCAO(P<0.01),while the EA group was significantly lower than the model group at 6 h(P<0.01).The cerebral blood flow in the model group was significantly lower than the control group at 1,3 and 6 h after MCAO(P<0.01),while for the EA group it was remarkably higher than the model group at the same time points(P<0.01).The expressions of Gq,CaM,IP3,DAG and[Ca^(2+)]i in the model group were significantly higher than the control group(P<0.05 or P<0.01),and those in the EA group were significantly lower than the model group at the same time points(P<0.05 or P<0.01).Conclusion:EA treatment at GV 26 can effectively decrease the over-expression of related factors of PI system in rats with acute cerebral infarction,impro

关键词： cerebral infarction phosphatidylinositol system vasomotion Shuigou(GV 26) electro-acupuncture Chinese medicine

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针刺对脑梗死大鼠缺血脑组织血管新生相关因子表达的影响

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Acupuncture and Herbal Medicine 2023年第1期3卷 46-54页

作者：孙雅洁李晶 rainer georgi Bernhard Kolberg 杨丽红天津中医药大学第一附属医院天津300193 国家中医针灸临床医学研究中心天津300193 Department of Chemical Biology Max Planck Institute for Medical ResearchGermany Department of Internal Medicine Mannheim Medical School of Heidelberg UniversityGermany

目的:动态研究脑梗死后血管新生相关因子的变化规律和针刺干预效应,从促进血管新生角度深入揭示针刺治疗脑梗死的分子机制。方法:以MCAO模型大鼠为研究对象,分为针刺组、模型组和空白组。观察脑梗死后神经功能缺损程度改变,同时采用免... 详细信息

目的:动态研究脑梗死后血管新生相关因子的变化规律和针刺干预效应,从促进血管新生角度深入揭示针刺治疗脑梗死的分子机制。方法:以MCAO模型大鼠为研究对象,分为针刺组、模型组和空白组。观察脑梗死后神经功能缺损程度改变,同时采用免疫荧光双标记法观察缺血中心及边缘区的血管新生情况,应用RT-PCR和Western blot法检测脑梗死后缺穴区域血管新生相关因子Ang-1、Ang-2、PDGF-B和bFGF mRNA及蛋白变化规律和电针干预效应。结果:电针组各时相NSS评分均小于同时相模型组。MCAO后9h~7d时电针组血管内皮细胞增殖高于同时相模型组。电针组Ang-1、Ang-2 mRNA及蛋白表达水平明显高于模型组。电针组PDGF-B mRNA在3h~6h、3d~12d时明显高于模型组,蛋白水平在6h、3d~12d时明显高于模型组。针刺组bFGF mRNA在24h~12d、蛋白水平在3d~12d时明显高于模型组。结论:电针水沟穴能明显改善MCAO大鼠神经功能症状;能够促进MCAO模型大鼠梗死区周围血管内皮细胞增殖,并将血管内皮细胞增殖出现时间显著提前,其可能机制为电针水沟穴可上调血管新生相关因子的表达,并使表达时相前移,从而促进血管新生,对改善脑梗死的预后起到了明显的积极作用。

关键词：人中穴脑梗死电针血管新生

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针刺对脑梗死大鼠缺血脑组织血管新生相关因子表达的影响（英文）

引用

Acupuncture and Herbal Medicine 2023年第1期 46-54页

作者：孙雅洁李晶 rainer georgi Bernhard Kolberg 杨丽红天津中医药大学第一附属医院国家中医针灸临床医学研究中心 Department of Chemical Biology Max Planck Institute for Medical Research Department of Internal Medicine Mannheim Medical School of Heidelberg University

目的：动态研究脑梗死后血管新生相关因子的变化规律和针刺干预效应，从促进血管新生角度深入揭示针刺治疗脑梗死的分子机制。方法：以MCAO模型大鼠为研究对象，分为针刺组、模型组和空白组。观察脑梗死后神经功能缺损程度改变，同时采... 详细信息

目的：动态研究脑梗死后血管新生相关因子的变化规律和针刺干预效应，从促进血管新生角度深入揭示针刺治疗脑梗死的分子机制。方法：以MCAO模型大鼠为研究对象，分为针刺组、模型组和空白组。观察脑梗死后神经功能缺损程度改变，同时采用免疫荧光双标记法观察缺血中心及边缘区的血管新生情况，应用RT-PCR和Western blot法检测脑梗死后缺穴区域血管新生相关因子Ang-1、Ang-2、PDGF-B和b FGF m RNA及蛋白变化规律和电针干预效应。结果：电针组各时相NSS评分均小于同时相模型组。MCAO后9h～7d时电针组血管内皮细胞增殖高于同时相模型组。电针组Ang-1、Ang-2 m RNA及蛋白表达水平明显高于模型组。电针组PDGF-B m RNA在3h～6h、3d～12d时明显高于模型组，蛋白水平在6h、3d～12d时明显高于模型组。针刺组b FGF m RNA在24h～12d、蛋白水平在3d～12d时明显高于模型组。结论：电针水沟穴能明显改善MCAO大鼠神经功能症状；能够促进MCAO模型大鼠梗死区周围血管内皮细胞增殖，并将血管内皮细胞增殖出现时间显著提前，其可能机制为电针水沟穴可上调血管新生相关因子的表达，并使表达时相前移，从而促进血管新生，对改善脑梗死的预后起到了明显的积极作用。

关键词：人中穴脑梗死电针血管新生

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食管肿瘤X线诊断的现状

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放射学实践 1992年第3期7卷 93-95页

作者： Max georgi Hans-Peter Busch rainer Simon Andre Sommer Eduard Wetzel 王天才 Institut fur Klinische Radiologie des Klinikums Mannheim Th.-Katzer-Ufer6800 MannheimFRG.Prof 同济医科大学附属同济医院

传统X线检查方法能及时、无痛苦地诊断吞咽困难。X线诊断应在内窥镜检查前进行,因其病理结果对内窥镜的施行具有参考意义。低张双对比造影是一种重要的补充,而CT和磁共振对于术前肿瘤分期有重要意义。

关键词：食管肿瘤 X线诊断

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