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Role of HSP-90 for increased nNOS-mediated vasodilation in mesenteric arteries in portal hypertension

World Journal of Gastroenterology 2010年第15期16卷 1837-1844页

作者： Lukas Moleda Lars Jurzik Matthias Froh Erwin Gbele Claus Hellerbrand rainer H Straub Jürgen Schlmerich Reiner Wiest Department of Internal Medicine I University Medical Center

AIM:To explore the role of heat shock protein-90 (HSP-90) for nitrergic vasorelaxation in the splanchnic circulation in rats with and without portal hypertension. METHODS: Neuronal nitric oxide synthase (nNOS) and HSP-90 were analyzed by immunofluorescence, western blotting and co-immunoprecipitation in the mesenteric vasculature and isolated nerves of portal-vein-ligated (PvL) rats and sham operated rats. In vitro perfused de-endothelialized mesenteric arterial vasculature was preconstricted with norepinephrine (EC80) and tested for nNOS-mediated vasorelaxation by periarterial nerve stimulation (PNS, 2-12 Hz, 45v) before and after incubation with geldanamycin (specific inhibitor of HSP-90 signalling, 3 μg/mL) or L-NAME (non-specific NOSblocker, 10-4 mol/L). RESULTS: nNOS and HSP-90 expression was significantly increased in mesenteric nerves from PvL as compared to sham rats. Moreover, nNOS and HSP-90 were visualized in mesenteric nerves by immunofluorescence and immunoprecipitation of nNOS co-immunoprecitated HSP-90 in sham and PvL rats. PNS induced a frequencydependent vasorelaxation which was more pronounced in PvL as compared to sham rats. L-NAME and geldanamycin markedly reduced nNOS-mediated vasorelaxation abrogating differences between the study groups. The effect of L-NAME and geldanamycin on nNOS-mediated vasorelaxation was significantly greater in PvL than in sham animals. However, no difference in magnitude of effect between L-NAME and geldanamycin was noted. CONCLUSION: HSP-90 acts as a signalling mediator of nNOS-dependent nerve mediated vascular responses in mesenteric arteries, and the increased nitrergic vasorelaxation observed in portal hypertension is mediated largely by HSP-90.

关键词： Heat shock protein-90 Nitric oxide vasodilation Portal hypertension Mesenteric circulation

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Surgical experience in splitting donor liver into left lateral and right extended lobes

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World Journal of Gastroenterology 2005年第27期11卷 4220-4224页

作者： Ji-Qi Yan Thomas Becker Michael Neipp Cheng-Hong Peng rainer Lueck Frank Lehner Hong-Wei Li Juergen Klempnauer Department of Surgery Ruijin Hospital Affiliated to Shanghai Second Medical University Shanghai 200025 China Department of Abdominal and Transplant Surgery Medical School of Hannover Carl-NeubergStr. 1 Hannover 30625 Germany

AIM： To outline the surgical experience with donor liver splitting in split liver ***： From March 1 to September 1 in 2004, 10 donor livers were split ex situ into a left lateral lobe （segments Ⅱ and Ⅲ） and a right extended lobe （segments Ⅰ, Ⅳ-Ⅷ） in Medical School of Hannover, and thereafter split liver transplantation was performed successfully in 29 cases. The average age, weight and ICU slaying period of the donors were 32.7 years （25-52 years）, 64.5 kg（45-75 kg） and 2.4 d （2-8 d） ***： The average weight of the whole graft and the left lateral lobe was 2 322.6 g （956-2 665 g） and 282.8 g （298-373 g） respectively, and the average ratio of left lateral lobe to the whole graft was 0.225 （0.278-0.274）.The average graft to recipient weight ratio （GRWR） of the left lateral lobe and the right extended lobe reached 2.44% （2.22-5.42%） and 2.73% （2.32-2.30%） *** average it took approximately 205 min （85-235 rain）to split the donor liver. Five donor organs showed anatomic variation including the left hepatic vein variation in two cases, the left hepatic artery variation in two cases and the bile duct variation in one ***： Split liver transplantation has become a mature surgical technique to expand the donor pool with promising results. In the process of graft splitting, close attention needs to be paid to potential anatomic variations,especially to variations of the left hepatic vein, the left hepatic artery, and the bile duct.

关键词： Split Donor liver Anatomic variation

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Determination of glycated hemoglobin in patients with advanced liver disease

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World Journal of Gastroenterology 2004年第15期10卷 2284-2286页

作者： Theresa Lahousen Karin Hegenbarth Rottraut Ille rainer W.Lipp Robert Krause Randie R. Little Wolfgang J.Schnedl Department of Psychiatry School of MedicineMedical University GrazAuenbruggerplatz 31A-8063 GrazAustria Department of Internal Medicine School of MedicineMedical University GrazAuenbruggerplatz 15A-8063 GrazAustria University of Missouri School of Medicine Department of Pathology and Child HealthOne Hospital DriveColumbiaMO 65212USA

AIM:To evaluate the glycated hemoglobin(HbA_(1c)) determination methods and to determine fructosamine in patients with chronic hepatitis,compensated cirrhosis and in patients with chronic hepatitis treated with ribavirin. METHODS:HbA_(1c) values were determined in 15 patients with compensated liver cirrhosis and in 20 patients with chronic hepatitis using the ion-exchange high performance liquid chromatography and the immunoassay methods. Fructosamine was determined using nitroblue tetrazolium. RESULTS:Forty percent of patients with liver cirrhosis had HbA_(1c) results below the non-diabetic reference range by at least one HbA_(1c)method,while fructosamine results were either within the reference range or *** percent of patients with chronic hepatitis(hepatic fibrosis) had HbA_(1c)results below the non-diabetic reference range by at least one HbA_(1c)*** patients with chronic hepatitis treated with ribavirin,50% of HbA_(1c)results were below the non-diabetic reference using at least one of the HbA_(1c)methods. CONCLUSION:Only evaluated in context with all liver function parameters as well as a red blood count including reticulocytes,HbA_(1c)results should be used in patients with advanced liver ***_(1c)and fructosamine measurements should be used with caution when evaluating long-term glucose control in patients with hepatic cirrhosis or in patients with chronic hepatitis and dbavidn treatment.

关键词： Antiviral Agents Fructosamine Hemoglobin A, Glycosylated Hepatitis C, Chronic Hepatitis, Chronic Humans Liver Cirrhosis Ribavirin

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用掠入射小角X射线散射（GISAXS）研究在退火作用下乳胶膜表面结构的演化及重组

用掠入射小角X射线散射（GISAXS）研究在退火作用下乳胶膜表面结...

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2007年全国高分子学术论文报告会

作者：胡珊珊 Jens Riege Stephan v.Roth rainer Gehrke 门永锋中国科学院长春应用化学研究所高分子物理和化学国家重点实验室 BASF Aktiengesellschaft Polymer Physics 67056 LudwigshafenGermany HASYLAB am DESY Notkestr.85 D-22607 HamburgGermany HASYLAB am DESY Notkestr.85 D-22607 HamburgGermany 高分子物理和化学国家重点实验室中国科学院长春应用化学研究所

＜正＞具有壳核结构的乳胶粒子（R=250nm）在硅片上干燥成膜后在不同温度下退火,其表面结构发生一系列演变。这一过程用掠入射小角 X 射线散射（GISAXS）和原子力显微镜（AFM）的方法对其进行了研究。体积百分数为1％的乳胶液（核/壳... 详细信息

＜正＞具有壳核结构的乳胶粒子（R=250nm）在硅片上干燥成膜后在不同温度下退火,其表面结构发生一系列演变。这一过程用掠入射小角 X 射线散射（GISAXS）和原子力显微镜（AFM）的方法对其进行了研究。体积百分数为1％的乳胶液（核/壳：聚苯乙烯/聚丙烯酸酯）

关键词：掠入射小角X射线散射 GISAXS 乳胶表面退火

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Chronic hepatitis C:Treat or wait? Medical decision making in clinical practice

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World Journal of Gastroenterology 2012年第12期18卷 1339-1347页

作者： Claus Niederau Dietrich Hüppe Elmar Zehnter Bernd Mller Renate Heyne Stefan Christensen rainer Pfaff Arno Theilmeier Ulrich Alshuth Stefan Mauss Katholische Kliniken Oberhausen St.Josef HospitalKlinik für Innere MedizinOberhausen 46045Germany Medical Office for Gastroenterology and Hepatology Herne 44623Germany Medical Office for Gastroenterology and Hepatology Dortmund 44263Germany Medical Office for Gastroenterology and Hepatology Berlin 10969Germany Center for Interdisciplinary Medicine Münster 48143Germany Medical Office for Gastroenterology and Hepatology Gie en 35392Germany Medical Office for Gastroenterology and Hepatology Mnchengladbach 41239Germany Pharma AG Medical Management virology Grenzach-Wyhlen 79639Germany Center for HIv and Hepatogastroenterology Düsseldorf 40237Germany

AIM:To analyzes the decision whether patients with chronic hepatitis C virus (HCv) infection are treated or ***:This prospective cohort study included 7658 untreated patients and 6341 patients receiving pegylated interferon α2 a/ribavirin,involving 434 physicians/institutions throughout Germany (377 in private practice and 57 in hospital settings).A structured questionnaire had to be answered prior to the treatment decision,which included demographic data,information about the personal life situation of the patients,anamnesis and symptomatology of hepatitis C,virological data,laboratory data and data on concomitant diseases.A second part of the study analyzes patients treated with pegylated interferon α*** questionnaires included reasons against treatment mentioned by the ***:Overall treatment uptake was 45%.By multivariate analysis,genotype 1/4/5/6,HCv-RNA ≤ 520 000 IU/mL,normal alanine aminotransferase (ALT),platelets ≤ 142 500/μL,age > 56 years,female gender,infection length > 12.5 years,concomitant diseases,human immunodeficiency virus co-infection,liver biopsy not performed,care in private practice,asymptomatic disease,and unemployment were factors associated with reduced treatment *** and sustained viral response rates in migrants (1/3 of cohort) were higher than in German natives although 1/3 of migrants had language *** rate and liver biopsy were higher in clinical settings when compared to private practice and were low when ALT and HCv-RNA were ***:Some reasons against treatment were medically based whereas others were related to fears,socio-economical problems,and information deficits both on the side of physicians and patients.

关键词： Hepatitis C virus Interferon, Ribavirin Livercirrhosis Migrants Treatment barrier

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流程设备的安全保护方法

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流程工业 2020年第1期 40-40,42页

作者： rainer Semmler Christian Eberle Tüv

用适用性验证来取代失效概率,学会正确选择流程设备的安全保护方法——以蝶阀、泄压阀和防爆盘为例,必须用不同于电子安全保护装置的方法,来考察机械保护装置的安全性能。

关键词：安全保护装置泄压阀失效概率安全性能选择流程设备的安全适用性验证机械保护装置

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Methods, units and quality requirements for the analysis of haemoglobin A_(1c) in diabetes mellitus

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World Journal of Methodology 2016年第2期6卷 133-142页

作者： Ilkka Penttil? Karri Penttil? P?ivi Holm Harri Laitinen P?ivi Ranta Jukka T?rr?nen rainer Rauramaa Kuopio Research Institute of Exercise Medicine University of Eastern Finland Finnish Medicines Agency FIMEA Fimlab Laboratories Ltd. Labquality Ltd.

The formation of glycohemoglobin, especially the hemoglobin A1c(Hb_(A1c)) fraction, occurs when glucose becomes coupled with the amino acid valine in the β-chain of Hb; this reaction is dependent on the plasma concentration of glucose. Since the early 1970 s it has been known that diabetics display higher values of Hb_(A1c) because they have elevated blood glucose concentrations. Thus Hb_(A1c) has acquired a very important role in the treatment and diagnosis of diabetes mellitus. After the introduction of the first quantitative measurement of Hb_(A1c), numerous methods for glycohemoglobin have been introduced with different assay principles: From a simple minicolumn technique to the very accurate automated highpressure chromatography and lastly to many automated immunochemical or enzymatic assays. In early days, the results of the quality control reports for Hb_(A1c) varied extensively between laboratories, therefore in United States and Canada working groups(WG) of the Diabetes Controls and Complications Trial(DCCT) were set up to standardize the Hb_(A1c) assays against the DCCT/National Glycohemoglobin Standardization Program reference method based on liquid chromatography. In the 1990 s, the International Federation of Clinical Chemistry and Laboratory Medicine(IFCC) appointed a new WG to plan a reference preparation and method for the Hb_(A1c) measurement. When the reference procedureswere established, in 2004 IFCC recommended that all manufacturers for equipment used in Hb_(A1c) assays should calibrate their methods to their proposals. This led to an improvement in the coefficient of variation(Cv%) associated with the assay. In this review, we describe the glycation of Hb, methods, standardization of the Hb_(A1c) assays, analytical problems, problems with the units in which Hb_(A1c) values are expressed, reference values, quality control aspects, target requirements for Hb_(A1c), and the relationship of the plasma glucose values to Hb_(A1c) concentrations. We a

关键词： Diabetes Hemoglobin A1c Glycohemoglobin Glucose International Federation of Clinical Chemistry and Laboratory Medicine Reference values Quality assurance Recommendation Target limits

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Copper as an alternative antimicrobial coating for implants-An in vitro study

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World Journal of Transplantation 2017年第3期7卷 193-202页

作者： Claudia Bergemann Sarah Zaatreh Katharina Wegner Kathleen Arndt Andreas Podbielski rainer Bader Cornelia Prinz Ulrich Lembke J Barbara Nebe Department of Cell Biology University Medical Center Rostock Biomechanics and Implant Technology Research Laboratory University Medical Center Rostock Institute of Medical Microbiology Virology and HygieneUniversity Medical Center Rostock DOT GmbH

AIM To investigate osteoconductive and antimicrobial properties of a titanium-copper-nitride(TiC uN) film and an additional BONIT~? coating on titanium *** For micro-structuring, the surface of titanium test samples was modified by titanium plasma spray(TPS). On the TPS-coated samples, the Ti Cu N layer was deposited by physical vapor deposition. The BONIT~? layer was coated electrochemically. The concentration of copper ions released from TiC uN films was measured by atomic absorption spectrometry. MG-63 osteoblasts on Ti Cu N and BONIT~? were analyzed for cell adhesion, viability and spreading. In parallel, Staphylococcus epidermidis(S. epidermidis) were cultivated on the samples and planktonic and biofilm-bound bacteria were quantified bycounting of the colony-forming units. RESULTS Field emission scanning electron microscopy(FESEM) revealed rough surfaces for TPS and TiC uN and a special crystalline surface structure on TiC uN + BONIT~?. TiC uN released high amounts of copper quickly within 24 h. These release dynamics were accompanied by complete growth inhibition of bacteria and after 2 d, no planktonic or adherent S. epidermidis were found on these samples. On the other hand viability of MG-63 cells was impaired during direct cultivation on the samples within 24 h. However, high cell colonization could be found after a 24 h pre-incubation step in cell culture medium simulating the in vivo dynamics closer. On pre-incubated TiC uN, the osteoblasts span the ridges and demonstrate a flattened, well-spread phenotype. The additional BONIT~?-coating reduced the copper release of the TiC uN layer significantly and showed a positive effect on the initial cell *** The Ti Cu N-coating inhibits the formation of bacterial biofilms on orthopedic implants by influencing the "race for the surface" to the advantage of osteoblasts.

关键词： Implant-coating Antimicrobial effect Titanium plasma spray Titanium-copper-nitride BONIT^(®) Osteoconductivity

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L'Orange公司平台设计的共轨式高压泵

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国外内燃机车 2005年第4期 14-23页

作者： rainer W.Jorach 韩树明 (德)

1997年8月,LOrange公司研制出世界上第一套电子控制的共轨式燃油喷射系统。该系统在MTU/DDC4000系列柴油机上的应用使该柴油机取得极大的成功。该喷射系统使燃烧系统面貌一新,在燃油效率、排放和烟度以及输出功率和扭矩等方面均有极大... 详细信息

1997年8月,LOrange公司研制出世界上第一套电子控制的共轨式燃油喷射系统。该系统在MTU/DDC4000系列柴油机上的应用使该柴油机取得极大的成功。该喷射系统使燃烧系统面貌一新,在燃油效率、排放和烟度以及输出功率和扭矩等方面均有极大的改进。柴油机发展的前一百年的特点大体上只体现在功率输出和燃油效率方面的改进。将来的一个主要任务将是满足排放法规的要求,这些要求将愈来愈苛刻,差不多每3至7年变更一次。对于高压油泵,存在一种很大的潜力,可以仅用一种型号或一种系列的油泵来满足几种发动机机型的需要,这可以使费用保持在可接受的水平。这些基于平台设计的油泵可导致柴油机在配件供应、经济性以及运行性能方面的改进。LOrange公司在2002年为20缸v型长行程MTU4000系列柴油机研制的直列式高压泵就是带锐边的柱塞泵技术的第一个实例,本文将对此详细介绍。令人瞩目的是,同一型号的直列式泵可适用于整个系列的柴油机,从4缸100kW/缸(采用一台泵)到18缸1200kW/缸的v型机(采用4台泵),使柴油机结构显著简化。泵的可用转速由800r/min到3150r/min,采用由柴油机曲轴驱动的齿轮,选用适当的齿轮比,从而克服了泵转速对各种柴油机标定转速的依赖性。目前该泵有2种柴油型的:一种是4柱塞型的;另一种是2柱塞型的,供油量分别为32L/min和16L/min。这些基于平台设计的油泵的普遍选择将为开发人员提供这样的机会:使喷射系统和燃烧系统的开发更集中在发动机性能、应用和系统的集成化上,而不只是停留在新的零部件的开发上。

关键词： L’Orange公司柴油机共轨式高压泵燃油喷射机车共轨技术

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Distribution and effects of polymorphic RANTES gene alleles in HIv/HCv coinfection - A prospective cross-sectional study

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World Journal of Gastroenterology 2005年第48期11卷 7631-7638页

作者： Golo Ahlenstiel Agathe Iwan Jacob Nattermann Karin Bueren Jürgen K Rockstroh Hans H Brackmann Bernd Kupfer Olfert Landt Amnon Peled Tilman Sauerbruch Ulrich Spengler rainer P Woitas Department of Internal Medicine 1 Institute of Experimental Hematology Institute of Medical Microbiology and Immunology TIB MOLBIOL Synthesis Laboratory Goldyne Savad Institute of Gene Therapy

AIM: Chemokines and their receptors are crucial for immune responses in HCv and HIv infection. RANTES gene polymorphisms lead to altered gene expression and influence the natural course of HIv infection. Therefore,these mutations may also affect the course of HIv/HCv ***: We determined allele frequencies of RANTES-403 (G→A), RANTES-28 (C→G) and RANTESIN1.1 (T→C) polymorphisms using real-time PCR and hybridization probes in patients with HIv (n = 85), HCv (n= 112), HIv/HCv coinfection (n = 121), and 109 healthy controls. Furthermore, HIv and HCv loads as well as CD4+ and CD8+ cell counts were compared between different RANTES ***: Frequencies of RANTES-403 A, RANTES-28 G and RANTES-IN1.1 C alleles were higher in HIv infected patients than in healthy controls (-403: 28.2% vs 15.1%,P = 0.002; -28: 5.4% vs 2.8%, not significant; IN1.1:19.0% vs 11.0%, P = 0.038). In HIv/HCv coinfected patients, these RANTES alleles were less frequent than in patients with HIv infection alone (15.4% P = 0.002;1.7%; P = 0.048; 12.0%; not significant). Frequencies of these alleles were not significantly different between HIv/HCv positive patients, HCv positive patients and healthy ***: All three RANTES polymorphisms showed increased frequencies of the variant allele exclusively in patients with HIv monoinfection. The finding that the frequencies of these alleles remained unaltered in HIv/HCv coinfected patients suggests that HCv coinfection interferes with selection processes associated with these alleles in HIv infection.

关键词： RANTES polymorphism HIv/HCv-coinfectJon HCv

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