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Biochar influences the cane fields’microbiota and the development of pre-sprouted sugarcane seedlings

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Waste Disposal and Sustainable Energy 2023年第1期5卷 75-88页

作者： Bruna Cristina Pinto Adriana Barboza Alves Osania Emerenciano ferreira Gustavo henrique Gravatim Costa Magno Andréde Oliveira Augusto Cesar da Silva Bezerra Alan rodrigues Teixeira Machado Postgraduate Program in Environmental Sciences State University of Minas GeraisFrutal UnityFrutalMinas Gerais38202-436Brazil Department of Agricultural and Biological Sciences State University of Minas GeraisFrutal UnityFrutalMinas Gerais38208-436Brazil Postgraduate Program in Geotechnical Engineering Federal University of Ouro PretoOuro PretoMinas Gerais35400-000Brazil Department of Transport Engineering Federal Center for Technological Education of Minas GeraisBelo HorizonteMinas Gerais30421-169Brazil Department of Exact Sciences State University of Minas GeraisJoão Monlevade UnityJoão MonlevadeMinas Gerais35930-314Brazil

This research investigates the soil conditioning effect of sugar cane fields with biochar produced from sugarcane *** influence on the physicochemical and microbiological conditions of the soil and the agronomic performance of sugarcane seedlings was *** this end,the biochar from sugarcane bagasse was produced by pyrolysis in a double drum oven and mixed with soil at rates of 0,1%,3%,and 5%(in weight).The experiment was installed in a greenhouse with two evaluation periods(30 days and 60 days)and five *** pot received a pre-sprouted cane seedling,and the analysis of colony-forming units(bacteria and fungi),physicochemical characteristics of the soil,and growth and development of sugarcane were performed per *** biochar showed a high fixed carbon content(72%)and a specific surface area estimated by the adsorption of methylene blue of 50 m2 g−***,its structure is porous and contains important nutrients(e.g.,CaO,K2O,and P2O5).In the periods evaluated,the applications of the 3%and 5%(in weight)rates of biochar reduced most of the soil fertility ***,the chemical analyses indicated that the sugarcane field soil collected already possessed high *** microbiota was influenced,but only the application of the 3%(in weight)rate at 60 days after planting showed a significant positive effect on the number of bacteria forming units,with an increase of approximately 385%.On the other hand,no significant positive effect on sugarcane development was ***,biochar application in high-fertility sugarcane plantation soil did not result in gains for sugarcane development in the periods evaluated.

关键词： Soil Biometry Microbiology Double drum oven Biochar

Near-infrared leaf reflectance modeling of Annona emarginata seedlings for early detection of variations in nitrogen concentration

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Journal of Forestry Research 2023年第1期34卷 269-282页

作者： Rafaela Lancas Gomes Marília Caixeta Sousa Felipe Girotto Campos Carmen Sílvia Fernandes Boaro José raimundo de Souza Passos Gisela ferreira Bioestatistic Plant BiologyParasitology and Zoology DepartmentBioscience Institute(IBB)Universidade Estadual Paulista(UNESP)BotucatuSao PauloBrazil

Nitrogen(N)monitoring is essential in nurseries to ensure the production of high-quality *** spectroscopy(NIRS)is an instantaneous,nondestructive method to monitor *** data such as NIRS can also provide the basis for developing a new vegetation spectral index(VSI).Here,we evaluated whether NIRS combined with statistical modeling can accurately detect early variations in N concentration in leaves of young plants of Annona emargiaata and developed a new VSI for this *** were grown in a hydroponics system with 0,2.75,5.5or 11 mM N for 45 *** we measured gas exchange,chlorophylla fluorescence,and pigments in leaves;analyzed complete leaf nutrients,and recorded spectral data for leaves at 966 to 1685 nm using *** a statistical learning approach,the dimensionality of the spectral data was reduced,then models were generated using two classes(N deficiency,N)or four classes(0,2.75,5.5,11 mM N).The best combination of techniques for dimensionality reduction and classification,respectively,was stepwise regression(PROC STEPDISC)and linear discriminant *** was possible to detect N deficiency in seedlings leaves with 100%precision,and the four N concentrations with93.55%accuracy before photosynthetic damage to the plant ***,NIRS combined with statistical modeling of multidimensional data is effective for detecting N variations in seedlings leaves of ***.

关键词： Mineral nutrition of plants Near-infrared spectroscopy Spectral vegetation index Digital signature Statistical learning Fluorescence of chlorophylla

Study of tumor necrosis factor receptor in the inflammatory bowel disease

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World Journal of Gastroenterology 2023年第18期29卷 2733-2746页

作者： Roberta Figueiroa Souza Marcos Antônio ferreira Caetano henrique Inhauser Riceti Magalhães Patricia Castelucci Department of Anatomy Institute of Biomedical SciencesUniversity of São PauloSão Paulo 05508-000Brazil Department of Surgery School of Veterinary Medicine and Animal SciencesUniversity of São PauloSão Paulo 05508-270Brazil

Ulcerative colitis(UC)and Crohn’s disease(CD)are part of Inflammatory Bowel Diseases(IBD)and have pathophysiological processes such as bowel necrosis and enteric neurons and enteric glial *** addition,the main inflammatory mediator is related to the tumor necrosis factor-alpha(TNF-α).TNF-αis a mediator of the intestinal inflammatory processes,thus being one of the main cytokines involved in the pathogenesis of IBD,however,its levels,when measured,are present in the serum of patients with *** addition,TNF-αplays an important role in promoting inflammation,such as the production of interleukins(IL),for instance IL-1βand *** are two receptors for TNF as following:The tumor necrosis factor 1 receptor(TNFR1);and the tumor necrosis factor 2 receptor(TNFR2).They are involved in the pathogenesis of IBD and their receptors have been detected in IBD and their expression is correlated with disease *** soluble TNF form binds to the TNFR1 receptor with,and its activation results in a signaling cascade effects such as apoptosis,cell proliferation and cytokine *** contrast,the transmembrane TNF form can bind both to TNFR1 and *** studies have suggested that TNF-αis one of the main pro-inflammatory cytokines involved in the pathogenesis of IBD,since TNF levels are present in the serum of both patients with UC and *** and subcutaneous biologics targeting TNF-αhave revolutionized the treatment of IBD,thus becoming the best available agents to induce and maintain IBD *** application of antibodies aimed at neutralizing TNF-αin patients with IBD that induce a satisfactory clinical response in up to 60%of patients,and also induced long-term maintenance of disease remission in most *** has been suggested that anti-TNF-αagents inactivate the pro-inflammatory cytokine TNF-αby direct neutralization,i.e.,resulting in suppression of ***,anti-TNF-αantibodies perform more complex functions than a simple block

关键词： Tumor necrosis factor 1 receptor Tumor necrosis factor 2 receptor Inflammatory bowel diseases Enteric nervous system tumor necrosis factor-alpha

Direct Electrochemical Determination of Glyphosate Herbicide Using a Screen-Printed Carbon Electrode Modified with Carbon Black and Niobium Nanoparticles

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Journal of Analysis and Testing 2023年第4期7卷 425-434页

作者： Julia Oliveira Fernandes Cassiano Augusto Rolim Bernardino Juliana dos Santos Fernandes Claudio Fernando Mahler Bernardo ferreira Braz Luiz henrique Carpenter dos Santos Rodrigo JoséCorrêa Ricardo Erthal Santelli Braulio Soares Archanjo Emerson Schwingel Ribeiro Fernando henrique Cincotto Departament of Analytical Chemistry Institute of ChemistryFederal University of Rio de JaneiroRio de Janeiro 21941-909Brazil Departament of Civil Engineering COPPEFederal University of Rio de JaneiroRio de Janeiro 21941-598Brazil National Institute of Science&Technology of Bioanalytics(INCTBio) CampinasSão Paulo 13083-862Brazil Departament of Inorganic Chemistry Institute of ChemistryFederal University of Rio de JaneiroRio de Janeiro 21941-909Brazil Unesp National Institute of Alternative Technologies for DetectionToxicological Assessment and Removal of Micropollutants and Radioactives(INCT-DATREM)AraraquaraSão Paulo 14800-060Brazil National Institute of Metrology Quality and TechnologyInmetroAv.Nossa Senhora das Graças50XerémDuque de Caxias 25250-020Brazil

Glyphosate is a herbicide that has been widely used worldwide and is used in agricultural areas to control weeds and unwanted *** sensors developed from different nanomaterials have high efficiency,excellent cost-benefit,and fast analysis time for detecting traces of environmental *** study aimed to produce an electrochemical sensor with disposable screen-printed electrodes based on carbon black modified with niobium nanoparticles to determine glyphosate in aqueous *** morphology,structure and electrochemical performance of the sensor were characterized by scanning electron microscopy,energy-dispersive X-ray spectroscopy,X-ray diffraction and cyclic *** pulse voltammetry in BR buffer solution at pH 5.0 allowed the generation of a method to quantify glyphosate concentration in a linear range of 5.90-172.30μmol/L(1.00-29.13μg/mL),with a limit of detection calculated at 3.07μmol/L(0.52μg/mL).The method efficiently quantified glyphosate in real water samples and showed no interference from K^(+),Na^(+),Ca^(2+),Mg^(2+)ions or thiamethoxam,imidacloprid and carbendazim pesticides.

关键词： Glyphosate Electrochemical sensor Carbon black Niobium nanoparticles

Ecophysiology and multivariate analysis for production of Tachigali vulgaris in Brazil:Influence of rainfall seasonality and fertilization

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林业研究:英文版 2023年第5期34卷 1289-1305页

作者： Pedro henrique Oliveira Simoes Candido ferreira de Oliveira Neto Manoel Tavares de Paula Dênmora Gomes de Araújo Rodrigo Silva do Vale Joao Olegário Pereira de Carvalho Universidade Federal de Mato Grosso Campus Universitario de SinopSinop 78550728Mato GrossoBrazil Universidade Federal Rural da Amazonia Campus BelemBelem 66077830ParaBrazil Universidade do Estado do Para Centro de Ciencias Naturais e TecnologiaBelem 66095015ParaBrazil Universidade Federal Rural da Amazonia Campus Capitao-PocoCapitao Poco 68650000ParaBrazil

Studies on fertilization management of species native to the Amazon for energy plantations contribute to the diversity of species use and reduce biological risk due to the excessive use of clones or hybrids of *** study evaluates the effect of precipitation seasonality and phosphorus and potassium fertilization on gas exchange in a Tachigali vulgaris *** levels of P(zero,65.2,130.4 kg ha^(-1))and three of K(zero,100.0,200.0 kg ha^(-1))were applied in a 3×3 factorial randomized block *** exchange measurements were conducted in April and November *** low rainfall,high irradiance period,photo synthetic rates were up to four times higher than in the high rainfall period,reaching 20.3μmol m^(-2)s^(-1)in the treatment with 130.4 g kg^(-1)of P and 100.0 g kg^(-1)of *** analysis and principal component analysis reduced the initial eight gas exchange variables to two and three principal components in periods of high and low rainfall,*** multivariate method used in this study readily identified variations in the variables as a function of rainfall,with high reliability in explaining the data set.

关键词： Photosynthesis rate Stomatal conductance Principal component analysis Factor analysis Tachigali vulgaris

Islet amyloid polypeptide & amyloid beta peptide roles in Alzheimer’s disease:two triggers, one disease

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Neural Regeneration Research 2021年第6期16卷 1127-1130页

作者： Sofia ferreira Ana F.raimundo Regina Menezes Ivo C.Martins iBET-Instituto de Biologia Experimental e Tecnológica OeirasPortugal CEDOC-Chronic Diseases Research Center Faculdade de Ciências MédicasUniversidade Nova de LisboaLisboaPortugal ITQB-NOVA Instituto de Tecnologia Química e Biológica António XavierUniversidade Nova de LisboaOeirasPortugal Instituto de Medicina Molecular Faculdade de MedicinaUniversidade de LisboaLisbonPortugal

Alzheimer’s disease(AD)is a neurodegenerative disorder that affects millions *** to population ageing,the incidence of AD is *** patients develop cognitive decline and dementia,features for which is known,requiring permanent *** poses a major socio-economic burden on healthcare systems as AD patients’relatives and healthcare workers are forced to cope with rising numbers of affected *** recent advances,AD pathological mechanisms are not fully ***,it is clear that the amyloid beta(Aβ)peptide,which forms amyloid plaques in AD patients’brains,plays a key *** 2 diabetes,the most common form of diabetes,affects hundreds of million people *** amyloid polypeptide(IAPP)is a hormone coproduced and secreted with insulin in pancreatic β-cells,with a key role in diabetes,as it helps regulate glucose levels and control adiposity and *** to Aβ,IAPP is very amyloidogenic,generating intracellular amyloid deposits that causeβ-cell dysfunction and *** is now clear that IAPP can also have a pathological role in AD,decreasing cognitive *** harms the blood-brain barrier,directly interacts and co-deposits with Aβ,promoting diabetes-associated *** can cause a metabolic dysfunction in the brain,leading to other diabetes-related forms of ***,here we discuss IAPP association with diabetes,Aβand dementia,in the context of what we designate a“diabetes brain phenotype”AD *** approach helps to set a conceptual framework for future IAPP-based drugs against AD.

关键词： aggregation Alzheimer amylin amyloid diabetes islet amyloid polypeptide

Peroxisome proliferator-activated receptor-alpha activation and dipeptidyl peptidase-4 inhibition target dysbiosis to treat fatty liver in obese mice

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World Journal of Gastroenterology 2022年第17期28卷 1814-1829页

作者： Flavia Maria Silva-Veiga Carolline Santos Miranda Isabela Macedo Lopes Vasques-Monteiro henrique Souza-Tavares Fabiane ferreira Martins Julio Beltrame Daleprane Vanessa Souza-Mello Department of Anatomy Institute of BiologyState University of Rio de JaneiroLaboratory of MorphometryMetabolism and Cardiovascular DiseasesRio de Janeiro 20551-030Brazil Department of Clinical and Toxicology Analysis State University of Rio de JaneiroNutrition InstituteState University of Rio de JaneiroNutrition InstituteUniversity of Sao PauloRio de Janeiro 20551-030Brazil

BACKGROUND Obesity and comorbidities onset encompass gut dysbiosis,altered intestinal permeability,and *** that target gut dysbiosis can cope with obesity and nonalcoholic fatty liver disease(NAFLD)*** proliferator-activated receptor(PPAR)-alpha activation and dipeptidyl-peptidase-4(DPP-4)inhibition alleviate NAFLD,but the mechanism may involve gut microbiota modulation and merits further *** To address the effects of PPAR-alpha activation and DPP-4 inhibition(isolated or combined)upon the gut-liver axis,emphasizing inflammatory pathways in NAFLD management in high-fat-fed C57BL/6J *** Male C57BL/6J mice were fed a control diet(C,10%of energy as lipids)or a highfat diet(HFD,50%of energy as lipids)for 12 wk,when treatments started,forming the groups:C,HF,HFA(HFD+PPAR-alpha agonist WY14643,2.5 mg/kg body mass),HFL(HFD+DPP-4 inhibitor linagliptin,15 mg/kg body mass),and HFC(HFD+the combination of WY14643 and linagliptin).RESULTS The HFD was obesogenic compared to the C *** treatments elicited significant body mass loss,and the HFC group showed similar body mass to the C *** treatments tackled oral glucose intolerance and raised plasma glucagon-like peptide-1 *** metabolic benefits restored Bacteroidetes/Firmicutes ratio,resulting in increased goblet cells per area of the large intestine and reduced lipopolysaccharides concentrations in treated *** the gene level,treated groups showed higher intestinal Mucin 2,Occludin,and Zo-1 expression than the HFD *** reduced endotoxemia suppressed inflammasome and macrophage gene expression in the liver of treated *** observations complied with the mitigation of liver steatosis and reduced hepatic triacylglycerol,reassuring the role of the proposed treatments on NAFLD *** PPAR alpha activation and DPP-4 inhibition(isolated or combined)tackled NAFLD in dietinduced obese mice by restoration of gut-liver axis

关键词： Nonalcoholic fatty liver disease High-fat diet Peroxisome proliferator-activated receptor-alpha Dipeptidyl-peptidase-4-inhibitor Dysbiosis Inflammation

Study of the roles of caspase-3 and nuclear factor kappa B in myenteric neurons in a P2X7 receptor knockout mouse model of ulcerative colitis

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World Journal of Gastroenterology 2023年第22期29卷 3440-3468页

作者： henrique Inhauser Riceti Magalhães Felipe Alexandre Machado Roberta Figueiroa Souza Marcos Antônio ferreira Caetano Vanessa Ribeiro Figliuolo Robson Coutinho-Silva Patricia Castelucci Department of Surgery School of Veterinary Medicine and Animal SciencesUniversity of São PauloSão Paulo 05508-270Brazil Department of Anatomy University of São PauloSão Paulo 05508-000Brazil Biophysics Institute Carlos Chagas Filho Federal University of Rio de JaneiroRio de Janeiro 21941-902Brazil

BACKGROUND The literature indicates that the enteric nervous system is affected in inflammatory bowel diseases(IBDs)and that the P2X7 receptor triggers neuronal ***,the mechanism by which enteric neurons are lost in IBDs is *** To study the role of the caspase-3 and nuclear factor kappa B(NF-κB)pathways in myenteric neurons in a P2X7 receptor knockout(KO)mouse model of *** Forty male wild-type(WT)C57BL/6 and P2X7 receptor KO mice were euthanized 24 h or 4 d after colitis induction by 2,4,6-trinitrobenzene sulfonic acid(colitis group).Mice in the sham groups were injected with *** mice were divided into eight groups(n=5):The WT sham 24 h and 4 d groups,the WT colitis 24 h and 4 d groups,the KO sham 24 h and 4 d groups,and the KO colitis 24 h and 4 d *** disease activity index(DAI)was analyzed,the distal colon was collected for immunohistochemistry analyses,and immunofluorescence was performed to identify neurons immunoreactive(ir)for calretinin,P2X7 receptor,cleaved caspase-3,total caspase-3,phospho-NF-κB,and total NF-κ*** analyzed the number of calretinin-ir and P2X7 receptor-ir neurons per ganglion,the neuronal profile area(μm^(2)),and corrected total cell fluorescence(CTCF).RESULTS Cells double labeled for calretinin and P2X7 receptor,cleaved caspase-3,total caspase-3,phospho-NF-κB,or total NF-κB were observed in the WT colitis 24 h and 4 d *** number of calretinin-ir neurons per ganglion was decreased in the WT colitis 24 h and 4 d groups compared to the WT sham 24 h and 4 d groups,respectively(2.10±0.13 vs 3.33±0.17,P<0.001;2.92±0.12 vs 3.70±0.11,P<0.05),but was not significantly different between the KO *** calretinin-ir neuronal profile area was increased in the WT colitis 24 h group compared to the WT sham 24 h group(312.60±7.85 vs 278.41±6.65,P<0.05),and the nuclear profile area was decreased in the WT colitis 4 d group compared to the WT sham 4 d group(104.63±2.49 vs 117.41±1.14,P<0.01).The number of

关键词： Cell death Enteric nervous system Gastroenterology Inflammatory bowel diseases P2X7 receptor Purinergic signaling

Communicating with the non-dominant hemisphere:Implications for neurological rehabilitation

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Neural Regeneration Research 2013年第13期8卷 1236-1246页

作者： Fabricio ferreira de Oliveira Sheilla de Medeiros Correia Marin Paulo henrique ferreira Bertolucci Department of Neurology and Neurosurgery Escola Paulista de Medicina Federal University of So Paulo (UNIFESP) So Paulo SP

Aphasic syndromes usually result from injuries to the dominant hemisphere of the brain. Despite the fact that localization of language functions shows little interindividual variability, several brain areas are simultaneously activated when language tasks are undertaken. Mechanisms of language recovery after brain injury to the dominant hemisphere seem to be relatively stereotyped, including activations of perilesional areas in the acute phase and of homologues of language areas in the non-dominant hemisphere in the subacute phase, later returning to dominant hemisphere activation in the chronic phase. Plasticity mechanisms reopen the critical period of language development, more specifically in what leads to disinhibition of the non-dominant hemisphere when brain lesions affect the dominant hemisphere. The non-dominant hemisphere plays an important role during recovery from aphasia, but currently available rehabilitation therapies have shown limited results for efficient language improvement. Large-scale randomized controlled trials that evaluate well-defined interventions in patients with aphasia are needed for stimulation of neuroplasticity mechanisms that enhance the role of the non-dominant hemisphere for language recovery. Ineffective treatment approaches should be replaced by more promising ones and the latter should be evaluated for proper application. The data generated by such studies could substantiate evidence-based rehabilitation strategies for patients with aphasia.

关键词： neural regeneration reviews linguistics aphasia language speech non-dominant hemisphere disability evaluation prognosis cerebrum function grants-supported paper neuroregeneration