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Assessment of spermatogenesis in camels via seminiferous tubule staging and testosterone profiling

Asian pacific Journal of Reproduction 2024年第3期13卷 133-142页

作者： Saif Ullah Abdul Quddus Quanwei Wei Wael Ennab Sheeraz Mustafa Jiahao Sun rahmani.mohammad malyar Abdul Baseer Dagan Mao Fangxiong Shi College of Animal Science and Technology Nanjing Agricultural UniversityNanjing 210095China Faculty of Veterinary and Animal Sciences Lasbela University of Agriculture Water and Marine SciencesBalochistanPakistan Faculty of Veterinary and Animal Sciences Ziaddin University Karachi Pakistan

Objective:To investigate the successive morphological stages of spermatogenesis,hormonal regulation,and testosterone profile in dromedary camel ***:Testicular tissue samples were obtained from 12 dromedary bull camels aged 7 to 8 at a local *** histological assessment involved tissue processing,hematoxylin and eosin(H&E)staining,and examination under a *** analysis,germ cell identification,and assessment of seminiferous tubules and maturation were *** assay was performed by radioimmunoassay using blood samples collected at regular ***:The study revealed 12 phases of the dromedary camel's seminiferous epithelium cycle,highlighting distinct morphological characteristics and cellular *** migration,maturation,cap formation,and the Golgi-mediated synthesis of proacrosomal vesicles were also explained in dimension,as were the steps of acrosome *** and mature sperm cells were present when spermatogenesis phases were *** analysis of the dimensions of seminiferous tubules revealed specific measures for diameter,area,and epithelial height about luminal ***,there were noticeable variations in the serum testosterone concentrations during the study period,indicating temporal ***:This study outlines the spermatogenesis process in dromedary camels across 12 stages,emphasizing cellular dynamics and acrosomal *** also provides seminiferous tubule measurements and observes seasonal testosterone fluctuations,offering insights into reproductive regulation and potential strategies for camel breeding conservation.

关键词： Spermatogenesis Camel Testosterone profile Morphological staging

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富硒/锌益生菌对热应激大鼠生长性能、血清酶活性、抗氧化能力、炎症因子、肝脏状态及相关基因表达的影响

富硒/锌益生菌对热应激大鼠生长性能、血清酶活性、抗氧化能力、...

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作者： rahmani.mohammad malyar 南京农业大学

学位级别：硕士

气候对家畜的性能、健康和福利有很大的影响。较高的环境温度和湿度是造成动物压力的环境压力源。热应激是降低家畜生产、繁殖和免疫力的主要因素。硒、锌等微量元素是机体维持健康和免疫力的必需元素,在抗氧化防御和免疫功能中发挥着... 详细信息

气候对家畜的性能、健康和福利有很大的影响。较高的环境温度和湿度是造成动物压力的环境压力源。热应激是降低家畜生产、繁殖和免疫力的主要因素。硒、锌等微量元素是机体维持健康和免疫力的必需元素,在抗氧化防御和免疫功能中发挥着重要作用。它们参与了生长、生产和繁殖,并且是对动物免疫重酶的辅因子。最近,益生菌被定义为活微生物。应用足够数量益生菌能对宿主健康产生有益的影响。因此,本研究的目的是评估富硒/锌益生菌对在高温环境下饲养的Wistar大鼠的生长性能、抗氧化状态、血清酶活性、免疫功能、相关基因表达、肝脏生理功能和形态学特征的影响。试验一:富锌益生菌改善热应激大鼠的生长性能、抗氧化状态、免疫功能、相关基因表达及形态学特征本试验研究了富锌益生菌(ZnP)对夏季高温应激Wistar大鼠生长性能、抗氧化状态、免疫功能、相关基因表达及形态学特征的影响。将36只6周龄雄性Wistar大鼠随机分为3组(12只/组):饲喂基础日粮(对照组)、基础日粮+益生菌(P)和基础日粮+富锌益生菌(ZnP,100mg/L),连续饲养40天。实验最后一天采用心内法采血,并采集肝、心、肾组织。结果表明,P和ZnP均能显著提高大鼠生长性能(P<0.05)。结果显示,ZnP显著增加大鼠血清中谷胱甘肽含量、提高谷胱甘肽过氧化物酶和超氧化物歧化酶活性,但降低血清丙二醛水平。与对照组相比,P和ZnP处理组大鼠的IL-2、IL-6、IFN-γ含量显著升高(P<0.05),IL-10含量显著降低(P<0.05)。此外,ZnP对SOD1、SOD2、MT1和MT2基因的表达有显著的上调作用(P<0.05),而对Hsp90和Hsp70热休克基因的表达分别有显著的下调作用(P<0.05)。苏木精和伊红染色结果显示,P和ZnP均能引起大鼠绒毛高度和肠壁厚度的变化。综上所述,富锌益生菌可通过增强大鼠抗氧化状态、免疫功能、相关基因表达和肠道形态特征,提高其在高温环境下的生长性能。因此,本品可作为高温应激条件下Wistar大鼠的潜在营养补充剂。试验二:富硒/锌益生菌对热应激大鼠血清酶活性、抗氧化能力、炎症因子及相关基因表达的影响本试验旨在研究富硒/锌益生菌(SeZnP)对环境高热应激大鼠生长性能、血清酶活性、抗氧化能力、炎症因子及基因表达的影响。将60只6周龄雄性Wistar大鼠随机分为5组(12只/组),分别饲喂基础日粮(对照组)、基础日粮+益生菌(P)、基础日粮+富锌益生菌(ZnP,100mg/L)、基础日粮+富硒益生菌(SeP,0.3mg/L)、基础日粮+富硒/锌益生菌(SeZnP,0.3mg+100mg/L),连续饲养30天。采用商品化试剂盒检测血液和组织中的血清酶活性、抗氧化能力和炎症因子;采用qRT-PCR检测抗氧化物质、热休克和炎症相关分子的表达。结果显示,与P、ZnP、SeP或SeZnP组相比,热应激显著提高了天冬氨酸转氨酶、丙氨酸转氨酶、乳酸脱氢酶、肌酸激酶、血尿素氮、肌酐和碱性磷酸酶水平(P<0.01)。而补充ZnP、SeP和SeZnP可显著提高谷胱甘肽含量、谷胱甘肽过氧化物酶和超氧化物歧化酶活性,降低丙二醛含量(P<0.05)。IL-2、IL-6、IL-8浓度显著升高,IL-10浓度显著降低(P<0.05)。GPx1和SOD1基因表达明显增加,COX-2、iNOS、HSP70和HSP90 mRNA表达水平明显降低(P<0.05)。最后,SeZnP对上述参数的影响最大。本试验结果表明,对于在高环境温度下饲养的Wistar大鼠,补充SeZnP可能比单独补充ZnP或SeP具有更高的营养价值。试验三:富硒益生菌对热应激大鼠的抗炎和抗氧化保护作用本研究旨在探讨富硒益生菌对环境高热应激大鼠Wistar大鼠肝脏的影响。将48只10周龄雄性Wistar大鼠随机分为4组(12只/组),分别饲喂基础日粮(对照组)、高温+基础日粮组(HT)、高温+益生菌组(HT+P,基础日粮中添加嗜酸乳杆菌1011/mL和酿酒酵母109/mL),高温+富硒益生菌组(HT+SeP,基础日粮中含硒0.3mg/kg,嗜酸乳杆菌1011/mL和酿酒酵母109/mL)。分别饲喂42天后,HT组、HT+P组和HT+SeP组暴露于较高的环境温度(40-42℃)下饲养,对照组在室温(25℃)下饲养。在高温环境下暴露42天后,采集血液和肝组织。结果表明,HT组大鼠体重显著下降,肝重和肝指数下注升高(P<0.05)。组织学检查显示肝窦扩张,HT组肝间质静脉淤血。与对照组相比,HT组肝组织丙二醛含量(P<0.01)、血清AST、ALT、ALP、LDH含量显著升高(P<0.05),而肝组织SOD、T-AOC、GSH-PX活性和血清总蛋白含量显著降低(P<0.05)。HT+P组和HT+SeP组肝组织形态学显示损伤明显恢复,血清门冬氨酸氨基

关键词：富硒/锌益生菌血清酶活性抗氧化能力炎症因子相关基因表达 Wistar 大鼠

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An Experience of Short-Term Results of Laparoscopic Inguinal Hernioplasty Using 3D Mesh in a Developing Country

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International Journal of Clinical Medicine 2015年第1期6卷 64-69页

作者： Iqbal Saleem Mir Alfer Ah Nafae Aijaz Ahmed malyar Muntakhab Nafae Yawar Watali Mudasir Farooq Shahnawaz Bashir Bhat Sheikh Viqar The Post-Graduate Department of Surgery Govt Medical College Srinagar India

Background: The experience of short term results of laparoscopic inguinal hernia repair using 3D mesh in a developing country is reviewed. Methods: From January 2012 to February 2014, 53 patients underwent laparoscopic inguinal hernioplasty. A retrospective case series of 53 consecutive patients undergoing TEP/TAPP by a single surgical team was followed prospectively with a focused physical examination and interview. 4 out of 53 patients had recurrent hernia following open repairs and 49 had primary hernias. Data collected included operative time, intraoperative bleeding, intraoperative difficulties, immediate postoperative pain, chronic groin pain, recurrence, sensory disturbance, activity or occupational limitation and personal satisfaction. Results: All the patients were male aged 32 to 75 years with a mean age of 53.5 years. Mean operative time was 37.4 minutes;intraoperative dissection, blood loss were less;and immediate postoperative pain was negligible as assessed by VAS. There was no mortality or major morbidity. Mean follow-up was 12 months (2 to 18 months). Follow-up was completed by interview and physical examination. Hernia was not found to recur during the follow up period. Chronic pain occurred in 2 patients (3.7%), which was mild in nature. Ninety-seven percent of patients were satisfied with their repair and would or had recommended TEP/TAPP to others using 3D Mesh. Conclusions: Short-term results of TEP/TAPP hernia repair using 3D mesh demonstrated to be an effective and safe procedure with low prevalence of chronic pain that is generally of a mild, infrequent nature. It was also concurred that there is decrease in operative time. Manipulation of mesh was significantly reduced. Intraoperative bleeding and use of post operative analgesia was reduced considerably. There was no recurrence, however the cost of the mesh increased the overall cost of the procedure acting as a limiting factor in a developing country.

关键词： Inguinal Hernia 3D Prolene Mesh Totally Extra-Peritoneally (TEP) Trans-Abdominal Pre-Peritoneal (TAPP)

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