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Multiplex real-time PCR revealed very high prevalence of soil-transmitted helminth infections among aborigines in Peninsular Malaysia

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Asian Pacific Journal of Tropical Medicine 2020年第12期13卷 550-556页

作者： nurulhasanah Othman noorizan Miswan Weng-kin Wong Boon-huat Lim rahmah n.ordin Institute for Research in Molecular Medicine Universiti Sains Malaysia11800 PenangMalaysia School of Health Sciences Universiti Sains Malaysia16150 KelantanMalaysia

Objective:To determine the true prevalence of soil-transmitted helminth infections in the Malaysian aborigines using real-time ***:A total of 122 aborigines from seven tribes were recruited from settlements and nearby hospitals which served the communities,located in four states in Peninsular *** stool samples were examined for the presence of soil-transmitted helminth using real-time PCR and *** latter included the direct wet mount and formalin-ether concentration technique(FECT).The infection load in FECT-positive samples was determined by the Kato-Katz *** real-time analyzer detected five helminth species using two sets of ***:The real-time PCR detected soil-transmitted helminth in 98.4% samples(n=122),which were 1.56 times higher than by *** lumbricoides and Trichuris trichiura were detected in more than 90% of the samples,while hookworm was detected in 46.7%(necator americanus) and 13.9%(Ancylostoma sp.)of the *** with previous reports on the Malaysian aborigines showed that the real-time PCR markedly improved the detection of Ascaris lumbricoides,hookworm and Strongyloides *** real-time PCR detected poly-helminths in 92.6%of the samples compared to 28.7% by *** addition,27 samples(22.1%) showed amplification of Strongyloides stercoralis ***:The real-time PCR showed very high prevalence rates of soil-transmitted helminth infections in the aborigines and is the recommended method for epidemiological investigation of soiltransmitted helminth infections in this population.

关键词： prevalence infections markedly

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A phase inversion polymer coating to prevent swelling and spalling of clay fines in coal seam gas wells

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International Journal of Coal Science & Technology 2018年第2期5卷 179-190页

作者： Lei Ge Christopher Hamilton rahmah Tasha Febrina Victor Rudolph Thomas E. Rufford School of Chemical Engineering The University of Queensland Saint Lucia QLD 4072 Australia Centre for Future Materials University of Southern Queensland Springfield Central QLD 4300 Australia

We report a phase inversion polymer coating as a novel concept with potential to prevent clay swelling and fines generation in coal seam gas, or other petroleum, wellbores. Our approach uses polyethersulfone （PES） with n-methyl-2- pyrrolidone （nMP） as a water-soluble solvent to form a dense, low-porosity film across the clay-rich interburden layers, but a porous and permeable membrane on coal seams. This contrasting behaviour occurs because the coal contains much more free water than the clay-rich interburden layers. We demonstrate the efficacy of the method to prevent clay spalling in immersion tests and under a flow of fresh water in a visual swell test apparatus. The clay-rich rocks studied were mudstone and siltstone, and these were dip coated in the PES/nMP solution. The uncoated mudstone swelled and broke apart quickly in the immersion test and visual flow test, but the PES coated rock samples were stable for 30 days. The coated rock and coal samples were characterised by X-ray diffraction, Fourier transform infrared spectroscopy and scanning electron microscopy. The morphology of coated mudstone and coated coal samples showed that the polymer formed a dense layer across the low-permeability mudstone, but an open porous structure on the coal surface. The effect of the coating on the permeability of KCl brine through coal was measured in a core-flood apparatus. Although the permeability of the coal showed some deterioration after coating, from （0.58 ± 0.12） mD to （0.3 ±0.03） mD, these results demonstrate the potential of a smart polymer coating to prevent clay swelling while remaining permeable to gas and water on coal layers.

关键词： Coal bed methane Formation damage Smectite Wellbore stability

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Epidemiology and immunodiagnostics of Strongyloides stercoralis infections among migrant workers in Malaysia

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Asian Pacific Journal of Tropical Medicine 2019年第6期12卷 250-257页

作者： norhidayu Sahimin Yvonne A.L.Lim rahmah n.ordin Muhammad Hafiznur Yunus norsyahida Arifin Jerzy Marian Behnke Siti nursheena Mohd Zain Tropical Infectious Diseases Research and Education Centre(TIDREC) University of Malaya50603 Kuala LumpurMalaysia Department of Parasitology Faculty of MedicineUniversity of MalayaKuala Lumpur.Malaysia Institute for Research in Molecular Medicine(InFORMM) Universiti Sains Malaysia11800 Pulau PinangMalaysia School of Life Sciences University of NottinghamUniversity ParkNottinghamNG7 2RDUK Institute of Biological Science Faculty of ScienceUniversity of MalayaKuala LumpurMalaysia

Objective: To investigate the status of Strongyloides(S.) stercoralis infections among migrant workers in Malaysia for the first time and identify risk ***: Four diagnostic methods were employed for the detection of S. stercoralis including microscopy, enzyme-linked immunosorbent assay(ELISA) using a commercial kit, ELISA using the rSs1a antigen and polymerase chain reaction(PCR). Low and semi-skilled workers from five working sectors(i.e. manufacturing, food service, agriculture and plantation, construction and domestic service) were tested on a voluntary basis. Results: The overall seroprevalence of S. stercoralis from 483 workers employing the ELISA commercial kit for IgG was 35.8%(n=173;95% CI: 31.5%-40.1%) whereas seroprevalence using the rSs1a-ELISA was 13.0%(n=63;95% CI: 10.0%-16.0%). Cross tabulation between the ELISA commercial kit and rSs1a-ELISA showed that only 6.4%(n=31;95% CI: 4.2%-8.6%) of the samples were positive in both tests. Microscopic examination of all 388 fecal samples were negative;however subsequent testing by a nested PCR against DnA from the same samples successfully amplified DnA from three male subjects(0.8%;3/388). Male workers, India and Myanmar nationality, food service occupation and those living in the hostel were statistically significant with seroprevalence(Pnclusion: This is the first report on the epidemiology of S. stercoralis infections among the migrant workers in Malaysia. Our results highlight the importance of using appropriate diagnostic tools for detection. The presence of anti-S. stercoralis antibodies in the study population calls for improvements in personal hygiene and sanitation standards among migrant workers in Malaysia through control strategies including health education campaigns and programs aimed at increasing awareness and healthy behaviors.

关键词： Strongyloides stercoralis Migrant workers Microscopic examination Serological analysis PCR

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Separation of Inorganic Anions Using Methacrylate-Based Monolithic Column Modified with Trimethylamine in Ion Chromatography Capillary System

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American Journal of Analytical Chemistry 2013年第9期4卷 451-456页

作者： Fitri Mairizki Athika rahmah &nbsp Hilma Radhia Putri Rahmiana Zein Lee Wah Lim Toyohide Takeuchi Edison Munaf Department of Chemistry Faculty of Engineering Gifu University Gifu Japan Department of Chemistry Faculty of Mathematics and Natural Sciences Andalas Universiy Padang Indonesia

Methacrylate-based monolithic column was prepared in fused-silica capillary (80 ′ 0.32 mm i.d.) by in situ polymerizetion reaction using glycidyl methacrylate as monomer;ethylene dimethacrylate as crosslinker;1-propanol, 1,4-butanediol, and water as porogenic solvents. The monolith matrix was modified with trimethylamine to create strong anion exchanger via ring opening reaction of epoxy groups. The morphology of the monolithic column was studied by using Scanning Electron Microscope (SEM). This column had good mechanical stability and permeability. The effects of various mobile phases for separation of inorganic anions were investigated. Iodate, bromate, nitrite, bromide, and nitrate were separated within 11 min using100 mMpotassium chloride as mobile phase and detected at 210 nm. This method showed good precision of retention time, acceptable linearity and good sensitivity. Under the optimum condition, the RSD of the retention time was in the range of 1.09%-1.75% (n = 6). The calibration curve showed linear relationships between the peak area and the concentration. The limits of detection (LOD) and the limits of quantitation (LOQ) were between 0.08-0.18 mM and 0.26-0.61 mM, respectively. This method was applied to the determination of inorganic anions in tap water and ground water samples.

关键词： Methacrylate-Based Monolithic Column Trimethylamine Ion Chromatography Capillary System Inorganic Anions Water Sample

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Entamoeba histolytica acetyl-CoA synthetase:biomarker of acute amoebic liver abscess

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Asian Pacific Journal of Tropical Biomedicine 2014年第6期4卷 446-450页

作者： Lim Boon Huat Alfonso Olivos Garcia Tan Zi ning Wong Weng Kin rahmah n.ordin Siti shafiqah Anaqi Azham Lee zhi Jie Guee Cher Ching Foo Phiaw Chong Pim Chau Dam Biomedicine Programme.School of Health Sciences Universiti Sains Malaysia Departmento de Medicina Experimental Facultad de MedicinaUniversidad Nacional Autonoma de Mexico Department of Medicine Allianze University College of Medical Sciences Institute for Research in Molecular Medicine 11800 University Sains Malaysia

Objective:To characterize the Entamoeba histolytica(***)antigen(s)recognized by moribound amoebic liver abscess ***:Crude soluble antigen of *** was probed with sera of moribund hamsters in 1D-and 2D-Westem blot *** antigenic protein was then sent for tandem mass spectrometry *** corresponding gene was cloned and expressed in Escherichia coli BL21-AI to produce the recombinant *** ADP-forming acetyl-CoA synthetase(EhACS)protein.A customised ELISA was developed to evaluate the sensitivity and specificity of the recombinant ***:A^75 kDa protein band with a pl value of 5.91-6.5 was found to be antigenic;and not detected by sera of hamsters in the control *** mass spectrometry analysis revealed the protein to be the 77 kDa *** ADP-forming acetyl-CoA synthetase(EhACS).The customised ELISA results revealed 100%sensitivity and 100%specificity when tested against infected(n=31)and control group hamsters(n=5)serum samples,***:This rinding suggested the significant role of EhACS as a biomarker for moribund hamsters with acute amoebic liver abscess(ALA)*** is deemed pertinent that future studies explore the potential roles of EhACS in better understanding the pathogenesis of ALA;and in the development of vaccine and diagnostic tests to control ALA in human populations.

关键词： Entamoeba histolytica Amoebic liver abscess Biomarker Acetyl-CoA synthetase Recombinant EhACS Western blot

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