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Chemical composition of essential oils and in vitro antioxidant activity of fresh and dry leaves crude extracts of medicinal plant of Lactuca Sativa *** to sultanate of Oman

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Asian Pacific Journal of Tropical Biomedicine 2013年第5期3卷 353-357页

作者： rahma said salim al nomaani Mohammad Amzad Hossain Afaf Mohammed Weli Qasim al-Riyami Jamal Nasser al-Sabahi School of Pharmacy College of Pharmacy and NursingUniversity of Nizwa Central Instrument Laboratory College of Agriculture and Marine SciencesSultan Qaboos University

Objective:To isolate and analyse the chemical composition in the essential oils and free radical scavenging activity of different crude extracts from the fresh and dry leaves of vegetable plants of Lactuca sativa L.(***).Methods:The essential oils and volatile chemical constituents were isolated from the fresh and dry leaves of ***(lettuce)grown in Sultanate of Oman by hydro distillation *** antioxidant activity of the crude extracts was carried out by well established free radical scavenging activity(DPPH)***:About 20 chemical compounds of different concentration representing 83.07%and 79.88%respectively were isolated and identified by gas chromatography-mass spectroscopy in the essential oils isolated from the fresh and dry leaves asα-pinene(5.11%and 4.05%).γ-cymene(2.07%and 1.92%),thymol(11.55%and 10.73%),durenol(52.00%and 49.79%),α-terpinene(1.66%and 1.34%),thymol acetate(0.99%and 0.67%),caryophyllene(2.11%and 1.98%),spathulenol(3.09%and 2.98%).camphene(4.11%and3.65%),limonene(1.28%and 1.11%)representing these major chemical ***,some other minor chemical constituents were also isolated and identified from the essential oil of lettuce including p-pinene,α-terpinolene,linalool,4-terpineol,α-terpineol,o-methylthymol,L-alloaromadendrene and ***:The chemical consliluents in the essential oils from the locally grown lettuce were identified in the following classes or groups of chemical compounds such as monoterpenes,sesquiterpenes volatile organic compounds and their oxygenated ***,the essential oils and the crude extracts from Omani vegetable species of lettuce are active candidates which would be used as antioxidant,antifungal or antimicrobial agents in new drugs preparation for therapy of infectious diseases.

关键词： Lactuca saliva Omani lettuce Clevenger apparatus Essential oil Durenol Thymol Soxhlet extractor Organic crude extracts Antioxidant activity GC-MS analyses

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