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Detection of ROS and translocation of ERP-57 in apoptotic induced human neuroblastoma(SH-SY5Y)cells

BIOCELL 2019年第3期43卷 167-174页

作者： ATIF KAMIL MUBARAK ALI khan MUHAMMAD AASIM NADIR ZAMAN khan raham sher khan MUHSIN JAMAL WAQAR AHMAD MIR AZAM khan FAZAL JALIL Department of Biotechnology Abdul Wali Khan University Mardan(AWKUM)Garden CampusToru RoadMardanKPKPakistan Department of Biotechnology University of Malakand(UOM)ChakdaraLower DirPakistan Department of Microbiology Abdul Wali Khan University Mardan(AWKUM)Garden CampusToru RoadMardanKPKPakistan Department of Pharmacy University of Malakand(UOM)ChakdaraLower DirPakistan

Several toxic compounds are known to induce apoptosis in mammalian cell *** human neuroblastoma cells(SHSH-SY5Y)were exposed to the phosphatase inhibiting toxin okadaic acid(OA)or hydrogen peroxide(H2O2)to induce apoptosis as well as generate reactive oxygen species(ROS).Mitoxantrone(MXT)was used as a positive control for *** SHSH-SY5Y cells were transfected with eukaryotic expression plasmid pHyPer-dMito encoding mitochondrial-targeted fluorescent or pHyPer-dCito encoding cytoplasmic-targeted fluorescent sensor for hydrogen peroxide(HyPer).The ERp57,also called GRP58(Glucose-regulated protein 58),is a stress protein induced in conditions like glucose starvation and viral *** ERp57 was shown to translocate from the endoplasmatic reticulum to the cell surface in anthracycline-induced apoptotic ***57 co-translocation together with calreticulin has been suggested to be crucial for recognizing tumor cells to induce immunogenic cell ***57 translocation after exposure to okadaic acid was studied using immunofluorescence and confocal *** studies indicated that okadaic acid has induced the translocation of ERp57 to the cellular membrane.

关键词： Apoptosis Cytoplasm Endoplasmic recticulum(ER) ERP-57 Human neuroblastoma cell(SHSH-SY5Y) Immunofluorescence Mitochondria Reactive oxygen species(ROS) Transfection

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Nitrogen management improves lodging resistance and production in maize(Zea mays L.)at a high plant density

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Journal of Integrative Agriculture 2023年第2期22卷 417-433页

作者： Irshad AHMAD Maksat BATYRBEK Khushnuma IKRAM Shakeel AHMAD Muhammad KAMRAN Misbah raham sher khan HOU Fu-jiang HAN Qing-fang Key Laboratory of Crop Physio-Ecology and Tillage Science in North-Western Loess Plateau Ministry of Agriculture/College of AgronomyNorthwest A&F UniversityYangling 712100P.R.China State Key Laboratory of Herbage Improvement and Grassland Agro-Ecosystems/Key Laboratory of Grassland Livestock Industry Innovation Ministry of Agriculture and Rural Affairs/Engineering Research Center of Grassland IndustryMinistry of Education/College of Pastoral Agriculture Science and TechnologyLanzhou UniversityLanzhou 730020P.R.China Department of Biotechnology Abdul Wali Khan University MardanMardan 23200Pakistan

Lodging in maize leads to yield losses *** this study,we determined the effects of traditional and optimized nitrogen management strategies on culm morphological characteristics,culm mechanical strength,lignin content,root growth,lodging percentage and production in maize at a high plant *** compared a traditional nitrogen(N)application rate of 300 kg ha–1(R)and an optimized N application rate of 225 kg ha^(–1)(O)under four N application modes:50%of N applied at sowing and 50%at the 10th-leaf stage(N1);100%of N applied at sowing(N2);40%of N applied at sowing,40%at the 10th-leaf stage and 20%at tasseling stage(N3);and 30%of N applied at sowing,30%at the 10th-leaf stage,20%at the tasseling stage,and 20%at the silking stage(N4).The optimized N rate(225 kg ha^(–1))significantly reduced internode lengths,plant height,ear height,center of gravity height and lodging *** optimized N rate significantly increased internode diameters,filling degrees,culm mechanical strength,root growth and lignin *** application of N in four split doses(N4)significantly improved culm morphological characteristics,culm mechanical strength,lignin content,and root growth,while it reduced internode lengths,plant height,ear height,center of gravity height and lodging *** diameters,filling degrees,culm mechanical strength,lignin content,number and diameter of brace roots,root volume,root dry weight,bleeding safe and grain yield were significantly negatively correlated with plant height,ear height,center of gravity height,internode lengths and lodging *** conclusion,treatment ON4 significantly reduced the lodging percentage by improving the culm morphological characteristics,culm mechanical strength,lignin content,and root growth,so it improved the production of the maize crop at a high plant density.

关键词： high plant density lodging resistance maize nitrogen rates nitrogen application modes

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RNAi-Mediated Resistance to Cucumber Mosaic Virus (CMV) in Genetically Engineered Tomato

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American Journal of Plant Sciences 2014年第5期5卷 554-572页

作者： Valentine Otang Ntui Kynet Kong Pejman Azadi raham sher khan Dong Poh Chin Tomoko Igawa Masahiro Mii Ikuo Nakamura Laboratory of Plant Cell Technology Graduate School of Horticulture Chiba University Chiba Japan

Cucumber mosaic virus is one of the most constraints to the production of tomato and other vegetable crops worldwide. Here, we generated an RNAi construct containing inverted repeat of 1138 bp fragment of a partial replicase gene of CMV-O and used it to produce transgenic tomato plants expressing CMV-specific dsRNA of the replicase gene. Inoculation of transgenic plants with CMV strain O discriminated three categories of plants: plants that showed complete resistance, which were free of symptoms;highly resistant plants, which had mild symptoms, but later recovered because new leaves that emerged were free of symptoms;and susceptible plants, which showed severe symptoms similar to wild-type plants. The completely resistant lines were selected and challenged with a closely related strain, CMV-Y. Interestingly, the transgenic plant lines either remained immune or showed high levels of resistance to the strain. No virus could be detected in uninoculated new leaves of the resistant lines after RT-PCR and Dot immunobinding assay (DIBA) analyses. We could show that the resistance is correlated with post-transcriptional gene silencing because of the production of transgenic specific siRNA.

关键词： CMV dsRNA PTGS Replicase Gene RNAi Tomato

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