检索结果-南通市图书馆

Expression and significant roles of the long non-coding RNA cASc19/miR-491-5p/HMGA2 axis in the development of gastric cancer

在线全文

学校读者我要写书评

暂无评论

World Journal of Gastrointestinal Oncology 2024年第8期16卷 3559-3584页

作者： Li-Xiang Zhang Pan-Quan Luo Zhi-Jian Wei A-man Xu Tao Guo Department of Gastroenterology The First Affiliated Hospital of Anhui Medical UniversityHefei 230031Anhui ProvinceChina Anhui Provincial Key Laboratory of Digestive Disease Hefei 230031Anhui ProvinceChina Department of General Surgery The First Affiliated Hospital of Anhui Medical UniversityHefei 230031Anhui ProvinceChina Anhui Public Health clinical center Hefei 230000Anhui ProvinceChina

BAcKGROUND Gastric cancer(Gc)is a common malignant tumor,long non-coding RNA and microRNA(miRNA)are important regulators that affect tumor proliferation,metastasis and chemotherapy resistance,and thus participate in tumor ***19 is a new bio-marker which can promote tumor invasion and ***,the mechanism by which cASc19 affects the progression of Gc through miRNA is not *** To explore the role of the cASc19/miR-491-5p/HMGA2 regulatory axis in *** To explore the expression and prognosis of cASc19 in Gc through clinical samples,and investigate the effects of inhibiting cASc19 on the proliferation,migration,invasion and other functions of Gc cells through cell counting Kit-8(ccK-8),ethynyldeoxyuridine,Wound healing assay,Transwell,Western blot and flow cytometry *** effect of miR-491-5p and HMGA2 in Gc were also *** regulatory relationship between cASc19 and miR-491-5p,miR-491-5p and HMGA2 were validated through Dual-luciferase reporter gene assay and reverse transcription *** ccK-8,Transwell,Wound healing assay,flow cytometry and animal experiments verify the role of cASc19/miR-491-5p/HMGA2 regulatory *** The expression level of cASc19 is related to the T stage,N stage,and tumor size of *** of the expression of cASc19 can inhibit the ability of proliferation,migration,invasion and EMT conversion of Gc cells,and knocking down the expression of cASc19 can promote the apoptosis of Gc *** the expression of miR-491-5p can inhibit the proliferation of Gc cells,miR-491-5p mimics can inhibit EMT conversion,and promote the apoptosis of Gc cells,while decreasing the expression of miR-491-5p can promote the proliferation and EMT conversion and inhibit the apoptosis of Gc *** expression of HMGA2 in Gc tissues is higher than that in adjacent *** the same time,the expression level of HMGA2 is related to the N and T stages of the *** the level of HMGA2 can pr

关键词： Gastric cancer Long non-coding RNA cASc19 miR-491-5p HMGA2 Prognosis

Immunoregulatory and Anti-cancer Activities of combination Treatment of Novel Four-Herb Formula and Doxorubicin in 4T1-Breast cancer Bearing Mice

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

chinese Journal of Integrative Medicine 2024年第4期30卷 311-321页

作者： Lea Ling-Yu Kan Ben chung-Lap chan Grace Gar-Lee Yue LI Pei-ting Sharon Sze-man Hon HUANG Dan-qi Miranda Sin-man Tsang clara Bik-San Lau Ping-chung Leung chun-Kwok Wong Institute of chinese Medicine The Chinese University of Hong KongHong Kong SARChina State Key Laboratory of Research on Bioactivities and clinical Applications of Medicinal Plants The Chinese University of Hong KongHong KongChina Department of chemical Pathology The Chinese University of Hong KongHong Kong SARChina china-Australia International Research centre for chinese Medicine School of Health and Biomedical SciencesSTEM CollegeRMIT UniversityBundooraVictoriaAustralia Li Dak Sum Yip Yio chin R&D centre for chinese Medicine The Chinese University of Hong Kong SARHong KongChina

Objective To investigate the in vivo immunomodulatory and anti-tumor mechanisms of the combined treatment of novel Four-Herb formula(4HF)and doxorubicin in triple-negative breast cancer(TNBc).Methods Murine-derived triple-negative mammary carcinoma cell line,4T1 cells,was cultured and inoculated into mouse mammary ***-six mice were randomly assigned into 6 groups(n=11 in ench):naive,control,LD 4HF(low dose 4HF),HD 4HF(high dose 4HF),LD 4HF+D(low dose and doxorubicin),and D(doxorubicin).Apart from the naive group,each mouse received subcutaneous inoculation with 5×10^(5)4T1 cells resuspended in 100µL of normal saline in the mammary fat *** from the day of tumor cell inoculation,tumors were grown for 6 *** LD and HD groups received daily oral gavage of 658 and 2,630 mg/kg 4HF,*** LD 4HF+D group received daily oral gavage of 658 mg/kg 4HF and weekly intraperitoneal injection of doxorubicin(5 mg/kg).The D group received weekly intraperitoneal injections of doxorubicin(5 mg/kg).The treatment naive mice received daily oral gavage of 0.2 mL double distilled water and 0.1 mL normal saline via intraperitoneal injection once a *** control group received daily oral gavage of 0.2 mL double-distilled *** treatment period was 30 *** the end of treatment,mice organs were harvested to analyze immunological activities via immunophenotyping,gene and multiplex analysis,histological staining,and gut microbiota *** Mice treated with the combination of 4HF and doxorubicin resulted in significantly reduced tumor and spleen burdens(Pcyte landscape,and manipulated gut microbiota to favor the anti-tumor immunological ***,immunosuppressive genes,cytokines,and chemokines such as c-c motif chemokine 2 and interleukin-10 of tumors were significantly downregulated(Pcin combination treatment demonstrated synergetic activities and was most effective

关键词： adjunctivetherapy breast cancer cytokines chinese medicine tumor microenvironment

Molecular template derived ultrathin N-doped carbon layer on cobalt selenide nanobelts for durable and rapid sodium storage

在线全文

学校读者我要写书评

暂无评论

Nano Research 2024年第9期17卷 8145-8154页

作者： chuanliang Wei Baojuan Xi Kangdong Tian Xinlu Zhang Quanyan man Keyan Bao Wutao Mao Jinkui Feng Shenglin Xiong Key Laboratory of the colloid and Interface chemistry Ministry of EducationSchool of Chemistry and Chemical EngineeringSchool of Materials Science and EngineeringShandong UniversityJinan 250100China Resource Environment and clean Energy Laboratory School of Chemical and Environmental EngineeringJiangsu University of TechnologyChangzhou 213001China

Sodium-ion batteries(SIBs)are an attractive battery system because of similar characteristics to lithium-ion batteries(LIBs)and large Na element ***,exploring stable,high-capacity and high-rate anode materials for SIBs is still challenging ***,diethylenetriamine(DETA)molecular template derived ultrathin N-doped carbon(Nc)layer decorated coSe_(2)nanobelts(coSe_(2)/Nc)are prepared by solvothermal reaction followed by calcination *** coSe_(2)/Nc exhibits large potential as an anode for *** and theoretical calculations reveal that the in situ formed conductive ultrathin Nc layer can not only relieve the volume change of coSe_(2)but also accelerate electron and ion *** addition,the nanobelt structure of coSe_(2)/Nc with abundant exposed active sites can obviously accelerate the electrochemical *** the synergistic effect of special nanobelt structure and Nc layer,the rate as well as cycling performances of coSe_(2)/Nc are obviously improved.A superior capacity retention of 94.8%is achieved at 2 A·g^(-1)after 2000 *** using Na3V2(PO4)3 cathodes,the pouch full batteries can work steadily at 0.5 c,verifying the application ***_(2)/Nc anodes also exhibit impressive performances in LIBs and potassium-ion batteries(PIBs).

关键词： coSe2 nanobelts ultrathin N-doped carbon layer sodium-ion batteries anode high rate

Increased retinal venule diameter as a prognostic indicator for recurrent cerebrovascular events:a prospective observational study

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Neural Regeneration Research 2024年第5期19卷 1156-1160页

作者： Ying Zhao Dawei Dong Ding Yan Bing Yang Weirong Gui man Ke Anding Xu Zefeng Tan Department of Neurology and Stroke center the First Affiliated Hospital of Jinan UniversityGuangzhouGuangdong ProvinceChina clinical Neuroscience Institute the First Affiliated Hospital of Jinan UniversityGuangzhouGuangdong ProvinceChina Department of Neurology the First People’s Hospital of FoshanFoshanGuangdong ProvinceChina

Microvasculature of the retina is considered an alternative marker of cerebral vascular risk in healthy ***,the ability of retinal vasculature changes,specifically focusing on retinal vessel diameter,to predict the recurrence of cerebrovascular events in patients with ischemic stroke has not been determined *** previous studies have shown a link between retinal vessel diameter and recurrent cerebrovascular events,they have not incorporated this information into a predictive ***,this study aimed to investigate the relationship between retinal vessel diameter and subsequent cerebrovascular events in patients with acute ischemic ***,we sought to establish a predictive model by combining retinal veessel diameter with traditional risk *** performed a prospective observational study of 141 patients with acute ischemic stroke who were admitted to the First Affiliated Hospital of Jinan *** of these patients underwent digital retinal imaging within 72 hours of admission and were followed up for 3 *** found that,after adjusting for related risk factors,patients with acute ischemic stroke with mean arteriolar diameter within 0.5-1.0 disc diameters of the disc margin(MAD_(0.5-1.0DD))of≥74.14μm and mean venular diameter within 0.5-1.0 disc diameters of the disc margin(MVD_(0.5-1.0DD))of≥83.91μm tended to experience recurrent cerebrovascular *** established three multivariate cox proportional hazard regression models:model 1 included traditional risk factors,model 2 added MAD_(0.5-1.0DD)to model 1,and model 3 added MVD0.5-1.0DD to model *** 3 had the greatest potential to predict subsequent cerebrovascular events,followed by model 2,and finally model *** findings indicate that combining retinal venular or arteriolar diameter with traditional risk factors could improve the prediction of recurrent cerebrovascular events in patients with acute ischemic stroke,and that retinal imaging c

关键词： acute ischemic stroke arteriolar cerebrovascular events diameter digital retinal imaging microvasculature prediction recurrent retina venular

Jet modification in absence of QGP-medium:the role of multiparton interactions and color reconnection

在线全文

学校读者我要写书评

暂无评论

chinese Physics c 2024年第1期48卷 50-60页

作者： Prottoy Das Abhi Modak Debjani Banerjee Rathijit Biswas Supriya Das Sanjay KGhosh Sibaji raha Sidharth Kumar Prasad Department of Physics Bose InstituteKolkata 700091India Quark Matter Research center Institute of Modern PhysicsChinese Academy of SciencesLanzhou 730000China

Recent studies on high-multiplicity events in small collision systems(proton-proton and proton-lead)have drawn considerable research interest toward the possibility of the formation of partonic medium in such *** of the important consequences of the formation of dense partonic medium is the quenching of high-momentum final-state particles,resulting in several experimental observations such as suppression in nuclear modification factor RAA,modification of jet shape observableρ(r)and jet fragmentation(z^(ch))distributions,*** this work,we studyρ(r)and z^(ch)for inclusive charged-particle jets in proton-proton(pp)collisions at√s=13 TeV using the PYTHIA 8 Monash 2013 Monte carlo *** show that the color reconnection(cR)and multiparton interaction(MPI)mechanisms in PYTHIA 8 can lead to an increased rate of jet *** also find that the mechanisms of MPI and cR and change in the gluonic contribution in high-multiplicity events result in significant modification ofρ(r)and z^(ch)compared to those in minimum bias events for 10ch)ce a direct connection ofand gluonic contribution with the amount of modification inρ(r):the larger the number of MPIs and/or gluonic contribution,the larger the amount of modification ofρ(r).

关键词： jet modification PYTHIA multiparton interaction color reconnection gluonic contribution high-multiplicity

Wild soybean(Glycine soja)transcription factor GsWRKY40 plays positive roles in plant salt tolerance

在线全文

学校读者我要写书评

暂无评论

The crop Journal 2024年第3期12卷 766-775页

作者： Minglong Li man Xue Huiying Ma Peng Feng Tong chen Xiaohuan Sun Qiang Li Xiaodong Ding Shuzhen Zhang Jialei Xiao Key Laboratory of Agricultural Biological Functional Genes Northeast Agricultural UniversityHarbin 150030HeilongjiangChina Key Laboratory of Soybean Biology of chinese Education Ministry Northeast Agricultural UniversityHarbin 150030HeilongjiangChina Department of Plant Science School of Agriculture and BiologyShanghai Jiao Tong UniversityShanghai 200240China college of Life Science Leshan Normal UniversityLeshan 614000SichuanChina Mudanjiang Branch of Heilongjiang Academy of Agricultural Sciences Mudanjiang 157401HeilongjiangChina

Wild soybean(Glycine soja),a relative of cultivated soybean,shows high adaptability to adverse environmental *** identified and characterized a wild soybean transcription factor gene,GsWRKY40,that promotes plant salt ***40 was highly expressed in wild soybean roots and was up-regulated by salt ***40 was localized in nucleus and demonstrated DNA-binding activities but without transcriptional *** and overexpression of GsWRKY40 altered salt tolerance of Arabidopsis *** understand the molecular mechanism of GsWRKY40 in regulating plant salt resistance,we screened a cDNA library and identified a GsWRKY40 interacting protein GsbHLH92 by using yeast two-hybrid *** physical interaction of GsWRKY40 and GsbHLH92 was confirmed by co-immunoprecipitation(co-IP),GST pull-down,and bimolecular fluorescence complementation(BiFc)***,co-overexpression of GsWRKY40 and GsbHLH92 resulted in higher salt tolerance and lower ROS levels than overexpression of GsWRKY40 or GsbHLH92 in composite soybean plants,suggesting that GsWRKY40 and GsbHLH92 may synergistically regulate plant salt resistance through inhibiting ROS ***-PcR data indicated that the expression level of GmSPOD1 gene encoding peroxidase was cooperatively regulated by GsWRKY40 and GsbHLH92,which was confirmed by using a dual luciferase report system and yeast one-hybrid *** study reveals a pathway that GsWRKY40 and GsbHLH92 collaboratively up-regulate plant salt resistance through impeding GmSPOD1 expression and reducing ROS levels,providing a novel perspective on the regulatory mechanisms underlying plant tolerance to abiotic stresses.

关键词： Wild soybean Transcription factor Salt stress ROS

Diverse nucleotide substitutions in rice base editing mediated by novel TadA variants

在线全文

学校读者我要写书评

暂无评论

Plant communications 2024年第8期5卷 104-112页

作者： man Yu Yongjie Kuang chenyang Wang Xuemei Wu Shaofang Li Dawei Zhang Wenxian Sun Xueping Zhou Bin Ren Huanbin Zhou State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100193China Department of Plant Pathology China Agricultural UniversityBeijing 100193China Scientific Observing and Experimental Station of crop Pests in Guilin Ministry of Agriculture and Rural AffairsGuilin 541399China Ministry of Education Key Laboratory for Bio-Resource and Eco-Environment College of Life ScienceState Key Laboratory of Hydraulics and Mountain River EngineeringSichuan UniversityChengdu 610064China State Key Laboratory of Vegetable Biobreeding National Engineering Research Center for VegetablesBeijing Vegetable Research CenterBeijing Academy of Agriculture and Forestry ScienceBeijing 100097China State Key Laboratory of Rice Biology Institute of BiotechnologyZhejiang UniversityHangzhou 310058China Key Laboratory of Gene Editing Technologies(Hainan) Ministry of Agricultural and Rural AffairsSanya 572024China

cRISPR-mediated base editors have been widely used to correct defective alleles and create novel alleles by artificial evolution for the rapid genetic improvement of *** editing capabilities of base editors strictly rely on the performance of various nucleotide modification *** with the welldeveloped adenine base editors(ABEs),cytosine base editors(cBEs)and dual base editors suffer from unstable editing efficiency and patterns at different genomic loci in rice,significantly limiting their ***,we comprehensively examined the base editing activities of multiple evolved TadA8e variants in *** found that both TadA-cDd and TadA-E27R/N46L achieved more robust c-to-T editing than previously reported hyperactive hAID*D,and TadA-cDd outperformed TadA-E27R/N46L.A c-to-G base editor(cGBE)engineered with TadA-cDd and OsUNG performed highly efficient c-to-G editing in rice compared with that of *** addition,a dual base editor constructed with a single protein,TadDE,enabled simultaneous,highly efficient c-to-T and A-to-G editing in ***,our results demonstrate that TadA8e derivatives improve both cBEs and dual base editors in rice,providing a powerful way to induce diverse nucleotide substitutions for plant genome editing.

关键词： cRISPR TadA variants cytosine base editing dual base editor rice

Photo-induced crosslinked and anti-PD-L1 peptide incorporated liposomes to promote PD-L1 multivalent binding for effective immune checkpoint blockade therapy

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Acta Pharmaceutica Sinica B 2024年第3期14卷 1428-1440页

作者： Youngjoo Lee Sukyung Song Suah Yang Jinseong Kim Yujeong Moon Nayeon Shim Hong Yeol Yoon Sehoon Kim man Kyu Shim Kwangmeyung Kim KU-KIST Graduate School of converging Science and Technology Korea UniversitySeoul 02841Republic of Korea Medicinal Materials Research center Biomedical Research DivisionKorea Institute of Science and Technology(KIST)Seoul 02792Republic of Korea college of Pharmacy Graduate School of Pharmaceutical SciencesEwha Womans UniversitySeoul 03760Republic of Korea Department of Bioengineering Korea UniversitySeoul 02841Republic of Korea

Immune checkpoint blockade(IcB)therapy targeting PD-L1 via monoclonal antibody(m Ab)has shown extensive clinical benefits in the diverse types of advanced ***,most patients are completely refractory to IcB therapy owing to the PD-L1 recycling ***,we propose photo-induced crosslinked and anti-PD-L1 peptide incorporated liposomes(immune checkpoint blockade liposomes;IcB-LPs)to promote PD-L1 multivalent binding for inducing lysosomal degradation of PD-L1 in tumor *** IcB-LPs are prepared by formulation of Dc_(8,9)Pc with photo-polymerized diacetylenic moiety,1,2-dipalmitoylphosphatidylcholine(DPPc)and anti-PD-L1peptide(D-form NYSKPTDRQYHF)-conjugated DSPE-PEG_(2k)(anti-PD-L1-DSPE-PEG_(2k))in a molar ratio of 45:45:10,followed by cross-linking of liposomal bilayer upon UV *** 10 mol% antiPD-L1-DSPE-PEG_(2k)incorporated IcB-LPs have a nano-sized lipid bilayer structure with an average diameter of 137.7±1.04 nm,showing a high stability in serum ***,the IcB-LPs efficiently promote the multivalent binding with PD-L1 on the tumor cell membrane,which are endocytosed with aim to deliver PD-L1 to the lysosomes,wherein the durable PD-L1 degradation is observed for72 h,in contrast to anti PD-L1 m Abs showing the rapid PD-L1 recycling within 9 *** in vitro coculture experiments with cD8^(+)T cells show that IcB-LPs effectively enhance the T cell-mediated antitumor immune responses against tumor cells by blocking the PD-L1/PD-1 *** IcB-LPs are intravenously injected into colon tumor-bearing mice,they efficiently accumulate within the targeted tumor tissues via both passive and active tumor targeting,inducing a potent T cell-mediated antitumor immune response by effective and durable PD-L1 ***,this study demonstrates the superior antitumor efficacy of crosslinked and anti-PD-L1 peptide incorporated liposome formulation that promotes PD-L1 multivalent binding for trafficking of PD-L1 toward

关键词： cancer immunotherapy Immune checkpoint blockade PEGylated liposome crosslinked lipid nanoparticles Anti-PD-L1 peptide Tumor-targeting PD-L1 multivalent binding Lysosomal PD-L1 degradation

3'-Deoxyadenosin alleviates methamphetamine-induced aberrant synaptic plasticity and seeking behavior by inhibiting the NLRP3 inflammasome

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2024年第10期19卷 2270-2280页

作者： Yize Qi Yao Zhou Jiyang Li Fangyuan Zhu Gengni Guo can Wang man Yu Yijie Wang Tengfei Ma Shanwu Feng Li Zhou Institute for Stem cell and Neural Regeneration and Key Laboratory of cardiovascular&cerebrovascular Medicine School of PharmacyNanjing Medical UniversityNanjingJiangsu ProvinceChina Department of Anesthesiology The Second People’s Hospital of LianyungangLianyungangJiangsu ProvinceChina Department of Anesthesiology Women’s Hospital of Nanjing Medical UniversityNanjing Maternity and Child Health Care HospitalNanjingJiangsu ProvinceChina

Methamphetamine addiction is a brain disorder characterized by persistent drug-seeking behavior, which has been linked with aberrant synaptic plasticity. An increasing body of evidence suggests that aberrant synaptic plasticity is associated with the activation of the NOD-like receptor family pyrin domain containing-3(NLRP3) inflammasome. 3′-Deoxyadenosin, an active component of the chinese fungus cordyceps militaris, has strong anti-inflammatory effects. However, whether 3′-deoxyadenosin attenuates methamphetamine-induced aberrant synaptic plasticity via an NLRP3-mediated inflammatory mechanism remains unclear. We first observed that 3′-deoxyadenosin attenuated conditioned place preference scores in methamphetamine-treated mice and decreased the expression of c-fos in hippocampal neurons. Furthermore, we found that 3′-deoxyadenosin reduced the aberrant potentiation of glutamatergic transmission and restored the methamphetamine-induced impairment of synaptic plasticity. We also found that 3′-deoxyadenosin decreased the expression of NLRP3 and neuronal injury. Importantly, a direct NLRP3 deficiency reduced methamphetamine-induced seeking behavior, attenuated the impaired synaptic plasticity, and prevented neuronal damage. Finally, NLRP3 activation reversed the effect of 3′-deoxyadenosin on behavior and synaptic plasticity, suggesting that the anti-neuroinflammatory mechanism of 3′-deoxyadenosin on aberrant synaptic plasticity reduces methamphetamine-induced seeking behavior. Taken together, 3′-deoxyadenosin alleviates methamphetamine-induced aberrant synaptic plasticity and seeking behavior by inhibiting the NLRP3 inflammasome.

关键词： 3′-deoxyadenosin hippocampus long-term potentiation methamphetamine NOD-like receptor family pyrin domain containing-3(NLRP3)inflammasome synaptic plasticity