检索结果-南通市图书馆

End-to-end computational approach to the design of rNA biosensors for detecting mirNA biomarkers of cervical cancer

Synthetic and Systems Biotechnology 2022年第2期7卷 802-814页

作者： Priyannth ramasami S.Baabu Shivaramakrishna Srinivasan Swetha Nagarajan Sangeetha Muthamilselvan Thamarai Selvi raghavv r.suresh Ashok Palaniappan Department of Bioinformatics School of Chemical and BiotechnologySASTRA Deemed UniversityThanjavurTamil Nadu 613401India Department of Bioengineering School of Chemical and BiotechnologySASTRA Deemed UniversityThanjavurTamil Nadu613401India School of Advanced Materials Science and Engineering Sungkyunkwan University(SKKU)Suwon16419Republic of Korea Life Sciences Division TCS ResearchTata Consultancy ServicesChennaiTamil Nadu600042India

Cervical cancer is a global public health subject as it affects women in the reproductive ages,and accounts for the second largest burden among cancer patients worldwide with an unforgiving 50%mortality *** scant awareness and limited access to effective diagnosis have led to this enormous disease burden,calling for point-of-care,minimally invasive diagnosis ***,an end-to-end quantitative unified pipeline for diagnosis has been developed,beginning with identification of optimal biomarkers,concurrent design of toehold switch sensors,and finally simulation of the designed diagnostic circuits to assess *** mirNA expression data in the public domain,we identified mir-21-5p and mir-20a-5p as blood-based mirNA biomarkers specific to early-stage cervical cancer employing a multi-tier algorithmic *** riboregulators called toehold switches specific to the biomarker panel were then *** predict the dynamic range of toehold switches for use in genetic circuits as biosensors,we used a generic grammar of these switches,and built a neural network model of dynamic range using thermodynamic features derived from mrNA secondary structure and ***-generation toehold switches were used to overcome the design challenges associated with mirNA *** resultant model yielded an adj.r^(2)~0.71,outperforming earlier models of toehold-switch dynamic *** kinetics modelling was performed to predict the sensitivity of the second-generation toehold switches to the mirNA *** showed a linear response between 10 nM and 100 nM before *** study demonstrates an end-to-end computational workflow for the efficient design of genetic circuits geared towards the effective detection of unique genomic/nucleic-acid *** approach has the potential to replace iterative experimental trial and error,and focus time,money,and *** software including the toehold grammar parser,neu

关键词： Cervical cancer biosensor mirNA biomarker Synthetic toehold switches Genetic circuit design reaction network modelling Toehold efficacy modelling Toehold switch grammar Machine learning

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：