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Effects of CXCL12 isoforms in a pancreatic pre-tumour cellular model:Microarray analysis

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World Journal of Gastroenterology 2021年第15期27卷 1616-1629页

作者： Monia Cecati Matteo Giulietti Alessandra righetti Berina Sabanovic Francesco Piva Department of Specialistic Clinical and Odontostomatological Sciences Polytechnic University of MarcheAncona 60126Italy

BACKGROUND Pancreatic ductal adenocarcinoma(PDAC)is the fourth leading cause of death among cancers,it is characterized by poor prognosis and strong *** the PDAC microenvironment,stromal cells release different extracellular components,including *** CXCL12 is a chemokine promoting the communication between tumour and stromal *** different splicing isoforms of CXCL12 are known(α,β,γ,δ,ε,θ)but their role in PDAC has not yet been *** To investigate the specific role ofα,β,andγCXCL12 isoforms in PDAC *** We used hTERT-HPNE E6/E7/KRasG12D(Human Pancreatic Nestin-Expressing)cell line as a pancreatic pre-tumour model and exposed it to theα,β,andγCXCL12 *** altered expression profiles were assessed by microarray analyses and confirmed by Real-Time polymerase chain *** functional enrichment analyses have been performed by Enrichr tool to highlight Gene Ontology enriched *** addition,wound healing assays have been carried out to assess the phenotypic changes,in terms of migration ability,induced by theα,β,andγCXCL12 *** Microarray analysis of hTERT-HPNE cells treated with the three different CXCL12 isoforms highlighted that the expression of only a few genes was ***,theαandβisoforms showed an alteration in expression of different genes,whereasγisoform affected the expression of genes also common withαandβ***βisoform altered the expression of genes mainly involved in cell cycle *** addition,all isoforms affected the expression of genes assay confirmed that CXCL12 enhanced the migration ability of hTERT-HPNE *** the CXCL12 splicing isoforms,theγisoform showed higher induction of migration thanαandβ*** Our data suggests an involvement and different roles of CXCL12 isoforms in PDAC ***,more investigations are needed to confirm these preliminary observations.

关键词： CXCL12 Splicing isoforms Pancreatic cancer Microarray Migration Wound healing assay

Using Vetiver grass technology for mitigating sediment loads in the Talakhaya Watershed areas in Rota, CNMI

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International Soil and Water Conservation Research 2018年第2期6卷 194-201页

作者： Mohammad HGolabi Sydonia Manibusan Timothy righetti Dana Okano Clancy Iyekar University of Guam USA National Oceanic and Atmospheric Administration CNMIUSA

Talakhaya watershed in Rota is identified as a Coral Reef Management Priority site for CNMI (Com-monwealth of Northern Mariana Islands). In 2010 federal and jurisdictional partners developed a Con-servation Action Plan (CAP) for the Talakhaya Watershed. The goal of this initial Watershed Soil Loss Assessment therefore, was to assist in evaluating the re-vegetation objectives of 'Mitigating Sediment Load' in the Talakhaya watershed by making a thorough characterization of its main river systems. The objectives of this project were to reach those goals by measuring the hydrological parameters following the installation and the use of water meters, barometric level loggers, turbidity meters and rain gauges. The water flow as well as the turbidity level of each stream leading to the ocean from the Talakhaya Watershed was measured and sedimentation level was assessed accordingly. The results from the monitoring of the watershed conducted during the project timeline reported here show that all four rivers under study have statistically different stage-discharge curves. The river's relationship between time and turbidity also vary especially in the summer months of the first-year observations. Linear and convex polynomial relationships were sometimes observed in the different rainfall groupings for the four river systems, however relationships were often not significant. This-being-said, Quantile Regressions suggest that when data from all four river systems are combined for cases where there is some de-tectable rainfall, a maximum possible turbidity level can be defined.

关键词： Watershed Management Vetiver grass system Coral Badlands Micronesia Rota CNMI

P2X7 receptor antagonist recovers ileum myenteric neurons after experimental ulcerative colitis

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World Journal of Gastrointestinal Pathophysiology 2020年第4期11卷 84-103页

作者： Roberta Figueiroa Souza Mariá Munhoz Evangelinellis Cristina Eusébio Mendes Marta righetti Múcio Cevulla Silva Lourenco Patricia Castelucci Department of Anatomy University of Sao PauloSao Paulo 05508-900Brazil Department of Surgery Faculty of Veterinary Medicine and Animal ScienceUniversity of Sao PauloSao Paulo 05508-900Brazil

BACKGROUND The P2X7 receptor is expressed by enteric neurons and enteric glial *** have demonstrated that administration of a P2X7 receptor antagonist,brilliant blue G(BBG),prevents neuronal *** To report the effects of BBG in ileum enteric neurons immunoreactive(ir)following experimental ulcerative colitis in Rattus norvegicus *** 2,4,6-trinitrobenzene sulfonic acid(TNBS group,n=5)was injected into the distal ***(50 mg/kg,BBG group,n=5)or vehicle(sham group,n=5)was given subcutaneously 1 h after *** animals were euthanized after 24 h,and the ileum was *** was performed on the myenteric plexus to evaluate immunoreactivity for P2X7 receptor,neuronal nitric oxide synthase(nNOS),choline acetyltransferase(ChAT),HuC/D and glial fibrillary acidic *** The numbers of nNOS-,ChAT-,HuC/D-ir neurons and glial fibrillary acidic protein-ir glial cells were decreased in the TNBS group and recovered in the BBG *** neuronal profile area(μm^2)demonstrated that nNOS-ir neurons decreased in the TNBS group and recovered in the BBG *** were no differences in the profile areas of ChAT-and HuC/D-ir *** Our data conclude that ileum myenteric neurons and glial cells were affected by ulcerative colitis and that treatment with BBG had a neuroprotective ***,these results demonstrate that the P2X7 receptor may be an important target in therapeutic strategies.

关键词： P2X7 receptor Brilliant blue G Myenteric plexus Experimental ulcerative colitis Ileum Chemical coding

Differential IL10 mRNA Profiles Associated to <i>Babesia bovis</i>and <i>B. bigemina</i>Infection Levels in Persistently Infected Animals

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Open Journal of Veterinary Medicine 2019年第11期9卷 161-169页

作者： Talita Barban Bilhassi Rodrigo Giglioti Cintia Hiromi Okino Wilson Malagó Júnior Henrique Nunes de Oliveira Cíntia righetti Marcondes Márcia Cristina de Sena Oliveira Universidade Estadual Paulista Júlio de Mesquita Filho UNESP/FCAV Campus de Jaboticabal Jaboticabal SP Brazil Embrapa Pecuária Sudeste São Carlos SP Brazil Institute of Animal Science (APTA-SAA) Nova Odessa SP Brazil

This work aimed to find quantitative phenotypic traits that can be used to discriminate the levels of resistance/susceptibility to B. bovis and B. bigemina in two groups of cattle presenting the highest (H) or lowest (L) infection levels and Rhipicephalus microplus ticks count. The animals were selected from a previous study of 50 Canchim (5/8 Charolais/zebu) heifers raised in an endemic area for these parasites. These animals were evaluated regarding their TNFα, IL10, IFN-γ, IL12 and iNOS mRNA levels. No differences were found between these groups regarding TNFα, IFN-γ, IL12β or iNOS transcripts. However, the IL10 transcripts were significantly higher in the H group compared to the L group. Moreover, significant correlation coefficients were observed between B. bovis loads and both IL10 and IFN-γ transcripts, while no correlations were found for B. bigemina loads and all tested immune-related transcripts, suggesting that differential IL10 mRNA profiles were closely associated to B. bovis loads. Our results have contributed to a better understanding of the immune responses against Babesia infection, as we demonstrated that the IL10 cytokine levels might also influence or be influenced by parasitemia levels in persistently infected animals.

关键词： Babesia bovis Cytokines Gene Expression IL10 RT-qPCR

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功能基因组学在鉴别新的卵蛋白质中的应用

中国家禽 2010年第7期32卷 40-44页

作者： J.Gautron K.Mann P.G.righetti S.Réhault-Godbert V.Jonchère V.Hervé-Grepinet Y.Nys 朱静胡娟法国国家农业研究所德国马克斯普朗克研究所生物化学蛋白质组学和信号转导处意大利米兰理工大学

随着分子生物学的发展、鸡基因组序列的公布及新的生物信息学工具的开发,禽蛋中一些以前未被发现的微量成分正被不断大量识别和鉴定出来。本文从生物化学(蛋白质组学)、分子生物学(转录组学)和蛋壳分泌蛋白质组的鉴定等领域阐述了卵蛋... 详细信息

随着分子生物学的发展、鸡基因组序列的公布及新的生物信息学工具的开发,禽蛋中一些以前未被发现的微量成分正被不断大量识别和鉴定出来。本文从生物化学(蛋白质组学)、分子生物学(转录组学)和蛋壳分泌蛋白质组的鉴定等领域阐述了卵蛋白的最新研究进展。

关键词：功能基因组学蛋白质生殖细胞发育应用鉴别生物活性分子胚胎发育营养物质

Anomalies Observed in VLF and LF Radio Signals on the Occasion of the Western Turkey Earthquake(M_(W)=5.7)on May 19,2011

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International Journal of Geosciences 2012年第4期3卷 856-865页

作者： Pier Francesco Biagi Flavia righetti Tommaso Maggipinto Luigi Schiavulli Teresa Ligonzo Anita Ermini Iren Adelina Moldovan Adrian Septimiu Moldovan Hugo Goncalves Silva Mourad Bezzeghoud Michael E.Contadakis Dimitrios N.Arabelos Thomas D.Xenos Aydin Buyuksarac Department of Physics University of BariBariItaly Department of Industrial Engineering University of Tor VergataRomeItaly National Institute of Earth’s Physics Seismological DepartmentBucharestRomania AZEL-Designing Group S.R.L. BucharestRomania Geophysical Centre of Evora and Physics Department ECTUniversity of EvoraEvoraPortugal Department of Surveying&Geodesy University of ThessalonikiThessalonikiGreece Department of Geophysics Canakkale Onsekiz Mart UniversityCanakkaleTurkey

Since 2009 a network of VLF (20 - 60 kHz) and LF (150 - 300 kHz) radio receivers is operating in Europe in order to study the disturbances produced by the earthquakes on the propagation of these signals. In 2011 the network was formed by nine receivers, of which three are located in Italy and one is in Austria, Greece, Portugal, Romania, Russia and Turkey. On May 19, 2001 an earthquake (Mw = 5.7) occurred in western Turkey, that is inside the “sensitive” area of the network. The radio data collected during April-May 2011 were studied using the Wavelet spectra, the Principal Component Analysis and the Standard Deviation trends as different methods of analysis. Evident anomalies were revealed both in the signals broadcasted by the TRT transmitter (180 kHz) located near Ankara and in a VLF signal coming from a transmitter located in Western Europe and collected by the receiver TUR of the network located in eastern Turkey. Evident precursor phases were pointed out. Some differences in the efficiency of the three analysis methods were revealed.

关键词： Earthquake Precursors European Radio Network Radio Signal Analysis

Inflammatory fibroid polyps in children:A new case report and a systematic review of the pediatric literature

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World Journal of Clinical Pediatrics 2015年第4期4卷 160-166页

作者： Laura righetti Filippo Parolini Paolo Cengia Giovanni Boroni Maurizio Cheli Aurelio Sonzogni Daniele Alberti Department of Pediatric Surgery Spedali Civili Hospital Department of Surgery Spedali Civili Hospital Department of Pediatric Surgery Papa Giovanni XXXIII Hospital Department of Pathology Papa Giovanni XXXIII Hospital Department of Pediatric Surgery Spedali Civili HospitalUniversity of Brescia

AIM: To study that inflammatory fibroid polyps(IFPs) in children are extremely uncommon tumors that may occur throughout the gastrointestinal tract. METHODS: A systematic review of the pediatric literature and a report of a new case of IFP is also pres-ented. The Pub Med database was searched for original studies on pediatric IFPs since 1960, according to "Preferred reporting items for systematic reviews and meta-analyses" guidelines for systematic reviews. RESULTS: Five studies were finally enclosed, encompassing 6 children with IFPs(mean age 64 mo). Tumors were located in the stomach(2 patients), in the small bowel(2 patients), in the rectum(1 patient) and in the colon(1 patient). Open surgery was performed in all patients and complete excision of the mass was achieved in all cases. All patients are alive and free of symptom. Authors described a further case of a 3-year-old boy with a large duodenal IFP, in whom the tumor was removed by "en block resection". The presence of IFP throughout the gastrointestinal tract and its variable clinical appearances make it difficult to diagnose. An accurate pre-operative assessment is fundamental in order to differentiate IFP from other more aggressive gastrointestinal tumor, enabling unnecessary demolitive surgery. CONCLUSION: When complete resection of the IFP is achieved, the prognosis is excellent.

关键词： Inflammatory fibroid polyp Duodenum Ultrasound endoscopy Children Surgery