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Sensitivity of a Rapid Mix Test with Combined Synthetic Antigens Derived from Mycobacterium Leprae PGL-1 for Diagnosis and Surveillance of Leprosy

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Open Journal of Immunology 2020年第1期10卷 1-9页

作者： Maria do Perpétuo Socorro Amador Silvestre Maxwell Furtado de Lima Antonio G.P.Pereira Aline Rodrigues Bernardo rafaela lopes diniz Pedro Paulo Ferreira Ribeiro Edimílson Domingos da Silva Luana Nepomuceno Gondim Costa Lima Instituto Evandro Chagas Rodovia BR-316km 07AnanindeuaParáBrasil Programa de Desenvolvimento Tecnologico de reativos para diagnostico GPRED-Bio Manguinhos-FioCruzRio de JaneiroBrasil

Introduction: In the Americas, Brazil contributes 91.63% of the total cases and the state of Pará still has high endemia for leprosy. Objective: To analyze the performance of a rapid test for the diagnosis and epidemiological surveillance of leprosy in endemic areas. Methods: The sample consisted of 70 MB multibacillary leprosy (MB) patients, 63 paucibacillary (PB) patients, and 80 intradomiciliary consanguineous contacts (ICSCO) of patients. A rapid test with a 15-minute reading was applied using two prototypes: prototype 1, double test with trisaccharide antigen (NT-P-BSA) at 1a. line (83.2 ng/test) and disaccharide antigen (ND-O-BSA) at 2a. (83.2 ng/test), both with a flow of 0.08 μL/mm with a 10 μC membrane, anti-IgM conjugate with a flow of 0.040 μL/mm and a Tris-Triton and prototype 2 runner buffer with MIX antigen (trisaccharide + disaccharide) in the same concentrations and conditions of prototype 1. Results: The comparison of the MIX test positivity rate and the disaccharide or trisaccharide doublet test across all samples was statistically significant, demonstrating that the MIX test had higher seropositivity rates compared to the ND-O-BSA or NT-P-BSA. It was demonstrated that the MIX test showed a good performance, with 25.39% of the PB patients negative for the disaccharide and trisaccharide duplet test, but positive for MIX. Conclusions: These data suggest the potential for further optimizing the performance by adding other synthetic antigens to the MIX antigens.

关键词： Leprosy Diagnosis Rapid Test

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Evaluation of calcined textile sludge as a stabilizing material for highway soil

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Journal of Traffic and Transportation Engineering(English Edition) 2020年第5期7卷 688-699页

作者： Alice Gonzaga de Oliveira Amanda diniz Barros Leda Christiane de Figueiredo lopes Lucena Adriano Elisio de Figueiredo lopes Lucena Jonny Dantas Patricio Department of Civil Engineering Federal University of Campina GrandeCampina Grande 58429140Brazil

The textile industry is a pioneer in the industrialization process in Brazil and is relevant to the Brazilian economy. However, it also generates as operational residue a solid waste,commonly called textile sludge. Pavement construction, which mobilizes large volumes of soil, constitutes an alternative to the reuse of residue, especially when stabilization is involved. The use of stabilized soils with the addition of waste can provide technical,economic and environmentally friendly advantages. This research aimed to study the use of chemical stabilization technique with sludge and three additives(lime, cement and asphalt emulsion) applied in base and subbase of pavements. The scope of this study was divided into four phases, i) characterization tests, ii) physical stabilization, iii) chemical stabilization and determination of heavy metals in the sludge, and iv) addition of 10% of textile sludge and chemical stabilizers(lime, cement, and asphalt emulsion) in amounts of 3%, 5% and 7% as additives in a soil, based on the results obtained in the second phase. Test results indicated that the stabilization of mixtures of soil with textile sludge has the potential to be used in pavement layers(base and subbase). The addition of cement promotes the best chemical stabilization for the sludge. The use of textile sludge stabilization presents greater environmental benefits, given the malfunctions and problems that the incorrect waste disposal can cause to the environment.

关键词： Road engineering Soil stabilization Additives Textile sludge Pavement materials Chemical stabilization

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Noninvasive diagnosis of periportal fibrosis in schistosomiasis mansoni:A comprehensive review

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World Journal of Hepatology 2022年第4期14卷 696-707页

作者： Joelma Carvalho Santos Caroline Louise diniz Pereira Ana Lúcia Coutinho Domingues Edmundo Pessoa lopes Postgraduate Program in Tropical Medicine Center of Health SciencesUniversidade Federal de PernambucoRecife 50670-901PernambucoBrazil Gastroenterology Division Department of Internal Medicine of Center of Health SciencesHospital das Clínicas-Universidade Federal de PernambucoRecife 50670-901PernambucoBrazil

Schistosomiasis mansoni is a neglected disease and key public health problem,mainly due to its high prevalence,the scarcity of public policies,and the severity of some clinical *** fibrosis(PPF)is the commonest complication of chronic schistosomiasis mansoni and its diagnosis requires different *** though wedge biopsy of the liver is considered the gold standard,it is not justified in non-surgical patients,and percutaneous liver biopsy may be informative but does not have sufficient *** PPF tests mostly include biological(serum biomarkers or combined scores)or physical assessments(imaging assessment of fibrosis pattern or tissue stiffness).Moreover,imaging techniques,such as ultrasound,computed tomography,magnetic resonance imaging,and elastography are applied not only to support the diagnosis of schistosomiasis,but also to assess and detect signs of portal hypertension and organ damage due to chronic schistosomiasis.A combination between a comprehensive history and physical examination with biomarkers for liver fibrosis and imaging methods seems to offer the best approach for evaluating these *** addition,understanding their strengths and limitations will allow a more accurate interpretation in the clinical context and can lead to greater accuracy in estimating the degree of fibrosis in patients with Schistosomiasis mansoni(***)*** review will discuss the different noninvasive methods that are currently available for the evaluation of PPF in *** infection,and their application,advantages,and limitations in clinical practice.

关键词： Schistosoma mansoni Parasitic liver diseases Liver fibrosis Biomarkers Elasticity imaging techniques Ultrasonography

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Event-based hydrology and sedimentation in paired watersheds under commercial eucalyptus and grasslands in the Brazilian Pampa biome

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International Soil and Water Conservation Research 2021年第2期9卷 180-194页

作者： Ederson diniz Ebling Jose Miguel Reichert Jhon Jairo Zuluaga Pelaez Miriam Fernanda Rodrigues Mirian Lago Valente Rosane Barbosa lopes Cavalcante Paolo Reggiani Raghavan Srinivasan Soils Department Universidade Federal de Santa MariaAvenida Roraima1000 Bairro CamobiSanta MariaRS97105-900Brazil Corporacion Colombiana de Investigacion Agropecuaria(AGROSAVIA) Centro de Investigacion NataimaEspinalColombia CMPC Celulose Riograndense Rua Sao Geraldo1680GuaibaRS92500-000Brazil Department of Civil Engineering University of SiegenPaul Bonatz Strasse 9-11Siegen D57068Germany Department of Ecology and Conservation Biology Texas A&M University(TAMU)534 John Kimbrough Blvd.Room 305College StationTX77843-2120USA Current address:Instituto Tecnologico Vale.Rua Boaventura da Silva 955UmarizalBelemPABrazil.

Ecological and hydrological impacts are expected when watersheds are under commercial *** this study we evaluated event-based hydrology and sedimentation in paired watersheds under commercial eucalyptus and grasslands ***,suspended sediment concentration,and sediment production were measured and simulated with the Limburg Soil Erosion Model(LISEM)in paired hydrographic watersheds located in southern Brazil;one occupied with 7-years old Eucalyptus saligna plantation(forest watershed-FW;0.83 km^(2))and another Pampa biome with native and exotic grasses used for beef cattle production(grassland watershed-GW;1.10 km^(2)).The effect of rainfall on hydrological and sedimentological variables was more evident in GW compared with *** shape of the hydrograph was steepest in GW,with rapid,marked increase and decrease in flow during *** parameters changed for model calibration in FW and GW were soil saturated hydraulic conductivity,initial soil moisture,and Manning roughness coefficient,whereby the statistical performance was classified as good(NSE>0.75).Fourteen events different from those used in the calibration process were validated for FW and GW,with NSE index classified as“good”and“very good”for *** study shows limitations of LISEM in calibrating and validating the sediment yield for individual events due to the spatial variability of factors controlling soil *** validated modeling results of this study may be applied in simulating soil and crop scenarios in watersheds cultivated with commercial forest and grassland for grazing.

关键词： Southern grasslands Paired watersheds Runoff Erosion LISEM

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Assessment of periportal fibrosis in Schistosomiasis mansoni patients by proton nuclear magnetic resonance-based metabonomics models

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World Journal of Hepatology 2022年第4期14卷 719-728页

作者： Milena Lima Rodrigues Tatiane Priscila Santos Rodrigues da Luz Caroline Louise diniz Pereira Andrea Dória Batista Ana Lúcia Coutinho Domingues Ricardo Oliveira Silva Edmundo Pessoa lopes Programa de Pós-Graduação em Medicina Tropical Centro de Ciências MédicasUniversidade Federal de PernambucoRecife 50670-901PernambucoBrazil Programa de Pós-Graduação em Química Centro de Ciências Exatas e da NaturezaUniversidade Federal de PernambucoRecife 50670-740PernambucoBrazil Hospital das Clínicas Departamento de Medicina ClínicaUniversidade Federal de PernambucoRecife 50670-901PernambucoBrazil

BACKGROUND The evaluation of periportal fibrosis(PPF)is essential for a prognostic assessment of patients with Schistosomiasis *** WHO Niamey Protocol defines patterns of fibrosis from abdominal ultrasonography,^(1)H-nuclear magnetic resonance(NMR)-based metabonomics has been employed to assess liver fibrosis in some *** To build 1H-NMR-based metabonomics models(MM)to discriminate mild from significant periportal PPF and identify differences in the metabolite *** A prospective cross-sectional study was performed on schistosomiasis patients at a University Hospital in Northeastern *** evaluated 41 serum samples from 10 patients with mild PPF(C Niamey pattern)and 31 patients with significant PPF(D/E/F Niamey patterns).MM were built using partial least squares-discriminant analysis(PLS-DA)and orthogonal projections to latent structures discriminant analysis(OPLS-DA)*** PLS-DA and OPLS-DA resulted in discrimination between mild and significant PPF groups with R2 and Q2 values of 0.80 and 0.38 and 0.72 and 0.42 for each model,*** OPLS-DA model presented accuracy,sensitivity,and specificity values of 92.7%,90.3%,and 100%to discriminate significant *** metabolites identified as responsible by discrimination were:Nacetylglucosamines,alanine,glycolaldehyde,carbohydrates,and *** MMs discriminated mild from significant PPF patterns in patients with Schistosomiasis mansoni through identification of differences in serum metabolites profiles.

关键词： Metabolomics Portal hypertension Schistosoma mansoni Biomarkers Neglected disease Nuclear magnetic resonance

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Electrical cardioversion for supraventricular arrhythmias in octagenarians

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Journal of Geriatric Cardiology 2019年第12期16卷 902-904页

作者： Felipe L.Malafaia Patrícia O.Guimarães Márcio C.Sampaio Pedro G.M.de Barros e Silva Renato D.lopes Juliano V.Custódio Miguel da S.diniz Jose C.T.Garcia Valter Furlan Samaritano Paulista Hospital São PauloBrazil Duke Clinical Research Institute Duke UniversityDurhamNCUnited States

Atrial fibrillation(AF)is the most common arrhythmia in the elderly and its incidence increases with aging.[1]Electrical cardioversion is used as a strategy for rhythm control in patients with supraventricular *** octagenarians,there are challenges associated with the indication for electrical cardioversion,such as the use of appropriate sedation and anticoagulant *** decisions in clinical practice for these patients derive from observational data and personal experience,since octagenarians represent a minority of patients included in randomized clinical *** evidence is available regarding the clinical context of electrical cardioversion in this vulnerable ***,we aimed to describe the clinical profile of octagenarians undergoing electrical cardioversion in a cardiology reference center from 2014 to 2018.

关键词： Atrial fibrillation Electrical cardioversion Octagenarians

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Metabolic-associated fatty liver disease is associated with low muscle mass and strength in patients with chronic hepatitis B

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World Journal of Hepatology 2022年第8期14卷 1652-1666页

作者： Cecy Maria de Lima Santos Matheus Duarte Brito Pedro Alves Soares Vaz de Castro Thais Pontello de Vries Nataly lopes Viana Marta Paula Pereira Coelho Olívio Brito Malheiro Tatiana Bering Maria Cristina Gonzalez Rosangela Teixeira Rodrigo Dias Cambraia Gifone Aguiar Rocha Luciana diniz Silva Sciences Applied to Adult Health Care Post-Graduate Programme Faculdade de Medicina Universidade Federal de Minas GeraisBelo Horizonte 30130-100Minas GeraisBrazil Outpatient Clinic of Viral Hepatitis Instituto Alfa de GastroenterologiaFaculdade de MedicinaUniversidade Federal de Minas GeraisBelo Horizonte 30130-100Minas GeraisBrazil Faculdade de Medicina Universidade Federal de Minas GeraisBelo Horizonte 30130-100Minas GeraisBrazil Department of Locomotor System Faculdade de MedicinaUniversidade Federal de Minas GeraisBelo Horizonte 30130-100Minas GeraisBrazil Department of Food and Nutrition Universidade Federal de Mato GrossoCuiabá78060-900Mato GrossoBrazil Postgraduate Program in Health and Behaviour Catholic University of PelotasPelotas 96015-560Rio Grande do SulBrazil Laboratory of Research in Bacteriology Faculdade de MedicinaUniversidade Federal de Minas GeraisBelo Horizonte 30130-100Minas GeraisBrazil Department of Internal Medicine Universidade Federal de Minas GeraisBelo Horizonte 30130-100Minas GeraisBrazil

BACKGROUND Although the prognostic relevance of sarcopenia has been increasingly recognised in the context of liver disease,there is a paucity of data evaluating body composition in patients with chronic hepatitis B(CHB).Beyond virus-related factors,nutritional and metabolic aspects can be associated with skeletal muscle abnormalities in these patients and should not be *** To evaluate the association between components of sarcopenia and demographic,clinical,lifestyle,nutritional,and biochemical variables in CHB *** Dual-energy X-ray absorptiometry(DXA)was used to assess muscle mass by quantifying appendicular lean mass(ALM)adjusted for body mass index(ALMBMI).Muscle function was evaluated by hand grip strength(HGS)and the timed up and go ***-associated fatty liver disease(MAFLD)was defined according to the criteria proposed by an international expert panel.A body shape index and the International Physical Activity Questionnaire were used to assess central obesity and physical activity level,*** This cross-sectional study included 105 CHB outpatients followed at the tertiary care ambulatory centre(mean age,48.5±12.0 years;58.1%males;76.2%without cirrhosis;23.8%with compensated cirrhosis).The DXA-derived fat mass percentage was inversely correlated with the ALMBMI(r=-0.87)and HGS(r=-0.63).In the multivariable analysis,MAFLD,sedentarism and central obesity were positively and independently associated with low *** and central obesity were independently associated with low *** MAFLD and central obesity were associated with low muscle mass and strength in patients with chronic hepatitis B,independent of the liver disease stage.

关键词： Chronic hepatitis B Appendicular lean mass Muscle strength Metabolic associated fatty liver disease Central obesity Physical performance

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Interleukin-6-174G/C polymorphism is associated with a decreased risk of type 2 diabetes in patients with chronic hepatitis C virus

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World Journal of Hepatology 2020年第4期12卷 137-148页

作者： Cliviany Borges da Silva Diego Alves Vieira Luisa Freitas de Melo Anna Luiza Soares Chagas Adriana Dias Gomes César Lúcio lopes de Faria Jr Rosangela Teixeira Dulciene Maria de Magalh?es Queiroz Gifone Aguiar Rocha Maria Marta Sarquis Soares Juliana Maria Trindade Bezerra Luciana diniz Silva Sciences Applied to Adult Health Care Post-Graduate Programme Faculdade de MedicinaUniversidade Federal de Minas GeraisBelo Horizonte 30130100Minas GeraisBrazil Outpatient Clinic of Viral Hepatitis Instituto Alfa de GastroenterologiaFaculdade de MedicinaUniversidade Federal de Minas GeraisBelo Horizonte 30130100Minas GeraisBrazil Medical undergraduate student Faculdade de MedicinaUniversidade Federal de Minas GeraisBelo Horizonte 30130100Minas GeraisBrazil Laboratory of Research in Bacteriology Faculdade de MedicinaUniversidade Federal de Minas GeraisBelo Horizonte 30130100Minas GeraisBrazil Department of Internal Medicine Faculdade de MedicinaUniversidade Federal de Minas GeraisBelo Horizonte 30130100Minas GeraisBrazil Division of Endocrinology Department of Internal MedicineFaculdade de MedicinaUniversidade Federal de Minas GeraisBelo Horizonte 30130100Minas GeraisBrazil Epidemiology of Infectious and Parasitic Diseases Laboratory Department of ParasitologyInstitute of Biological SciencesUniversidade Federal de Minas GeraisBelo Horizonte 30130100Minas GeraisBrazil

BACKGROUND Chronic hepatitis C(CHC)is associated with type 2 diabetes *** the pathogenesis remains to be elucidated,a growing evidence has suggested a role of pro-inflammatory immune *** serum concentrations of Interleukin 6(IL-6)have been associated with insulin resistance,type 2 diabetes mellitus as well as advanced forms of liver disease in chronic hepatitis C *** To investigate the frequency of IL-6-174G/C(rs1800795)single nucleotide polymorphism(SNP)in CHC patients and in healthy subjects of the same *** between type 2 diabetes mellitus(dependent variable)and demographic,clinical,nutritional,virological and,IL-6 genotyping data were also investigated in CHC *** Two hundred and forty-five patients with CHC and 179 healthy control subjects(blood donors)were prospectively *** 2 diabetes mellitus was diagnosed according to the criteria of the American Diabetes ***,biochemical,histological and radiological methods were used for the diagnosis of the liver ***-6 polymorphism was evaluated by Taqman SNP genotyping *** data were analysed by logistic regression *** Type 2 diabetes mellitus,blood hypertension and liver cirrhosis were observed in 20.8%(51/245),40.0%(98/245)and 38.4%(94/245)of the patients,*** frequency of the studied IL-6 SNP did not differ between the CHC patients and controls(P=0.81)and all alleles were in Hardy-Weinberg equilibrium(P=0.38).In the multivariate analysis,type 2 diabetes mellitus was inversely associated with GC and CC genotypes of IL-6-174(OR=0.42;95%CI=0.22-0.78;P=0.006)and positively associated with blood hypertension(OR=5.56;95%CI=2.79-11.09;P<0.001).CONCLUSION This study was the first to show that GC and CC genotypes of IL-6-174 SNP are associated with a decreased risk of type 2 diabetes mellitus in patients chronically infected with hepatitis C *** identification of potential inflammatory mediato

关键词： Chronic Hepatitis C Type 2 diabetes mellitus Interleukin 6-174G/Cgenepromoter single nucleotide polymorphism Blood hypertension Healthy control subjects

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Partial Nephrectomy as Treatment of an Atypical Metastasis from Prostate Cancer—A Case Report and Review of Literature

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Open Journal of Urology 2018年第1期8卷 8-16页

作者： Ulisses lopes Guerra Pereira Sobrinho André Luiz Lima diniz Rodrigo Galves Mesquita Martins Diogo Eugenio Abreu Da Silva Tomás Accioly De Souza José Anacleto Dutra De Resende Júnior Department of Urology Lagoa Federal Hospital Rio de Janeiro Brazil

Background: Prostate cancer is the second most common type of cancer in man and the second in cancer-specific deaths in this population in the world. Most of the causes of death related to prostate cancer are due to its distant metastases, with the most common sites being: skeleton, distant lymph nodes, liver and lung. Renal metastasis is rare, and studies suggest infiltration due to arterial microembolization of the tumor. A key point in this scenario is the clinical suspicion of differential diagnoses, to offer the patient an effective therapy in such a specific case. Aim: To report a case of a patient with prostate cancer undergoing partial nephrectomy whose histopathological report revealed a metastatic lesion of that primary site. Case Presentation: 74 years old man, referred in May 2015 due to high PSA level and lumbago. PSA 323.11 ng/dl, rectal examination cT3a;biopsy was performed and histopathological study reported bilateral prostate adenocarcinoma, Gleason’s score 8 (4 + 4). Patient’s staging showed multiple secondary implants on skeletal scintigraphy. Tomography revealed solid exophytic lesion in the lower pole of the right kidney (4.7 × 3.6 cm);prostate without cleavage planes with seminal vesicles and pelvic node enlargement. Hormone therapy was initiated, PSA levels dropped to 9.51 ng/dl and total testosterone Conclusion: Given the rarity of these cases, it is not possible to presume that nephrectomy enhances the survival rates. However, we observed that partial nephrectomy was a good choice for our patient, being the first case described in the literature. More reports should be available and studies with higher levels of evidence should be conducted to assist us in patient orientation and decision making.

关键词： Advanced Prostate Cancer Kidney Metastasis Partial Nephrectomy

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Prevalence of congenital cytomegalovirus infection in preterm,small for gestational age and low birth weight newborns:characteristics and cytokines profile

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World Journal of Pediatrics 2022年第7期18卷 498-504页

作者： Janaina Fortes Lino Llian Martins Oliveira diniz Debora Marques de Miranda Daniela Valadao Freitas Rosa Nathalia Gualberto Souza e Silva Eduardo de Souza Nicolau Larissa Goncalves Rezende Lais Silva Carvalho Marianna Fischer de Paula lopes Luisa Petri Correa Gabriela Mafra de Oliveira Flavia Miranda da Silva Alves Lorena Batista Pascoal Erika Lima Dolabella Teixeira da Costal Leni Marcia Anchieta Roberta Maia de Castro Romaneli Odete Valadares Maternity Hospital Belo HorizonteBrazil Pediatrics Department.Faculy of Medice of Federal University of Minas Gerais Belo Horizonte.Brazil Molecular Laboratory of the INCT-MM.Faculy of Medicine.Federal University of Minas Gerais-UFMG.Belo Horizonte Brazil Faculty of Medicine.Federal University of Minas Gerais-UFMG Belo Horizonte.Brazil Risoleta Tolentino.Neves University Hospital's Maternity Belo HorizonteBrazil Sofia Feldman Maternity Hospital Belo HorizonteBrazil Hospital das Clinicas Maternity-Federal University of Minas Gerais.Belo Horizonte Brazil Pediatrics Department of Federal University of Minas Gerais School Medicine.Minas Gerais.Av Professor Alfredo Balena 190.Belo Horizonte 30130-100Brazil

Cytomegalovirus is the most frequent agent of congenital viral infections,affecting approximately 0.2%-2.4%of live births in different countries[1-7].Universal screening for congenital cytomegalovirus infection in newborns is not a standard practice considering cost-benefit issues and viabil-ity of diagnostic ***,most newborns are asympto-matic and there is no effective treatment for this population[2-5].

关键词： weight birth characteristics

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