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Near-infrared leaf reflectance modeling of Annona emarginata seedlings for early detection of variations in nitrogen concentration

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Journal of Forestry Research 2023年第1期34卷 269-282页

作者： rafaela lancas gomes Marília Caixeta Sousa Felipe Girotto Campos Carmen Sílvia Fernandes Boaro José Raimundo de Souza Passos Gisela Ferreira Bioestatistic Plant BiologyParasitology and Zoology DepartmentBioscience Institute(IBB)Universidade Estadual Paulista(UNESP)BotucatuSao PauloBrazil

Nitrogen(N)monitoring is essential in nurseries to ensure the production of high-quality *** spectroscopy(NIRS)is an instantaneous,nondestructive method to monitor *** data such as NIRS can also provide the basis for developing a new vegetation spectral index(VSI).Here,we evaluated whether NIRS combined with statistical modeling can accurately detect early variations in N concentration in leaves of young plants of Annona emargiaata and developed a new VSI for this *** were grown in a hydroponics system with 0,2.75,5.5or 11 mM N for 45 *** we measured gas exchange,chlorophylla fluorescence,and pigments in leaves;analyzed complete leaf nutrients,and recorded spectral data for leaves at 966 to 1685 nm using *** a statistical learning approach,the dimensionality of the spectral data was reduced,then models were generated using two classes(N deficiency,N)or four classes(0,2.75,5.5,11 mM N).The best combination of techniques for dimensionality reduction and classification,respectively,was stepwise regression(PROC STEPDISC)and linear discriminant *** was possible to detect N deficiency in seedlings leaves with 100%precision,and the four N concentrations with93.55%accuracy before photosynthetic damage to the plant ***,NIRS combined with statistical modeling of multidimensional data is effective for detecting N variations in seedlings leaves of ***.

关键词： Mineral nutrition of plants Near-infrared spectroscopy Spectral vegetation index Digital signature Statistical learning Fluorescence of chlorophylla

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Distinguishing the Regional Atmospheric Controls on Precipitation Isotopic Variability in the Central-Southeast Portion of Brazil

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Advances in Atmospheric Sciences 2022年第10期39卷 1693-1708,I0018-I0020页

作者： Vinícius dos SANTOS Peter MARSHALL FLEMING Luís HENRIQUE MANCINI Stela DALVA SANTOS COTA Graziele Beatriz de LIMA rafaela RODRIGUES gomes Roberto Eduardo KIRCHHEIM Ricardo SANCHÉZ-MURILLO Didier GASTMANS São Paulo State University(UNESP) Environmental Studies Center.Av.24ABased1515-Bela Vista13.506-900Rio Claro(SP)Brazil Center for the Development of the Nuclear Technology(CDTN-CNEN) Av.Presidente Antônio Carlos6.627Campus da UFMG-Pampulha31270-90Belo Horizonte(MG)Brazil Universidade de Brasília-UnB Instituto de GeociênciasLaboratório de Isótopos Estáveis.Campus"Darcy Ribeiro"Asa Norte70.910-900Brasília(DF)Brasil Hydrology and Territorial Management Directory(DHT) The Geological Survey of Brazil(CPRM-SGB)São Paulo(SP)Brazil University of Texas at Arlington Earth and Environmental Sciences Department500 Yates StreetArlingtonTexas76019USA Stable Isotopes Research Group and Water Resources Management Laboratory Universidad NacionalHerediaCosta Rica

Precipitation isotope ratios(O and H)record the history of water phase transitions and fractionation processes during moisture transport and rainfall ***,we evaluated the isotopic composition of precipitation over the central-southeastern region of Brazil at different *** isotopic compositions were associated with classical effects(rainfall amount,seasonality,and continentality),demonstrating the importance of vapor recirculation processes and different regional atmospheric systems(South American Convergence Zone-SACZ and Cold Fronts-CF).While moisture recycling and regional atmospheric processes may also be observed on a daily timescale,classical effects such as the amount effect were not strongly correlated(δ^(18)O-precipitation rate r≤-0.37).Daily variability revealed specific climatic features,such asδ^(18)O depleted values(~-6‰to-8‰)during the wet season were associated with strong convective activity and large moisture *** isotopic analysis revealed the role of different moisture sources and transport *** ratios combined with d-excess explain how atmospheric recirculation processes interact with convective activity during rainfall formation *** findings provide a new understanding of rainfall sampling timescales and highlight the importance of water isotopes to decipher key hydrometeorological processes in a complex spatial and temporal context in central-southeastern Brazil.

关键词： precipitation stable water isotopes d-excess moisture source and transport southern Atlantic Ocean and Amazon

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Mercury in aquatic fauna contamination:A systematic review on its dynamics and potential health risks

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Journal of Environmental Sciences 2019年第10期31卷 205-218页

作者： Paloma de Almeida Rodrigues rafaela gomes Ferrari Luciano Neves dos Santos Carlos Adam Conte Junior Molecular and Analytical Laboratory Center Department of Food TechnologyFaculty of VeterinaryUniversidade Federal FluminenseNiteróiBrazil Chemistry Institute Food Science ProgramUniversidade Federal do Rio de JaneiroRio de JaneiroBrazil National Institute of Health Quality Control Fundacaeo Oswaldo CruzRio de JaneiroBrazil Laboratory of Theoretical and Applied Ichthyology Department of Ecology and Marine ResourcesUniversidade Federal do Estado do Rio de JaneiroRio de JaneiroBrazil

Mercury is an important pollutant,released into aquatic ecosystems both naturally and by anthropogenic *** element is transferred to aquatic organisms in different ways,causing potential health *** addition,mercury can be accumulated by humans,especially through the consumption of contaminated *** systematic review aims to present mercury pathways,the major routes through which this element reaches the aquatic environment and its transformations until becoming available to living animals,leading to bioaccumulation and biomagnification *** key biotic and abiotic factors affecting such processes,the impact of mercury on animal and human health and the issue of seafood consumption as a source of chronic mercury contamination are also addressed.A total of 101 articles were retrieved from a standardized search on three databases(PubMed,Emabse,and Web of Science),in addition to 28 other studies not found on these databases but considered fundamental to this review(totaling 129 articles).Both biotic and abiotic factors display fundamental importance in mediating mercurial dynamics,i.e.,muscle tropism,and salinity,***,mercurial contamination in aquatic environments affects animal health,especially the risk of extinction species and also on human health,with methylmercury the main mercury species responsible for acute and chronic symptomatology.

关键词： Mercury levels Seafood Methylmercury Public health Aquatic environment

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Pulmonary Tuberculosis Associated with Socioeconomic Vulnerability and Ineffective Support Network: Case Report

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Journal of Biosciences and Medicines 2023年第5期11卷 151-157页

作者： Michele Paula dos Santos Gabriela Di Lorenzo Garcia Scherer Carolina Hack Alves Italo Trois Maestri Carolina Boeira Soares Carlos Augusto Treviso Stella Duarte Pinto Ana Paula Wagner Steinmetz Laura gomes Boabaid de Barros Maria Eduarda Perobelli Buzetto Gabriel Lorentz Trein Guilherme Boeira Soares Aline Porto Brendda Pereira gomes Fernanda Bercht Merten Luane Pereira gomes Bárbara Adelmann de Lima rafaela Siviero Caron-Lienert Miriam Viviane Baron School of Medicine Pontifical Catholic University of Rio Grande do Sul (PUCRS) Porto Alegre Brazil Course of Medicine Lutheran University of Brazil (ULBRA) Canoas Brazil School of Health and Life Sciences Pontifical Catholic University of Rio Grande do Sul (PUCRS) Porto Alegre Brazil Interdisciplinary Institute of Science and Health Education Porto Alegre Brazil

Tuberculosis is one of the 10 leading causes of death in the world. Its symptoms include fever, malaise, weakness, weight loss, chest pain, cough, expectoration, shortness of breath and sepsis, which is its most frequent complication. Due to these symptoms, many patients with tuberculosis require admission to the Intensive Care Unit, where they usually are placed on mechanical ventilation. Tuberculosis is more prevalent in the population in situations of social and economic vulnerability. The main factors that interfere with adherence to treatment and the prognosis of these patients are the patient’s support network and their socioeconomic status. We present the following report of a long-time smoker patient, with chronic kidney disease and previous treatment for tuberculosis, who was brought to the emergency room due to lowering of the sensorium, and was immediately placed on mechanical ventilation. The tests suggested sepsis with a respiratory focus, so a search for Alcohol-Acid-Resistant Bacillus was carried out, with a positive result. The patient was transferred to the Intensive Care Unit due to tachycardia and acute respiratory failure. The patient was a smoker with a high tobacco load, chronic kidney disease, in addition to precarious economic, educational and self-care conditions. This report shows that patients with tuberculosis and its complications can be stabilized through known pharmacological treatment. However, the most effective measure to interrupt the transmission of the disease remains the early diagnosis and the provision of adequate treatment, with guidelines aimed at the cessation of harmful habits, such as smoking.

关键词： Smoking Kidney Disease Sepsis Respiratory Failure

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Sepsis after Administering an Intramuscular Iron Injection in the Arm of an Elder Patient with Multiple Comorbidities: A Case Report

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Journal of Biosciences and Medicines 2023年第5期11卷 158-165页

作者： Michele Paula dos Santos Taís Michele Werle Mariana Martins Dantas Santos Carolina Boeira Soares Carlos Augusto Treviso Italo Trois Maestri Stella Duarte Pinto Carolina Hack Alves Laura gomes Boabaid de Barros Maria Eduarda Perobelli Buzetto Guilherme Boeira Soares Gabriel Lorentz Trein Aline Porto Bárbara Adelmann de Lima Fernanda Bercht Merten Luane Pereira gomes rafaela Siviero Caron-Lienert Miriam Viviane Baron School of Medicine Pontifical Catholic University of Rio Grande do Sul (PUCRS) Porto Alegre Brazil Course of Medicine Lutheran University of Brazil (ULBRA) Canoas Brazil School of Medicine and Health Sciences Pontifical Catholic University of Rio Grande do Sul (PUCRS) Porto Alegre Brazil Interdisciplinary Institute of Science and Health Education Porto Alegre Brazil

Background: Skin reactions and musculoskeletal injuries caused by intramuscular injection are an increased risk for skin and soft tissue infections and may culminate in sepsis. Objective: To describe the history of an elderly patient who developed a lesion in her right arm after home administration of intramuscular iron injection in this arm and its outcome. Methods: This is a case report of a patient who participated in a study in an intensive care unit. Results: This study describes a 65-year-old patient with multiple comorbidities who was admitted to the hospital complaining of pain for two months and difficulty in moving her right shoulder associated with skin and soft tissue infections in her right arm after intramuscular iron administration by a relative for the treatment of multifactorial anemia and refractory melena. The patient worsened her general condition and was transferred to the intensive care unit. Despite the therapy instituted, the patient developed sepsis of cutaneous origin, multiple organ dysfunction, and death. Conclusions: The administration of intramuscular medication requires the participation of qualified professionals, such as nurses, mainly within the scope of the Brazilian public health system which commonly comprises patients in a context of vulnerability.

关键词： Critical Care Education Continuing Injectables Septicemia Soft Tissue Infections Suppuration

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Implementation of Quick HIV Tests in a Basic Health Unit in the Town of Santa Cruz-RN:Experiment Experienced by the PRO/PET-Saude Students

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Health 2015年第13期7卷 1782-1787页

作者： Ana rafaela Dantas dos Santos Ana Mayara gomes de Sousa Fernanda Diniz de Sá Adriana gomes Magalhaes Fábia Barbosa de Andrade Higo Luiz Amorim Belo Maia Faculty of Health Sciences of Trairi(FACISA) Federal University of Rio Grande do Norte(UFRN)Santa CruzBrazil

This article is about a report of the experiment of PRO/PET-Saúde students in the anti-HIV tests implementation in the town of Santa Cruz-RN. The goal is to report the importance and discuss the aspects of the moment experienced above inside the basic health unit in the town of Santa Cruz-RN. The experience occurred in the months from February to March 2014 and they were conducted within 8 hours per week. This way, it is observed that the period lived made possible great reflection about the making of the examination in the pre-natal appointment routines, cataloguing challenges which come up during their adequacy within the services. Lastly, the action developed aimed for the aggregation of an active learning process in the search for a critical sense in the experienced practice, allowing and prioritizing the basic health unit as a scope of reference and counter-reference in the public health service, increasing concrete answers and the needs of the population, guaranteeing an adequate service delivery and strengthening the SUS more and more. The insertion of students through the PRO/PET-Saúde-Rede Cegonha enables a reorientation of professional formation, from the integration between service and teaching.

关键词： Pregnant Student Teaching-Service HIV Tests Vertical Transmissions

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Portal Gas Embolism with Acute Liver Ischemia： Case Report

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Journal of Pharmacy and Pharmacology 2017年第12期5卷 913-916页

作者： Gabriela Delatore gomes Bruno Hafemann Moser rafaela Orikba Ricardo Panico Rizzo Luiz Lucas Baptism da Slva Augusto Fey Faculty of Medicine UNIDAVI Rio do Sul 89160-93 2 Brazil Department of Health Sciences Pontifical Catholic University Curitiba 80215-901 Brazil

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Occurrence of Antimicrobials in River Water Samples from Rural Region of the State of Rio de Janeiro, Brazil

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Journal of Environmental Protection 2016年第2期7卷 230-241页

作者： Mychelle Alves Monteiro Bernardete Ferraz Spisso Julia Rodrigues Martins Pastor dos Santos rafaela Pinto da Costa Rosana gomes Ferreira Mararlene Ulberg Pereira Talita da Silva Miranda Bárbara Rodrigues Geraldino de Andrade Luiz Antonio d’Avila Chemistry School/Federal University of Rio de Janeiro (EQ/UFRJ) Rio de Janeiro Brazil National Institute for Quality Control in Health/Oswaldo Cruz Foundation (INCQS/FIOCRUZ) Rio de Janeiro Brazil University Centre of Barra Mansa (UBM) Barra Mansa Brazil National of Institute of Cancer (INCA) Rio de Janeiro Brazil

The occurrence of antimicrobials in the aquatic environment and drinking water has raised the question of their impact on the environment and public health. Animal production is one of the most expressive activities of Brazilian agribusiness. In order to ensure the productivity and competitiveness of the sector, the use of drugs for therapeutic and prophylactic purposes is a common practice. Due to the continuous release of antimicrobials into the environment, the aim of this study was to compare the frequency of detection of tetracyclines and sulfonamides in surface water collected from rural areas in Lidice District of Rio Claro, in the State of Rio de Janeiro, Brazil. An investigative study was conducted with 24 river water samples analyzed by high-performance liquid chromatography coupled to tandem mass spectrometry and the aim of this study was to determine residues of sulfonamides and tetracyclines based on the USEPA method 1694. The results indicated the presence of sulfamethoxazole and oxytetracycline concentrations at the ng·L-1 level. The applied method showed overall good performance with recoveries above 57%, method detection limits ≤ 7.17 ng·L-1, method quantification limits ≤ 23.90 ng·L-1 and good linearity.

关键词： Antimicrobial LC-MS/MS Surface Water Sulfonamides Tetracyclines

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Three-dimensional models of antigens with serodiagnostic potential for leprosy:An in silico study

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World Journal of Clinical Infectious Diseases 2023年第1期13卷 1-10页

作者： Bianca Luiza Melo de Assis rafaela Viana Vieira Ian Theodoro Rudenco gomes Palma Matheus Bertolini Coutinho Juliana de Moura Gabrielle Caroline Peiter Kádima Nayara Teixeira Campus Toledo Universidade Federal do ParanáToledo 85.919-899ParanáBrazil Departamento de Patologia Básica Universidade Federal do Paraná-Setor de Ciências BiológicasCuritiba 81.531-980ParanáBrazil Programa Multicêntrico de Pós-graduação em Bioquímica e Biologia Molecular-Setor Palotina Universidade Federal do ParanáPalotina 85.950-000ParanáBrazil

BACKGROUND Leprosy is a disease caused by Mycobacterium leprae(***),an intracellular pathogen that has tropism and affects skin and nervous system *** disease has two forms of presentation:Paucibacillary and multibacillary,with different clinical and immunological *** what occurs in the multibacillary form,the diagnostic tests for the paucibacillary form are nonspecific and not very sensitive,allowing the existence of infected individuals without treatment,which contributes to the spread of the pathogen in the *** mitigate this contamination,more sensitive diagnostic tests capable of detecting paucibacillary patients are *** To predict the three-dimensional structure models of *** antigens with serodiagnostic potential for *** In this in silico study,satisfactory templates were selected in the Protein Data Bank(PDB)using Basic Local Alignment Search Tool to predict the structural templates of ML2038,ML0286,ML0050,and 85B antigens by comparative *** templates were selected according to general criteria such as sequence identity,coverage,X-ray resolution,Global Model Quality Estimate value and phylogenetic relationship;Clustal X 2.1 software was used in this *** modeling was completed using the software Modeller *** of the models was made using ViewerLite 4.2 and PyMol software,and analysis of the quality of the predicted models was performed using the QMEAN score and ***,the three-dimensional moels were validated using the MolProbity and Verify 3D *** The three-dimensional structure models of ML2038,ML0286,ML0050,and 85B antigens of *** were predicted using the templates PDB:3UOI(90.51%identity),PDB:3EKL(87.46%identity),PDB:3FAV(40.00%identity),and PDB:1F0N(85.21%identity),*** QMEAN and Z-score values indicated the good quality of the structure *** data refer to the monomeric units of antigens,since some of these a

关键词： Antigens Leprosy diagnosis Mycobacterium leprae Molecular modelling Serological test In silico study

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Chemical Characterization and in Vitro Antitumor Activity of the Essential Oils from the Leaves and Flowers of Callistemon viminalis

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American Journal of Plant Sciences 2015年第16期6卷 2664-2671页

作者： Christiane Maria de Oliveira Maria das Gracas Cardoso Marisa Ionta Marisi gomes Soares Juliana de Andrade Santiago Guilherme álvaro Ferreira da Silva Maria Luisa Teixeira Danúbia Aparecida de Carvalho Selvati Rezende rafaela Vieira de Souza Luana Isac Soares David Lee Nelson Marcos Schleiden Sousa Carvalho Department of Chemistry Federal University of LavrasLavrasBrazil Institute of Biomedical Sciences Federal University of AlfenasAlfenasBrazil Department of Chemistry Federal University of AlfenasAlfenasBrazil Federal University of Vales de Jequitinhonha e Mucuri DiamantinaBrazil Department of Agriculture Federal University of LavrasLavrasBrazil

The objective of this study was to characterize and verify the in vitro antitumor activity of essential oils (EOs) extracted from the leaves and flowers of Callistemon viminalis. The EOs were extracted by hydrodistillation using a modified Clevenger apparatus. The identification and quantification of constituents were performed on a gas chromatograph coupled to a mass spectrometer and a gas chromatograph with a flame ionization detector. The antitumoral activity was evaluated by a colorimetric assay (MTS) using different cell lines derived from human tumors (breast, lung, glioblastoma, and melanoma). The major constituents of the EOs of leaves and flowers were similar, only quantitative differences being observed. The compounds 1,8-cineole, α-pinene and α-terpineol were found in concentrations of 50.4%, 25.8% and 8.7% in the EOs obtained from the leaves and 48.8%, 24.5% and 3.9% in the EOs obtained from the flowers, respectively. The cytotoxic activity of the EOs was observed only in melanoma cultures (HT144). Cultures treated for 48 h with EOs from leaves and flowers (200 μg·mL-1) reduced the viability by 40% and 25%, respectively. Thus, the antiproliferative activity of the EO from leaves was more pronounced than the EO from flowers in cells derived from melanoma.

关键词： Cancer Volatile Oil Escova-de-Garrafa(Bottle Brush)

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