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国际纺织导报 2019年第11期47卷 16-18,20页

作者： V.Tenner M.I.Popzyk R.Z.Dangel T.Gries R.Van Olmen 刘书惠(译) 孟粉叶(校) 亚琛工业大学纺织技术研究所(德国) Centexbel公司(比利时) 不详

介绍了Spun EcoYarn(SPEY)项目的研究现状。该项目旨在通过方法学分析与工艺分析,研究聚乳酸(PLA)短纤纱在家用或产业用纺织品领域的应用。工艺过程分析的目的是避免PLA在环锭纺过程中出现降解现象。目前,项目已成功制备出100%PLA环锭... 详细信息

介绍了Spun EcoYarn(SPEY)项目的研究现状。该项目旨在通过方法学分析与工艺分析,研究聚乳酸(PLA)短纤纱在家用或产业用纺织品领域的应用。工艺过程分析的目的是避免PLA在环锭纺过程中出现降解现象。目前,项目已成功制备出100%PLA环锭纱。当生产速度为23 m/min、纱线捻度为100 T/m时,可纺制25 tex的PLA纱,且纺纱工艺稳定、无断头。概述了采用PLA长丝进行环锭纺的试验结果,提出了稳定生产100%PLA环锭纱的优化工艺参数。

关键词：聚乳酸(PLA) 短纤纱捻度稳定生产

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伏令夏橙在21种砧木上七年后的初步结果

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西南园艺 1985年第1期 40-44页

作者： D. J. Hutchinson W. f. Bistine 文泽富

我们在1974—1981年,对伏令夏橙(***(L.)Osb.)嫁接在21种砧木上树冠体积、产量及品质进行了研究。研究表明,伏令夏橙嫁接在卡里佐枳橙、尤马枳橙(***×***(L.)raf)、粗柠檬807和瓦尔卡默柠檬(***(L.)Burmf.)等砧木上,其单株产量和单株... 详细信息

我们在1974—1981年,对伏令夏橙(***(L.)Osb.)嫁接在21种砧木上树冠体积、产量及品质进行了研究。研究表明,伏令夏橙嫁接在卡里佐枳橙、尤马枳橙(***×***(L.)raf)、粗柠檬807和瓦尔卡默柠檬(***(L.)Burmf.)等砧木上,其单株产量和单株固形物含量最高;嫁接在卡里佐枳橙、尤马椒橙、粗柠檬、粗柠檬807、酸橙2号(*** L.)、酸橙839和瓦尔卡默柠檬等砧木上的树冠体积最大;嫁接在阿根廷帜、波默罗伊枳(***)、诺顿枳橙(***×***)、酸橙895等砧木上的果实总可溶性固形物含量最高。

关键词：砧木树冠体积瓦尔台木嫁接材料树冠容积伏令夏橙粗柠檬

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Urothelial bladder cancer progression: lessons learned from the bench

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Journal of Cancer Metastasis and Treatment 2015年第1期1卷 57-66页

作者： Julieta P.Afonso Rui freitas francisco Lobo António Morais Jorge Oliveira Teresina Amaro Rui M.Reis fátima M.Baltazar Adhemar Longatto-filho Lúcio L.Santos Life and Health Sciences Research Institute(ICVS) School of Health SciencesUniversity of MinhoCampus de Gualtar4710-057 BragaPortugal ICVS/3B’s-PT Government Associate Laboratory 4806-909 Braga/GuimarãesPortugal Department of Urology Portuguese Institute of OncologyRua Dr.António Bernardino de Almeida4200-072 PortoPortugal Experimental Pathology and Therapeutics Research Center Portuguese Institute of OncologyRua Dr.António Bernardino de Almeida4200-072 PortoPortugal Molecular Oncology Research Center Barretos Cancer HospitalCEP 14784-400 BarretosSão PauloBrazil Laboratory of Medical Investigation(LIM 14) Faculty of MedicineSão Paulo State UniversityCEP 01246-000São PauloBrazil Department of Surgical Oncology Portuguese Institute of OncologyRua Dr.António Bernardino de Almeida4200-072 PortoPortugal faculty of Health Sciences University Fernando PessoaRua Carlos da Maia4200-150 PortoPortugal.

Urothelial bladder carcinoma(UBC)is an intricate malignancy with a variable natural history and clinical *** developments in diagnosis/prognosis refi nement and treatment modalities,the recurrence rate is high,and progression from non-muscle to muscle invasive UBC commonly leads to ***,patients with muscle-invasive or extra-vesical disease often fail the standard chemotherapy treatment,and overall survival rates are ***,UBC remains a challenge in the oncology fi eld,representing an ideal candidate for research on biomarkers that could identify patients at increased risk of recurrence,progression,and ***,progress toward personalized medicine has been hampered by the unique genetic complexity of *** genome-wide expression and sequencing studies have brought new insights into its molecular features,pathogenesis and clinical diversity,revealing a landscape where classical pathology is intersected by the novel and heterogeneous molecular ***,it seems plausible to postulate that only an integrated signature of prognostic/predictive biomarkers inherent in different cancer hallmarks will reach clinical *** this review,we have summarized ours and others’research into novel putative biomarkers of progression and chemoresistance that encompass several hallmarks of cancer:tumor neovascularization,invasion and metastasis,and energy metabolism reprogramming of the tumor microenvironment.

关键词： CD147 lymphovascular invasion mammalian target of rapamycin monocarboxylate transporters progression raf kinase inhibitor protein scoring system urothelial bladder cancer

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Na+ - Ca2+ Exchanger Gene Products. NCX1, NCX2 and NCX3, as Putative Targets for Neuroprotection in Brain Ischemia

Na+ - Ca2+ Exchanger Gene Products. NCX1, NCX2 and NCX3, as ...

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第十五届国际药理学大会

作者： Annunziato L Pignataro G Sirabella R Saggese M Cuomo O Gala R Sec-ondo A Viggiano D Boscia f Molinaro P Valsecchi V Tortiglione A Di Renzo Gf Division of Pharmacology Depof NeuroscienceSchool of MedicineUniv.of Naples Federico IIItaly Division of Pharmacology Depof NeuroscienceSchool of MedicineUniv.of Naples Federico IIItaly Division of Pharmacology Depof NeuroscienceSchool of MedicineUniv.of Naples Federico IIItaly Division of Pharmacology Depof NeuroscienceSchool of MedicineUniv.of Naples Federico IIItaly Division of Pharmacology Depof NeuroscienceSchool of MedicineUniv.of Naples Federico IIItaly Division of Pharmacology Depof NeuroscienceSchool of MedicineUniv.of Naples Federico IIItaly Division of Pharmacology Depof NeuroscienceSchool of MedicineUniv.of Naples Federico IIItaly Division of Pharmacology Depof NeuroscienceSchool of MedicineUniv.of Naples Federico IIItaly Division of Pharmacology Depof NeuroscienceSchool of MedicineUniv.of Naples Federico IIItaly Division of Pharmacology Depof NeuroscienceSchool of MedicineUniv.of Naples Federico IIItaly Division of Pharmacology Depof NeuroscienceSchool of MedicineUniv.of Naples Federico IIItaly Division of Pharmacology Depof NeuroscienceSchool of MedicineUniv.of Naples Federico IIItaly Division of Pharmacology Depof NeuroscienceSchool of MedicineUniv.of Naples Federico IIItaly

＜正＞ NCX is a neuronal plasmamembrane antiporter, which, by coupling Ca2 + and Na+ fluxes, plays a relevant role in brain ischemia. In the CNS there are 3 different NCX gene products NCX1, NCX2, and NCX3. NCX transcript and protein expression is differently regulated after ischemia in the focal region and in the peri-infarct area. The pharmacological inhibition of NCX activity is detrimental in the development of ischemic damage. NCX knocking out through antisense strategy showed that NCX1 and NCX3 play a major role in NCX neuroprotective action. Accordingly. NCX3 transfected BHK cells are less vulnerable to chemical hypoxia. Interestingly, the expression of NCX1 and NCX3 is up-regulated by NGf in PC12 cells. This modulation occurs via two pathways activated by tyro-sine kinase receptors. Ras-raf-Mek-MAPK pathway and PI-3K/AKT pathway. In fact, the Mek inhibitor PD 98059 or the PI-3K inhibitor LY 294002 prevented this up-regulation. whereas AKT-1 PC12 cell positive mutants showed an increase in NCX1 and NCX3 expression. These data demonstrate that NCX products display a differential expression in the development of ischemic damage and can be selectively upregulated through NGf-mediated trasductional pathways

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Construction of a food-Grade Expression Vector Based on pMG36e by Using an α-Galactosidase Gene as a Selectable Marker

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Journal of Integrative Agriculture 2014年第8期13卷 1802-1808页

作者： GU Xin-xi TAN Jian-xin TIAN Hong-tao ZHANG Yu-lan LUO Yun-bo GUO Xing-hua College of food Science and Technology Agricultural University of Hebei College of f ood Science and Nutritional Engineering China Agricultural University Institute of Microbiology Chinese Academy of Sciences

Construction of a food-grade expression vector for application to lactic acid bacteria（LAB） is of importance for dairy fermentation system. An α-galactosidase（aga） gene encoding an enzyme degrading melibiose was amplified by PCR from the plasmid p raf800 of Lactococcus lactis NZ9000. The aga gene was introduced into pMG36 e to substitute the p rimary antibiotic selectable marker of pMG36 e, resulting in construction of a new food-grade expression vector pMG36-aga. To testify the expression efficiency of exogenous gene in pMG36-aga, a 1.5 kb long α-amylase（amy） gene from Ba cillus li cheniformis was cloned by PCR and introduced into the plasmid pMG36-aga. The resultant plasimd pMG36-aga-amy was transformed into L. lactis ML23 by electroporation. The positive clones were selected with the medium containing melibiose as the sole carbon source. Th e selection efficiency of aga was 8.71×103 CfU with a standard deviation of 9.1×102 CfU ？g-1 DNA of pMG36-aga. furthermore, the SDS-PAGE analysis showed that the pMG36-aga-amy expressed a 56.4 kDa protein which was the same as the putati ve molecular weight of α-amylase. The starch plate assay also indicated that L. lactis ML23 displayed high activity of α-amylase by expressing of amy gene of pMG36-aga-amy.

关键词： food-grade expression vector Lactococcus lactis α-galactosidase gene amylase gene pMG36e

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