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ATR–FTIR Spectroscopy Combined with the Invitro Antioxidant Activity and Chromaticity for Rapid Discrimination of Fig(Ficus carica L.)Cultivars

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Journal of Analysis and Testing 2021年第3期5卷 270-285页

作者： Lahcen Hssaini rachida ouaabou Rachid Razouk Jamal Charafi Hafi da Hanine Karim Houmanat Said Ennahli Rachid Lahlali National Institute for Agricultural Research(INRA) BO578MeknesMorocco Faculty of Science Semlalia Cadi Ayyad UniversityBO 2390MarrakeshMorocco Laboratory of Bioprocess and Bio-Interfaces Faculty of Science and TechnicsBO 523Beni-MellalMorocco National School of Agriculture(ENA) BO S/40MeknesMorocco

Vibrational spectroscopy analysis of full-ripened fig fruits(Ficus carica L.)was acquired using Fourier-transformed infrared(FTIR)spectroscopy by attenuated total reflectance(ATR).The objective of this study was to investigate accurate discrimination of 25 fig cultivars using chemometric analysis of both ATR-FTIR fingerprinting and in-vitro antioxidant activity along with chromatic coordinates ***-vitro antioxidant activity was assessed through three methods and displayed statistically significant differences across cultivars and between the fruit parts,with promising antioxidant *** spectra were measured separately on both peels and pulp to better know which fruit part provides high discrimination throughput between *** results showed vibration intensities of five fingerprint regions displaying statically significant *** highest vibration intensity occurred in the region of 1175-940 cm^(-1)including the phenols *** components analysis based on integrated intensities corresponding to fingerprint regions,revealed two main groups in pulp samples,while five groups in peels *** pulp extracts of’Nabout’showed a typical vibration in 3000-2800 cm^(-1)and 1775-1725 cm^(-1)regions and,therefore was classified as single subset in cultivars *** cultivar had a lightcolored fig and exhibited the highest antioxidant potency for all essays particularly in peel *** biochemical assessment and FTIR fingerprinting data were involved in PCA analysis,and that displayed some dissimilarities in the classification patterns,given the fact that antioxidant activities and chromaticity together could not totally explain the classification based on FTIR fingerprinting between peel and *** of FTIR-ATR spectroscopy to discriminate fig cultivars seems to be a rapid,accurate and cost-effective alternative to laborious measurement techniques,in fig quality screening and preselection,such as chromatog

关键词： Chemometrics FTIR-ATR Ficus carica L. Half maximal inhibitory concentration Multivariate analysis

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