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Use of reducing agents for the aerobic growth of Campylobacter jejuni

Asian Pacific Journal of Tropical Medicine 2010年第3期3卷 235-236页

作者： rabia sultana siddiqui Siti Asma H School of Dental Sciences Universiti Sains Malaysia16150 Kubang KerianKelantanMalaysia School of Medical Sciences Universiti Sains Malaysia16150 Kubang KerianKelantanMalaysia

Objective:To produce a technique for the growth of Campylobacter jejuni in aerobic condition Methods:Different combinations of reducing agents were tested in brucella broth and the growth turbidity was compared with tubes containing normal broth *** environment was also provided in a petri plate seeded with Campylobacter culture by pouring 3 different concentrations（10%,5%and 2.5%） of five reducing agents along with bacto-agar in the lid which was used to cover and seal the culture *** reducing agents were also added in broth in concentration of 0.25 mg/mL of each with different ***:In lid agar technique,Campylobacter jejuni growth appeared in all three concentrations of reducing agents, that is 10%,5%and 2.5%after 24 hours of incubation but the best results were observed in 10% *** colonial and morphological characters were not affected when the organisms were grown by this ***:It was found that reducing agents enhance the growth of ***/*** combination of FeSO4,Na2CO3 with H3BO3 worked as ideal mixture for the aerobic growth of *** technique is more economical as compared to commercially available media in the market and can be used for the oral facultative and microaerophilic bacterial growth in laboratory.

关键词： Camylobacter jejuni Microaerophilic Reducing agents Lid agar technique

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RP-HPLC Method for the Simultaneous Determination of Lisinopril and NSAIDs in API, Pharmaceutical Formulations and Human Serum

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American Journal of Analytical Chemistry 2012年第2期3卷 147-152页

作者： Najma sultana M. Saeed Arayne Rubina siddiqui Safila Naveed Fatima Jinnah Medical and Dental College Karachi Pakistan Jinnah University for Women Karachi Pakistan United Biotechnologies Karachi Pakistan

High performance liquid chromatographic method was developed valdated and applied for the simultaneous determi- nation of lisinopril and NSAIDs in bulk, pharmaceuticals formulations and human serum. A Purospher star C18 (5 μm, 25 × 0.46 cm) column was used with mobile phase consisting of methanol: water: acetonitrile (80:17.5:2.5 v/v, pH 3.0) and quantitative evaluation was performed at 225 nm with a flow rate of 1.0 mL?min–1. The retention time of lisinopril was 2.2 min while naproxen, flurbiprofen, diclofenac sodium and mefenamic acid were found to be 4.0, 4.5, 5.0 and 6.7 min respectively. Suitability of this method for the quantitative determination of the drugs was proved by validation in accordance with the requirements laid down by International Conference on Harmonization (ICH) guidelines. The method is selective, precise, accurate and can be used for analysis of pharmaceutical preparations in quality control and clinical laboratories.

关键词： Lisinopril NSAIDs Method Validation HPLC Determination

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RP-HPLC Method for the Simultaneous Determination of Lisinopril and NSAIDs in API,Pharmaceutical Formulations and Human Serum

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American Journal of Analytical Chemistry（美国分析化学） 2012年第2期

作者： Najma sultana M. Saeed Arayne Rubina siddiqui Safila Naveed

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