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Partial Fusion (F) Gene Analysis of Newcastle Disease Virus Detected in Pakistan during 2021-2022

Journal of Biosciences and Medicines 2024年第5期12卷 256-275页

作者： Muhammad Danish Mehmood Huma Anwar Ul-Haq Rauf Khalid Yasir Amin Muhammad Usman Ghani Muhammad Ismail rabia habib Fareeha Arshed Abdul Rasheed Shaukat Institute of Molecular Biology & Biotechnology University of Lahore Lahore Pakistan Research & Development Division Ottoman Pharma Lahore Pakistan Quality Assurance Department Ottoman Pharma Lahore Pakistan Veterinary Research and Disease Investigation Center Abbottabad Pakistan Quality Control Department Ottoman Pharma Immuno Lahore Pakistan Production Department Ottoman Pharma Lahore Pakistan Poultry Production Department Sabir&#8217s Poultry Pvt. Ltd. Sabroso Pakistan

Newcastle disease (ND) virus is a leading threat to commercial and domestic poultry in Pakistan. The virus infects and constitutes irreversible impairment to the nervous system, damages the respiratory system, and marks severe gastrointestinal lesions leading to heavy mortality in short-living birds and substantial losses in layers and breeders. The continuous emergence and evolution of the virus made it inclined to evade the humoral response and indirectly the circumvention of artificial active immunization. Newcastle disease is caused by the orthoavula genus of the paramyxoviridae family and has shown high genetic diversity even in their genotypes while information regarding enzootic trends of the virus is scanty in Pakistan. A total of 40 tracheal samples of NDV were collected from different commercial broiler farms and 11 isolates of NDV were identified. In the current study, we determined the genetic diversity of the Newcastle disease virus based on the partial sequencing of the fusion protein gene available in the NCBI database. Genetic analysis showed that seven isolates belonged to class I genotype VII and four belonged to class II genotype II. Interestingly, two isolates had epidemiological connections with vaccine-like class II genotype II. Our findings, concerning the recent outbreaks of class I genotype VII and class II genotype II of NDV in vaccinated commercial flocks, suggest possible potential partial mutations in the fusion protein gene. Genetic diversity and formation of the new cleavage site in an important neutralizing protein of wild strain are linked with the potency of artificial active immunization and a major cause of vaccine failure.

关键词： Newcastle Disease Virus Haemagglutination Inhibition Polymerase Chain Reaction Phylogenetic Tree Mutation Analysis

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Automated Grading of Breast Cancer Histopathology Images Using Multilayered Autoencoder

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Computers, Materials & Continua 2022年第5期71卷 3407-3423页

作者： Shakra Mehak M.Usman Ashraf rabia Zafar Ahmed M.Alghamdi Ahmed S.Alfakeeh Fawaz Alassery habib Hamam Muhammad Shafiq Knowledge Unit of System and Technology University of Management&TechnologySialkot Campus51310Pakistan Department of Computer Science University of Management and TechnologySialkot Campus51310Pakistan Faculty of Computing and Technology University of Engineering&TechnologyNarowal51610Pakistan College of Computer Science and Engineering University of Jeddah21493Saudi Arabia Faculty of Computing and Information Technology King Abdulaziz UniversityJeddah21589Saudi Arabia Department of Computer Engineering College of Computers and Information TechnologyTaif UniversityTaifSaudi Arabia Faculty of Engineering Moncton UniversityNBE1A3E9Canada Department of Information and Communication Engineering Yeungnam UniversityGyeongsan38541Korea

Breast cancer(BC)is the most widely recognized cancer in women *** 2018,627,000 women had died of breast cancer(World Health Organization Report 2018).To diagnose BC,the evaluation of tumours is achieved by analysis of histological *** present,the Nottingham Bloom Richardson framework is the least expensive approach used to grade BC *** contemplate three elements,*** count,*** formation,and *** atypia,which is a laborious process that witness’s variations in expert’s ***,some algorithms have been proposed for the detection of mitotic cells,but nuclear atypia in breast cancer histopathology has not received much *** atypia analysis is performed not only to grade BC but also to provide critical information in the discrimination of normal breast,non-invasive breast(usual ductal hyperplasia,atypical ductal hyperplasia)and pre-invasive breast(ductal carcinoma in situ)and invasive breast *** proposed a deep-stacked multi-layer autoencoder ensemble with a softmax layer for the feature extraction and classification *** classification results show the value of the multilayer autoencoder model in the evaluation of nuclear *** proposed method has indicated promising results,making them more fit in breast cancer grading.

关键词： Breast cancer nuclear atypia autoencoder

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