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tRnA^(Glu)-derived fragments from embryonic extracellular vesicles modulate bovine embryo hatching

Journal of Animal Science and Biotechnology 2024年第4期15卷 1559-1566页

作者： Yuan Fan Krishna Chaitanya Pavani Katrien Smits Ann Van soom Luc Peelman Department of Veterinary and Biosciences Faculty of Veterinary MedicineGhent UniversityHeidestraat 199820 MerelbekeBelgium Department of Internal Medicine Reproduction and Population MedicineFaculty of Veterinary MedicineGhent UniversitySalisburylaan 1339820 MerelbekeBelgium Department for Reproductive Medicine Ghent University HospitalCorneel Heymanslaan 109000 GhentBelgium

Transfer RnA-derived small RnAs(tsRnAs)have been shown to be involved in early embryo development and repression of endogenous retroelements in embryos and stem ***,it is unknown whether tsRnAs also regulate embryo *** this study,we mined the sequencing data of a previous experiment in which we demonstrated that the microRnA(miRnA)cargo of preimplantation embryonic extracellular vesicles(EVs)influences embryo *** thus profiled the tsRnA cargo of EVs secreted by blastocysts and *** majority of tsRnAs was identified as tRnA halves originating from the 5'ends of *** the 148 differentially expressed tsRnAs,the 19 nt tRnA fragment(tRF)tDR-14:32-Glu-CTC-1 was found to be significantly up-regulated in EVs derived from ***-qPCR assays confirmed its significant up-regulation in non-blastocyst embryos and their conditioned medium compared to the blastocyst group(Pnhibition of tDR-14:32-Glu-CTC-1 by supplementing antagomirs to the conditioned medium improved embryo hatching(Pnscriptomic analysis of embryos treated with tDR-14:32-Glu-CTC-1 antagomirs further showed differential expression of genes that are associated with embryo hatching and *** summary,tDR-14:32-Glu-CTC-1 is up-regulated in non-blastocyst embryos and their secretions,and inhibition of tDR-14:32-Glu-CTC-1 promotes embryo hatching,while influencing embryo implantation-related genes and *** results indicate that embryonic EVs containing specific tRFs may regulate preimplantation embryo development.

关键词： Embryo Extracellular vesicles Hatching tRnA fragments ts RnAs

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Leaf chlorophyll and nitrogen dynamics and their relationship to lowland rice yield for site-specific paddy management

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Information Processing in Agriculture 2017年第4期4卷 259-268页

作者： Asa Gholizadeh Mohammadmehdi Saberioon Lubos Boruvka Aimrun Wayayok Mohd Amin Mohd soom Department of Soil Science and Soil Protection Faculty of AgrobiologyFood and Natural ResourcesCzech University of Life Sciences PraguePragueCzech Republic Laboratory of Signal and Image Processing Institute of Complex SystemsSouth Bohemia Research Centre of Aquaculture and Biodiversity of HydrocenosesFaculty of Fisheries and Protection of WatersUniversity of South Bohemia in Ceske BudejoviceNove HradyCzech Republic Department of Biological and Agricultural Engineering Faculty of EngineeringUniversity Putra MalaysiaSelangorMalaysia Faculty of Sustainable Agriculture University Malaysia SabahSandakanMalaysia

The optimum rate and application timing of nitrogen(n)fertilizer are crucial in achieving a high yield in rice cultivation;however,conventional laboratory testing of plant nutrients is time-consuming and *** develop a site-specific spatial variable rate application method to overcome the limitations of traditional techniques,especially in fields under a double-cropping system,this study focused on the relationship between Soil Plant Analysis Development(SPAD)chlorophyll meter readings and n content in leaves during different growth stages to introduce the most suitable stage for assessment of crop n and prediction of rice *** SPAD readings and leaf n content were measured on the uppermost fully expanded leaf at panicle formation and booting *** yield was also measured at the end of the *** analysis of variance,variogram,and kriging were calculated to determine the variability of attributes and their relationship,and finally,variability maps were *** linear relationships were observed between attributes,with the same trends in different sampling dates;however,accuracy of semivariance estimation reduces with the growth *** of the study also implied that there was a better relationship between rice leaf n content(R^2=0.93),as well as yield(R2=0.81),with SPAD readings at the panicle formation ***,the SPAD-based evaluation of n status and prediction of rice yield is more reliable on this stage rather than at the booting *** study proved that the application of SPAD chlorophyll meter paves the way for real-time paddy n management and grain yield *** can be reliably exploited in precision agriculture of paddy fields under double-cropping cultivation to understand and control spatial variations.

关键词： Spatial variability non-invasive measurement Precision farming Decision support

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Hydrogenation of Alkenes with naBH₄, CH₃Co₂H, Pd/C in the Presence of o- and n-Benzyl Functions

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International Journal of organic Chemistry 2016年第1期6卷 1-11页

作者： nuha Al soom Thies Thiemann Department of Chemistry United Arab Emirates University Al Ain Abu Dhabi United Arab Emirates

naBH4, CH3Co2H, Pd/C has been described as an effective reagent system to hydrogenate alkenes. Here, we show that the hydrogenation occurs chemoselectively, making it possible to hydrogenate alkenes under Pd/C catalys... 详细信息

关键词： Alkene Hydrogenation Benzyl Ether Benzyl Ester n-Benzyl Group

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