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胆管癌中m7G甲基化调节因子相关预后风险模型的构建及临床意义

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生物医学工程与临床 2024年第2期28卷 254-264页

作者：王卓王敏刘莹吕丹丹屈园园谌慧君洪流周威空军军医大学第一附属医院消化病医院陕西西安710032

目的表观遗传重编程在胆管癌(CCA)进展中起关键作用,m7G甲基化修饰是转录后调控中最常见的碱基修饰形式之一,其通过调节肿瘤相关基因的表达在增殖、侵袭和转移等多种肿瘤相关的生物学活动中发挥着巨大作用。实验研究是探索m7G甲基化调... 详细信息

目的表观遗传重编程在胆管癌(CCA)进展中起关键作用,m7G甲基化修饰是转录后调控中最常见的碱基修饰形式之一,其通过调节肿瘤相关基因的表达在增殖、侵袭和转移等多种肿瘤相关的生物学活动中发挥着巨大作用。实验研究是探索m7G甲基化调节因子构建的相关修饰模式及其对CCA临床预后及治疗效果的评估。方法分析基于癌症基因组图谱(TCGA)和基因表达综合数据库(GEO)中27个m7G甲基化调节因子[Argonaute2蛋白(AGO2),细胞质脆性X智力迟钝蛋白1(FMR1)相关蛋白1(CYFIP1),脱帽mRNA2(DCP2)、清道夫脱帽酶(DCPS),真核翻译起始因子3(EIF3)亚基D(EIF3D),真核翻译起始因子4A1(EIF4A1),真核翻译起始因子4E(EIF4E),真核翻译起始因子4E家族成员1B(EIF4E1B),真核翻译起始因子4E家族成员2(EIF4E2),真核翻译起始因子4E家族成员3(EIF4E3),真核翻译起始因子4伽玛3(EIF4G3),宝石核细胞器相关蛋白5(GEMIN5),干扰素诱导蛋白5(IFIT5),la核糖核蛋白1(lARP1),Sm样蛋白(lSM1),甲基转移酶1(METTl1),核帽结合蛋白(NCBP)1,NCBP2,NCBP2样蛋白(NCBP2l),NOP2/Sun RNA甲基转移酶家族成员2(NSUN2),Nudix水解酶(NUDT)10,NUDT3,NUDT4,营养素1(SNUPN),WD重复结构域4(WDR4)]的表达水平,利用CCA患者临床数据进行生存分析。对所有患者数据进行共识聚类分析并筛选预后相关的差异表达基因(DEG),为了检测m7G甲基化调节因子及其DEG的影响进行共识聚类分析,进一步建立m7G甲基化评分系统,评估m7G甲基化评分与CCA患者预后的相关性。结果约48%(12/25)的m7G甲基化调节因子DNA拷贝数增加,其中AGO2的拷贝数增加程度最高;约56%(14/25)的m7G甲基化调节因子DNA拷贝数减少,其中EIF4G3的拷贝数减少程度最高,并且与患者预后密切相关;除IFIT5外,其余调节因子之间存在复杂且密切的相互作用关系。17个与CCA患者预后相关的m7G甲基化调节基因,其中lARP1、NUDT3、SNUPN、NCBP2、EIF4E1B、NUDT11、NSUN2、EIF4A1、GEMIN5和CYFIP1的高表达与患者预后正相关,EIF4E3、WDR4、EIF4E2、NUDT4、NCBP1、EIF4E和DCP2的高表达与患者预后不良相关(P<0.05)。通过对m7G甲基化分型之间取交集DEG,DEG主要富集于血管内皮细胞增殖与新生血管生成等。筛选预后相关基因,得到2个具有预后差异的m7G甲基化基因特征亚组,m7G甲基化评分高低两组分型之间差异有统计学意义,即m7G甲基化低评分组患者预后相对较好。结论研究表明,m7G甲基化修饰异常与CCA的发生发展密切相关,并且在肿瘤调控网络的形成中起着重要作用,评估肿瘤中的m7G甲基化修饰模式将有助于指导制定更有效的治疗策略。

关键词：胆管癌 m7G甲基化预后 m7G甲基化评分

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On multi-transitivity with respect to a vector

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Science China Mathematics 2014年第8期57卷 1639-1648页

作者： CHEN ZhiJing lI Jian l Jie Department of Mathematics Sun Yat-Sen University Department of Mathematics Shantou University School of Mathematics South China Normal University

A topological dynamical system(X,f)is said to be multi-transitive if for every n∈N the system(Xn,f×f2××fn)is *** introduce the concept of multi-transitivity with respect to a vector and show that multi-transitivity can be characterized by the hitting time sets of open sets,answering a question proposed by Kwietniak and Oprocha(2012).We also show that multi-transitive systems are li-Yorke chaotic.

关键词：载体拓扑动力系统传递系统物相

高龄妊娠对子代大鼠前额叶皮层神经干细胞增殖及突触可塑性的影响

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生殖医学杂志 2024年第5期33卷 635-640页

作者：田肖风赵鑫曾宪旭刘素娜吕书博石瑛石翠格郑州大学第三附属医院郑州450052 国家卫生健康委科学技术研究所北京100081

目的探究高龄妊娠对子代大鼠前额叶皮层神经干细胞增殖及突触可塑性的影响。方法选择3月龄与12月龄SD雌性大鼠,均与3月龄SD雄性大鼠合笼。子代鼠出生后,将其分为适龄子代组(Con组)和高龄子代组(AMA组)。取出生后1 d的子代大鼠脑组织,利... 详细信息

目的探究高龄妊娠对子代大鼠前额叶皮层神经干细胞增殖及突触可塑性的影响。方法选择3月龄与12月龄SD雌性大鼠,均与3月龄SD雄性大鼠合笼。子代鼠出生后,将其分为适龄子代组(Con组)和高龄子代组(AMA组)。取出生后1 d的子代大鼠脑组织,利用HE染色观察前额叶皮层神经元的形态变化;利用qRT-PCR和Western blot方法分别检测前额叶皮层中G蛋白信号调节蛋白2(RGS2)、巢蛋白(Nestin)、突触后致密区蛋白95(PSD95)、脑源性神经营养因子(BDNF)和突触素(SYN)的mRNA及其蛋白质表达变化情况。结果HE染色结果发现,与Con组比较,AMA组前额叶皮层的神经元数目减少,部分神经元发生变性,出现胞体肿胀、空泡化现象。qRT-PCR和Western blot结果发现,与Con组比较,AMA组Nestin、RGS2、PSD95、BDNF、SYN mRNA及蛋白质相对表达水平均显著下降(P<0.05)。结论高龄孕鼠子代前额叶皮层的神经干细胞增殖能力减弱,突触可塑性能力降低,可能是造成子代脑发育障碍和认知功能下降的原因之一。

关键词：高龄妊娠子代前额叶皮层突触可塑性神经干细胞

Study of Influence of Terrain and Climatic Factors on Groundwater-level Fluctuation in a Minor River Basin Using GIS

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Geo-Spatial Information Science 2011年第3期14卷 190-197页

作者： N.radhakrishnan l.Elango Geosensing Information Pvt.ltd. Department of Geology Anna University

Terrain environment parameters play a vital role in controlling groundwater movement:its recharge and discharge *** earlier studies have been conducted relating terrain parameters and groundwater condition using conventional me-thods and remote sensing *** study,however,endeavors to spatially visualize the degree of fluctuation in the ground-water level of Ongur,a minor river basin in different terrain units under different seasons(monsoon and summer) for three histori-cal periods of time using Geographic Information System(GIS) raster analysis.

关键词：地下水河盆地面环境参数季节的变化 GIS

Study on the pyroelectric properties of PlT/P(VDF-TrFE) nanocomposites

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Chinese Science Bulletin 1998年第2期43卷 111-114页

作者： Qingqi Zhang H. l. W. Chan Qifa Zhou C. l. Choy Hong Kong Polytech Univ Dept Appl Phys Hung Hom Hong Kong Hong Kong Polytech Univ Mat Res Ctr Hung Hom Hong Kong

lead lanthanum titanate (PlT) nanocrystalline powder prepared by the sol_gel process was incorporated into a polyvinylidene fluoride/trifluoroethylene (P(VDF_TrEF)) copolymer matrix to form PlT/P(VDF_TrFE) nanocomposites. The permittivities (ε) of the nanocomposites as a function of volume fraction of ceramic  were modeled using the Bruggeman model. The pyroelectric coefficients (p) and figure of merit (p/ε) were measured as functions of the poling field E. The nanocomposite has p and p/ε values higher than those of the copolymer, and has good potential in pyroelectric applications.

关键词： nanocomposites pyroelectric.

Presbyopic correction on the cornea

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Eye and Vision 2014年第1期1卷 24-33页

作者： Samuel Arba Mosquera Jorge l Alió SCHWIND eye-tech-solutions KleinostheimGermany Vissum Corporation AlicanteSpain Division of Ophthalmology Universidad Miguel HernándezAlicanteSpain.

Purpose:The aim of this systematic review was to synthesize and appraise the evidence of the benefits of presbyopic correction on the cornea for visual ***:Comprehensive search was conducted in MEDlINE using keywords like“presbylasik”,“presbyopic refractive surgery”,“corneal pseudoaccommodation”and“corneal multifocality”.We reviewed corrected and uncorrected visual acuities for distance and near(uncorrected distance visual acuity(UDVA),uncorrected near visual acuity(UNVA),corrected distance visual acuity(CDVA),distance corrected near visual acuity(DCNVA),corrected near visual acuity(CNVA)),along with the refractive outcomes in spherical equivalent(SE)and astigmatism comparing the differences observed between preoperative myopic and hyperopic patients,as well as among ***-one studies met the inclusion and quality *** provides excellent distance and near uncorrected acuities,but with a 17%retreatment and a 5%reversal *** multifocal ablations result in 12%loss of 2 or more lines of CDVA,and a 21%retreatment *** Blended Vision provides excellent UDVA,but with a 19%retreatment *** experiences with Supracor show moderate predictability and a 22%retreatment *** results in 9%loss of 2 or more lines of *** provides excellent UDVA,with only a 1%retreatment rate,but a 6%reversal *** experiences with PresbyMAX provided excellent UNVA and DCNVA,showing excellent predictability and a 1%reversal ***:The findings have implications for clinicians and policymakers in the health-care industry and emphasize the need for additional trials examining this important and widely performed clinical procedure.

关键词： Presbyopic refractive correction Presbylasik Corneal pseudoaccommodation Corneal multifocality Distance corrected near visual acuity Monovision laser Blended Vision Supracor and Intracor KAMra PresbyMAX

Antihypertensive prescribing patterns in non-dialysis dependent chronic kidney disease:Findings from the Salford Kidney Study

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World Journal of Nephrology 2023年第5期12卷 168-181页

作者： rajkumar Chinnadurai Henry H l Wu Jones Abuomar Sharmilee Rengarajan David I New Darren Green Philip A Kalra Donal O’Donoghue Renal Research Centre&Department of Renal Medicine Northern Care Alliance NHS Foundation TrustSalford M68HDUnited Kingdom Department of Renal Research Kolling Institute of Medical ResearchRoyal North Shore Hospital&The University of SydneySt.Leonards(Sydney)2065New South WalesAustralia Faculty of Biology Medicine&HealthThe University of ManchesterManchester M17HRUnited Kingdom

BACKGROUND Hypertension is commonly observed in patients living with chronic kidney disease(CKD).Finding an optimal treatment regime remains challenging due to the complex bidirectional cause-and-effect relationship between hypertension and *** remains variability in antihypertensive treatment *** To analyze data from the Salford Kidney Study database in relation to antihypertensive prescribing patterns amongst CKD *** The Salford Kidney Study is an ongoing prospective study that has been recruiting CKD patients since *** patients are followed up annually,and their medical records including the list of medications are updated until they reach study endpoints[starting on renal replacement therapy or reaching estimated glomerular filtration rate(eGFR)expressed as ml/min/1.73 m2≤10 ml/min/1.73 m2,or the last follow-up date,or data lock on December 31,2021,or death].Data on antihypertensive prescription practices in correspondence to baseline eGFR,urine albumin-creatinine ratio,primary CKD aetiology,and cardiovascular disease were *** between patients who were prescribed three or more antihypertensive agents and their clinical outcomes were studied by Cox regression ***-Meier analysis demonstrated differences in survival *** Three thousand two hundred and thirty non-dialysis-dependent CKD patients with data collected between October 2002 and December 2019 were *** median age was 65 years.A greater proportion of patients were taking three or more antihypertensive agents with advancing CKD stages(53%of eGFR≤15 ml/min/1.73 m2 vs 26%of eGFR≥60 ml/min/1.73 m2,Plasses of antihypertensive agents was observed as the urine albumin-creatinine ratio category increased(category A3:62%vs category A1:40%,Plarly noticeable in the number of individuals prescribed renin angiotensin system *** pr

关键词： Hypertension Chronic kidney disease Antihypertensive agents Prescribing patterns Cardiovascular complications Renin angiotensin system blockers

Physical activity and cognitive function among older adults in China:A systematic review

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Journal of Sport and Health Science 2016年第3期5卷 287-296,383页

作者： Jiaojiao lü Weijie Fu Yu liu Key laboratory of Exercise and Health Sciences of Ministry of Education Shanghai University of Sport

Background: Physical activity(PA) has been shown to benefit cognitive function in older adults. However, the cognitive benefits of exercising for older Chinese adults have not been systematically documented. This study was to conduct a systematic review on evidence that PA is beneficial for cognitive functioning in older Chinese ***: Major databases, including Pub Med, EMBASE, Cochrane library, Wan Fang, CNKI, and VIP, were searched for studies published in English or Chinese between January 2000 and December 2015. randomized and non-randomized controlled trials(RCTs and non-RCTs), cohort,case-control and cross-sectional studies that evaluated PA and cognitive function among older Chinese adults were included in this ***: Of 53 studies included and reviewed, 33 were observational(22 cross-sectional, 7 case-control, and 4 cohort) and 20 were experimental(15 RCTs, 5 non-RCTs). Observational studies showed an association of reduced risk of cognitive-related diseases(i.e., mild cognitive impairment, Alzheimer's disease, and dementia) through PA, whereas experimental studies reported exercise-induced improvement in multiple domains of cognitive function(i.e., global cognitive function, memory, executive function, attention, language, and processing recourse).Conclusion: This systematic review provides initial evidence that PA may benefit cognition in older Chinese adults. Further studies of individuals with cognitive impairments and prospective and RCT studies having high scientific rigor are needed to corroborate the findings reported in this review.

关键词： Chinese elderly Cognition Dementia Exercise Public health Systematic review

不同加工条件莲蓉馅料感官评价与仪器测试指标的相关性研究

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中国食品添加剂 2024年第8期35卷 178-187页

作者：赵利平梁梓健吕义忠谢雅曼李玉坤吴锦源高向阳广州酒家集团利口福食品有限公司广州511442 广州市广式焙烤食品加工工程技术研究开发中心广州511442 华南农业大学食品学院广东省功能食品活性物重点实验室广州510642 广州酒家集团股份有限公司广州510170

针对目前莲蓉馅料缺乏全面客观的品质评价方法,通过研究蒸煮工序和磨浆工序对莲蓉馅料品质的影响,确定粒度分布、流变特性和质构特性等仪器分析指标可用于表征莲蓉馅料感官品质。结果表明,延长蒸煮时间和磨浆时间对仪器测试指标均有影... 详细信息

针对目前莲蓉馅料缺乏全面客观的品质评价方法,通过研究蒸煮工序和磨浆工序对莲蓉馅料品质的影响,确定粒度分布、流变特性和质构特性等仪器分析指标可用于表征莲蓉馅料感官品质。结果表明,延长蒸煮时间和磨浆时间对仪器测试指标均有影响。感官及仪器分析结果表明,改变蒸煮和磨浆时间均可影响质地口感。“细腻顺滑”与粒度分布、TPA部分指标存在显著相关性(P<0.05);“软绵”与流变特性、TPA部分指标存在显著相关性(P<0.05),说明仪器分析指标可一定程度上反映莲蓉馅料口感质地。构建了莲蓉馅料感官评价指标的预测模型,得到“细腻顺滑”、“软绵”描述词指标的回归方程分别为Y=19.19-0.14X1-0.04X2、Y=8.26-0.03X1+0.09X2-0.032X3,为使用仪器分析指标定量表达莲蓉馅料质地提供了理论依据。

关键词：莲蓉馅料粒度分布流变分析质构分析感官评价

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