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Does climate change alter the nutrient trends of Cedrela fissilis *** in the southern Brazilian Amazon?

Ecological Processes 2023年第1期12卷 806-827页

作者： Daigard R.Ortega Rodriguez raúl sánc.ez-salguero Andrea Hevia Renata C.Bovi Marciel J.Ferreira James H.speer Fidel A.Roig Mario Tomazello-Filho Escola superior de Agricultura luiz de Queiroz Departamento de Ciências FlorestaisUniversidade de São PauloAv.Pádua Dias 11PiracicabaSão Paulo 13418-900Brazil DendrOlavide-Department sistemas Físicos Químicos y NaturalesUniversidad Pablo de OlavideCrta.Utrera Km.141013 SevilleSpain Department of Ecology Universidad de JaénCampus Las Lagunillas s/n.23009 JaénSpain Departamento de Ciência Florestal Solos e AmbienteUniversidade Estadual PaulistaAv.Universitária 3780BotucatuSão Paulo 18610-034Brazil laboratory of silviculture Universidade Federal do Ama-zonasAv.Gen.Rodrigo Octávio 6200ManausAmazonas 69080-900Brazil Department of Earth and Environmental systems Indiana State UniversityTerre MauteIN 47809USA Argentine Institute of Nivology Glaciology and Environmental Sciences(IANIGLA CONICETUniversidad Nacional de Cuyo)5500 MendozaArgentina Hémera Centro de Observación de la Tierra Escuela de Ingeniería ForestalFacultad de CienciasUniversidad MayorSantiagoChile

Background The increase in the frequency and intensity of droughts is pointed out as one of the main factors altering biogeochemical cycles in the Amazon *** eco-nutritional approach using X-ray fluorescence micro-analysis(μXRF)is proposed to verify the long-and short-term effects of droughts on the growth and xylem nutrient concentrations of Cedrela fissilis *** Fourteen radii were selected from a tree-ring width chronology and X-rayed by Itrax *** of ring width,wood density,and concentrations of aluminum(Al),phosphorus(P),sulfur(s),calcium(Ca),potassium(K),manganese(Mn),iron(Fe)and strontium(sr)together with Al/Ca,Ca/Mn,K/Ca,sr/Ca and Mn/s ratios were constructed and correlated with precipitation,temperature,the difference between precipitation and potential evapotranspiration(P-PET)and standardized precipitation-evapotranspiration index(sPEI).Results During dry years,*** showed narrower,less dense rings,lower Al,P,s and Ca,and higher K and Fe concentrations(the opposite was found in wet years).Ring width decreased(together with Al,P,s,K,Ca,Mn,Fe,sr,Al/Ca,K/Ca and sr/Ca)and wood density increased(together with Ca/Mn and Mn/s),which was associated with an increase in evapotranspiration and temperature over time,mainly since *** fissilis showed a tendency to increase its capacity for resistance,and a recovery and resilience in growth over time associated with responses in Al,Ca,P and ***,it showed a risk in the capacity for recovery of the pre-drought density values,associated with unsatisfactory responses in Al,Ca,K,Fe and *** This study is the first attempt to analyze tree-ring nutritional evidences of *** trees to climate sensitivity and resilience to drought,based on long-term data from seasonal moist tropical forests of the *** data suggested that *** is undergoing alterations in the concentration,use and redistribution of nutrients associated with increasing wood density and d

关键词： Dendrochemistry μXRF Drought Tropical tree Eco-chemical indicator Resilience Resistance Recovery

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Robust transcriptional indicators of immune cell death revealed by spatiotemporal transcriptome analyses

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Molecular Plant 2022年第6期15卷 1059-1075页

作者： Jose salguero-linares Irene serrano Nerea Ruiz-solani Marta salas-Gómez Ujjal Jyoti Phukan Victor Manuel González MartíBernardo-Faura Marc Valls David Rengel Nuria s.Coll Centre for Research in Agricultural Genomics(CraG) CSIC-IRTA-UAB-UBCampus UABBellaterra08193 BarcelonaSpain lIPM Universitéde ToulouseINRACNRS84195 Castanet-TolosanFrance INraE GeT-PlaGeGenotoul31326 Castanet-TolosanFrance Department of Genetics Universitat de Barcelona08028 BarcelonaSpain Consejo superior de Investigaciones Científicas(CsIC) BarcelonaSpain Department of Plant Molecular Biology and Physiology.Albrechtvon Haller Institute for Plant sciences niversity of GottingenJulia-Lermontowa-Weg3.37077 GottingenGrmany Institut de Phammacologie et de Biologie structurale IPBs Universite deToulouse CNRSUPSBP 64182205 route de Nartxonne310077 Toulouse Cedex 04France

Recognition of a pathogen by the plant immune system often triggers a form of regulated cell death traditionally known as the hypersensitive response(HR).This type of cell death occurs precisely at the site of pathogen recognition,and it is restricted to a few *** research has shed light on how plant immune receptors are mechanistically ***,two central key questions remain largely unresolved:how does cell death zonation take place,and what are the mechanisms that underpin this phenomenon?Consequently,bona fide transcriptional indicators of HR are lacking,which prevents deeper insight into its mechanisms before cell death becomes macroscopic and precludes early or live *** this study,to identify the transcriptional indicators of HR we used the paradigmatic Arabidopsis thaliana–Pseudomonas syringae pathosystem and performed a spatiotemporally resolved gene expression analysis that compared infected cells that will undergo HR upon pathogen recognition with bystander cells that will stay alive and activate *** data revealed unique and time-dependent differences in the repertoire of differentially expressed genes,expression profiles,and biological processes derived from tissue undergoing HR and that of its ***,we generated a pipeline based on concatenated pairwise comparisons between time,zone,and treatment that enabled us to define 13 robust transcriptional HR *** these genes,the promoter of an uncharacterized AAA-ATPase was used to obtain a fluorescent reporter transgenic line that displays a strong spatiotemporally resolved signal specifically in cells that will later undergo pathogen-triggered cell *** valuable set of genes can be used to define cells that are destined to die upon infection with HR-triggering bacteria,opening new avenues for specific and/or high-throughput techniques to study HR processes at a single-cell level.

关键词： Arabidopsis thaliana cell death indicator effector-triggered immunity hypersensitive response pattern-triggered immunity plant immunity Pseudomonas syringae

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Acute hepatitis with autoimmune features after COVID-19 vaccine:coincidence or vaccine-induced phenomenon?

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Gastroenterology Report 2022年第1期10卷 628-631页

作者： Jose M.Pinazo-Bandera Alicia Hernandez-Albujar Ana Isabel Garcia-salguero Isabel Arranz-salas raul J.Andrade Mercedes Robles-Diaz Division of Gastroenterology and Hepatology Instituto de Investigacion Biomedica de Malaga-IBIMAHospital Universitario Virgen de la VictoriaUniversidad de MalagaMalagaSpain Division of Anatomical Pathology Hospital Universitario Virgen de la VictoriaMalagaSpain Centro de Investigacion Biomedica en Red de Enfermedades Hepaticas y Digestivas(CIBERehd) MadridSpain

Introduction Autoimmune diseases result from a breach of immunological self-tolerance and tissue damage by autoreactive T *** acute respiratory syndrome coronavirus-2(sARs-CoV-2)infection is characterized by an inflam... 详细信息

关键词： autoimmune vaccine acute

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黄麻AREB/ABF基因家族的鉴定及其在外源ABA作用下盐和干旱胁迫中的表达

黄麻AREB/ABF基因家族的鉴定及其在外源ABA作用下盐和干旱胁迫中...

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作者： Fiallos salguero Manuel sebastian 福建农林大学

学位级别：硕士

黄麻(Corchorus l.)是锦葵科黄麻属一年生草本作物,因产量高、用途广泛而成为继棉花后最重要的天然纤维作物。近年来,由于土壤盐碱化以及干旱的加重,黄麻纤维产量和品质均有所下降,从而对世界范围内黄麻产业的发展造成了较严重的影响。A... 详细信息

黄麻(Corchorus l.)是锦葵科黄麻属一年生草本作物,因产量高、用途广泛而成为继棉花后最重要的天然纤维作物。近年来,由于土壤盐碱化以及干旱的加重,黄麻纤维产量和品质均有所下降,从而对世界范围内黄麻产业的发展造成了较严重的影响。AREB/ABF(ABA响应元件结合蛋白/AREB结合因子)基因家族在ABA介导的信号传导途径中发挥重要作用,它能增强植物对环境胁迫的适应性。首先,本研究分析了施用和不施用外源ABA情况下,盐和干旱胁迫对翠绿(Cl)和南阳长果(nc)2个黄麻品种苗期形态特征的影响。同时,采用MEGA-X构建了ABF蛋白序列的进化树,并通过Pfam和sMART进行了蛋白结构预测和Ka/Ks分析。此外,用BlAsT对AREB/ABF基因家族进行了鉴定,并采用q RT-PCR技术,对其在外源ABA作用下盐(sT)和干旱(PEG)胁迫中不同组织、不同时间点的基因表达进行了分析。研究结果表明,品种翠绿对盐和干旱胁迫的抗性优于南阳长果。同时,与未施加外源ABA的胁迫条件下的幼苗相比,2个品种幼苗的长势在sT+ABA处理和PEG+ABA处理下性状表现更好,它们的株高、存活率等性状显著改善。另外,在黄麻基因组中共鉴定到8个CoABF基因。对来自7个不同物种(拟南芥、水稻、红麻、棉花、可可、圆果种和长果种黄麻)的90个AREB/ABF蛋白序列构建了系统发育树,它们可被分为4组(A,B,C和D)。大多数CoABF蛋白与圆果种黄麻聚在一起,并与可可有共同的祖先,其中CoABF1蛋白仅与可可有关。基因结构分析显示,除了D组,每组外显子/内含子的分布均是保守的。另外,从CoABF蛋白中预测了保守的基序,发现基序1和2都具有强保守性的b ZIP结构域。此外,CoABF基因中顺式元件的预测显示了其对光照、激素和逆境的响应。分析显示CoABF1、3、7和8诱导了AREB的反应,从而增加了植物中脱落酸的表达。基因复制的结果表明了Ka/Ks比率为0.25的两个基因的相互作用,揭示了其具有强烈的纯化选择和比较古老的分化时间。同时,AREB/ABF成员在黄麻——棉花和黄麻——可可之间都有明确的纯化选择,并且与棉花的差异时间比可可更古老。对CoABF基因在翠绿和南阳长果的不同组织(叶、茎和根)、不同胁迫时间(2h、4h、8h、12h和24h)、5个处理(ABA、PEG、PEG+ABA、sT和sT+ABA)的实时表达进行了分析。结果表明,在ABA处理中CoABF3和CoABF7表达水平最高。而且在PEG+ABA处理下的品种翠绿中,这两个基因在胁迫后显著表达,特别是在叶和茎中,其中CoABF3分别在胁迫后12h的叶片和4h的茎中表达水平较高,而CoABF7在叶片和茎中显著表达的时间点分别为胁迫后2h和8h。同时,CoABF3仅在南阳长果的茎中表达,在PEG+ABA处理后8h表现出最高的活性。此外,与PEG胁迫下的表达相比,PEG+ABA处理中这两个基因在两个品种中均表现上调。相反,这两个基因在sT和sT+ABA下的翠绿幼苗中表达水平差别不大。另外,在南阳长果中,CoABF3和CoABF7在sT+ABA下的茎和根中被诱导表达,并且在胁迫后8h达到最大值。通过研究我们发现,当外源ABA存在时,CoABF3和CoABF7在盐和干旱胁迫处理的黄麻幼苗中显著表达,揭示了ABA信号通路在胁迫处理下的黄麻中发挥了作用,研究结果可为利用分子生物学手段创造抗逆性黄麻新种质提供理论依据。

关键词：黄麻(Corchorus olitorius l.) AREB/ABF基因脱落酸盐胁迫干旱胁迫基因表达

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提高肉鸡饲料转化率的营养措施

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中国家禽 2013年第12期35卷 42-44页

作者： s.salguero l.F.T.Albino P.B.Rodrigues H.s.Rostagno 曹玉娟巴西维索萨联邦大学

饲料成本占肉鸡总生产成本的70%,因而减少饲料成本一直是肉鸡生产者所面临的问题。NRC、FEDNA、INra及巴西家禽饲料成分与营养需求表为全世界的家禽营养工作者提供统一的营养参考标准,本文利用巴西家禽饲料成分与营养需求表中的公式及... 详细信息

饲料成本占肉鸡总生产成本的70%,因而减少饲料成本一直是肉鸡生产者所面临的问题。NRC、FEDNA、INra及巴西家禽饲料成分与营养需求表为全世界的家禽营养工作者提供统一的营养参考标准,本文利用巴西家禽饲料成分与营养需求表中的公式及推荐量,参考最新相关数据和方法来提高肉鸡的饲料转化率。

关键词：肉鸡饲料转化率营养

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Reverse Engineering Based Methodology for Redesigning Contour Milling Tools

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World Journal of Engineering and Technology 2016年第3期4卷 206-212页

作者： A. P. Valerga M. Batista J. salguero A. Gomez-Parra M. Marcos Mechanical Engineering and Industrial Design Department Faculty of Engineering University of Cadiz Puerto Real Cádiz Spain

Reverse Engineering (RE) involves the use of techniques aimed to retrieve information about manufactured products, not only regarding geometries, but also materials and functionality. Today, even if several RE techniques are known, many of them still leave the object unusable to analyze. Nevertheless, other alternatives to this problem allows for obtaining a Digital or Virtual Model (VM) via the three-dimen- sional scanning. Getting the VM of an item, via scanning or not, can offer many possibilities to digital analysis (FEM). Furthermore, starting from VM, it is possible to achieve the physical reproduction of an element, part or workpiece—in the same or different materials—using Additive Manufacturing (AM) technologies. This enables to improve the product through a redesign process. In this paper, a RE based methodology is proposed for redesigning a tool for contour milling, after comparing different microscopy based techniques, 3D-scanner tools and CAD-CAGD utilities for generating a Virtual Model of the newly designed mill.

关键词： Reverse Engineering Cutting Tools simulation 3D scanning Modelling

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Methodology for the study of the Quality of CFRP Dry Drilling Based on Macrogeometrical and Dimensional Deviations

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World Journal of Engineering and Technology 2016年第3期4卷 200-205页

作者： J. salguero s. R. Fernandez-Vidal P. Mayuet J. M. Vazquez-Martinez M. Alvarez M. Marcos Mechanical Engineering and Industrial Design Department University of Cadiz Cadiz Spain

Commonly, Carbon Fiber Reinforced Plastics (CFRP) aerospace elements need to be machined (drilling) for subsequent assembly operations, mainly riveting, taking a critical importance the quality requirements of the holes. some of the adjustment defects that can be produced during the dry drilling of CFRP are related to macrogeometrical (diameter, D, and cylindricity, DC) deviations. This paper reports on the results of a study of the influence of the cutting parameters V (cutting speed) and f (feedrate) on the D and DC deviations in the dry drilling of CFRP. A particular procedure based on plastic hole replicas has been performed. Diameters deviations were acceptable in all the cases studied. Because of this, DC is the controlling factor for accepting the drilled holes. In the studied range, lowest cutting speeds and highest feedrates have allowed obtaining the minimum cylindricity deviation.

关键词： Dry Drilling CFRP Macrogeometrical Deviations Cylindricity Diameter

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Role of nitric oxide in the maintenance of pluripotency and regulation of the hypoxia response in stem cells

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World Journal of stem Cells 2015年第3期7卷 605-617页

作者： Amparo Beltran-Povea Estefania Caballano-Infantes Carmen salguero-Aranda Franz Martín Bernat soria Francisco J Bedoya Juan R Tejedo Gladys M Cahuana Andalusian Center for Molecular Biology and Regenerative Medicine-University Pablo de Olavide CIBERDEM RED-TERCEL 41092 Seville Spain Andalusian Center for Molecular Biology and Regenerative Medicine (CABIMER) -Fundación Progreso y salud CIBERDEM RED-TERCEL 41092 Seville Spain

stem cell pluripotency and differentiation are global processes regulated by several pathways that have been studied intensively over recent years. Nitric oxide(NO) is an important molecule that affects gene expression at the level of transcription and translation and regulates cell survival and proliferation in diverse cell types. In embryonic stem cells NO has a dual role, controlling differentiation and survival, but the molecular mechanisms by which it modulates these functions are not completely defined. NO is a physiological regulator of cell respiration through the inhibition of cytochrome c oxidase. Many researchers have been examining the role that NO plays in other aspects of metabolism such as the cellular bioenergetics state, the hypoxia response and the relationship of these areas to stem cell stemness.

关键词： Pluripotency Differentiation Nitric oxide Metabolism Hypoxia stem cell

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