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Notch2 regulates matrix metallopeptidase 9 via PI3K/AKT signaling in human gastric carcinoma cell MKN-45

World Journal of Gastroenterology 2012年第48期18卷 7262-7270页

作者： Ling-Yun guo Yu-Min Li Liang Qiao Tao Liu Yuan-Yuan Du jun-Qiang Zhang Wen-Ting He Yong-Xun Zhao Dong-Qiang He the Second Clinical Medical School of Lanzhou University the Second Hospital of Lanzhou University Key Laboratory of Digestive System Tumors Department of General Surgerythe Second Hospital of Lanzhou University Storr Liver Unit at the Westmead Millennium Institute the University of Sydney at Westmead Hospital Key Laboratory of Digestive System Tumors the Second Hospital of Lanzhou University Department of Oncological Surgery the First Hospital of Lanzhou University

AIM：To clarify the role of activated Notch2 in the invasiveness of gastric ***：To investigate the invasiveness of silencing Notch2 gene expression,we established a Notch2small interfering RNA（siRNA） transfected cell line using the MKN-45 gastric cancer cell *** the successful transfection confirmed by real-time reverse transcription-polymerase chain reaction（RT-PCR） and Western blotting,migration and invasion assays were employed to evaluate the aggressiveness of the gastric ***-PCR and Western blottings were employed to confirm the down-regulation of Notch2 and to evaluate the expression of epithelial mesenchymal transition-related gene matrix metallopeptidase 9（MMP9）,Akt,*** confirm the relationship between PI3KAkt and MMP9,the PI3K inhibitor LY294002 was used to treat MKN-45 ***：Notch2 expression was dramatically decreased after Notch2 siRNA transfection（100.00% ± 9.74% vs 11.61% ± 3.85%,P 〈 0.01 by qRT-PCR）.There was also a marked reduction of Notch target gene Hes1（100.00% ± 4.74% vs 61.61% ± 3.58%,P 〈 0.05） at the mRNA,indicating an inhibition of Notch *** of Notch signaling was also confirmed by the marked reduction of Notch2 intracellular domain at the protein levels（100.00% ± 9.74% vs 65.61% ± 7.58%,P 〈 0.05）.Down-regulation of Notch2 by siRNA enhanced tumor cell invasion（100.00% ± 21.64% vs 162.22% ± 16.84%,P 〈 0.05） and expression of MMP9（1.56 fold,P 〈 0.05）,and activated the pro-MMP9 protein to its active form（1.48 fold,P 〈 0.05）.There was no significant difference in the protein levels of Akt between the two groups（100.00% ± 10.87% vs 96.61% ± 7.33%,P 〉 0.05）,while down-regulation of Notch2 elevated p-Akt expression（100.00% ± 9.87% vs 154.61% ± 13.10%,P 〈 0.05）.Furthermore,p-Akt and MMP9 was down-regulated in response to the inhibitor LY294002（p-Akt 100.00% ± 8.87% vs 58.27% ± 5.01%,P 〈 0.05;MMP9 100.00% ± 9.17% vs 50.03% ± 4.88%,P 〈 0.05）.CONCLUSION：Notch2 may negatively regulate cell invasion by

关键词： Notch2 Stomach Cancer Invasion Epithelial mesenchymal transition Matrix metallopeptidase 9 RNA interference

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The Influence of Secular Trends in Body Height and Weight on the Prevalence of Overweight and Obesity among Chinese Children and Adolescents

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Biomedical and Environmental Sciences 2016年第12期29卷 849-857页

作者： FU Lian guo SUN Li Li WU Shao Wei YANG Yi De LI Xiao Hui WANG Zheng He WU Lu WANG Fu Zhi MA jun Department of Preventive Medicine Bengbu Medical College Department of Environmental Health Harvard T.H.Chan School of Public Health Institute of Child and Adolescent Health School of Public HealthPeking University Health Science Center

Objective To explore the influence of secular trends in body height and weight on the prevalence of overweight and obesity among Chinese children and adolescents. Methods The data were obtained from five cross-sectional Chinese National Surveys on Students＇ Constitution and Health. Overweight/obesity was defined as BMI-for-age Z-score of per the Wold Health Organization （WHO） reference values. Body height and weight for each sex and age were standardized to those reported in 1985 （standardized height＂ SHY, standardized weight： SWY） and for each sex and year at age 7 （standardized height： SHA; standardized weight： SWA） using the Z-score method. Results The prevalence of overweight/obesity in Chinese children was 20.2% among boys and 10.7% among girls in 2010 and increased continuously from 1985 to 2010. Among boys and girls of normal weight, SHY and SHA were significantly greater than SWY and SWA, respectively （P 〈 0.001）. Among boys and girls with overweight/obesity, SHY was significantly lower than SWY （P 〈 0.001）, and showed an obvious decreasing trend after age 12. SHA was lower than SWA among overweight boys aged 7-8 years and girls aged 7-9 years. SHY/SHW and SHA/SWA among normal-weight groups were greater than among overweight and obese groups （P 〈 0.001）. Conclusion The continuous increase in the prevalence of overweight/obesity among Chinese children may be related to a rapid increase in body weight before age 9 and lack of secular increase in body height after age 12.

关键词： Obesity Children Secular trends Body height Body weight

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语言态度与方言变异——溧水县城居民语言态度与语言使用情况的简要调查

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南京社会科学 2007年第8期 134-140页

作者：郭骏南京晓庄学院人文学院高级讲师博士南京211171

本文通过调查发现:(1)"街上话"与"乡下话"构成城镇方言与乡村方言的二元对立;(2)溧水县城居民对在城话正在发生变化有着普遍的认同,说明在城话正处于一个不断向普通话靠拢的发展变化过程中;(3)在城话与普通话的同时使用与双项认同说明... 详细信息

本文通过调查发现:(1)"街上话"与"乡下话"构成城镇方言与乡村方言的二元对立;(2)溧水县城居民对在城话正在发生变化有着普遍的认同,说明在城话正处于一个不断向普通话靠拢的发展变化过程中;(3)在城话与普通话的同时使用与双项认同说明县城居民的语言生活是一种在城话与普通话并存共用、以在城话为主导的双语制,这使得在城话与普通话有面对面接触的机会,再加上居民对普通话的权威地位有着极高的认同度,自然就会有意或无意地将自己所说的在城话向普通话靠拢,可见在城话与普通话并存共用的双语制决定了在城话向普通话靠拢的演变方向。

关键词：溧水在城话南京话普通话语言态度方言变异

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Localization of cystathionine β synthase in mice ovaries and its expression profile during follicular development

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Chinese Medical Journal 2006年第22期119卷 1877-1883页

作者： LIANG Rong YU Wei-dong DU jun-bao YANG Li-jun SHANG Mei guo Jing-zhu Department of Pediatrics People＇s Hospital Peking University Beijing 100044 China Center of Laboratory People＇s Hospital Peking University Beijing 100044 China Department of Pediatrics First Hospital Peking University Beijing100034 China

Background In vitro fertilization （IVF） researches have suggested that cystathionine β synthase （CBS） is involved in oocyte development. However, little is known about the regional and cellular expression patterns of CBS in the ovary. The purpose of this study was to analyze the localization of CBS in mice ovaries and to investigate the expression profile during follicular development. Methods We used in situ hybridization and immunohistochemical analysis to determine CBS expression in the ovaries of female Balb/c mice. Then the follicles were collected from F1 （C57BL×Balb/c） mice and cultured in vitro. With the method of semi-quantitative RT-PCR, we also investigated the expression profile of CBS during follicular development. Results CBS was absent in the oocytes, although it was ubiquitously expressed in the ovary with the strongest expression in follicular cells at all stages. In late antral follicles, CBS expression was markedly higher in granulosa cells located close to the antrum and in cumulus cells around the oocyte. The semi-quantitative RT-PCR showed that CBS mRNA was detected in follicles at all stages in vitro. In cumulus-oocyte complexes superovulated, CBS expression also increased rapidly. Conclusions CBS was located mainly in the follicular cells in the ovaries. The level of CBS expression is high in follicles during folliculogenesis in mice. Differences in the CBS expression profile between oocyte and follicular cells suggest a role for CBS as a mediator in interactions between oocyte and granulosa cells.

关键词： cystathionine β synthase oocytes ovarian follicles homocysteine

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A Reverse-transcription Recombinase-aided Amplification Assay for the Rapid Detection of the Far-Eastern Subtype of Tick-borne Encephalitis Virus

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Biomedical and Environmental Sciences 2019年第5期32卷 357-362页

作者： WANG Qian Ying LI Fan SHEN Xin Xin FU Shi Hong HE Ying LEI Wen Wen LIANG guo Dong WANG Huan Yun MA Xue jun Department of Viral Encephalitis NHC Key Laboratory of BiosafetyNational Institute for Viral Disease Control and PreventionChinese Center for Disease Control and PreventionBeijing 102206China State Key Laboratory for Infectious Disease Prevention and Control Chinese Center for Disease Control and PreventionBeijing 102206China NHC Key Laboratory of Medical Virology and Viral Diseases National Institute for Viral Disease Control and PreventionChinese Center for Disease Control and PreventionBeijing 102206China

Objective Tick-borne encephalitis virus(TBEV) is an emerging pathogen in Europe and North Asia that causes tick-borne encephalitis(TBE). A simple, rapid method for detecting TBEV RNA is needed to control this disease. Methods A reverse-transcription recombinase-aided amplification(RT-RAA) assay was developed. This assay can be completed in one closed tube at 39℃ within 30 minutes. The sensitivity and specificity of RT-RAA were validated using non-infectious synthetic RNA representing a fragment of the NS5 region of the wild-type(WT) TBEV genome and the Senzhang strain. Additionally, 10 batches of tick samples were used to evaluate the performance of the RT-RAA assay. Results The analytical limit of detection of the assay was 20 copies per reaction of the TBEV synthetic transcript and 3 plaque-forming units(pfu) per reaction of TBEV titers. With the specific assay, no signal due to other arboviruses was observed. Of the 10 batches of tick samples obtained from the Changbai Mountains of China, three were TBEV-positive, which was consistent with the results of the quantitative real-time PCR assay. Conclusion A rapid, highly sensitive, specific, and easy-to-use method was developed for the detection of the TBEV Far-Eastern subtype.

关键词： Tick‐borne encephalitis virus Subtype Far‐eastern Detection RT‐RAA

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The prognostic factors of effective ketoconazole treatment for metastatic castration-resistant prostate cancer： who can benefit from ketoconazole therapy？

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Asian Journal of Andrology 2012年第5期14卷 732-737页

作者： guo-Wen Lin Xu-Dong Yao Ding-Wei Ye Yao Zhu Shi-Lin Zhang Bo Dai Hai-Liang Zhang Yi-jun Shen Chun-Guang Ma Department of Urology Fudan University Shanghai Cancer CenterShanghai 200032China Department of Oncology Shanghai Medical CollegeFudan UniversityShanghai 200032China

We investigated the prognostic value of some variables of effective ketoconazole treatment for metastatic castration-resistant prostate cancer （mCRPC）. In total, 163 patients with mCRPC were eligible, receiving ketoconazole 200-400 mg three times daily with replacement doses of prednisone. Progression-free survival （PFS） was calculated from the beginning of the ketoconazole therapy to the onset of disease progression. The prognostic value of different variables for PFS was assessed by Cox regression analysis. The median PFS was 2.6 months （0.5-8.6 months） for these patients. The serum testosterone level changed during therapy, which decreased when the prostate-specific antigen （PSA） declined; the serum testosterone level increased as the levels of PSA relapsed. The median PFS values for patients associated with different factors were the following： 1.4 and 3.5 months for a nadir PSA of ≥ 0.2 and 〈0.2 ng ml- 1, respectively （hazard rate （HR）=4.767, P〈0.001）; 3.1 and 1.6 months for a baseline testosterone of ≥0.1 and 〈0.1 ng m1-1, respectively （HR=2.865, P=0.012）; 2.8 and 1.9 months for a baseline haemoglobin of ≥ 120 and 〈120 g 1-1, respectively （HR= 1.605, P〈0.001）; and 3.0 and 1.9 months for a PSA doubling time （PSADT） of ≥ 2.0 and 〈2.0 months, respectively （HR= 1.454, P=-0.017）. A risk model was constructed according to the four factors that divided patients into three subgroups of low risk （0-1 factors）, moderate risk （2 factors） and high risk （3-4 factors） with PFS values of 3.6, 3.0 and 1.4 months, respectively （HR=1.619, P〈0.001）. A nadir PSA of ≥0.2 ng m1-1, a baseline testosterone of 〈0.1 ng m1-1, a baseline haemoglobin of 〈 120 g I- 1 and a PSADT of 〈2 months were associated with a poor PFS. This risk model could provide evidence to predict the survival benefit of ketoconazole therapy.

关键词： castration-resistant prostate cancer ketoconazole therapy predictor progression-free survival

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德里达版本的《哈姆莱特》或解构版本的马克思主义——解读德里达《马克思的幽灵们》

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外国文学 2007年第5期 91-99页

作者：郭军北京语言大学外国语学院英语系北京100083

德里达在对马克思主义沉默多年后,于1993年公开谈论马克思主义,并于1994年出版了《马克思的幽灵们》,但这是一种解构版本的马克思主义,旨在激活马克思主义批判和变革世界的精神,将马克思主义从极权主义、教条主义的阐释中解脱出来,变成... 详细信息

德里达在对马克思主义沉默多年后,于1993年公开谈论马克思主义,并于1994年出版了《马克思的幽灵们》,但这是一种解构版本的马克思主义,旨在激活马克思主义批判和变革世界的精神,将马克思主义从极权主义、教条主义的阐释中解脱出来,变成一种不断革命论和未来学。他将这种精神的特征阐释为"徘徊的幽灵"。对此,他是借用《哈姆莱特》中鬼魂和与鬼魂打交道的几个人物的意象来展开其论述的,通过对莎士比亚的作品与马克思的几部经典著作的交错解读,阐述了对马克思的精神、对欧洲知识分子的责任、对当下时代、特别是对马克思关于"变革时代"的指令的独到思索,回答了全球化语境中"马克思主义何处去"和"马克思主义衰亡了吗"的问题。

关键词：《马克思的幽灵们》《哈姆莱特》解构与马克思主义批判与向往

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Characteristics of the Bibliometric Indexes of Chinese Core Journals on Environmental and Occupational Medicine

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环境与职业医学 2010年第9期27卷 580-582页

作者： HONG Qi ZHOU Zhi-jun DING Jin-yu WANG Xiao-yu guo Wei-wei Editorial Office of Journal of Environmental and Occupational Medicine Shanghai Municipal Center for Disease Control and Prevention Shanghai 200336 China School of Public Health Fudan University Shanghai 200032 China.

This paper investigated the current characteristics and changing trends of major journals on Chinese environmental and occupational medicine in order to promote the development of these journals. This study focused on the seven core journals in the area of environmental and occupational medicine listed on the Chinese Scientifi c and Technical Journal Citation Reports (2008 version). These journals were China Occupational Medicine, Chinese Journal of Industrial Hygiene and Occupational Diseases, Chinese Journal of Industrial Medicine, Industrial Health and Occupational Diseases, Journal of Environment and Health, Journal of Environmental and Occupational Medicine, Occupation and Health. Various observations were based on data from *** and Chinese Scientif ic and Technical Journal Citation Reports (2008 version), including cover of the journal, issue period, exposure to the internet, level of open source, impact factor, cited half-life, web immediacy index and rate of downloads per year. The outcomes showed that in appearance, the overall characteristics were continuous improvement of the covers, issue period, and moving toward the internet. In terms of contents, all factors had been improving. Nevertheless the level of influence was not very stable. The use of internet and the level of resource openness still have a long way to go to meet the international standard. There is still a huge gap for improvement. The contents and the appearance both need to be improved to serve the ever developing environmental and occupational medicine career.

关键词： environment occupation medicine core journal development bibliometric indexes

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Thioredoxin and impaired spatial learning and memory in the rats exposed to intermittent hypoxia

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Chinese Medical Journal 2012年第17期125卷 3074-3080页

作者： YANG Xiu-hong LIU Hui-guo LIU Xue CHEN jun-nan Department of Respiratory and Critical Care Medicine Tongji HospitalTong3i Medical CollegeHuazhong University of Science and Technology Key Laboratory of Respiratory Disease of the Ministry of Health WuhanHubei 430030China

Background Obstructive sleep apnea （OSA） can cause cognitive dysfunction and may be a reversible cause of cognitive loss in patients with Alzheimer＇s disease （AD）. Chronic exposure to intermittent hypoxia （IH）, such as encountered in OSA, is marked by neurodegenerative changes in rat brain. We investigated the change of thioredoxin （Trx）, spatial learning and memory in rats exposed to chronic intermittent hypoxia （CIH）. Methods Forty healthy male Sprague-Dawley （SD） rats were randomly divided into four groups of ten each： a CIH＋normal saline （CIH＋NS group）, a N-acetylcystein-treated CIH （CIH＋NAC） group, a sham CIH group （sham CIH＋NS）, and a sham NAC-treated sham CIH （CIH＋NAC） group. Spatial learning and memory in each group was assessed with the Morris water maze. Real-time PCR and Western blotting were used to examine mRNA and protein expression of Trx in the hippocampus tissue. The terminal deoxynucleotidyl transferase-mediated dUTP-nick end-labeling （TUNEL） method was used to detect the apoptotic cells of the hippocampus CA1 region. Results CIH-rats showed impaired spatial learning and memory in the Morris water maze, including longer mean latencies for the target platform, reduced numbers of passes over the previous target platform and a smaller percentage of time spent in the target quadrant. Trx mRNA and protein levels were significantly decreased in the CIH-hippocampus, meanwhile, an elevated apoptotic index revealed apoptosis of hippocampal neurons of rats exposed to CIH. The rats, which acted better in the Morris water maze, showed higher levels of the Trx mRNA and protein in the hippocampus; apoptotic index of the neurons in the hippocampus of each group was negatively correlated with the Trx mRNA and protein levels. Conclusion The Trx deficit likely plays an important role in the impaired spatial learning and memory in the rats exposed to CIH and may work through the apoptosis of neurons in the hippocampus.

关键词： intermittent hypoxia thioredoxin spatial learning oxidative stress sleep apnea

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Prospective,single-center cohort studyanalyzing the efficacy of complete laparoscopic resection on recurrent hepatocellular carcinoma

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Chinese Journal of Cancer 2016年第5期35卷 229-235页

作者： Jie Zhang Zhong-guo Zhou Zhong-Xi Huang Ke-Li Yang Jian-Cong Chen Jin-Bin Chen Li Xu Min-Shan Chen Yao-jun Zhang Department of Hepatobiliary Oncology Sun Yat-sen University Cancer CenterState Key Laboratory of Oncology in South ChinaCollaborative Innovation Center for Cancer Medicine

Background:Laparoscopic hepatectomy is increasingly being used to treat hepatocellular carcinoma(HCC).However,few studies have examined the treatment of recurrent HCC in patients who received a prior *** present prospective study compared the clinical efficacy of laparoscopic surgery with conventional open surgery in HCC patients with postoperative tumor ***:We conducted a prospective study of 64 patients,all of whom had undergone open surgery once before,who were diagnosed with recurrent HCC between june 2014 and November *** laparoscopic group(n = 31)underwent laparoscopic hepatectomy,and the control group(n = 33) underwent conventional open *** time,intraoperative blood loss,surgical margins,postoperative pain scores,postoperative time until the patient could walk,anal exsufflation time,length of hospital stay,and inpatient costs were compared between the two *** patients were followed up for 1 year after surgery,and relapse-free survival was compared between the two ***:All surgeries were successfully *** conversion to open surgery occurred in the laparoscopic group,and no serious postoperative complications occurred in either *** significant difference in inpatient costs was found between the laparoscopic group and the control group(P = 0.079),but significant differences between the two groups were observed for operation time(116.7 ± 37.5 vs.148.2 ± 46.7 min,P = 0.031),intraoperative blood loss(117.5 ± 35.5 vs.265.9 ± 70.3 mL,P = 0.012),postoperative time until the patient could walk(1.6 ± 0.6vs.2.2 ± 0.8 days,P < 0.05),anal exsufflation time(2.1 ± 0.3 vs.2.8 ± 0.7 days,P = 0.041),visual analogue scale pain score(P < 0.05),postoperative hepatic function(P < 0.05),and length of hospital stay(4.5 ± 1.3 vs.6.0 ± 1.2 days,P = 0.014).During the 1-year postoperative follow-up period,6 patients in each group had recurrent HCC on the side of the initial operation,but no significant difference

关键词：英语教学教学理念英语阅读

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