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Biomedical and Environmental Sciences 2007年第5期20卷 386-391页

作者： JIANG YUE peng WANG YING-HUI LIU JUN-YU WU JIE CHEN ren-xiu peng Department of Pharmacology College of Basic Medicine Wuhan University Wuhan 430071 Hubei China Inspection ＆ Quarantine Technical Center Hubei Entry-exit Inspection and Quarantine Bureau Wuhan 430022 Hubei China

Objective To establish a fast and sensitive method for the detection of 8-hydroxy-2’-deoxyguanosine （8-OHdG） in precision-cut rat liver slices by HPLC-MS/MS and to investigate isoniazid （INH） -induced oxidative DNA damage. Methods Precision-cut liver slices （300 μm） were prepared from male rats, and incubated with INH （0.018 mol/L） for 2 h after 1 h preincubation. DNA in the slices was extracted and digested into free nucleosides at 37℃ . The samples were injected into HPLC-MS/MS after the proteins were removed. The level of oxidative DNA damage was estimated using the ratio of 8-OHdG to deoxyguanosine （dG）. Results The limit of detection of 8-OHdG was 1 ng/mL （S/N=3） and the intra-assay relative standard variation was 3.38% when one transition 284.3/168.4 was used as a quantifier and another two transitions 284.3/140.2, 306.1/190.2 as qualifiers. 8-OHdG and dG were well separated, as indicated by elution at 10.02 and 7.37 min, respectively. INH significantly increased the ratio of 8-OHdG to dG in rat liver slices （P〈0.05）. Conclusion 8-OHdG in precision-cut liver slices could be sensitively determined by HPLC-MS/MS. HPLC-MS/MS coupled with precision-cut tissue slices is a fast and reliable analytical technique to evaluate oxidative DNA damage of target tissues caused by procarcinogens and cytotoxins.

关键词： Isoniazid 8-Hydroxy-2'-deoxyguanosine HPLC-MS/MS Precision-cut liver slices

CYP2E1 mediated isoniazid-induced hepatotoxicity in rats

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Acta Pharmacologica Sinica 2004年第5期25卷 157-162页

作者： Jiang YUE, ren-xiu peng1, Jing YANG, Rui KONG, Juan LIUDepartment of Pharmacology, Medical College of Wuhan University, Wuhan 430071, China Department of Pharmacology Medical College of Wuhan University Wuhan 430071

AIM: To investigate the role of CYP2E1 in isoniazid (INH)-induced hepatotoxicity and the influence of rifampicin(RFP) on INH-induced liver injury. METHODS: Rats were treated with INH alone (100 mg/kg, ip) or co-administeredwith RFP (100 mg/kg, ig) for 10 d and 21 d. Hepatotoxicity was assayed by plasma enzymes (sALT, sAST) andhistopathological examinations. Hepatic CYP2E1 activity was measured by aniline hydroxylase (ANH), and CYP2E1mRNA expression was determined by RT-PCR. Plasma hydrazine concentration was determined by ***: For a 10 d INH-treatment, hepatic CYP2E1 level was increased to 3.7-fold over the control; liverimpairment appeared after 21 d treatment, while CYP2E1 and plasma hydrazine were, respectively, increased to 4.6-fold and 1.7-fold. However, in INH-RFP group for 10 d, CYP2E1 and plasma hydrazine were, respectively,decreased by 13 % and 18 % over INH group; similarly, hepatic injury is equal to INH group appeared after 21 d,and CYP2E1 was further decreased by 26 %. Correlation analysis showed that sALT had a positive correlation withplasma hydrazine and with CYP2E1 activity; CYP2E1 activity was also markedly correlated with plasma *** compared with control, there is no difference in changes of CYP2E1 mRNA expression in INH and INH-RFPtreatment for 21 d. CONCLUSION: The metabolite of INH, hydrazine, plays an important role in INH-inducedhepatotoxicity in rats. The induction of CYP2E1 by hydrazine is involved in the hepatotoxicity of INH. RFP doesnot exacerbate INH-induced hepatotoxicity in short term, which relates to down-regulation of CYP2E1.

关键词： isoniazid rifampin hydrazines cytochrome P-450 CYP2E1

Influences of 3-methylcholanthrene,phenobarbital and dexamethasone on xenobiotic metabolizing-related cytochrome P450 enzymes and steroidogenesis in human fetal adrenal cortical cells

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Acta Pharmacologica Sinica 2006年第8期27卷 1093-1096页

作者： Hui WANG~2 Min HUANG ren-xiu peng Jiang LE Department of Pharmacology,Basic Medical School of Wuhan University,Wuhan 430071,China Department of Pharmacology Basic Medical School of Wuhan University Wuhan China

Aim:To explore the influence and possible mechanism of xenobiotics on adrenalsteroidogenesis during fetal ***:Primary human fetal adrenalcortical cells were prepared,cultured and treated with 3-methylcholanthrene,phe-nobarbital and *** activities of 7-ethoxyresorufin O-dealkylase,benzphetamine,aminopyrine and erythromycin N-demethylases were measuredby enzyme *** the same time,quantitative analysis of steroid hormonescortisol,aldosterone,testosterone and progesterone were carried out in culturalmedium by ***:The activities of benzphetamine andaminopyrine N-demethylase were increased in the cultural fetal adrenal cells treatedwith phenobarbital(0.25-1 mmol/L)for ***(25-100 μmol/L)alsoincreased the activity of erythromycin *** activity of 7-ethoxyresorufin O-dealkylase was undetected in the cells treated without andwith 3-methylcholanthrene(0.5-2μmol/L).Meanwhile,the contents of mediumcortisol,aldosterone and progesterone were decreased after treatment with ***,aldosterone and progesterone concentrations werealso slightly decreased with *** enhanced the pro-ductions of cortisol and progesterone *** trend of testosteroneconcentration was uncertain after 3-methylcholanthrene,phenobarbital or dexam-ethasone ***:3-Methylcholanthrene,phenobarbital or dex-amethasone could interfere with the synthesis of cortisol,aldosterone and proges-terone in primary human fetal adrenal cortical cells,which likely act throughxenobiotic metabolizing-related cytochrome P450 isoform activation.

关键词： xenobiotics steroidogenesis adrenal glands cytochrome P-450 enzyme system fetal development

Probability modeling for robustness of multivariate LQG designing based on ship lateral motion

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Journal of Marine Science and Application 2005年第4期4卷 18-22页

作者： ZHAO Xi-ren peng xiu-yan YIN Zhong-feng School of Automation Harbin Engineering University Harbin 150001 China

The robustness of LQG designing for latitudinal movement of ship is mainly discussed, when its hydrodynamic parameters fluctuate around criterion value at random on the proportional distributing. When a given ship state at the speed of 18 kn and the course of 45° under Rank 5 state of sea, and the hydrodynamic parameters of the ship fluctuate at random on the proportional distributing with a range of ± 10% , ± 20% , ± 30% , the robustness of muhivariate LQG designing for ship is analyzed with applying the probability modeling of relative controlling effect, The result of simulating shows that when the hydrodynamic parameters of ship fluctuates the relative controlling effect of the LQG designing submit to normal distribution and the mean value of relative controlling effect has no remarkable changes comparing to that without perturbation of hydrodynamic parameter,

关键词： hydrodynamic parameters perturbation LQG designing statislic modeling

Preparation of the In_2O_23·Sn Films by MO-CVD Technique

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Chemical Research in Chinese Universities 1992年第4期8卷 477-479页

作者： LUO Wen-xiu, ren peng-cheng and TAN Zhong-ke(Center for Fimctional Materials Rcscarch, Qingdao Institute ofChemical Technology, Qingdao, 266042)LI Zhi-ping (Department of Applied Chemistry, Qingdao University, Qingdao, 266071)

Introduction In;O;·Sn films have high transparency（>95％） within the visible spectral region, low resistivity(10;—10;ohm·cm) at room temperature and superior thermal stability. These films have been applied to solar cells, electronics and photoelectronics fields. In recent years, organometallic-CVD method has emerged as a successful alternate to the physical methods and general CVD for the growth of these films. The MO-CVD tech-

关键词： Metallorganic chemical vapour deposition (MOCVD) Tin doped Indium oxide

宫腔镜下钬激光治疗子宫黏膜下肌瘤的效果及对性激素、性生活质量的影响

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解放军医药杂志 2021年第2期33卷 70-74页

作者：任秀朋崔微微定州市人民医院妇产科河北定州073000

目的探讨宫腔镜下钬激光治疗子宫黏膜下肌瘤的效果及对性激素、性生活质量的影响。方法选取我院妇科2018年1月—2019年5月收治的子宫黏膜下肌瘤88例,按照治疗方法的不同分为观察组51例和对照组37例,观察组给予宫腔镜下钬激光治疗,对照... 详细信息

目的探讨宫腔镜下钬激光治疗子宫黏膜下肌瘤的效果及对性激素、性生活质量的影响。方法选取我院妇科2018年1月—2019年5月收治的子宫黏膜下肌瘤88例,按照治疗方法的不同分为观察组51例和对照组37例,观察组给予宫腔镜下钬激光治疗,对照组给予宫腔镜电切术治疗。比较2组手术时间、术中出血量、术后疼痛程度、术后并发症及术后住院时间、月经恢复正常时间。比较2组术前、术后3个月血清促卵泡生长激素(FSH)、黄体生成素(LH)、雌二醇(E 2)水平,比较2组术前、术后6个月性生活质量评分。结果与对照组比较,观察组手术时间、术后住院时间及月经恢复正常时间显著缩短,术中出血量显著减少,术后视觉模拟疼痛评分显著降低(P0.05)。与术前比较,2组术后6个月各项性功能评分均显著升高,且观察组高于对照组(P<0.05,P<0.01)。结论宫腔镜下钬激光治疗子宫黏膜下肌瘤除具有手术时间短、术中出血量少、疼痛轻、术后恢复快、安全性高的优势外,对患者性激素水平及性生活质量的影响较小。

关键词：肌瘤,子宫钬激光手术宫腔镜电切术手术后并发症卵泡刺激素促黄体激素雌二醇

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应用塑料开口器进行洗胃插管的临床观察

实用护理杂志 2003年第9期19卷 52-53页

作者：曲希莲任秀菊彭志红王晓盖鑫胜利油田中心医院急诊科

多年来,临床上洗胃一直采用开口器(为了便于区分,以下称金属开口器)协助插胃管和在洗胃过程中支撑牙齿防止咬管,但实际应用中发现金属开口器有诸多不便和缺陷,为此,作者在塑料牙垫的启示下,自行设计制作了一种塑料开口器,并与金属开口... 详细信息

多年来,临床上洗胃一直采用开口器(为了便于区分,以下称金属开口器)协助插胃管和在洗胃过程中支撑牙齿防止咬管,但实际应用中发现金属开口器有诸多不便和缺陷,为此,作者在塑料牙垫的启示下,自行设计制作了一种塑料开口器,并与金属开口器进行了对比研究,报告如下.

关键词：塑料开口器洗胃插管观察护理

Influence of slip ratio on worn-surface microstructure and fatigue wear behavior of D2 wheel steel

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Journal of Iron and Steel Research International 2018年第12期25卷 1278-1286页

作者： Chun-peng Liu xiu-juan Zhao peng-tao Liu Chong Xu Rui-ming ren School of Material Science and Engineering Dalian Jiaotong UniversityDalian 116028LiaoningChina Key Laboratory of Critical Materials of Rail Transportation in Liaoning Province Dalian Jiaotong University Dalian 116028LiaoningChina School of Mechanical Engineering Dalian Jiaotong UniversityDalian 116028LiaoningChina

To investigate the worn-surface microstructure and fatigue cracks in D2 wheel steel under the pure rolling and 0.5% slip ratio conditions,a rolling wear test using a GPM-40 wear machine to simulate the wheel/rail operation was performed. After testing,a transmission electron microscope,a scanning electron microscope with electron backscatter diffraction,and micro-hardness testers were used to characterize the microstructure and fatigue *** surface microstrncture and hardness of the pure rolling sample were in a steady state after 8 × 10^4 cycles;however,the 0.5% slip ratio sample reached a steady state after 7 × 10^3 *** of whether the test uses the slip ratio,the orientation of lamellar pearlites gradually became parallel to the surface and a portion of lamellar cementites was fragmented and dissolved during the formation of steady-state *** slip ratio accelerates this *** hardening mechanism of the samples shows a decrease in the lamellar spacing of pearlite and the refinement of proeutectoid ferrite (PF).As the number of cycles increased,plastic deformation of samples became increasingly severe and the wear mechanism of the samples was fatigue wear in steady *** sample surfaces formed shallow cracks,which gradually peeled *** slip ratio accelerated the initiation and propagation of fatigue cracks because of the high friction stress on the contact *** fatigue cracks initiated at the interface of pearlite and PF and in the PF region.

关键词： D2 wheel steel Slip ratio Wom-surface microstructure Wheel/rail system Rolling wear

Sulfolane‑Based Flame‑Retardant Electrolyte for High‑Voltage Sodium‑Ion Batteries

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Nano-Micro Letters 2025年第2期17卷 498-516页

作者： Xuanlong He Jie peng Qingyun Lin Meng Li Weibin Chen Pei Liu Tao Huang Zhencheng Huang Yuying Liu Jiaojiao Deng Shenghua Ye Xuming Yang Xiangzhong ren Xiaoping Ouyang Jianhong Liu Biwei Xiao Jiangtao Hu Qianling Zhang Graphene Composite Research Center College of Chemistry and Environmental EngineeringShenzhen UniversityShenzhen 518060People’s Republic of China Center of Electron Microscopy State Key Laboratory of Silicon and Advanced Semiconductor MaterialsSchool of Materials Science and EngineeringZhejiang UniversityHangzhou 310027People’s Republic of China GRINM(Guangdong)Research Institute for Advanced Materials and Technology FoshanGuangdong 528051People’s Republic of China College of Energy Engineering Zhejiang UniversityHangzhouZhejiang 310027People’s Republic of China School of Materials Science and Engineering Xiangtan UniversityXiangtan 411105People’s Republic of China Shenzhen Eigen-Equation Graphene Technology Co.Ltd Shenzhen 518000People’s Republic of China

Sodium-ion batteries hold great promise as next-generation energy storage ***,the high instability of the electrode/electrolyte interphase during cycling has seriously hindered the development of *** particular,an unstable cathode–electrolyte interphase(CEI)leads to successive electrolyte side reactions,transition metal leaching and rapid capacity decay,which tends to be exacerbated under high-voltage ***,constructing dense and stable CEIs are crucial for high-performance *** work reports localized high-concentration electrolyte by incorporating a highly oxidation-resistant sulfolane solvent with non-solvent diluent 1H,1H,5H-octafluoropentyl-1,1,2,2-tetrafluoroethyl ether,which exhibited excellent oxidative stability and was able to form thin,dense and homogeneous *** excellent CEI enabled the O3-type layered oxide cathode NaNi_(1/3)Mn_(1/3)Fe_(1/3)O_(2)(NaNMF)to achieve stable cycling,with a capacity retention of 79.48%after 300 cycles at 1 C and 81.15%after 400 cycles at 2 C with a high charging voltage of 4.2 *** addition,its nonflammable nature enhances the safety of *** work provides a viable pathway for the application of sulfolane-based electrolytes on SIBs and the design of next-generation high-voltage electrolytes.

关键词： Sodium-ion batteries Sulfolane-based electrolyte High voltage Layered oxide cathode Flame retardant

Defect and interface engineering in core@shell structure hollow carbon@MoS_(2)nanocomposites for boosted microwave absorption performance

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Nano Research 2022年第9期15卷 7778-7787页

作者： Junxiong Xiao Xiaosi Qi xiu Gong Qiong peng Yanli Chen ren Xie Wei Zhong College of Physics Guizhou Province Key Laboratory for Photoelectrics Technology and ApplicationGuizhou UniversityGuiyang 550025China National Laboratory of Solid State Microstructures and Jiangsu Provincial Laboratory for NanoTechnology Nanjing UniversityNanjing 210093China

Defect and interface engineering are efficient approaches to adjust the physical and chemical properties of *** order to effectively utilize these strategies for the improvement of microwave absorption properties(MAPs),in this study,we reported the synthesis of hollow carbon shells and hollow carbon@MoS_(2)nanocomposites by the template-etching and templateetching-hydrothermal methods,*** obtained results indicated that the degree of defect for hollow carbon shells and hollow carbon@MoS_(2)could be modulated by the thickness of hollow carbon shell,which effectively fulfilled the optimization of electromagnetic parameters and improvement of ***,the microstructure investigations revealed that the precursor of hollow carbon shells was encapsulated by the sheet-like MoS_(2)in high *** the introduction of MoS_(2)nanosheets acting as the shell effectively improved the interfacial effects and boosted the polarization loss capabilities,which resulted in the improvement of comprehensive *** elaborately designed hollow carbon@MoS_(2)samples displayed very outstanding MAPs including strong absorption capabilities,broad absorption bandwidth,and thin matching ***,this work provided a viable strategy to improve the MAPs of microwave absorbers by taking full advantage of their defect and interface engineering.

关键词： core@shell structure hollow carbon shells hollow carbon@MoS_(2) defect and interface engineering improved microwave absorption properties