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Prenatal cytogenetic diagnosis study of 2782 cases of high-risk pregnant women

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Chinese Medical Journal 2010年第4期123卷 423-430页

作者： ZHANG Lin ZHANG Xiao-hong LIANG mei-ying ren mei-hong Prenatal Diagnosis Center Peking University People's Hospital Beijing 100044 China

Background Prenatal diagnoses are extremely advantageous for pregnant women with high-risk indicators and can help prevent the birth of malformed infants. However, no large-scale statistical study analyzing the correlation between fetal chromosome disorders and abnormal indicators during pregnancy has been done in China. The objectives of this study were to diagnose and analyze fetal chromosome abnormalities, determine the feasibility of the various prenatal test methods and establish diagnostic guidelines for the early, middle, and late trimesters. Methods From January 2004 to May 2009, 2782 pregnant women at high-risk underwent prenatal diagnoses. Categorized data expressed as either actual counts or percentages were analyzed by the chi-square or Fisher＇s exact test. Chorionic villus sampling was performed in the early-trimester （10-12 weeks of gestation）, amniocentesis in mid-trimester （16-28 weeks of gestation）, and umbilical cord blood collection in mid- or late-trimester （16-37 weeks of gestation）. In 51 cases either autopsy samples from intrauterine fetal deaths or placental tissues from aborted fetuses were tested. Results Chromosomal abnormalities were observed in 3.99% （111/2782） of the samples. Overall, the success rate of cytogenetic analysis for high-risk pregnancy groups was 98.17% （2731/2782）. It was significantly less successful when used to analyze data from the chorionic villus sampling compared with that from amniocentesis and umbilical cord blood （P=-0.000）. Abnormal chromosome carriers had the highest percentage of abnormal chromosomes （67.86%） when compared with chromosomal abnormalities in patients with ultra-sonographic ＂soft markers＂（11.81%）, advanced maternal age （4.51%） and those who had positive serum screening results （P=-0.000）. Conclusions Invasive prenatal diagnostic techniques are feasible tools for confirming fetal chromosomal abnormalities. Abnormal chromosomes detected in one of the parents carrying abnormal chromosome, ultrasoun

关键词： chorionic villus sampling amniocentesis umbilical cord blood collection chromosomal karyotype prenatal diagnosis

Dietary curcumin alleviates intestinal damage induced by ochratoxin A in juvenile grass carp(Ctenopharyngodon idella):Necroptosis and inflammatory responses

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Animal Nutrition 2024年第3期18卷 119-132页

作者： Piao Zhao Wei Dan Jiang Pei Wu Yang Liu Hong mei ren Xiao Wan Jin Lin Feng Xiao Qiu Zhou Animal Nutrition Institute Sichuan Agricultural UniversityChengdu 611130SichuanChina Fish Nutrition and Safety Production University Key Laboratory of Sichuan Province Sichuan Agricultural UniversityChengdu 611130SichuanChina Key Laboratory of Animal Disease-Resistance Nutrition Ministry of EducationMinistry of Agriculture and Rural AffairsKey Laboratory of Sichuan ProvinceSichuan611130China

Ochratoxin A(OTA)is one of the most common pollutants in aquatic *** a first line of defense,intestinal barriers could be utilized against OTA in order to prevent *** product supplementation is one of the most popular strategies to alleviate toxicity induced by mycotoxins,but there is a lack of knowledge about how it functions in the teleost *** this study,720 juvenile grass carp of about 11 g were selected and four treatment groups(control group,OTA group,curcumin[Cur]group,and OTA+Cur group)were set up to conduct a 60-day growth *** the test,the growth performance and intestinal health related indexes of grass carp were *** addition of dietary Cur could have the following main results:(1)inhibit absorption and promote efflux transporters mRNA expression,reducing the residuals of OTA,(2)decrease oxidative stress by reducing oxidative damage and enhancing the expression of antioxidant enzymes,(3)promote mitochondrial fusion proteins to inhibit the expression of mitotic proteins and mitochondrial autophagy proteins and enhance mitochondrial function,(4)reduce necroptosis-related gene expression through inhibiting the tumor necrotic factor receptor-interacting protein kinase/mixed lineage kinase domain-like pathway,(5)reduce the expression of pro-inflammatory factors by inhibiting the Toll-like receptor 4/nuclear factor-κB signaling pathway to alleviate the intestinal inflammatory *** summary,the results suggested that Cur could alleviate OTA-induced intestinal damage by enhancing antioxidant capacity and mitochondrial function as well as reducing necroptosis and inflammation in the grass carp *** study provided a theoretical basis and production implications for dietary Cur that could improve growth performance and alleviate the intestinal damage induced by OTA in fish.

关键词： Curcumin Ochratoxin A Grass carp growth performance Mid-intestine mitochondria function Necroptosis

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裂解C_5中异戊二烯阻聚工艺条件研究

化学工程 2005年第5期33卷 75-78页

作者：钱仁渊王美日史美仁南京工业大学化学化工学院江苏南京210009

在玻璃封管里比较了乙醇胺(C2H7NO)、硝基苯(C6H5NO2)、N-甲基吡咯烷酮(NMP)、邻硝基苯酚(ONP)、亚硝酸钠(NaNO2)对异戊二烯(IP)自由基聚合的阻聚效果,阻聚活性的次序为ONP>NaNO2>NMP>C6H5NO2>C2H7NO。并重点考察了反应温度、反应时间... 详细信息

在玻璃封管里比较了乙醇胺(C2H7NO)、硝基苯(C6H5NO2)、N-甲基吡咯烷酮(NMP)、邻硝基苯酚(ONP)、亚硝酸钠(NaNO2)对异戊二烯(IP)自由基聚合的阻聚效果,阻聚活性的次序为ONP>NaNO2>NMP>C6H5NO2>C2H7NO。并重点考察了反应温度、反应时间、加入量对ONP阻聚作用的影响。结果表明:温度越高,IP聚合速度越快。IP损失率与反应时间呈线性关系。阻聚效果较好的ONP的质量分数范围为0.3%—1.5%。

关键词：异戊二烯邻硝基苯酚阻聚

Characteristics and Driven Factors of Nitrous Oxide and Carbon Dioxide Emissions in Soil Irrigated with Treated Wastewater

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Journal of Integrative Agriculture 2012年第8期11卷 1354-1364页

作者： XUE Yan-dong YANG Pei-ling LUO Yuan-pei LI Yun-kai ren Shu-mei SU Yan-ping NIU Yong-tao College of Water Resources and Civil Engineering China Agricultural University Beijing 100083 P.R.China Institute of Environment and Sustainable Development in Agriculture Chinese Academy of Agricultural Sciences Beijing 100081 P.R. China

The reuse of treated wastewater in agricultural systems could partially help alleviate water resource shortages in developing countries. Treated wastewater differs from fresh water in that it has higher concentrations of salts, Escherichia coli and presence of dissolved organic matter, and inorganic N after secondary treatment, among others. Its application could thus cause environmental consequences such as soil salinization, ammonia volatilization, and greenhouse gas emissions. In an incubation experiment, we evaluated the characteristics and effects of water-filled pore space （WFPS） and N input on the emissions of nitrous oxide （N2O） and carbon dioxide （CO2） from silt loam soil receiving treated wastewater. Irrigation with treated wastewater （vs. distilled water） significantly increased cumulative N2O emission in soil （117.97 μg N kg-1）. Cumulative N2O emissions showed an exponentially increase with the increasing WFPS in unamended soil, but the maximum occurred in the added urea soil incubated at 60% WFPS. N2O emissions caused by irrigation with treated wastewater combined with urea-N fertilization did not simply add linearly, but significant interaction （P〈0.05） caused lower emissions than the production of N2O from the cumulative effects of treated wastewater and fertilizer N. Moreover, a significant impact on cumulative CO2 emission was measured in soil irrigated with treated wastewater. When treated wastewater was applied, there was significant interaction between WFPS and N input on N2O emission. Hence, our results indicated that irrigation with treated wastewater should cause great concern for increasing global warming potential due to enhanced emission of N2O and CO2.

关键词： treated wastewater nitrous oxide carbon dioxide water-filled pore space urea

Molecular Phylogenetic Analysis of the Main Lineages of Nymphalinae(Nymphalidae: Lepidoptera) Based on the Partial Mitochondrial COI Gene

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Agricultural Sciences in China 2008年第6期7卷 731-739页

作者： ZHANG Min CAO Tian-wen ZHONG Yang ren Zhu-mei GUO Ya-ping MA En-bo College of Life Science and Technology Shanxi University Taiyuan 030006 P.R.China Institute of Plant Protection Shanxi Academy of Agricultural Sciences Taiyuan 030031 P.R.China School of Life Sciences Fudan University Shanghai 200433 P.R.China

The phylogenetic relationships of the subfamily Nymphalinae （sensu Chou 1994） were analyzed based on 1 488 bp of mtDNA cytochrome oxidase subunit I （COI） gene sequence data obtained from 24 individuals, along with those of eight species obtained from GenBank. The base compositions of this COI fragment varied among the individuals as follows： T 39.9%, C 14.6%, A 32.2%, and G 13.4%, with a strong AT bias （72.1%）, as usually found in insect mitochondrial genomes. The A ＋T contents of the third, second, and first codon positions of the COI fragments in this study was 92.4, 62.2, and 61.4%, respectively. The phylogenetic trees were reconstructed by neighbor-joining （NJ）, maximum likelihood （ML）, and Bayesian methods by using Byblia anvatara as outgroup. Phylogenetic analyses based on the COI gene sequence data created very similar topologies, which were producing trees with two main clades A and B, and five subclades. The data indicated that the tribes Nymphalini and Hypolimni （sensu Chou 1994） are not monophyletic groups, and the genus Junonia should be removed from Nymphalini to Hypolimni （=Junoniini）. On the basis of the data, the Symbrenthia and Araschnia had a relative distant relationship with the rest of Nymphalini. The relationships of species in the Nymphalini were confirmed via the NJ, ML, and Bayesian methods, namely （（（（Nymphalis ＋ Kaniska）＋ Polygonia）＋Aglais）＋ Vanessa）＋（Symbrenthia ＋Araschnia）. This investigation provides a little novel information for Chinese researches of butterflies.

关键词： Nymphalinae mtDNA molecular phylogeny cytochrome oxidase subunit I gene

Land Use/Land Cover Change(LUCC) and Eco-Environment Response to LUCC in Farming-Pastoral Zone,China

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Agricultural Sciences in China 2009年第1期8卷 91-97页

作者： HAO Hui-mei and ren Zhi-yuan College of Tourism and Environmental Science,Shaanxi Normal University,Xi’an 710062,P.R.China [a]College of Tourism and Environmental Science Shaanxi Normal University Xi'an 710062 P. R. China

In order to understand land use/land cover changes （LUCC） and the eco-environment response to LUCC in farming- pastoral zone of the northern China during the recent twenty years, Baotou prefecture was selected as a case study area for investigation and quantitative evaluation. Technologies of remote sensing （RS）, global positioning system （GPS）, geographic information system （GIS）, and other statistical methods were employed to implement. Results showed that：（1） During the recent twenty years, the areas of forest lands, grasslands and water were reduced, whereas the areas of other types were enlarged. Parts of forest lands, grasslands, and waters had become farmlands, and about 31.5% of the changed grasslands transferred into unused lands. The newly increased farmlands mainly came from grasslands and unused lands. And the newly increased construction lands mainly came from grasslands and farmlands. （2） Regional eco- environmental quality decreased by 12.6%, for which the land degradation （especially the meadow degeneration） and the developing of the cultivated land were mainly responsible, and their contributions to the regional eco-environment changes were 51.84 and 23.63% respectively. （3） The tendency of LUCC and the eco-environment response to LUCC displayed spatial heterogeneity. It can be concluded that the present agricultural production mode was not sustainable in farming-pastoral zone of northern China. Land degradation, especially meadow degradation induced by over-trampling and overgrazing, and developing of cultivated land were mainly responsible for regional eco-environment deterioration. Changing the cultivated land to forest or grass, however, can relieve deterioration of local eco-environment to some extents. And in the farming-pastoral zone in the northern China, evaluating regional eco-environment responses to LUCC was very necessary due to its fragile eco-environments.

关键词： farming-pastoral zone LUCC eco-environment responses to LUCC

Urban-rural Coordinated Development in China from the Perspective of New Urbanization:A Case Study of Jinan City

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Asian Agricultural Research 2015年第8期7卷 44-47页

作者： Kai LIU mei ren Jianlan ren School of Geography and Environment Shandong Normal University

New urbanization has important and practical significance and profound historical significance to the building of a moderately prosperous society in all respects,acceleration of the socialist modernization process and the great rejuvenation of the Chinese nation. And new urbanization emphasizes the coordinated development of urban and rural areas,and it provides new idea for constructing new relationship between urban and rural areas and solving issues of agriculture. This paper sees new urbanization as background,takes Jinan City as an example to study the constraints on coordinated development of urban and rural areas,path choice,urban and rural primary missions. The strategies and measures are finally put forward. This paper will help to solve the problem of the uncoordinated development of urban and rural areas,enrich the research content of urban and rural areas,and provide a reference for other cities.

关键词： New urbanization Urban-rural coordinated developme

Dietary copper improves intestinal structural integrity in juvenile grass carp(Ctenopharyngodon idella)probably related to its increased intestinal antioxidant capacity and apical junction complex

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Animal Nutrition 2024年第3期18卷 96-106页

作者： Rui Ma Lin Feng Pei Wu Yang Liu Hong mei ren Xiao Wan Jin Shu Wei Li Ling Tang Xiao Qiu Zhou Wei Dan Jiang Animal Nutrition Institute Sichuan Agricultural UniversityChengdu611130China Fish Nutrition and Safety Production University Key Laboratory of Sichuan Province Sichuan Agricultural UniversityChengdu611130China Key Laboratory of Animal Disease-Resistance Nutrition Ministry of EducationMinistry of Agriculture and Rural AffairsKey Laboratory of Sichuan ProvinceChengdu611130China Animal Nutrition Institute Sichuan Academy of Animal ScienceSichuan Animtech Feed Co.Ltd.Chengdu610066China

This research evaluated the effects of copper(Cu)on intestinal antioxidant capacity and apical junctional complex(AJC)in juvenile grass carp.A total of 1080 healthy juvenile grass carp(11.16±0.01 g)were fed six diets including different dosages of Cu,namely 0,2,4,6,8 mg/kg(Cu citrate[CuCit]as Cu source)and 3 mg/kg(CuSO_(4)·5H_(2)O as Cu source).The trial lasted for 9 *** findings revealed that dietary optimal Cu supplementation(2.2 to 4.1 mg/kg)promoted intestinal growth,including intestinal length,intestinal length index,intestinal weight,and intestinal somatic index(P>0.05).On the other hand,optimal Cu increased intestinal tight junction(TJ)proteins(except for claudin 15b)and adherens junction(AJ)proteins(E-cadherin,α-catenin,β-catenin,nectin and afadin)mRNA levels(P<0.05),which could be connected to the signaling pathway formed by the Ras homolog gene family,member A(RhoA),Rho-associated kinase(ROCK),and myosin light chain kinase(MLCK).Finally,based on serum indicator D-lactate and intestinal oxidative damage index(ROS),Cu requirement(CuCit as Cu source)for juvenile grass carp from initial weight to final weight(from 11 to 173 g)was determined to be 4.14 and 4.12 mg/kg diet,*** work may provide a theoretical foundation for identifying putative Cu regulation pathways on fish intestinal health.

关键词： Copper Ctenopharyngodon idella Structural integrity Intestine

Expression profiles and function of Toll-like receptors in human corneal epithelia

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Chinese Medical Journal 2007年第10期120卷 893-897页

作者： WU Xin-yi GAO Jian-lu ren mei-yu Department of Ophthalmology Qilu Hospital Shandong University Jinan 250012 China

Background Toll-like receptors play an important role in the human immune system. This study was conducted to investigate the expression profiles and function of Toll-like receptor （TLR）1-9 in human corneal epithelium. Methods The expression of TLR1-9 mRNA in 20 human donor corneal epithelia samples abraded during photorefractive keratotomy （PRK） and cultivated telomerase-immortalized human corneal epithelial cells （THCEs） was examined by semi-quantitative reverse-transcriptase polymerase chain reaction （RT-PCR） analysis. Human peripheral blood mononuclear cells （PBMCs） were used as positive controls. The expression of the TLR2 and TLR4 proteins was detected by Western analysis. ELISA was used to detect IL-8 secretion from THCEs challenged with ligands for TLR3 and TLR4 with and without antibody blockade. Results The expression of TLR1-9 at the mRNA level was detected in the epithelia of 20 patients and in THCE. Significant differences among individuals were observed. One patient was found to lack of the expression of TLR3, 4, 6 and 8, whereas another did not express TLRS. The expression of TLR2 and TLR4 protein was detected in human corneal epithelial cells. As THCE cells express TLR1-9, cells were challenged with lipopolysaccharides （LPS） and poly I：C to determine whether TLR4 and TLR3 were functional. The results showed that secretion of IL-8 by cells stimulated with LPS and Poly I：C was 7 to 10 fold greater than secretion by unchallenged cells. Blocking TLR4 with an anti-TLR4 antibody significantly inhibited the LPS-induced IL-8 production by THCE （P〈0.05）. Conclusion Human corneal epithelial cells express multiple TLRs and are able to recognize LPS and poly I：C. Different expression profiles among individuals suggest that differences in the susceptibilities and sensitivities to bacterial and viral infection in human populations relate to different patterns of TLR expression.

关键词： Toll-like receptors corneal epithelium, human gene expression ligand