检索结果-南通市图书馆

An Overview of PRP-Delivering Scaffolds for Bone and Cartilage Tissue Engineering

Journal of Bionic Engineering 2024年第2期21卷 674-693页

作者： Somayeh Baghersad Behzad Bolandi rana imani Shabnam Afaghi Samira Davoudinia Department of Textile Engineering Amirkabir University of Technology(Tehran Polytechnic)Tehran159163-4311Iran Department of Biomedical Engineering Amirkabir University of Technology(Tehran Polytechnic)Tehran159163-4311Iran

Tissue engineering is nowadays an emerging approach that aims to replace or regenerate diseased or damaged organs with engineered constructs. Considering the key role of growth factors (GFs) in the tissue regeneration process, these biomolecules are considered an important part of the tissue engineering process, so the presence of growth factors in engineered scaffolds can accelerate tissue regeneration by influencing the behavior of cells. Platelet-rich plasma (PRP), as an autologous source of a variety of growth factors, is considered a therapeutic agent for the treatment of degenerative diseases. Regarding its ability to promote the healing process and tissue regeneration, PRP therapy has attracted great attention in bone and cartilage tissue engineering. Incorporating PRP and its derivatives into engineered scaffolds not only bioactivates the scaffold, but the scaffold matrix also acts as a sustained and localized growth factor release system. In addition, the presence of a scaffold can promote the bioactivity of GFs by providing an environment that facilitates their interaction, leading to enhanced effects compared to their free form. This review presents a brief overview of PRP's role in bone and cartilage tissue regeneration with the main focus on scaffold-mediated PRP delivery. In addition, the classification of platelet-rich products, current extraction techniques, terminology, and scaffold bioactivation methods are presented to provide a better understanding of the basics and the key aspects that may affect the effectiveness of therapy in bone and cartilage tissue engineering.

关键词： Platelet-rich plasma Growth factors delivery Scaffold Bone Cartilage

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Optimization and comparison of two different 3D culture methods to prepare cell aggregates as a bioink for organ printing

引用

BIOCELL 2012年第1期36卷 37-45页

作者： rana imani SHAHRIAR HOJJATI EMAMI HOSSEIN FAKHRZADEH NAFISEH BAHEIRAEI ALI M SHARIFI Department of Biomedical Engineering Amirkabir University of TechnologyTehranIran Endocrinology and Metabolism Research Institute Tehran University of Medical SciencesTehranIran Razi Institute for Drug research Department of PharmacologySchool of MedicineTehran University of Medical SciencesTehranIran Department of Tissue Engineering and Cell therapy School of advanced sciences in MedicineTehran University of Medical Sciences.TehranIran

The ultimate goal of tissue engineering is to design and fabricate functional human tissues that are similar to natural cells and are capable of *** of cell aggregates is one of the important steps in 3D tissue engineering technology,particularly in organ *** simple methods,hanging drop(HD)and conical tube(CT)were utilized to prepare cell *** size and viability of the aggregates obtained at different initial cell densities and pre-culture duration were *** proliferative ability of the cell aggregates and their ability to spread in culture plates were also *** both methods,the optimum average size of the aggregates was less than 500μ*** aggregates were smaller than HD aggregates.5,000 cells per drop HD aggregates showed a marked ability to attach and spread on the culture *** proliferative ability reduced when the initial cell density was *** these methods,we found that the HD method having better size controlling ability as well as enhanced ability to maintain higher rates of viability,spreading,and *** conclusion,smaller HD aggregates might be a suitable choice as building blocks for making bioink particles in bioprinting technique.

关键词： tissue engineering hanging drop conical tube bioprinting

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Urine immune profiling by measurement of multiple cytokine/chemokine mRNA levels in renal allograft dysfunction

引用

Open Journal of Immunology 2011年第3期1卷 87-96页

作者： Rubina Naqvi Salma Batool Jafri Zahabia imani Mohammad Mubarak rana Muzaffar 不详

Background: An accurate diagnosis of cause of acute renal graft dysfunction is crucial for the optimal management of transplant recipients. Currently available tests are either insensitive or nonspecific, or are invasive, such as allograft biopsy. During last decade, attempts have been made in search of non invasive markers for the evaluation of cause of graft dysfunction. We studied a set of genes expressed on cytotoxic T Lymphocytes and those related to functioning of regulatory or helper T cells. Methods: We obtained 108 urine samples from 108 renal allograft recipients at the time of graft biopsy done for the evaluation of cause of graft dysfunction. RNA was extracted from urinary cells and messenger RNA (mRNA) encoding perforin, granzyme B (GB), FoxP3, CD3?, CXCR3, TGF-?, CTLA4, PI-9, IL-10, TNF?, T-bet and 18SrRNA measured with the use of quantitative real time polymerase chain reaction (RT-PCR). The levels of expression of genes were correlated with the biopsy findings and the results compared among different groups. Renal allograft biopsies at this institution are performed when there is unexplained rise in serum creatinine of >20% from the baseline value and reported according to Banff classification. SPSS v10.0 used for ***: The mRNA copy numbers of GB, Perforin, FoxP3, CD3, CXCR3, TGF-?, CTL A4, PI9, IL-10, TNF?, and T-bet were log transformed and mean (± SD) levels studied. The expression of all studied genes were compared between ‘nonspecific biopsy findings’ and other specific diagnoses. GB, Perforin, FoxP3, TGF-?, CD3?, CTLA4 CXCR3 and T-bet were higher in acute cellular rejection (ACR), whereas, TGF-? was also found higher in infection, and PI-9 in chronic allograft nephropathy (CAN) and borderline rejection group. Conclusion: Measurement of mRNA levels for genes like GB, Perforin, FoxP3, TGF-β, CD3?, CTLA4, CXCR3 and T-bet in urine samples offers a non invasive means of diag-nosing cause of graft dysfunction.

关键词： Renal Allograft Dysfunction Rejection Cytokines Chemokines

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Urine immune profiling by measurement of multiple cytokine/chemokine mRNA levels in renal allograft dysfunction

引用

Open Journal of Immunology（免疫学期刊） 2011年第3期

作者： Rubina Naqvi Salma Batool Jafri Zahabia imani Mohammad Mubarak rana Muzaffar

来源：评论

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：