检索结果-南通市图书馆

Engineering microparticles based on solidified stem cell secretome with an augmented pro-angiogenic factor portfolio for therapeutic angiogenesis

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Bioactive Materials 2022年第11期7卷 526-541页

作者： Thomas Spater Marisa Assunçao Kwok Keung Lit Guidong Gong Xiaoling Wang Yi-Yun Chen Ying Rao Yucong Li Chi Him Kendrick Yiu Matthias W.Laschke Michael D.Menger Dan Wang Rocky S.Tuan Kay-Hooi Khoo Michael raghunath Junling Guo Anna Blocki Institute for Clinical&Experimental Surgery Saarland UniversityHomburgSaarGermany School of Biomedical Sciences Faculty of MedicineThe Chinese University of Hong KongShatinNew TerritoriesHong KongChina Institute for Tissue Engineering and Regenerative Medicine The Chinese University of Hong KongShatinNew TerritoriesHong KongChina BMI Center for Biomass Materials and Nanointerfaces College of Biomass Science and EngineeringSichuan UniversityChengduSichuan610065China Bioproducts Institute Departments of Chemical and Biological EngineeringThe University of British ColumbiaVancouverBCCanada Academia Sinica Common Mass Spectrometry Facilities for Proteomics and Protein Modification Analysis and Institute of Biological ChemistryAcademia SinicaNankangTaipeiChina Shun Hing Institute of Advanced Engineering(SHIAE) Faculty of EngineeringThe Chinese University of Hong KongShatinNew TerritoriesHong KongChina Ministry of Education Key Laboratory for Regenerative Medicine School of Biomedical SciencesThe Chinese University of Hong KongShatinNew TerritoriesHong KongChina Department of Orthopaedics&Traumatology The Chinese University of Hong KongShatinNew TerritoriesHong KongHong Kong Special Administrative Region of China Institute for Chemistry and Biotechnology Zurich University of Applied SciencesWadenswilSwitzerland State Key Laboratory of Polymer Materials Engineering Sichuan UniversityChengduSichuan610065China

Tissue (re)vascularization strategies face various challenges, as therapeutic cells do not survive long enough in situ, while the administration of pro-angiogenic factors is hampered by fast clearance and insufficient ability to emulate complex spatiotemporal signaling. Here, we propose to address these limitations by engineering a functional biomaterial capable of capturing and concentrating the pro-angiogenic activities of mesenchymal stem cells (MSCs). In particular, dextran sulfate, a high molecular weight sulfated glucose polymer, supplemented to MSC cul-tures, interacts with MSC-derived extracellular matrix (ECM) components and facilitates their co-assembly and accumulation in the pericellular space. Upon decellularization, the resulting dextran sulfate-ECM hybrid material can be processed into MIcroparticles of SOlidified Secretome (MIPSOS). The insoluble format of MIPSOS protects protein components from degradation, while facilitating their sustained release. Proteomic analysis demonstrates that MIPSOS are highly enriched in pro-angiogenic factors, resulting in an enhanced pro-angiogenic bioactivity when compared to naïve MSC-derived ECM (cECM). Consequently, intravital microscopy of full-thickness skin wounds treated with MIPSOS demonstrates accelerated revascularization and healing, far superior to the ther-apeutic potential of cECM. Hence, the microparticle-based solidified stem cell secretome provides a promising platform to address major limitations of current therapeutic angiogenesis approaches.

关键词： Dextran sulfate Extracellular matrix Mesenchymal stem cells Therapeutic angiogenesis Wound healing Poly-electrolyte-driven co-assembly

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Ubuntu in Action

ChinAfrica 2024年第1期16卷 56-56页

作者： KASHVEER raghunath 不详

In South Africa,there is a saying,“I am because we are!”This ancient philosophy called ubuntu explains that our humanity is enriched through shared experiences,compassion and a sense of responsibility to ***,the concepts of harmony,interconnectedness and mutual respect are deeply ingrained in the philosophy,values and culture of the Chinese people.

关键词： Ubuntu Action harmony

通过羰基化、加氢甲酰化及同系物化反应生产煤基和生物质基的化工原料

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

煤炭转化 1988年第2期 38-48页

作者： P.D.Sunavala B.raghunath 林明辉印度工学院化工系印度工学院化工系

1973年10月的石油危机表明,化学工业若只依靠单一原料（原油）是不适宜的。自那时以后,人们就在寻找其它的替代能源和原料,例如:煤、油页岩、焦油砂和生物质等。在本文中,基本上由一氧化碳和氢气混合物组成的合成气将占有重要地位,这是... 详细信息

1973年10月的石油危机表明,化学工业若只依靠单一原料（原油）是不适宜的。自那时以后,人们就在寻找其它的替代能源和原料,例如:煤、油页岩、焦油砂和生物质等。在本文中,基本上由一氧化碳和氢气混合物组成的合成气将占有重要地位,这是因为它可以从任何碳源制取。例如从甲烷到粪肥以及从矿化燃料到农场废料都可作为生产合成气的原料。合成气的可能应用包括通过费-托合成转化成液体燃料以及工业生产各种有机化工原料,如甲醇和乙酸等。另外一些应用还刚刚处在工业化的开始阶段。虽然对一些传统的利用方法（如生产甲醇和液体燃料）已熟知,而人们更明显地感觉到由近代羰基化、加氢甲酰化和同系物化反应构成的C1—Cx化学这个新领域的影响,同为这些反应可产生大量新的化工原料,例如

关键词：化学方程式反应机理甲醇活性催化剂乙酸乙醋反应机制一元醇助催化剂乙烯基乙酸煤基化工原料羰基化生物质

<i>In Vitro</i>Plant Regeneration of <i>Morus indica</i>L. cv. V1 Using Leaf Explant

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

American Journal of Plant Sciences 2013年第10期4卷 2001-2005页

作者： Melur Kodandaram raghunath Karaba N. Nataraja Jyothi Sainath Meghana Radha Sivarajan Sajeevan Mala V. Rajan Syed Mashayak Hussaine Qadri Central Sericultural Research and Training Institute Srirampura Mysore India Department of Crop Physiology University of Agricultural Sciences GKVK Bangalore India.

Adventitious bud induction and plantlet regeneration were studied in a popular mulberry variety, V1 using leaf as an explant. Fully expanded leaf explants were cultured on Murashige and Skoog’s (MS) medium supplemented with thidiazuron (TDZ) (0.5-4.0 mg/l), 6-benzylaminopurine (BAP) (0.5-2.0 mg/l), indole acetic acid (IAA) (2.0 mg/l), gibberlic acid (GA3) (1.0-2.0 mg/l) silver nitrate (AgNO3) (2.0 mg/l) and different carbon sources such as sucrose, fructose and glucose (10%-30%) either individually or in combination to induce adventitious buds and regeneration. The highest percentage (63%) of adventitious bud formation and regeneration (68%) was achieved in the medium containing MS with TDZ (1.0 mg/l), IAA (2.0 mg/l) and AgNO3 (2.0 mg/l). For subsequent regeneration and shoot elongation the MS medium having BAP (1.0 mg/l), GA3 (2.0 mg/l) and AgNO3 (2.0 mg/l) was found to be suitable. Amongst the carbon sources tested, the most suitable carbon source was found to be sucrose (3%) followed by fructose (2%) for adventitious bud formation. Excised in vitro shoots were rooted (60%-80%) in half strength MS medium supplemented with indole-3-butyric acid (1.0 mg/l). The well rooted plantlets were hardened in soil + sand + farm yard manure (FYM) mixture with a success rate of 70%-90%. Since in vitro regeneration is highly genotype-dependent in mulberry, the standardized protocol can be effectively used for further improvement of this leading genotype using biotechnological approaches.

关键词： In Vitro Regeneration Adventitious Bud Thidiazuron Mulberry cv. V1 Carbon Source

胃食管反流病患者幽门螺杆菌的阳性率:系统性综述

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

英国医学杂志中文版 2003年第4期6卷 253-253页

作者： Anna raghunath A Pali S Hungin David Wooff Susan Childs 周丽雅

研究目的:确定胃食管反流病患者幽门螺杆菌阳性率。研究设计:系统评估有关胃食管反流患者与非反流患者幽门螺杆菌阳性率的研究报告。

关键词：胃食管反流病幽门螺杆菌感染率电子数据库